UV-grafting Coloration of Cotton and Wool Fabrics Using Bismethacrylated Quinizarin Dye

Dong, Yuanyuan,Zheng, Laijiu,Wei, Ju,Jang, Jinho 한국섬유공학회 2016 Fibers and polymers Vol.17 No.10

A novel bifunctional quinizarin dye possessing two photoactive methacrylate groups was synthesized by the reaction of quinizarin with methacryloyl chloride. The synthesized dye, a low substantive dye under the conventional dyeing process, can be photografted onto cotton and wool fabrics at room temperature without neutral salts, which makes it a novel coloration process of excellent environmental friendliness. The concurrent polymerization and grafting of the synthesized dye onto cotton or wool can be assisted by a photoinitiator and acrylic acid in the case of cotton grafting. Moreover, color yields of the grafted fabrics improved significantly with the photografting of the bifunctional dye. The bifunctional dye can be photopolymerized with the increase in UV energy to <TEX>$25J/cm^2$</TEX> and the oligomeric dye has a degree of polymerization of 5 or more. Furthermore, the color fastness properties of the grafted fabrics were superior to those of the dyed fabrics via exhaustion.

The Effect of Inhibition of Uncaria rhynchophylla as an Inhibitor of Melanogenesis and an Antioxidant in B16F10 Melanoma Cells

Yuanyuan Dong(동원원),Young Min Woo(우영민),Ji Hyun Cha(차지현),Jae Young Cha(차재영),Nai Wei Lee(려내유),Min Woo Back(백민우),Joon-sung Park(박준성),Sang-Hyeon Lee(이상현),Jong-Myung Ha(하종명),Andre Kim(김안드레) 한국생명과학회 2020 생명과학회지 Vol.30 No.12

최근 모든 연령대의 사람들은 미용적인 이유로 더 밝은 피부를 원하고 있으며, 천연 제품은 화학적으로 합성된 화합물보다 더 많은 관심을 받고 있다. 조구등은 아시아에서 전통 한약재로 널리 사용되어 왔다. 새로운 피부미백제를 찾기 위해 본 연구에서는 조구등의 항산화 활성과 잠재적인 tyrosinase 억제 작용을 확인하였다. 조구등을 70% 에탄올로 추출하여 항산화 활성을 분석하고 tyrosinase 활성과 melanin 합성에 미치는 영향을 평가했다. 총 mRNA 발현은 RT-PCR을 사용하였다. 그 결과 조구등 추출물은 B16F10 세포에서 뛰어난 항산화 능력과 상당한 수준의 폴리페놀 및 플라보노이드 화합물을 함유하였다. 또한, 세포 내 tyrosinase 활성을 억제하고 처리된 세포에서 melanin의 양을 감소시켰다. Tyrosinase의 mRNA 발현은 1 mg/ml 농도에서 현저히 감소하였다. 이와 같은 결과는 조구등이 미백 효과가 있는 화장품의 천연 소재로 사용될 수 있는 높은 잠재력을 가지고 있음을 시사한다. Many people of all ages wish to have lighter skin for cosmetic reasons, and natural products attract more attention than chemically synthesized compounds. Uncaria rhynchophylla is widely used in Asia as a traditional herbal medicine. In order to find novel skin whitening agents, the present study evaluated the antioxidant activity and potential tyrosinase-inhibiting properties of U. rhynchophylla. Specifically, this study analyzed the antioxidant capacity of a 70% ethanolic extract of U. rhynchophylla as well as its effects on tyrosinase activity and melanin synthesis. Total mRNA levels were examined using reverse transcription polymerase chain reaction. The results revealed that U. rhynchophylla extracts exhibit great antioxidant capacity and significant levels of polyphenol and flavonoid compounds. U. rhynchophylla extracts can also powerfully inhibit tyrosinase activity. This same capacity was observed in melanoma B16F10 cells; that is, U. rhynchophylla extracts suppressed intracellular tyrosinase activity and reduced the amount of melanin in treated cells. In addition, a 1 mg/ml concentration of U. rhynchophylla extract significantly reduced the mRNA expression levels of tyrosinase. U. rhynchophylla extracts decrease tyrosinase and inhibit melanogenesis in B16F10 cells. This finding suggests that U. rhynchophylla has great potential as a natural whitening agent in skincare products.

Union Dyeing of the Photografted PET/Wool Blend Fabrics with Dimethylaminopropyl Methacrylamide

Dong, Yuanyuan,Lyoo, Won-Seok,Jang, Jin-Ho 한국섬유공학회 2010 FIBERS AND POLYMERS Vol.11 No.2

Dimethylaminopropyl methacrylamide (DMAPMA) was grafted onto PET/wool blend fabrics by continuous UV irradiation. Union dyeing of the photografted fabrics was investigated using three reactive dyes of ${\alpha}$-bromoacrylamide reactive groups. The influence of grafting yield, DMAPMA concentration, NaCl amount, pH value, and dyeing temperature on the dyeability was evaluated. The dyeability of both PET and wool components was improved significantly by the DMAPMA photografting and successive reactive dyeing. Although the dyeability of the PET component in the blend substantially was improved with higher grafting, equal dyeability between PET and wool was difficult to achieve due to more facile grafting and higher reactivity of the wool component compared with the modified PET component. However, the color fastness of the PET/wool blend fabric was excellent for all three colors. This study may offer a way to achieve union dyeing of PET/wool blend fabrics.

Photoinitiator-free Photo-reactive Coloration of Wool Fabrics Using C.I. Reactive Black 5

Yuanyuan Dong,Jinho Jang 한국염색가공학회 2012 韓國染色加工學會誌 Vol.24 No.2

Novel Coloration of Cotton Fabrics by UV-induced Phtografting of Reactive Black 5 and Acrylic acid

Yuanyuan Dong,Jinho Jang 한국염색가공학회 2011 韓國染色加工學會誌 Vol.23 No.1

Developmental Exposure to Di-(2-ethylhexyl) Phthalate Induces Cerebellar Granule Cell Apoptosis via the PI3K/AKT Signaling Pathway

Yuanyuan Fu,Jing Dong,Jianan Wang,Mingdan You,Lingling Wei,Hui Fu,Yuan Wang,Jie Chen 한국뇌신경과학회 2018 Experimental Neurobiology Vol.27 No.6

Di-(2-ethylhexyl) phthalate (DEHP) is an ubiquitous environmental contaminant because of its extensive use in plastics and its persistence. As an environmental endocrine disruptor, it is suspected to interfere with neurodevelopment in people. However, evidence of the effects of maternal DEHP exposure on cerebellar development in offspring is scarce. The objective of this study was to investigate maternal exposure to DEHP and its effect on apoptosis of cerebellar granule cells (CGCs) and related mechanisms. Pregnant Wistar rats were administrated DEHP (0, 30, 300 and 750 mg/kg/d) by gavage from gestational day (GD) 0 to postnatal day (PN) 21. Primary CGCs were also exposed to mono-(2-ethylhexyl) phthalate (MEHP), the main metabolite of DEHP, for 24 h with concentrations of 0, 25, 100 and 250 μM. The CGCs of male offspring from 300 and 750 mg/kg/d DEHP exposure groups showed significantly increased apoptosis. In addition, the PI3K/AKT signaling pathway was inhibited in the male offspring of the 300 and 750 mg/ kg/d DEHP exposure groups. However, effects on female pups were not obvious. Apoptosis was also elevated and the PI3K/AKT signaling pathway was inhibited after primary CGCs were exposed to MEHP. Furthermore, apoptosis was reduced after treatment with the PI3K/AKT signaling pathway activator, insulin-like growth factor (IGF) 1, and increased after treatment with LY294002, an inhibitor of the PI3K/AKT signaling pathway. These results suggested that maternal DEHP exposure induced apoptosis in the CGCs of male pups via the PI3K/AKT signaling pathway, and the apoptosis could be rescued by IGF1 and aggravated by LY294002.

Exploratory research on ultra-long polymer optical fiber-based corrosion sensing for buried metal pipelines

Dong Luo,Yuanyuan Li,Hangzhou Yang,Hao Sun,Hongbin Chen 국제구조공학회 2020 Smart Structures and Systems, An International Jou Vol.26 No.4

In order to achieve effective corrosion monitoring of buried metal pipelines, a Novel nondestructive Testing (NDT) methodology using ultra-long (250 mm) Polymer Optical Fiber (POF) sensors coated with the Fe-C alloy film is proposed in this study. The theoretical principle is investigated to clarify the monitoring mechanism of this method, and the detailed fabrication process of this novel POF sensor is presented. To validate the feasibility of this novel POF sensor, exploratory research of the proposed method was performed using simulated corrosion tests. For simplicity, the geometric shape of the buried pipeline was simulated as a round hot-rolled plain steel bar. A thin nickel layer was applied as the inner plated layer, and the Fe-C alloy film was coated using an electroless plating technique to precisely control the thickness of the alloy film. In the end, systematic sensitivity analysis on corrosion severity was further performed with experimental studies on three sensors fabricated with different metal layer thicknesses of 25 μm, 30 μm and 35 μm. The experimental observation demonstrated that the sensor coated with 25 μm Fe-C alloy film presented the highest effectiveness with the corrosion sensitivity of 0.3364 mV/g at Δm = 9.32 × 10^-4 g in Stage I and 0.0121 mV/g in Stage III. The research findings indicate that the detection accuracy of the novel POF sensor proposed in this study is satisfying. Moreover, the simple fabrication of the high-sensitivity sensor makes it cost-effective and suitable for the on-site corrosion monitoring of buried metal pipelines.

Association of multiple anthropometric indices with in 944,760 elderly Chinese people

Lirong Dong,Yuanyuan Wang,Jinshui Xu,Yang Zhou,Guiju Sun,Dakang Ji,Haijian Guo,Baoli Zhu 한국역학회 2023 Epidemiology and Health Vol.45 No.-

OBJECTIVES: The aims of this study were to update the latest data on the prevalence of hypertension (HTN) in the elderly Chinese population and to assess relationships between new anthropometric indices and HTN. METHODS: Data were obtained from the Basic Public Health Service (BPHS) survey for Jiangsu Province, China. A total of 944,760 people aged 65 years and older were included in this study. Blood pressure was measured by trained investigators. Body weight, body mass index (BMI), waist circumference (WC), waist-to-height ratio (WtHR), conicity index (COI), body roundness index (BRI), and a body shape index (ABSI) were included in the analysis as anthropometric indices. Logistic regression analysis and restricted cubic splines were used to evaluate the association of anthropometric indices with HTN. RESULTS: The prevalence of HTN among elderly residents of Jiangsu Province was 64.7% (95% confidence interval, 64.6 to 64.8). After adjusting for multiple covariates, all anthropometric indices except ABSI showed significant non-linear positive dose-response associations with HTN across sex (pnonlinear<0.001). Among participants with BMI <28 kg/m2, abnormal weight, WC, WtHR, BRI, COI, and ABSI were positively associated with HTN. CONCLUSIONS: The prevalence of HTN in the elderly in Jiangsu Province is gradually increasing. It is necessary to consider the combination of ABSI and COI with BMI for screening elderly individuals for HTN in follow-up prospective studies.

Ginsenosides Rc, as a novel SIRT6 activator, protects mice against high fat diet induced NAFLD

Zehong Yang,Yuanyuan Yu,Nannan Sun,Limian Zhou,Dong Zhang,HaiXin Chen,Wei Miao,Weihang Gao,Canyang Zhang,Changhui Liu,Xiaoying Yang,Xiaojie Wu,Yong Gao The Korean Society of Ginseng 2023 Journal of Ginseng Research Vol.47 No.3

Background: Hepatic lipid disorder impaired mitochondrial homeostasis and intracellular redox balance, triggering development of non-alcohol fatty liver disease (NAFLD), while effective therapeutic approach remains inadequate. Ginsenosides Rc has been reported to maintain glucose balance in adipose tissue, while its role in regulating lipid metabolism remain vacant. Thus, we investigated the function and mechanism of ginsenosides Rc in defending high fat diet (HFD)-induced NAFLD. Methods: Mice primary hepatocytes (MPHs) challenged with oleic acid & palmitic acid were used to test the effects of ginsenosides Rc on intracellular lipid metabolism. RNAseq and molecular docking study were performed to explore potential targets of ginsenosides Rc in defending lipid deposition. Wild type and liver specific sirtuin 6 (SIRT6, 50721) deficient mice on HFD for 12 weeks were subjected to different dose of ginsenosides Rc to determine the function and detailed mechanism in vivo. Results: We identified ginsenosides Rc as a novel SIRT6 activator via increasing its expression and deacetylase activity. Ginsenosides Rc defends OA&PA-induced lipid deposition in MPHs and protects mice against HFD-induced metabolic disorder in dosage dependent manner. Ginsenosides Rc (20mg/kg) injection improved glucose intolerance, insulin resistance, oxidative stress and inflammation response in HFD mice. Ginsenosides Rc treatment accelerates peroxisome proliferator activated receptor alpha (PPAR-α, 19013)-mediated fatty acid oxidation in vivo and in vitro. Hepatic specific SIRT6 deletion abolished ginsenoside Rc-derived protective effects against HFD-induced NAFLD. Conclusion: Ginsenosides Rc protects mice against HFD-induced hepatosteatosis by improving PPAR-α-mediated fatty acid oxidation and antioxidant capacity in a SIRT6 dependent manner, and providing a promising strategy for NAFLD.

Cytosolic 5′-Methylthioadenosine/ S-Adenosylhomocysteine Nucleosidase MtaN from Mycobacterium. smegmatis: purification, crystallization and X-ray crystallographic analysis

Lulu Wang,Yuanyuan Chen,Wei Liu,Jing Lan,Fei Shang,Nam-Chul Ha,Yuesheng Dong,Chunshan Quan,Yongbin Xu 한국구조생물학회 2019 Biodesign Vol.7 No.3

Mycobacterium tuberculosis is a dangerous pathogen, and it can cause the most deadly disease tuberculosis (TB). Nonpathogenic Mycobacterium smegmatis is an important model for studying the M. tuberculosis. M. smegmatis 5’-Methylthioadenosine/S-adenosyl-L-homocysteine nucleosidases (MtaNs) catalyze the hydrolysis of adenine from 5’-methylthioadenosine (MTA), MtaNs cleave the glycosidic bond of MTA or S-adenosylhomocysteine (SAH) irreversibly. In this study, MtaN from M. smegmatis (MsMtaN) was successfully expressed and purified using Ni-NTA affinity, Q anionexchange and gel-filtration chromatography. The protein crystal was obtained and diffracted to a resolution of 2.0 Å. The crystal belonged to the orthorhombic space group P1211, with unit-cell parameters of a =57.6, b = 172.6, and c = 183.3 Å. The Matthews coefficient and solvent content were estimated to be 2.32 Å3 Da-1 and 47%, respectively, assuming that the asymmetric unit contained only one recombinant protein molecule. Size-exclusion chromatography suggested that MsMtaN prefer to exist as tetramer in solution.