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Fei Fang, Fei,Hye Kim, Ji,Jin Choi, Hyoung,Seo, Yongsok Wiley Subscription Services, Inc., A Wiley Company 2007 Journal of applied polymer science Vol.105 No.4
<P>Barium titanate (BaTiO<SUB>3</SUB>) inorganic particles which possess large electronic resistance and excellent dielectric properties were employed to synthesize conducting polyaniline (PANI)/BaTiO<SUB>3</SUB> composites via an in situ oxidative polymerization, since conducting PANI/inorganic composites have been considered as a superior candidate of electrorheological (ER) fluids because of their physical properties, unique structure, and the combined merits of the two phases. The influence of the fraction of BaTiO<SUB>3</SUB> particles in the as-synthesized composites on the physical properties (morphology and crystal structure) and the ER behaviors were examined. Yield stress data obtained were analyzed based on the universal yield stress equation as a function of applied electric field and it was found that the universal yield stress equation collapses these data onto a single curve independent of BaTiO<SUB>3</SUB> particle concentration. Their shear stresses under an applied electric field were also found to be fitted well with the Cho–Choi–Jhon model. In addition, the investigated dielectric spectra were found to be useful to interpret the differences in the ER performances for the PANI/BaTiO<SUB>3</SUB> composite based ER fluids. © 2007 Wiley Periodicals, Inc. J Appl Polym Sci 2007</P>
Jin, Xin,Zhang, Ke-Jin,Guo, Xu,Myers, Ronald,Ye, Zhong,Zhang, Zhi-Pei,Li, Xiao-Fei,Yang, Hu-Shan,Xing, Jin-Liang Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.17
Over-expression of de novo lipogenesis (DNL) genes is associated with the prognosis of various types of cancers. However, the effects of single nucleotide polymorphisms (SNPs) in these genes on recurrence and survival of non-small cell lung cancer (NSCLC) patients after surgery are still unknown. In this study, a total of 500 NSCLC patients who underwent surgery treatment were included. Eight SNPs in 3 genes (ACACA, FASN and ACLY) of the DNL pathway were examined using the Sequenom iPLEX genotyping system. Multivariate Cox proportional hazards regression and Kaplan-Meier curves were used to analyze the association of SNPs with patient survival and tumour recurrence. We found that two SNPs in the FASN gene were significantly associated with the recurrence of NSCLC. SNP rs4246444 had a significant association with lung cancer recurrence under additive model (hazard ratio [HR], 0.82; 95% confidence interval [95%CI], 0.67-1.00; p=0.05). Under the dominant model, rs4485435 exhibited a significant association with recurrence (HR, 0.75; 95%CI, 0.56-1.01; p=0.05). Additionally, SNP rs9912300 in ACLY gene was significantly associated with overall survival in lung cancer patients (HR, 1.41; 95%CI, 1.02-1.94, p=0.04) under the dominant model. Further cumulative effect analysis showed moderate dose-dependent effects of unfavorable SNPs on both survival and recurrence. Our data suggest that the SNPs in DNL genes may serve as independent prognostic markers for NSCLC patients after surgery.
Miraculin gene transformation in embryogenic Citrus callus and regeneration
Fei Li,Seong Beom Jin,Myeung-Seung Kim,Song-I Han,Hyeon-Jin Sun,Jae Hoon Kim 한국육종학회 2014 한국육종학회 심포지엄 Vol.2014 No.07
Miraculin can modify a sour taste into a sweet taste and is an alternative to traditional sweeteners. Mirculin gene was introduced into the Miyagawa Wase Satsuma mandarin (Citrus unshiu Marc.) callus by Agrobacterum-mediated transformation to produce citrus transgenic plant. Transgenic plant candidates were selected via hygromycin resistance. Transformation was confirmed by Polymerase Chain Reaction, Southern blotting, and Western blot analysis. Expression of this gene in transgenic citrus resulted in the accumulation of miraculin protein in the leaves. Multiple Shoots of transgenic citrus planets were micrografted onto trifoliate rootstocks in the sterile soil. Plants were established in the greenhouse 2 years after planting.
Aeromonas hydrophila cytosolic 5’-methylthioadenosine/S-adenosylhomocysteine nucleosidase MtaN-2
Jinli Chen,Fei Shang,Lulu Wang,Wei Liu,Yuanyuan Chen,Jing Lan,Liming Jin,Nam-Chul Ha,Chunshan Quan,Yongbin Xu 한국구조생물학회 2018 Biodesign Vol.6 No.3
5’-methylthioadenosine/S-adenosylhomocysteine nucleosidase (MTAN) plays a critical role in diverse pathways in bacterial cells such as biological methylation, polyamine biosynthesis, methionine recycling, and bacterial quorum sensing. It has been known that MtaN catalyzes the hydrolysis of N-ribosidic bond of adenosine-based substrates such as S-adenosyl-L-homocysteine (SAH), S-methyl-5’-thioadenosine (MTA) and 5’-deoxyadenosine (5’-DOA). In Aeromonas hydrophila, there are two MtnN subfamily proteins: MtaN-1, a periplasmic protein with an N-terminal signal peptide; and MtaN-2, a cytosolic protein. In this study, MtaN-2 from A. hydrophila was successfully expressed and purified using Ni-NTA affinity, Q anion-exchange, and gel-filtration chromatography. We first crystallized apo MtaN-2 but it diffracted to a low resolution of 5.1 Å. New crystals suitable for diffraction were obtained by adding 2 mM adenosine, a substrate analog of MtaN-2 during purification process and the crystals diffracted to the resolution of 2.0 Å. The crystals belong to the trigonal space group P31 or P32, with unit-cell parameters of a = b = 74.94 Å and c = 185.21 Å. The asymmetric unit contains four complexes of MtaN-2 with hydrolysis products of adenosine.
Fei Shang,Jinli Chen,Lulu Wang,Yuanyuan Chen,Jing Lan,Wei Liu,Liming Jin,하남철,Chunshan Quan,Yongbin Xu 한국구조생물학회 2019 Biodesign Vol.7 No.1
Sirtuins are NAD+-dependent deacetylase that are broadly conserved throughout bacteria, archaea, and eukaryotes. Themembers of sirtuins are important in regulating diverse biological pathways, including gene silencing, DNA repair, genomestability, longevity, metabolism, and cell physiology. Sirtuin from Bacillus amyloliquefaciens (BaSrtN) is a particularlyinteresting bacterial Sir2 homologue. In this study, to further understand the function and mechanisms of this protein,BaSrtN was successfully expressed and purified using Ni-NTA affinity, Q anion-exchange, and gel-filtration chromatography. Purified BaSrtN was crystallized and diffracted to the resolution of 1.45 Å. The preliminary crystallographic analysissuggested that BaSrtN crystal belongs to the trigonal space group P31 or P32, with unit-cell parameters of a = b = 90.115and c = 86.306 Å. Size-exclusion chromatography suggested that BaSrtN prefer to exit as monomers in solution.