Effect of Different Level of Monensin Supplemented with Cold Process Urea Molasses Mineral Block on In vitro Rumen Fermentation at Different Days of Adaptation with Monensin

De, Debasis,Singh, G.P. Asian Australasian Association of Animal Productio 2005 Animal Bioscience Vol.18 No.3

Effect of period of adaptation and levels of monensin were studied for microbial fermentation/ digestibility to find out the optimum period of adaptation of monensin in rumen and suitable level of monensin in wheat straw+concentrate and wheat straw+UMMB diet. The mean digestibility of dry matter was decreased upto T-3 treatment (49.17%), however, digestibility of DM was affected upto period (P-2). NDF digestibility was affected due to treatment under P1 and P2 (p<0.05). Average digestibility of ADF was increased to 53.33% at T-3 level of monensin and P4 days of adaptation. TVFA (mmole/100 ml) were decreased from 9.49 in T-1 to 7.70 in T-7. Periods were not effectives except P2 (14 days of adaptation). Similarly, total gas was decrease with the increase of monensin levels in diet. Although acetate percentage in TVFA was not affected either due to level of monensin or period of adaptation but propionate was increased due to increase in monensin at 21 days of adaptation (P-3). Butyrate (%) was decreased significantly in T-2 to T-6 as compared to T-1 group. Total gas was significantly (p<0.01) higher in group T-1 (control) and it reduced significantly in T-5, however, differences in gas production between group T-3, T-5 and T-7 at P-1 was not significant. Methane production was reduced on P-3 and P-4 level of adaptation due to treatment. The overall result indicated that 21 days of adaptation with monensin was sufficient to mask the inhibiting effect of monensin to cell wall digestibility and 35 ppm monensin is optimum to reduce methane production and increase propionate productions.

Effect of Ionophore Enriched Cold Processed Mineral Block Supplemented with Urea Molasses on Rumen Fermentation and Microbial Growth in Crossbred Cattle

De, Debasis,Singh, G.P. Asian Australasian Association of Animal Productio 2003 Animal Bioscience Vol.16 No.6

An experiment was conducted to study the effect of ionophore enriched cold processed mineral block supplemented with urea molasses on microbial growth and rumen fermentation. Twelve adult male crossbred cattle were divided into four groups on body weight basis. Animals were given wheat straw as a basal diet. The animals of group I and II were supplemented with concentrate mixture and animals of group III and IV were supplemented with cold processed urea molasses mineral block (UMMB). Thirty mg monensin/day/animal were supplemented to the animals of group II and 35 ppm monensin were incorporated in the UMMB supplemented to the animals of group IV. Dry matter (DM) intake did not differ significantly among groups. Mean rumen pH was higher in UMMB fed animals. Total volatile fatty acids (TVFA) concentration (mmole/L strained rumen liquor (SRL) in group III (113.19) was significantly (p<0.05) higher than those of group I (105.83) and II (108.74) but similar to group IV (109.34). TVFA production (mole/day) was similar in all the groups. The molar proportion of acetate was significantly (p<0.01) higher in the group I (59.56) than those of group II (51.73) and IV (55.91) but similar to group III (57.12). The molar proportion of propionate was significantly (p<0.01) higher in the monensin treated groups i.e. group II (38.38) and IV (36.26) than those of group I (27.78) and III (33.06). Butyrate molar percent was significantly (p<0.01) higher in group I (12.65) than those of group II (10.19), group III (9.83) and IV (7.84). The reduction of acetate and butyrate was due to UMMB and monensin resulted in lower A:P ratio. Average bacterial pool and bacterial production rate did not differ significantly among groups. Total N concentration (mg/100 ml SRL) was significantly (p<0.01) higher in the group I (55.30) and III (57.70) as compared to the group II (47.97) and IV (47.59). Ammonia-N concentration (mg/100 ml SRL) of group III (34.99) was significantly (p<0.01) higher than that of the group I (25.76) which was again significantly (p<0.01) higher than that of the group II (20.79) and IV (19.83) indicating slower release of ammonia due to monensin in diet. Total bacterial, cellulolytic, proteolytic bacterial and fungal count at 4 h post feeding did not differ significantly (p<0.05) among treatment groups. However, methanogenic bacterial count was significantly (p<0.01) higher in the group I (11.80) compared to the group II (8.43) which was significantly (p<0.01) higher than that of the group III (4.70) and IV (2.90). Average protozoal population was affected by both treatments. Thus feeding of UMMB and monensin in diet affected the rumen fermentation pattern towards propionate production, slower release of ammonia and reduction in methanogenic bacteria in the rumen.

Monensin Enriched Urea Molasses Mineral Block on Feed Intake, Nutrient Digestibility and Blood Glucose in Cattle Fed on Wheat Straw Based Diet

De, Debasis,Singh, G.P. Asian Australasian Association of Animal Productio 2002 Animal Bioscience Vol.15 No.11

Twelve adult male crossbred (Sahiwal${\times}$Holstein Friesian) cattle were distributed into four groups of three each on body weight basis. Animals were given wheat straw as a basal diet. The animals of group I and II were supplemented with concentrate mixture and animals of group III and IV were supplemented with cold processed urea molasses mineral block (UMMB). Thirty mg monensin/day/animal was supplemented to the animals of group II and 35 ppm monensin were incorporated in the UMMB supplemented to the animals of group IV. Vit.A and D mixture was given to all the animals once a week. Dry matter (DM) intake (kg/d) through wheat straw was 19.0 percent higher in the UMMB (without monensin) supplemented group (group III) than those of the concentrate mixture (without monensin) supplemented group i.e. group I. Total DM intake (kg/d) was lower in the monensin supplemented groups than those of non-supplemented groups though differences were not statistically significant. Digestible dry matter, organic matter (OM), crude protein (CP) and total digestible nutrients (TDN) intake were similar in all the groups. Average block consumption for 45 d period in the group III (0.95 kg/d) and group IV (0.84 kg/d) did not differ significantly. DM digestibility (%) was significantly (p<0.01) higher in the group II (58.9) as compared to the group I (52.7) and group III (54.0) but similar to the group IV (57.2). OM digestibility was also significantly (p<0.05) higher in the group II (63.2) as compared to that of the group I (54.9) but similar to the group III (57.8) and IV (59.2). Ether extract (EE) digestibility was significantly (p<0.01) higher in the group I (76.9) and II (80.3) as compared to the group III (59.87) and IV (55.77). Nitrogen free extract (NFE) digestibility was significantly (p<0.05) higher in the group II (62.38) as compared to that of the other groups. Crude protein (CP) and crude fibre (CF) digestibilities were not affected either due to UMMB or monensin. Nitrogen balance did also not differ significantly among the groups. However, Ca and P balance (g/d) in the group III (3.1, 1.3) and IV (3.0, 1.4) were significantly (p<0.01) higher than those of the group I (0.6, 0.2) and II (0.4, 0.3). Blood glucose (mg/100ml) was significantly (p<0.01) higher in the group II (65.2) and IV (65.2) as compared to the group I (55.2) and group III (53.9). Plasma urea-N level (mg/100 ml) in the group III (19.0) and IV (17.8) were significantly (p<0.01) higher than that of the group I (10.5) and II (12.3). So, monensin supplementation either with cold process UMMB or concentrate mixture did not show any additional effect on feed intake and digestibility but increases blood glucose level in adult cattle.

Probing the Thermodynamic and Magnetic Properties of UV-B-Emitting GdAlO3 Phosphors by ESR and Optical Techniques

Singh, V.,Sivaramaiah, G.,Mohapatra, M.,Rao, J. L.,Singh, N.,Pathak, M. S.,Singh, P. K.,Dhoble, S. J. Springer Science + Business Media 2017 Journal of Electronic Materials Vol.46 No.2

<P>GdAlO3 phosphor samples have been prepared by a solution combustion method. X-ray diffraction analysis revealed that the GdAlO3 phosphor stabilized in orthorhombic phase. The optical absorption spectrum showed bands due to Gd3+ ions as well as defect level in the host. The excitation spectrum exhibited a dominant band with maximum at 273 nm. Upon excitation at 273 nm, ultraviolet-B (UV-B) emission with maximum at 312 nm was observed from the undoped sample, being attributed to P-6(7/2) -> S-8(7/2) transition of Gd3+ ion. The UV-B emission was enhanced at liquid-nitrogen temperature (77 K), most probably due to increased ground-state population according to the Boltzmann distribution law. Electron spin resonance spectra of samples at both room and liquid-nitrogen temperature exhibited resonance signals at g ae 2, attributed to Gd3+ ions arranged with octahedral symmetry linked via oxygen bridges. Various magnetic and thermodynamic parameters for the phosphor system were evaluated by standard procedures. Correlation of these values with the optical properties suggested that the GdAlO3 phosphor can be effectively used as an UV-B light source for industrial applications at room temperature.</P>

Estimation of Gene Effects in Four Bivoltine Silkworm (Bombyx mori L.) Crosses

G. N. Malik,T. P. Singh,S. Z. Haque. Rufaie,M. Aijaz,H. U. Dar 한국잠사학회 2004 International Journal of Industrial Entomology Vol.8 No.1

Six generations (P1, P2, F1, BC1, BC2 and F2) of four bivoltine silkworm crosses (SKAU-R-1 ´ Yakwei, SKAU-R-6 ´ Yakwei, CSR2 ´ CSR4 and SH6 ´ NB4D2) were evaluated in a completely randomized block design with 5 replications for each treatment. The generation mean 2 in respect of 3 metric traits (single cocoon weight, single shell weight, and shell ratio %), were subjected to Cavallis joint scaling test. Additive dominance model was found to be adequate in CSR2 ´ CSR4 and SH6 ´ NB4D2 for single cocoon weight and shell weight and SKAU-R-6 ´ Yakwei for shell ratio (%). Whereas, in rest of the crosses epistasis was evident in the traits under investigation. Magnitude of additive gene effect (d) was greater than dominance (h) in SH6 ´ NB4D2 and SKUA-R-6 ´ Yakwei for shell ratio (%) and in CSR2 ´ CSR4 for shell weight. Thus selection for these traits in early segregating generations of these crosses would be effective for obtaining considerable genetic gain.

Studies on Tolerance Level Against Antheraea mylitta Cytoplasmic Polyhedrosis Virus (AmCPV) in Different Eco-races of Tropical Tasar Silkworm, Antheraea Mylitta Drury

Singh, G.P.,Zeya, S.B.,Srivastava, A.K.,Ojha, N.G.,Suryanarayana, N. Korean Society of Sericultural Science 2008 International Journal of Industrial Entomology Vol.17 No.1

Pathogenic infection in tasar silkworm, Antheraea mylitta Drury is common and there is a breed specific response regarding tolerance. Hence, the tolerance level of three eco-races of tasar silkworm viz. Daba, Sarihan and Raily to Antheraea mylitta cytoplasmic polyhedrosis virus (AmCPV) infection was tested. The survival of Daba, Sarihan and Raily eco-races was significantly different (p<0.05) when challenged with the same concentration ($1{\times}10^5$ polyhedra/ml) of AmCPV. Daba eco-race was more tolerant to the AmCPV infection having higher survival (65.7%) and $LC_{50}$ values (1000893.17 polyhedra/ml) of AmCPV followed by Sarihan eco-race (50.7% survival and $LC_{50}$ value of AmCPV 187203.6168 polyhedra/ml) and Raily eco-race (24.3% survival and $LC_{50}$ value of AmCPV 5176.37 polyhedra/ml). The tolerance level of Daba and Sarihan eco-races against AmCPV was more than two times than Raily eco-race.

Studies on Tolerance Level Against Antheraea mylitta Cytoplasmic Polyhedrosis Virus (AmCPV) in Different Eco-races of Tropical Tasar Silkworm, Antheraea Mylitta Drury

( G. P. Singh ),( S. B. Zeya ),( A. K. Srivastava ),( N. G. Ojha ),( N. Suryanarayana ) 한국잠사학회 2008 International Journal of Industrial Entomology Vol.17 No.1

Pathogenic infection in tasar silkworm, Antheraea mylitta Drury is common and there is a breed specific response regarding tolerance. Hence, the tolerance level of three eco-races of tasar silkworm viz. Daba, Sarihan and Raily to Antheraea mylitta cytoplasmic polyhedrosis virus (AmCPV) infection was tested. The survival of Daba, Sarihan and Raily eco-races was significantly different (p<0.05) when challenged with the same concentration (1 x 10(5) polyhedra/ml) of AmCPV. Daba eco-race was more tolerant to the AmCPV infection having higher survival (65.7%) and LC50 values (1000893.17 polyhedra/ml) of AmCPV followed by Sarihan eco-race (50.7% survival and LC50 value of AmCPV 187203.6168 polyhedra/ml) and Raily ecorace (24.3% survival and LC50 value of AmCPV 5176.37 polyhedra/ml). The tolerance level of Daba and Sarihan eco-races against AmCPV was more than two times than Raily eco-race.

Note : Estimation of Gene Effects in Four Bivoltine Silkworm (Bombyx mori L.) Crosses

( G. N. Malik ),( T. P. Singh ),( S. Z. Haque Rufaie ),( M. Aijaz ),( H. U. Dar ) 한국잠사학회 2007 International Journal of Industrial Entomology Vol.8 No.1

Six generations (P1, P2, F1, BC1, BC2 and F2) of four bivoltine silkworm crosses (SKAU-R-1×Yakwei, SKAU-R-6×Yakwei, CSR2×CSR4 and SH6×NB4D2) were evaluated in a completely randomized block design with 5 replications for each treatment. The generation mean 2 in respect of 3 metric traits (single cocoon weight, single shell weight, and shell ratio %), were subjected to Cavallis joint scaling test. Additive dominance model was found to be adequate in CSR2×CSR4 and SH6×NB4D2 for single cocoon weight and shell weight and SKAU-R-6×Yakwei for shell ratio(%). Whereas, in rest of the crosses epistasis was evident in the traits under investigation. Magnitude of additive gene effect (d) was greater than dominance (h) in SH6×NB4D2 and SKUA-R-6×Yakwei for shell ratio (%) and in CSR2×CSR4 for shell weight. Thus selection for these traits in early segregating generations of these crosses would be effective for obtaining considerable genetic gain.

Effect of Different Levels of Rumensin in Diet on Rumen Fermentation, Nutrient Digestibility and Methane Production in Cattle

Singh, G.P.,Mohini, M. Asian Australasian Association of Animal Productio 1999 Animal Bioscience Vol.12 No.8

Twelve rumen fistulated cross-bred calves were divided into three groups and fed wheat straw and concentrate mixture according to their maintenance requirement. Animals in group II and III were fed 50 and 100mg rumensin per day, in addition to basal feed. Supplementation of rumensin in the diet decreased the dry matter intake significantly (p<0.05) along with a significant decrease in the straw intake. Digestibility coefficients of all the nutrients were not affected significantly except that of CF digestibility which was lower (p<0.05) in groups II and III as compared to group I. Among N-parameters in the rumen fluid, mean $NH_3-N$ was significantly lower in groups II and III (19.13 and 18.63 mg N/100 ml respectively) than in group I (22.68); total-N and TCA-ppt-N did not differ among the three groups. Total VFA concentration did also not differ among the three groups, however, propionate increased from 24.33 molar % to 32.73 while acetate and butyrate decreased respectively from 65.85 to 58.81% and 9.79 to 8.46%. Total VFA, bacteria and protozoa production rates were not affected significantly due to rumensin in diet. Methane production per kg DDM as well as % of methane in total gas were reduced at both the levels of rumensin on different concentrate ratios with wheat straw as roughage. Similar trend was also observed with rice straw and concentrate mixture as substrate with rumensin addition.

Breakthrough behaviour of activated charcoal cloth samples against oxygen analogue of sulphur mustard

G. K. Prasad,J. Praveen Kumar,P. V. R. K. Ramacharyulu,Beer Singh 한국탄소학회 2015 Carbon Letters Vol.16 No.1

The breakthrough behaviour of activated charcoal cloth samples against an oxygen analogue (OA) of sulphur mustard has been studied using the modified Wheeler equation. Activated charcoal cloth samples having different surface area values in the range of 481 to 1290 m2/g were used for this purpose. Breakthrough behaviour was found to depend on the properties of the activated charcoal cloth, properties of the OA and the adsorption conditions. Activated charcoal cloth with a high surface area of 1290 m2/g, relatively large surface density of 160 g/m2 and coarser fiber structure exhibited better kinetic saturation capacity value, 0.19 g/g, against OA vapours when compared to others, thus confirming its potential use in foldable masks for protection against chemical warfare agents.