Vascular Protective Role of Samul-Tang in HUVECs: Involvement of Nrf2/HO-1 and NO

Choi, Eun Sik,Lee, Yun Jung,Seo, Chang Seob,Yoon, Jung Joo,Han, Byung Hyuk,Park, Min Cheol,Kang, Dae Gill,Lee, Ho Sub Hindawi Publishing Corporation 2016 Evidence-based Complementary and Alternative Medic Vol.2016 No.-

<P>Samul-Tang (Si-Wu-Tang, SMT), composed of four medicinal herbs, is a well-known herbal formula treating hematological disorder or gynecologic disease. However, vascular protective effects of SMT and its molecular mechanisms on the vascular endothelium, known as the central spot of vascular inflammatory process, are not reported. The aim of this study was to investigate vascular protective effects of SMT water extract in human umbilical vein endothelial cells (HUVECs). Water extract of SMT was prepared and identified by HPLC-PDA analysis. Expression of cell adhesion molecules (CAMs) and heme oxygenase-1 (HO-1) and translocation of nuclear factor-kappa B (NF-<I>κ</I>B) and nuclear factor-erythroid 2-related factor 2 (Nrf2) were determined by western blot. Nuclear localization of NF-<I>κ</I>B and Nrf2 was visualized by immunofluorescence and DNA binding activity of NF-<I>κ</I>B was measured. ROS production, HL-60 monocyte adhesion, and intracellular nitric oxide (NO) were also measured using a fluorescent indicator. SMT suppressed NF-<I>κ</I>B translocation and activation as well as expression of CAMs, monocyte adhesion, and ROS production induced by TNF-<I>α</I> in HUVECs. SMT treated HUVECs showed upregulation of HO-1 and NO which are responsible for vascular protective action. Our study suggests that SMT, a traditionally used herbal formula, protects the vascular endothelium from inflammation and might be used as a promising vascular protective drug.</P>

치주적 손상을 유발하는 latex-elastic ring의 부적절한 사용에 대한 증례보고

최원경,손흥규,최병재,이제호 大韓小兒齒科學會 1997 大韓小兒齒科學會誌 Vol.24 No.3

This paper presents improper use of latex-elastic ring in movement of teeth and its consequence. Simple orthodontic movement of teeth including closure of diastema may be achieved by the use of simple method with fixed or removable appliance associated with latex-elactic ring. But, if it is used alone, it acts as a local irritant and then produces localized periodontitis. In case of localized periodontitis resulting from latex-elastic ring, diagnosis is difficult because the patient is usually unaward of the presence of the rings on the roots of the involved teeth and because the ring is not probed and is radiolucent and therefore not discernible radiographically and clinically. Occasionally, surgery is requred for the detection. This case emphasizes the necessity that the use of latex-elastic ring in closing diastema should be accompanied with removable or fixed appliance as well as close professional supervision. Its use without adequate provision for stabilization or retention on the crowns of teeth results in destruction of periodontal support and the teeth may be lost.

Open-face Stainless Steel Crown을 이용한 유전치의 치료에 대한 증례보고

최형준,이제호,최병재,김종민 大韓小兒齒科學會 1997 大韓小兒齒科學會誌 Vol.24 No.4

In spite of the improvements of the techniques in the field of preventive dentistry, many children still present with extensive destruction of primary anterior teeth. Not only the practioner must consider the pulp state of the primary incisor, but also restore the form, function and esthetics of the tooth. Restorative treatment of primary incisor tooth requires durability, retention and esthetics. Stainless steel crowns used in restoring primary anterior teeth is retentive and durable in comparison with the composite resin, celluloid crown. But they are not esthetic. To enhance the esthetics of the anterior stainless steel crown without reducing its superior retention, an open-face stainless steel crown has been suggested. Several authors have suggested cutting away the labial portion of the stainless steel crown and placing the composite resin in that area. By following this technique, the practioner can prepare a retentive, durable, and esthetic restoration for primary teeth which have suffered from extensive loss of teeth structure. In addition, the single missing primary anterior teeth can be successfully restored by soldering the stainless steel crown together. Open-face stainless steel crown is indicated in the areas of large interproximal lesions involving incisal edge, crown fracture with pulp exposure and congenital malformation of the teeth. By this technique, the practioner can restore primary anterior teeth successfully regardless of the amount of remaining tooth structure, bruxism habit and presence of attrition. In this case, rampant caries with extensive loss of tooth structure and single missing of primary anterior tooth hart been successfully treated with open-face stainless steel crown.

Hypophosphatasia 환아의 치료 증례

최병제,최형준,이제호,김기덕,박수정 大韓小兒齒科學會 1998 大韓小兒齒科學會誌 Vol.25 No.3

1. Hypophosphatasia 는 골조직 및 치아조직의 비정상적인 석회화를 나타내는 대사장애로, 특징적구강내 소견 인 우치의 조기탈락 양상으로 치과에서 조기진단이 가능하다. 2. 본 증례에서 상악궁 확장을 동반한 의치 제작으로 심미적, 기능적으로 양호한 결과를 얻었으며, 영구치열기 까지 장기적 관찰 및 치료가 필요할 것으로 사료된다. hypophosphatasia is a rare metabolic disorder which manifests characteristics such as abnormal mineralization of bone and dental tissues, diminished serum and tissue alkline phosphatase, and increased urinary secretion of PEA. It ingerited as an autosomal recessive or dominant trait and occurs in all races. In general, hypophosphatasia can be classified in 4 subtypes which are the perinatal, infantile, childhood, adult type depending upon the age at presentation and severity. In young children with Hypophosphatasia the long bones show irregular defects, and the skull showes poor calcification. In older children with premature closure of the skull sutures there may ve multiple lucent area called gyral or convolutional markings, described as resimbling beaten copper, presumably resulting from increased intracranial pressure,Examination of the jaws reveals a generalized lucency of the maxilla and mandible, the cortical bone and lamina dura are thin, and the alveolar bone may be deficient. Clinical features of Hypophosphatasia imclude premature loss of deciduous teeth, especially incisors, hypoplasia of aplasia of root cementum, enamel hypoplasia, irregular calcification of dentin, large pulp chamber, and resorption of marginal alveolar bone and roots. Our report involves a patient with a chief complaint of early loss of both Mx. and Mn. deciduous ncisors. after conducting a through clinical and radiographic examination this patient was referred to pediatrics under the suspicion of hypophosphatasia, the diagnosis proved to be correct and successful results were accomplished through a denture made to improve esthetics and function.

古里海域의 潮流分析

崔秉昊 成均館大學校 科學技術硏究所 1984 論文集 Vol.35 No.1

우리나라 東南部 古里海域에서 1978年 9月 4日부터 10月 3日까지 自動流速計에 依해 觀測된 海流記錄이 一貫된 分析體系를 通해 分析되었다. 觀測海流 및 恒流의 時計方向, 反時計方向 回轉스펙트럼을 求하기 爲하여 回轉成分方法이 利用되었다. 潮流의 主要分潮의 調和常數를 求하기 爲한 潮流分析이 遂行되었다. 硏究의 窮極的인 目的은 追後 黃海 및 東支那海의 海流觀測資料를 整然하게 處理하여 陸棚力學硏究에 寄與하는데 있다. A Current meter data recorded from 4 September to 3 October, 1978 at Kori Point, southeastern part of Korea was processed in systematic manner and analyzed with rotary component method to provide clockwise and anti-clockwise rotary spectra. A tidal analysis was performed to estimate harmonic constant of principal components of the tidal current.

光陽灣의 二次元 潮汐 數値模型

崔秉昊 成均館大學校 科學技術硏究所 1990 論文集 Vol.41 No.1

A two-dimensional hydrodynamics-numerical model was established to investigate the hydraulic process in the vicinity of large steel mill cnstructed on the reclaimed platform at the estuary of Sumjin River, Kwang Yang Bay. The developed model was first used to simulate M_2 tide in the estuary. The computed results were presented in the form of tidal ellipses, maximum bottom stress and energy dissipation and discussions were also made for furture development of the model.

Ca 첨가한 AZ91 마그네슘 합금 용탕의 산화거동

최병호,유봉선,박원욱,박익민 대한금속재료학회 2004 대한금속·재료학회지 Vol.42 No.8

The effects of Ca addition on the liquid oxidation behaviors of AZ91 alloys have been investigated. The oxide layers formed on the molten Ca-containing AZ91 alloys were investigated by using scanning electron microscope and Auger electron spectroscopy in order to elucidate the effect of Ca additions on the ignition and burning of the molten alloys. The thermogravimetric measurements indicated that Ca-containing AZ91 alloys have good oxidation resistance. AES depth analysis revealed that the oxide layers on the molten Ca-containing AZ91 alloys consisted of several layers; the outer layer mainly consisted of CaO and the inner layer was mainly a mixture of CaO-MgO and Al₂O₃. The formation of protective CaO in the oxide layer was directly responsible for improved oxidation resistance of the Ca-containing AZ91 alloys.

殘留應力을 考慮한 鎔接材의 疲勞龜裂 進展擧動에 關한 硏究

최병기,이을호,국중민,기준호,임선빈,정장만 朝鮮大學校 機械技術硏究所 2000 機械技術硏究 Vol.3 No.1

The effect of welding residual stress on the initiation and propagation of fatigue crack is investigated so as to study the fatigue behavior in welding residual stress field. Especially, the relation between welding residual stress and fatigue crack opening behavior is investigated and the redistribution of residual stress as a fatigue crack propagates is examined. Following results are obtained. 1) In case of the load range is constant, as the stress ratio is changing to 0.1, 0.33, 0.5 the propagation life is constant but the growth life decreases. And than, when maximum load or minimum load is constant, as the stress ratio increases the growth life and propagation life increase. 2) The fatigue crack propagations rate da/dN appears to accelerate when the stress intensity factor range ΔK is less than 15MPa-m^1/2. 3) The residual stress that is appeared by welding decreases after cutting. 4) Fatigue crack propagation ratio is affected by tensional and compressible residual stress at the initial time, but it's similar to the original in the more than 20mm of crack length.

확장된 칼만필터를 이용한 유도전동기의 센서리스 벡터제어

최연옥,정병호,조금배 조선대학교 에너지.자원신기술연구소 2000 에너지·자원신기술연구소 논문지 Vol.22 No.1

Using Observer, on the sensorless vector control system is a novel techniques for modern induction motor control. In this paper, a speed estimation algorithm of an induction motor using an extended kalman filter was proposed. Extended kalman filter can solve the problem, that have steady state error of estimated speed in flux and slip estimation method. The extended Kalman filter is employed to identify the speed of an induction motor and rotor flux based on the measured quantities such as stator current and DC link voltage. In order to confirming above proposal, computer simulation carried out control drives for using Matlab Simulink and show the effectiveness of the control drives for induction motor speed estimation.

구강점막의 배양에 관한 연구

최병호,유재하 大韓顎顔面成形再建外科學會 1995 Maxillofacial Plastic Reconstructive Surgery Vol.17 No.4

To use cultured mucosa as a graft of full thickness, our laboratory has been involved in the development of techniques to grow epidermis together with connective tissue. Human oral mucosa was obtained at dental surgery. Under sterile conditions the tissues were cut into explants of 0.1 cm² which were placed in the center of 24 well tissue culture dishes and incubated in a growth medium. The growth medium used for epithelial was MEM(Minimum Essential Medium) supplemented with 10% fetal calf serum, 0.5% dimethyl sulfoxide, glutamine (0.292 g/l), epidermal growth factor (40 ug/ml), cholera toxin (30 ng/ml), hydrocortisone(2 ug/ml), insulin (40 ug/ml) and transferin (5 ug/ml). The medium for stratification of epithelial cells was MEM supplemented with 10% fetal calf serum, 0.5% dimethyl sulfoxide and glutamine (0.292 g/l). The medium used for fibroblasts was MEM supplemented with 10% fetal calf serum. With the three types of media used alternatively, a mucosa composed of epidermis and connective tissue was obtained after 3 weeks of culture.