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Gui Fang-Fang,Jiang Ge-Ge,Bin Dong,Zhong Shi-Wei,Xiao Zheng,Qiu Fang,Wang Yi-Guang,Yang Li-Yuan,Zhao Hongbo 한국유전학회 2023 Genes & Genomics Vol.45 No.9
Background MIKC type MADS-box transcription factors are one of the largest gene families and play a pivotal role in flowering time and flower development. Chimonanthus salicifolius belongs to the family Calycanthaceae and has a unique flowering time and flowering morphology compared to other Chimonanthus species, but the research on MIKC type MADS-box gene family of C. salicifolius has not been reported. Objective Identification, comprehensive bioinformatic analysis, the expression pattern of MIKC-type MADS-box gene family from different tissues of C. salicifolius. Methods Genome-wide investigation and expression pattern under different tissues of the MIKC-type MADS-box gene family in C. salicifolius, and their phylogenetic relationships, evolutionary characteristics, gene structure, motif distribution, promoter cis-acting element were performed. Results A total of 29 MIKC-type MADS-box genes were identified from the whole genome sequencing. Interspecies synteny analysis revealed more significant collinearity between C. salicifolius and the magnoliids species compared to eudicots and monocots. MIKC-type MADS-box genes from the same subfamily share similar distribution patterns, gene structure, and expression patterns. Compared with Arabidopsis thaliana, Nymphaea colorata, and Chimonanthus praecox, the FLC genes were absent in C. salicifolius, while the AGL6 subfamily was expanded in C. salicifolius. The selectively expanded promoter (AGL6) and lack of repressor (FLC) genes may explain the earlier flowering in C. salicifolius. The loss of the AP3 homologous gene in C. salicifolius is probably the primary cause of the morphological distinction between C. salicifolius and C. praecox. The csAGL6a gene is specifically expressed in the flowering process and indicates the potential function of promoting flowering. Conclusion This study offers a genome-wide identification and expression profiling of the MIKC-types MADS-box genes in the C. salicifolius, and establishes the foundation for screening flowering development genes and understanding the potential function of the MIKC-types MADS-box genes in the C. salicifolius.
Fang, Zhao,Tang, Rui-Ren,Zhang, Rui-Rong,Huang, Ke-long Korean Chemical Society 2009 Bulletin of the Korean Chemical Society Vol.30 No.10
Oxidation of cycloalkenes with $O_2$ promoted by heterogeneous bis(acetylacetonato) cobalt (II) complex catalyst which can be recycled has been performed under mild conditions. It was found that $\beta$-ionone, cyclohexene, 1-methylcyclohexene, and $\alpha$-ionone were efficiently oxidized with $O_2$ in the presence of Co (II) complex and butyraldehyde at $55\;{^{\circ}C}$. A simple treatment of the resulting products led to epoxides as predominant products and a small amounts of allylic oxides, the chemoselectivity for the former being 82.1 - 90.8% with a 70.6 - 98.6% substrate conversion. On the other hand, oxidation of 1-phenylcyclohexene, 1-cyclohex-1-enylethan-1-one, $\alpha$-pinene, and $\beta$-pinene gave allylic oxides as major products.
Saturation impulses for dynamically loaded structures with finite-deflections
Zhao, Ya-Pu,Yu, T.X.,Fang, J. Techno-Press 1995 Structural Engineering and Mechanics, An Int'l Jou Vol.3 No.6
The concept of "Saturation Impulse" for rigid, perfectly plastic structures with finite-deflections subjected to dynamic loading was put forward by Zhao, Yu and Fang (1994a). This paper extends the concept of Saturation Impulse to the analysis of structures such as simply supported circular plates, simply supported and fully clamped square plates, and cylindrical shells subjected to rectangular pressure pulses in the medium load range. Both upper and lower bounds of nondimensional saturation impulses are presented.
Fang Zhao,Yu Qi,Wei Xiong 대한화학회 2012 Bulletin of the Korean Chemical Society Vol.33 No.1
A novel chemiluminescence (CL) flow injection method for the determination of balofloxacin is described. The method is based on the weak CL signal arising from the reaction of KBrO3 with Na2S2O4 in acidic medium being significantly enhanced by balofloxacin in the presence of europium (III) ion and sodium dodecyl benzene sulfonate (SDBS). The experimental conditions that affected CL intensity were carefully optimized and the CL reaction mechanism was briefly discussed. Under the optimum conditions, the relative CL intensity was proportional to the concentration of balofloxacin in the range of 7.0 × 10−11 to 3.0 × 10−7 g mL−1. The detection limit was 2.7 × 10−11 g mL−1 and the relative standard deviation was 2.1% for 7.0 × 10−10 g mL−1 balofloxacin (n = 13). The proposed method was successfully applied to the determination of balofloxacin in pharmaceutical formulations and biological fluids.
Fang, Minfeng,Wang, Hui,Wu, Yang,Wang, Qilin,Zhao, Xinfeng,Zheng, Xiaohui,Wang, Shixiang,Zhao, Guifang Korean Chemical Society 2013 Bulletin of the Korean Chemical Society Vol.34 No.6
A rapid and sensitive method for determining usnic acid of Lethariella cladonioides in rat was established using high performance liquid chromatography (HPLC) quadrupole time-of-flight (QTOF) tandem mass (MS/MS). Rat plasma was pretreated by mixture of acetonitrile and chloroform to precipitate plasma proteins. Chromatographic separation was achieved on a column ($50{\times}2.1$ mm, $5{\mu}m$) with a mobile phase consisting of water (containing $5{\times}10^{-3}$ M ammonium formate, pH was adjusted to 3.0 with formic acid) and acetonitrile (20:80, v/v) at a flow rate of 0.3 mL/min. A tandem mass spectrometric detection with an electrospray ionization (ESI) interface was conducted via collision induced dissociation (CID) under negative ionization mode. The MS/MS transitions monitored were m/z 343.0448 ${\rightarrow}$ m/z 313.2017 for usnic acid and m/z 153.1024 ${\rightarrow}$ m/z 136.2136 for protocatechuic acid (internal standard). The linear range was calculated to be 2.0-160.0 ng/mL with a detection limit of 3.0 pg/mL. The inter- and intra-day accuracy and precision were within ${\pm}7.0%$. Pharmacokinetic study showed that the apartment of usnic acid in vivo confirmed to be a two compartment open model. The method was fully valid and will probably be an alternative for pharmacokinetic study of usnic acid.
RAMS evaluation for a steel-truss arch high-speed railway bridge based on SHM system
Zhao, Han-Wei,Ding, You-Liang,Geng, Fang-Fang,Li, Ai-Qun Techno-Press 2018 Structural monitoring and maintenance Vol.5 No.1
The evaluation theory of reliability, availability, maintainability and safety (RAMS) as a mature theory of state evaluation in the railway engineering, can be well used to the evaluation, management, and maintenance of complicated structure like the long-span bridge structures on the high-speed railway. Taking a typical steel-truss arch bridge on the Beijing-Shanghai high-speed railway, the Nanjing Dashengguan Yangtze River Bridge, this paper developed a new method of state evaluation for the existing steel-truss arch high-speed railway bridge. The evaluation framework of serving state for the bridge structure is presented based on the RAMS theory. According to the failure-risk, safety/availability, maintenance of bridge members, the state evaluation method of each monitoring item is presented. The weights of the performance items and the monitoring items in all evaluation levels are obtained using the analytic hierarchy process. Finally, the comprehensive serving state of bridge structure is hierarchical evaluated.