Hepatoprotective Activity of Ethanolic Extract of Bacopa monnieri Linn. AerialParts Against CCl4-induced Hepatotoxicity in Rats

Tirtha Ghosh,Tapan Kumar Maity,Gouri Kumar Dash,Anindya Bose,Siva Sankar Nayak,Deepak Kumar Dash 한국생약학회 2007 Natural Product Sciences Vol.13 No.1

ethanolic extract of Bacopa monnieri Linn. aerial parts were studied for its hepatoprotective efecton CCl4-induced hepatotoxic rats. The extract was found to decrease significantly CCl4-induced elevation ofSGOT, SGPT, ALP, bilirubin and total cholesterol. But, it increased HDL-cholesterol level and liver weight withrespect to CCl4 toxic rats. The extract was also found to decrease significantly the CCl4-induced elevation of lipidperoxidation and increase the activity of antioxidant enzymes (SOD and CAT) and GSH level in the liver ofextract treated rats when compared with CCl4 induced rats. Histopathological profiles showed that the extract hadsignificant protective effect against CCl4may be possible that the mechanism of hepatoprotection of the extract is due to its antioxidant effect.KeywordsBacopa monnieri L., ethanolic extract, hepatoprotective activity, antioxidant activity, histopathology.

Effect of various fractions of Bacopa monnieri Linn. aerial parts on ethanolinduced hepatotoxicity in rats

Tirtha Ghosh,Tapan Kumar Maity,Deepak Kumar Dash,Anindya Bose 경희대학교 융합한의과학연구소 2007 Oriental Pharmacy and Experimental Medicine Vol.7 No.3

The ethyl acetate fraction (EAF) and n-butanol fraction (NBF) of ethanolic extract of Bacopa monnieri aerial parts were screened for hepatoprotective activity and in vivo antioxidant activity on ethanolinduced hepatotoxic rats. Ethyl acetate fraction was found to be more potent even though both the fractions were endowed with significant hepatoprotective activity. EAF and NBF were investigated for hepatoprotective activity in albino rats at 300 mg/kg, p.o. dose and compared with standard drug Silymarin (25 mg/kg, p.o.). Results show that both the fractions were effective in blunting ethanolinduced enhanced activities of serum glutamate oxaloacetate transaminase, serum glutamate pyruvate transaminase, alkaline phosphatase, level of serum bilirubin (both total and direct), liver weight loss and was also effective in reducing ethanol–induced lipid peroxidation both in vitro and in vivo. Furthermore, the fractions could also enhance ethanol-induced suppressed activities of superoxide dismutase, catalase and decreased level of reduced glutathione. Results of hepatocellular damage caused by ethanol and its recovery by EAF and NBF, suggest that they might be considered as a potential source of natural hepatoprotective agents, which could be related to the free radical scavenging properties of saponins present in high concentration in the fractions.

Effect of various fractions of Bacopa monnieri Linn. aerial parts on ethanol-induced hepatotoxicity in rats

Ghosh, Tirtha,Maity, Tapan Kumar,Dash, Deepak Kumar,Bose, Anindya Kyung Hee Oriental Medicine Research Center 2007 Oriental pharmacy and experimental medicine Vol.7 No.3

The ethyl acetate fraction (EAF) and n-butanol fraction (NBF) of ethanolic extract of Bacopa monnieri aerial parts were screened for hepatoprotective activity and in vivo antioxidant activity on ethanol-induced hepatotoxic rats. Ethyl acetate fraction was found to be more potent even though both the fractions were endowed with significant hepatoprotective activity. EAF and NBF were investigated for hepatoprotective activity in albino rats at 300 mg/kg, p.o. dose and compared with standard drug Silymarin (25 mg/kg, p.o.). Results show that both the fractions were effective in blunting ethanol-induced enhanced activities of serum glutamate oxaloacetate transaminase, serum glutamate pyruvate transaminase, alkaline phosphatase, level of serum bilirubin (both total and direct), liver weight loss and was also effective in reducing ethanol-induced lipid peroxidation both in vitro and in vivo. Furthermore, the fractions could also enhance ethanol-induced suppressed activities of superoxide dismutase, catalase and decreased level of reduced glutathione. Results of hepatocellular damage caused by ethanol and its recovery by EAF and NBF, suggest that they might be considered as a potential source of natural hepatoprotective agents, which could be related to the free radical scavenging properties of saponins present in high concentration in the fractions.

A study on wound healing activity of Bacopa monnieri Linn. aerial parts

Ghosh, Tirtha,Maity, Tapan Kumar,Dash, Deepak Kumar,Boss, Anindya Kyung Hee Oriental Medicine Research Center 2007 Oriental pharmacy and experimental medicine Vol.7 No.2

Bacopa monnieri is being used in the traditional system of medicine for a variety of ailments. In the present study, the ethanolic extract of Bacopa monnieri aerial parts has been studied for its wound healing activity using various models in rats. Significant increase in wound contraction and skin breaking strength were observed in the excision and incision wound models respectively. There was also significant increase in hydroxyproline content, DNA content, superoxide dismutase activity, catalase activity, glutathione level and decrease in the level of thiobarbituric acid reactive substances in the $12^{th}$ day post wounding tissue of experimental rats in the ethanol extract treated groups with respect to the control group. The effect of the extract was found to be comparable with the standard drug nitrofurazone. From the results it may be concluded that the plant Bacopa monnieri is endowed with significant wound healing activity, thereby justifying its use in the traditional medicine. Saponins may be responsible for the observed wound healing activity.

A study on wound healing activity of Bacopa monnieri Linn. aerial parts

Tirtha Ghosh,Tapan Kumar Maity,Deepak Kumar Dash,Anindya Bose 경희대학교 융합한의과학연구소 2007 Oriental Pharmacy and Experimental Medicine Vol.7 No.2

Bacopa monnieri is being used in the traditional system of medicine for a variety of ailments. In the present study, the ethanolic extract of Bacopa monnieri aerial parts has been studied for its wound healing activity using various models in rats. Significant increase in wound contraction and skin breaking strength were observed in the excision and incision wound models respectively. There was also significant increase in hydroxyproline content, DNA content, superoxide dismutase activity, catalase activity, glutathione level and decrease in the level of thiobarbituric acid reactive substances in the 12th day post wounding tissue of experimental rats in the ethanol extract treated groups with respect to the control group. The effect of the extract was found to be comparable with the standard drug nitrofurazone. From the results it may be concluded that the plant Bacopa monnieri is endowed with significant wound healing activity, thereby justifying its use in the traditional medicine. Saponins may be responsible for the observed wound healing activity.

Evaluation of Antidiabetic and Antihyperlipidemic Activity of Luffa tuberosa(Roxb.) Fruits in Streptozotocin Induced Diabetic Rats

Veerendra C. Yeligar,K. Murugesh,Deepak Kumar Dash,Siva Shankar Nayak,B. C. Maiti,Tapan K. Maity 한국생약학회 2007 Natural Product Sciences Vol.13 No.1

unripe whole fruits of Luffa tuberosa (Roxb.) or Momordica tuberosa (Roxb) Cogn.(Cucurbitaceae) were evaluated for the antidiabetic and hyperlipidemic potential in streptozotocin (STZ) induceddiabetic rats. Diabetes was induced by administration of intra-peritoneal injection of streptozotocin at a dose of55 mg/kg body weight. After the induction of diabetes aqueous extract of L.tuberosa (AELT) was administeredorally at doses of 250 and 500 mg/kg. body weight/day for a period of 14 days. The Fasting blood glucose (FBG)levels, serum insulin levels, changes in body weight, food and liquid intake were measured. In diabetic rats, theAELT exhibited significant reduction in blood glucose levels. Biochemical assay of plasma total cholesterol (TC),triglyceride (TG), high-density lipoprotein cholesterol (HDLc), low-density lipoprotein cholesterol (LDLc),after the extract supplementation was confirmed by significant reduction in the levels of above mentionedhyperlipidemic indicators. This study focus on the efficacy of L.tuberosa fruits for the management ofexperimental STZ induced diabetic rats and provides the scientific basis of ancient herbal therapy and use of thesefruits as vegetable.KeywordsLuffa tuberosa (Roxb.), streptozotocin, antihyperglycemic activity, antihyperlipidemic activity

Effect of Berberis tinctoria leaf (Berberidaceae) extract on antidiabetic, antihyperlipidemic and antioxidant status in streptozotocin induced diabetes in rats

Murugesh, K,Yeligar, Veerendra C,Dash, Deepak Kumar,Maiti, BC,Maity, Tapan K Kyung Hee Oriental Medicine Research Center 2006 Oriental pharmacy and experimental medicine Vol.6 No.4

The present study was carried out to investigate the antidiabetic and antioxidant effect of methanolic extract of Berberis tinctoria leaves (MEBT), in streptozotocin induced diabetic rats. Oral administration of MEBT extract (150 mg/kg and 300 mg/kg) for a period of 14 days. Blood glucose levels, body weight food and liquid intake were measured on every $5^{th}$ day over a period of 14 days. In diabetic rats, MEBT at the dose of 150 mg/kg and 300 mg/kg body weight resulted in significant reduction in blood glucose levels. The study was further investigated to determine antioxidant and antihyperlipidemic potential of MEBT in streptozotocin (STZ) induced diabetic rats. These results suggest that the MEBT possess antidiabetic activity and is able to ameliorate biochemical damages in STZ induced diabetic rats and the results were found to be in a dose dependent manner.