Empirical likelihood of conditional quantile difference with left-truncated and dependent data

Kong Cui-Juan,Liang Han-Ying 한국통계학회 2020 Journal of the Korean Statistical Society Vol.49 No.4

We, in this paper, apply the smoothed and maximum empirical likelihood (EL) methods to construct the confdence intervals of the conditional quantile diference with left-truncated data. In particular, we prove the smoothed empirical log-likelihood ratio of the conditional quantile diference is asymptotically chi-squared when the observations with multivariate covariates form a stationary 훼-mixing sequence. At the same time, we establish the asymptotic normality of the maximum EL estimator for the conditional quantile diference. A simulation study is conducted to investigate the fnite sample behavior of the proposed methods and a real data application is provided.

Expression and Clinical Significance of miRNA-34a in Colorectal Cancer

Ma, Zhi-Bin,Kong, Xiao-Lin,Cui, Gang,Ren, Cui-Cui,Zhang, Ying-Jie,Fan, Sheng-Jin,Li, Ying-Hua Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.21

Background: The aim of this study was to investigate differences of miRNA-34a expression in benign and malignant colorectal lesions. Materials and Methods: Samples of cancer, paraneoplastic tissues and polyps were selected and total RNA was extracted by conventional methods for real-time PCR to detect the miRNA-34a expression. In addition, the LOVO colorectal cancer cell line was cultured, treated with the demethylating agent 5-azacytidine and screened for differentially expressed miRNA-34a. Results: After the drug treatment, the miRNA-34a expression of colorectal cancer cell line LOVO was increased and real-time PCR showed that levels of expression in both cell line and colorectal cancer tissues were low, as compared to paraneoplastic tissue (p<0.05). Polyps tissues had significantly higher expression than paraneoplastic and colorectal cancer samples (p<0.05). Conclusions: miRNA-34a-5p may play a role as a tumor suppressor gene in colorectal cancer, with involvement of DNA methylation.

Maternal Gene Transcription in Mouse Oocytes: Genes Implicated in Oocyte Maturation and Fertilization

CUI, Xiang-Shun,LI, Xing-Yu,YIN, Xi-Jun,KONG, IL Keun,KANG, Jason-Jongho,KIM, Nam-Hyung 家畜繁殖硏究所 2007 Journal of Reproduction and Development Vol.53 No.2

<P>Maternal gene expression is an important biological process in oocyte maturation and early cleavage. To gain insights into oocyte maturation and early embryo development, we used microarray analysis to compare the gene expression profiles of germinal vesicle (GV)- and metaphase II (MII)-stage oocytes. The differences in spot intensities were normalized and grouped using the Avadis Prophetic software platform. Of the 12164 genes examined, we found 1682 genes with more highly expression in GV-stage oocytes than in MII-stage oocytes, while 1936 genes were more highly expressed in MII-stage oocytes (P<0.05). The genes were grouped on the basis of the Panther classification system according to their involvement in particular biological processes. The genes that were up-regulated in GV oocytes were more likely to be involved in protein metabolism and modification, the mitotic cell cycle, electron transport, or fertilization or belong to the microtubule/cytoskeletal protein family. The genes specifically upregulated in the MII oocytes were more likely to be involved in DNA replication, amino acid metabolism, or expression of G protein-coupled receptors and signaling molecules. Identification of genes that are preferentially expressed at particular oocyte maturation stages provides insights into the complex gene regulatory networks that drive oocyte maturation and fertilization.</P>

A Genetic Heterogeneity of Renpenning Syndrome Mapped to Chromosome Xq21-Xqter

Cui, Bin,Sun, Yuming,Sun, Yuqin,Shi, Yuefeng,Pei, Gang,Kong, Xiangyin,Hu, Landian 한국유전학회 2004 Genes & Genomics Vol.26 No.1

Renpenning sysndrome is an X-linked mental retardation associated with short stature, moderate microcephaly, unremakdable facies, and no other neurological abnormality. Renpenning first reported this disorder in a Mennonite family, and using this family, Renpenning Syndrome (RENS) has been mapped to Xp11.2-p11.4 Xu Cs found a Chinses family with an X-linked hereditary disease, and the clinical phenotype is similar to RENS. Recently, we performed the linkage analysis, and the result has shown ht egenetic heterogeneity of Renpenning Syndrome: a suggestive novel locus mapped to Xq21-Xqter in this Chinese family.

A Genetic Neterogenecity of Renpenning Syndrome Mapped to Chromosome Xq21-Xqter

Bin Cui,Yuming Sun,Yuqin Sun,Yuefeng Shi,Gang Pei,Xiangyin Kong,Landian Hu 한국유전학회 2004 Genes & Genomics Vol.26 No.1

Sulforaphane Ameliorates Diabetes-Induced Renal Fibrosis through Epigenetic Up-Regulation of BMP-7

Lili Kong,Hongyue Wang,Chenhao Li,Huiyan Cheng,Yan Cui,Li Liu,Ying Zhao 대한당뇨병학회 2021 Diabetes and Metabolism Journal Vol.45 No.6

Background: The dietary agent sulforaphane (SFN) has been reported to reduce diabetes-induced renal fibrosis, as well as inhibit histone deacetylase (HDAC) activity. Bone morphologic protein 7 (BMP-7) has been shown to reduce renal fibrosis induced by transforming growth factor-beta1. The aim of this study was to investigate the epigenetic effect of SFN on BMP-7 expression in diabetes-induced renal fibrosis.Methods: Streptozotocin (STZ)-induced diabetic mice and age-matched controls were subcutaneously injected with SFN or vehicle for 4 months to measure the in vivo effects of SFN on the kidneys. The human renal proximal tubular (HK11) cell line was used to mimic diabetic conditions in vitro. HK11 cells were transfected to over-express HDAC2 and treated with high glucose/palmitate (HG/Pal) to explore the epigenetic modulation of BMP-7 in SFN-mediated protection against HG/Pal-induced renal fibrosis.Results: SFN significantly attenuated diabetes-induced renal fibrosis in vivo. Among all of the HDACs we detected, HDAC2 activity was markedly elevated in the STZ-induced diabetic kidneys and HG/Pal-treated HK11 cells. SFN inhibited the diabetes-induced increase in HDAC2 activity which was associated with histone acetylation and transcriptional activation of the BMP-7 promoter. HDAC2 over-expression reduced BMP-7 expression and abolished the SFN-mediated protection against HG/Pal-induced fibrosis in vitro.Conclusion: Our study demonstrates that the HDAC inhibitor SFN protects against diabetes-induced renal fibrosis through epigenetic up-regulation of BMP-7.

Mineralization of calcium phosphate in reverse microemulsion

Xiang-Dong Kong,Xiao-Dan Sun,Jun-Biao Lu,Fu-Zhai Cui 한국물리학회 2005 Current Applied Physics Vol.5 No.5

In the present study, reverse microemulsion was prepared to regulate the mineralization of calcium phosphate. Calcium chlorideand sodium hydrogen phosphate aqueous solution were, respectively, dropped into the mixture of Span 80, Tween 80, andn-heptaneto get two kinds of emulsions.n-Buranol was used to adjust the emulsions to transparent state. Calcium phosphate was prepared byadding phosphate microemulsion to Calcium microemulsion. Heating method was used to induce the phase separation. After beingwashed with acetone and ethanol, the mineralized deposition were studied with scanning electron microscopy (SEM), X-ray dirac-tion (XRD) and transmission electron microscopy (TEM). The results indicated that the minerals in the deposition were mainlymonetite (DCPA) and the nanosized needle-like DCPA exhibited the preferential orientation along 200 direction..

Research progress of defective MoS2 for photocatalytic hydrogen evolution

Liu Chao,Kong Cui,Zhang Feng-Jun,Kai Chun-Mei,Cai Wei-Qin,Sun Xian-Yang,오원춘 한국세라믹학회 2021 한국세라믹학회지 Vol.58 No.2

The potential application of MoS 2 as a potential H 2 precipitation photocatalyst has received widespread attention and is considered a promising alternative to precious metal cocatalysts due to its richness and low cost. However, the catalytic active center of MoS 2 is only along the edge of the MoS 2 layer. Both theoretical and experimental studies have shown that defect engineering can increase the active site of MoS 2 and has superior activity in catalytic reactions. Therefore, this review describes the nature, defect types, and preparation of defective MoS 2 . Due to the recombination of MoS 2 and semiconductor has specifi c interface characteristics, Schottky heterojunctions can provide accelerated charge separation and lower Schottky barriers for photocatalytic applications, they are eff ective photocatalysts. Therefore, the preparation of the defect MoS 2 -supported semiconductor photocatalyst and its application in the photocatalytic water splitting reaction are also introduced. This article’s profound understanding of defects can consolidate basic photocatalysis theory and provide new insights for the rational design of satisfactory defect engineering photocatalytic materials.

Visual Target Tracking Algorithm via Multi-scale Block and Sparse Representation

Ming Li,Cui-Cui Kong,Fu-Zhong Nian,Lei Wang 보안공학연구지원센터 2015 International Journal of Signal Processing, Image Vol.8 No.8

In this paper, we propose a novel algorithm to deal with the problem of visual tracking in some challenging situations, which is based on sparse representation and multi-scale block. To build target templates, we select distinguishable features between the target and background in each frame of video sequences, dictionary is built by the multi-scale block of target templates. Then, particle filter generates filter distribution in the next frame, the moving target is framed by affine transformation. To describe the current state of the target, we calculate posterior probability for each particle. Finally, the templates are updated online. The experimental results show that the proposed algorithm is superior in accuracy than the classical tracking algorithm, and it has better robustness against to the target posture changes, partial occlusion and illumination variations.

Cat fertilization by mouse sperm injection

Jin, Yong-Xun,Cui, Xiang-Shun,Yu, Xian-Feng,Lee, Sung-Hyun,Wang, Qing-Ling,Gao, Wei-Wei,Xu, Yong-Nan,Sun, Shao-Chen,Kong, IL-Keun,Kim, Nam-Hyung Cambridge University Press 2012 Zygote Vol.20 No.4

<B>Summary</B><P>Interspecies intracytoplasmic sperm injection has been carried out to understand species-specific differences in oocyte environments and sperm components during fertilization. While sperm aster organization during cat fertilization requires a paternally derived centriole, mouse and hamster fertilization occur within the maternal centrosomal components. To address the questions of where sperm aster assembly occurs and whether complete fertilization is achieved in cat oocytes by interspecies sperm, we studied the fertilization processes of cat oocytes following the injection of cat, mouse, or hamster sperm. Male and female pronuclear formations were not different in the cat oocytes at 6 h following cat, mouse or hamster sperm injection. Microtubule asters were seen in all oocytes following intracytoplasmic injection of cat, mouse or hamster sperm. Immunocytochemical staining with a histone H3-m2K9 antibody revealed that mouse sperm chromatin is incorporated normally with cat egg chromatin, and that the cat eggs fertilized with mouse sperm enter metaphase and become normal 2-cell stage embryos. These results suggest that sperm aster formation is maternally dependent, and that fertilization processes and cleavage occur in a non-species specific manner in cat oocytes.</P>