http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
Comprehensive analysis of CCCH zinc-finger-type transcription factors in the Brassica rapa genome
Jana Jeevan Rameneni,Vignesh Dhandapani,Parameswari Paul,Sangeeth Prasath Devaraj,최수련,이소영,김만선,홍성민,오상헌,오만호,임용표 한국원예학회 2018 Horticulture, Environment, and Biotechnology Vol.59 No.5
The CCCH-type zinc finger proteins are characterized by their signature motif of three cysteine and one histidine residues. These proteins are RNA binding proteins that function in plant growth, developmental processes, and responses to various environmental stress conditions. In this study, a comprehensive analysis using computational methods allowed the identification of 63 functionally important CCCH genes in the Brassica rapa genome. Several analyses were carried out on the identified genes to understand their roles in this plant. Comparative phylogenetic analysis classified CCCH genes into six clusters, while motif and structural analyses revealed four unique CCCH motifs including different functional motifs and intron/exon variations unique to plants compared to those in other species. Real-time qRT-PCR analysis of 10 randomly selected genes indicated they function at the early stages rather than the later stages of cold and salt stress. This study provides a basic understanding of potential candidate CCCH genes in B. rapa, their structural variation, expression patterns, and their roles under different stress conditions.
Kim, Man-Sun,Hong, Seongmin,Devaraj, Sangeeth Prasath,Im, Subin,Kim, Jeong-Rae,Lim, Yong Pyo Elsevier 2017 Biochemical and biophysical research communication Vol. No.
<P><B>Abstract</B></P> <P>Inner and rosette leaves of Chinese cabbage (<I>Brassica rapa</I>) have different characteristics in terms of nutritional value, appearance, taste, color and texture. Many researchers have utilized differentially expressed genes for exploring the difference between inner and rosette leaves of <I>Brassica rapa</I>. The functional characteristics of a gene, however, is determined by complex interactions between genes. Hence, a noble network approach is required for elucidating such functional difference that is not captured by gene expression profiles alone. In this study, we measured gene expression in the standard cabbage genome by RNA-Sequencing and constructed rosette and inner leaf networks based on the gene expression profiles. Furthermore, we compared the topological and functional characteristics of these networks. We found significant functional difference between the rosette and inner leaf networks. Specifically, we found that the genes in the rosette leaf network were associated with homeostasis and response to external stimuli whereas the genes in the inner leaf network were mainly related to the glutamine biosynthesis processes and developmental processes with hormones. Overall, the network approach provides an insight into the functional difference of the two leaves.</P> <P><B>Highlights</B></P> <P> <UL> <LI> We measured gene expression in the standard cabbage genome by RNA-Sequencing. </LI> <LI> We identified the rosette leaf network and inner leaf network based on the gene expression profiles. </LI> <LI> We compared the topological and functional characteristics of these two networks. </LI> <LI> The genes in the rosette leaf network were mainly associated with response to external stimuli. </LI> <LI> The genes in the inner leaf network were mainly related to developmental processes with hormones. </LI> </UL> </P>