Fatigue performance of deepwater SCR under short-term VIV considering various S-N curves

Kim, D.K.,Choi, H.S.,Shin, C.S.,Liew, M.S.,Yu, S.Y.,Park, K.S. Techno-Press 2015 Structural Engineering and Mechanics, An Int'l Jou Vol.53 No.5

In this study, a method for fatigue performance estimation of deepwater steel catenary riser (SCR) under short-term vortex-induced vibration was investigated for selected S-N curves. General tendency between S-N curve capacity and fatigue performance was analysed. SCRs are generally used to transport produced oil and gas or to export separated oil and gas, and are exposed to various environmental loads in terms of current, wave, wind and others. Current is closely related with VIV and it affects fatigue life of riser structures significantly. In this regards, the process of appropriate S-N curve selection was performed in the initial design stage based on the scale of fabrication-related initial imperfections such as welding, hot spot, crack, stress concentration factor, and others. To draw the general tendency, the effects of stress concentration factor (SCF), S-N curve type, current profile, and three different sizes of SCRs were considered, and the relationship between S-N curve capacity and short-term VIV fatigue performance of SCR was derived. In case of S-N curve selection, DNV (2012) guideline was adopted and four different current profiles of the Gulf of Mexico (normal condition and Hurricane condition) and Brazil (Amazon basin and Campos basin) were considered. The obtained results will be useful to select the S-N curve for deepwater SCRs and also to understand the relationship between S-N curve capacity and short-term VIV fatigue performance of deepwater SCRs.

Comparison of 90‐day case‐fatality after ischemic stroke between two different stroke outcome registries using propensity score matching analysis

Yu, K‐,H.,Hong, K‐,S.,Lee, B‐,C.,Oh, M‐,S.,Cho, Y‐,J.,Koo, J‐,S.,Park, J‐,M.,Bae, H‐,J.,Han, M‐,K.,Ju, Y‐,S.,Kang, D‐,W.,Appelros, P. Blackwell Publishing Ltd 2011 Acta neurologica Scandinavica Vol.123 No.5

<P>Yu K‐H, Hong K‐S, Lee B‐C, Oh M‐S, Cho Y‐J, Koo J‐S, Park J‐M, Bae H‐J, Han M‐K, Ju Y‐S, Kang D‐W, Appelros P, Norrving B, Terent A. Comparison of 90‐day case‐fatality after ischemic stroke between two different stroke outcome registries using propensity score matching analysis. 
Acta Neurol Scand: 2011: 123: 325–331. 
© 2010 John Wiley & Sons A/S.</P><P><B>Background – </B> It has not been clarified whether the disparity in ischemic stroke outcome between populations is caused by ethnic and geographic differences or by variations in case mix. Propensity score matching (PSM) analysis can overcome some analytical problems but is rarely used in stroke outcome research. This study was to compare the ischemic stroke case‐fatality between two PSM cohorts of Sweden and Korea.</P><P><B>Methods – </B> Prognostic variables related to baseline characteristics and stroke care were included in our PSM model. Then, we selected 7675 Swedish and 1220 Korean patients with ischemic stroke from each stroke registers and performed one‐to‐one matching based on propensity scores of each patient.</P><P><B>Results – </B> After PSM, all measured variables were well balanced in 1163 matched subjects, and the 90‐day case‐fatality was identical 6.2% (HR 0.997, 95%CI 0.905–1.099) in Sweden and Korea.</P><P><B>Conclusions – </B> No difference is found in the 90‐day case‐fatality in propensity score‐matched Swedish and Korean patients with ischemic stroke.</P>

20대 여성의 데이트폭력 피해 경험과 우울, 불안 및 신체화의 관계

김수민,김예진,김유정,남지현,박금빈,박이준,안혜민,오세연,유가현,유다현,전예지,김옥수,길민지 이화여자대학교간호학회 2020 이화간호학회지 Vol.- No.54

Purpose: The purpose of this study is to examine the degree of date violence damage, depression, anxiety, and somatization among women in their 20s who had experienced date violence, and to investigate the correlations b etween the subjects’ experience of date violence damage and depression, anxiety and somatization. Method: The subject of this study were collected from September 04 to October 13, 2019, by posting advertisements on the women’s university community site in Seoul and a representative women;s community site on the Internet. 149 subjects were collected in their 20s who had experienced date violence from their partners. SPSS statistics 22.0 statistical program was used for data analysis, and standard deviation, T-test, ANOVA, and Pearson’s correlation coefficients were performed. Result: The results showed that the degree of experience of date violence was significantly correlated with symptoms of depression, anxiety, and somatization. Conclusion: Since there was correlations b etween depression, anxiety, and somatization symptoms in date violence victims, it is necessary to apply nursing intervention related to depression and anxiety to prevent somatization symptoms caused by date violence.

도금슬러지 시멘트고형화에서의 강도 및 안정화 증진

윤현식,강민성,이두진,신응배,배우근,유도윤 漢陽大學校 環境工學硏究所 1997 環境科學論文集 Vol.18 No.-

본 연구에서는 도금폐수처리공정에서 발생하는 실 슬러지를 대상으로 현장에서 적용이 가능한 시멘트고형화 및 안정화효율 증진방안을 찾기 위하여 실내 실험을 통해 1) 슬러지내의 수분이 시멘트의 수화반응에 미치는 영향, 2) 교반시간증가에 의한 고화체의 강도향상 및 용출억제효과, 그리고 3) 첨가제를 이용한 고형화효율 증대를 중점적으로 살펴 보았다. 실슬러지의(습윤슬러지) 자체가 함유한 수분이 시멘트수화반응에 미치는 영향을 살펴보기 위하여 슬러지를 건조시킨 후 물을 첨가하여 시멘트모르터내에 자유수를 증가시킨 결과 습윤슬러지를 그대로 고형화 한 경우보다 동일한 수분에서 2배정도의 높은강도를 얻을 수 있었다. 교반시간을 증가시킬수록 강도와 용출성이 점차 향상되어 10분정도의 교반시간에서 우수한 결과를 얻었으며, 특히 습윤슬러지의 경우 그 효과가 두드러지게 나타났다. 첨가제사용에 따른 효율평가 실험은 Silica fume, 황산반토, Zeolite, EDTA를 대상으로 실시하였으며 이중 Silica fume이 강도와 용출억제면에서 가장 효과가 좋았다. 최적배합비는 습윤슬러지의 경우 첨가비(첨가제/시멘트)0.25∼0.5에서 가장 우수하였고, 0.05∼0.1에서 가장 좋은 용출효과를 나타내었다. 이는 Silica fume이 중금속에 의한 시멘트수화 방해작용을 억제하고 중금속이온을 불용성의 화합물로 고정하기 때문으로 사료된다. The Solidification /Stabilization (S/S) using the cement as a binder is widely used because of its stability and low cost. For cement-based S/S, however, the S/S efficiency in terms of strength and contaminant immobilization varies significantly according to the treated waste types and operational conditions. In this research real planting sludges were choosen for the S/S experiments in order to increase the applicability of the results in the actual site. Especially, the study focused on the effects of 1) the moisture of sludge on the hydration-reaction of cements, 2) the increased mixing time on the enhancement of the strength and immobilization, and 3) using the binder additives on the S/S efficiency. The compressive strength and leachability of the heavy metals (CU, Cd, Pb, Cr, and Zn) from the solidified materials were measured for evaluating the S/S efficiency. Drying the sludge and putting water, keeping the moisture content the same as the original sludge, into the cement mortar for supplying free-water increased the compressive strength of the solidified material by appoximately two times. Prolonged mixing of the sludge-cement mixture increased, particually in the wet sludges, the strength of the solidified material and retarded the mobility of heavy metals from the sludge. Good results obtained at ten minutes of mixing. In the experiments using the additives, silica fume gave superior results with the strength and immobilization to other additives (Al₂(SO₄)₃Zeolite, EDTA) tested. In the wet sludges, the optimum additive/cement ratio was 0.25-0.5 for strength and 0.05-0.1 for immobilization. It was suspected that the silica fume prevented the interference of heavy metals on the hydration reaction of cement and fixed the heavy metal ions into insoluble complexes.

Inbred 마우스 수정란을 이용한 형질전환마우스 생산에 있어서의 효과적인 수정란 이식 방법에 관한 연구

Wang A. G,S. U. Kim,Y. H. Han,S. K. Kim,D. Y. Yu 한국동물생명공학회(구 한국동물번식학회) 2003 Reproductive & developmental biology Vol.27 No.1

본 연구의 목적은 inbred 마우스 (C57BL/6J)의 수정란을 이용하여 형질전환마우스를 생산할 때, 수정란이식의 효율성을 증진시키기 위한 것이다. C57BL/6J 및 BCF1 마우스로부터 과배란처리 방법에 의해 수정란을 얻고, DNA를 1 세포기 수정란에 미세 주입한 다음, 1세포기 또는 2 세포기의 수정란을 가임신된 마우스의 한쪽 또는 양쪽 난관에 각각 이식하였다. 1세포기의 수정란을 0.75 d.p.c. 가임신된 마우스의 한쪽 난관에 이식했을 때, 임신율이 C57BL/6J는 68.8±7.83%, BCF1은 48.3±14.22% 이었고, 이식한 수정란 당 산자의 발달율은 C57BL/6J가 11.9±5.51%, BCF1은 10.5±8.03%로 성적이 저조하였다. 그러나, 2세포기의 수정란을 0.5 d.p.c. 가임신된 마우스의 양쪽 난관에 이식했을 때, 임신율이 C57BL/6J는 94.4±9.64%, 13CFl은 100±0% 이었고, 이식한 수정란 당 산자의 발달율은 C57BL/6J가 22.1 ±0.4%, BCF1은 21.8±0.38%였다. 따라서 C57BL/6J 마우스의 2세포기 수정란을 0.5 d.p.c. 가임신된 마우스의 양쪽 난관에 이식하는 것이, BCF1마우스와 유사한 성적을 얻어 경쟁력이 있는 것으로 판단되었다. 이러한 결과에 영향을 미치는 인자가 여러 가지 있을 것으로 판단되지만, C57BL/6J 마우스의 2세포기 수정란을 0.5 d.p.c.가임신된 마우스의 양쪽 난관에 이식하는 방법이 다른 방법보다 형질전환마우스를 생산하는데 효율성이 더 높은 것으로 본 실험에서 확인되었다. The objective of this study is to improve the efficiency of embryo transfer in generation of transgenic mice using inbred mouse (C57BL/6J) embryos. The embryos of C57BL/6J and BCF1 mice were superovulated by the standard protocol. One-cell stage of embryos were microinjected and the resulted one-or two-cell were transferred into one-or two-side oviducts of foster mother, respectively. When one-cell stage of embryos were transferred into one-side oviduct of 0.75 d.p.c. foster mother, the results were not ideal because of showing pregnancy ratios of 68.8±7.83% for C57BL/6J and 48.3±14.22% for BCF1, and development ratios of pups vs transferred embryos of 11.9±5.51% for C57BL/6J and 10.5±8.03% fur BCF1 . However, when two-cell stage of embryos were transferred into two-side oviducts of 0.5 d.p.c. foster mother, we got better results of 94.4±9.64% and 100±0% pregnancy ratio, and 22.1±0.4% and 21.8±0.38% development ratio for C57BL/6J and BCF1, respectively. Therefore, transferring two-cell stage of C57BL/6J embryos into two-side oviducts of 0.5 d.p.c. foster mother may be competitive to the result in BCF1 embryos. Even if there are a lot of other factors affecting these results, we conclude from these experiments that transfer of two-cell embryos into two-side oviducts of 0.5 d.p.c. foster mother is a more efficient and safe method than others in generating transgenic mice using inbred mice embryos.

Peroxiredoxin II promotes hepatic tumorigenesis through cooperation with Ras/Forkhead box M1 signaling pathway

Park, Y-H,Kim, S-U,Kwon, T-H,Kim, J-M,Song, I-S,Shin, H-J,Lee, B-K,Bang, D-H,Lee, S-J,Lee, D-S,Chang, K-T,Kim, B-Y,Yu, D-Y Macmillan Publishers Limited 2016 Oncogene Vol.35 No.27

<P>The current study was carried out to define the involvement of Peroxiredoxin (Prx) II in progression of hepatocellular carcinoma (HCC) and the underlying molecular mechanism(s). Expression and function of Prx II in HCC was determined using H-ras(G12V)-transformed HCC cells (H-ras(G12V)-HCC cells) and the tumor livers from H-ras(G12V)-transgenic (Tg) mice and HCC patients. Prx II was upregulated in H-ras(G12V)-HCC cells and H-ras(G12V)-Tg mouse tumor livers, the expression pattern of which highly similar to that of forkhead Box M1 (FoxM1). Moreover, either knockdown of FoxM1 or site-directed mutagenesis of FoxM1-binding site of Prx II promoter significantly reduced Prx II levels in H-ras(G12V)-HCC cells, indicating FoxM1 as a direct transcription factor of Prx II in HCC. Interestingly, the null mutation of Prx II markedly decreased the number and size of tumors in H-ras(G12V)-Tg livers. Consistent with this, knockdown of Prx II in H-ras(G12V)-HCC cells reduced the expression of cyclin D1, cell proliferation, anchorage-independent growth and tumor formation in athymic nude mice, whereas overexpression of Prx II increased or aggravated the tumor phenotypes. Importantly, the expression of Prx II was correlated with that of FoxM1 in HCC patients. The activation of extracellular signal-related kinase (ERK) pathway and the expression of FoxM1 and cyclin D1 were highly dependent on Prx II in H-ras(G12V)-HCC cells and H-ras(G12V)-Tg livers. Prx II is FoxM1-dependently- expressed antioxidant in HCC and function as an enhancer of Ras(G12V) oncogenic potential in hepatic tumorigenesis through activation of ERK/FoxM1/cyclin D1 cascade.</P>

Evaluation of Pharmacokinetics and Skin Irritation of Transdermal Ketoprofen Plaster(Kefen[R]) in Healthy Volunteers

Yim, D. S 大韓臨床藥理學會 1996 臨床藥理學會誌 Vol.4 No.2

Aqueous and dietary bioaccumulation of antibiotic tetracycline in D. magna and its multigenerational transfer

Kim, H.Y.,Jeon, J.,Hollender, J.,Yu, S.,Kim, S.D. Elsevier Scientific Pub. Co 2014 Journal of hazardous materials Vol.279 No.-

The potential bioaccumulation and distribution of antibiotics in non-target organisms have been inadequately studied in spite of their widespread occurrence in aquatic systems. We investigated the ability of tetracycline to bioaccumulate through aqueous and dietary routes in an aquatic organism, the freshwater crustacean Daphnia magna. D. magna was exposed to algal food (Pseudokirchneriella subcapitata) contaminated with tetracycline for dietary uptake. Tetracycline was transferred to D. magna more through aqueous uptake than through dietary uptake. The uptake rate constant of tetracycline for D. magna was k<SUB>in,water</SUB>=0.33+/-0.045 via the aqueous route and k<SUB>in,food</SUB>=0.16+/-0.012 via the dietary route for 1.0mgL<SUP>-1</SUP> tetracycline. Bioconcentration factors of 4.40+/-0.91Lkg<SUP>-1</SUP> and 3.66+/-0.50Lkg<SUP>-1</SUP> for 0.1 and 1.0mgL<SUP>-1</SUP> tetracycline were found for D. magna. The biomagnification factor of 0.19+/-0.04 indicates that magnification of tetracycline through the food web will not occur. The change in the internal concentration of the target compound was also studied for multigenerational (F1-F4) exposure. The internal concentration in D. magna showed a decreasing trend with increasing generations except for the parent generation. The bioaccumulation tendency showed a biphasic change in multigenerational exposure.

칸디다증의 면역진단 방법 비교 연구(Ⅱ)

김봉수,김현경,이영선,조인선,동미란,유미옥,오현순,김동한,신종희 국립보건연구원 1994 국립보건원보 Vol.31 No.1

Vitamin D-binding protein interacts with Aβ and suppresses Aβ-mediated pathology

Moon, M,Song, H,Hong, H J,Nam, D W,Cha, M-Y,Oh, M S,Yu, J,Ryu, H,Mook-Jung, I Macmillan Publishers Limited 2013 CELL DEATH AND DIFFERENTIATION Vol.20 No.4

The level of vitamin D-binding protein (DBP) is increased in the cerebrospinal fluid of patients with Alzheimer’s disease (AD), suggesting a relationship with its pathogenesis. In this study, we investigated whether and how DBP is related to AD using several different approaches. A pull-down assay and a surface plasmon resonance binding assay indicated direct interactions between purified DBP and amyloid beta (Aβ), which was confirmed in the brain of AD patients and transgenic AD model mice by immunoprecipitation assay and immunohistochemical double-staining method. Moreover, atomic force microscopic examination revealed that DBP reduced Aβ aggregation in vitro. DBP also prevented Aβ-mediated death in cultured mouse hippocampal HT22 cell line. Finally, DBP decreased Aβ-induced synaptic loss in the hippocampus and rescued memory deficits in mice after injection of Aβ into the lateral ventricle. These results provide converging evidence that DBP attenuates the harmful effects of Aβ by a direct interaction, and suggest that DBP is a promising therapeutic agent for the treatment of AD.