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Isoflavone profile diversity in Korean wild soybeans ( Glycine soja Sieb. & Zucc.)
TSUKAMOTO, Chigen,NAWAZ, Muhammad Amjad,KUROSAKA, Ayaka,LE, Bao,LEE, Jeong Dong,SON, Eunho,YANG, Seung Hwan,KURT, Cemal,BALOCH, Faheem Shehzad,CHUNG, Gyuhwa Scientific and Technological Research Council of T 2018 Turkish journal of agriculture & forestry Vol.42 No.4
Itabashi, Mei,Tsukamoto, Chigen,Kurosaka, Ayaka,Krishnamurthy, Panneerselvam,Shin, Tai-Sun,Yang, Seung Hwan,Son, Eunho,Chung, Gyuhwa Marcel Dekker 2016 JOURNAL OF LIQUID CHROMATOGRAPHY AND RELATED TECHN Vol.39 No.14
<P>New saponin components, Sg-6 saponins, have been recently reported from the seeds of wild soybean (Glycine soja) which may have specific health benefits. To evaluate the possible health benefits, a large amount of Sg-6 saponins are needed, but general group A acetyl saponins and new Sg-6 saponins are eluted in overlapping peaks by ordinal preparative high-performance liquid chromatography and/or open column methods. A new method is proposed in this report. This method includes (1) deacetylation of group A acetyl saponins in alkali condition with KOH, (2) precipitation of Sg-6 saponins in acid condition with HCl, (3) recovery of Sg-6 saponins with aqueous methanol from the precipitate, and (4) elution of Sg-6 saponins by preparative reverse-phase open column. With this method, from 450g of wild soybean whole seed powder, about 1g of Sg-6 saponins (mixture of six components) was clearly separated from other saponins with 61% recovery. [GRAPHICS] .</P>
Sun Kyung Oh(오선경),Chigen Tsukamoto,Ki Woong Kim(김기웅),Myeong Rak Choi(최명락) 한국생물공학회 2016 KSBB Journal Vol.31 No.1
Changes in the concentrations of glucosinolates and related compounds in different extracts of Dolsan leaf mustard kimchi (DLMK) and Dolsan leaf mustard pickles (DLMP) were during storage investigated. Samples were kept at 0℃ for 35 days and collected at 7 day intervals. The leaves and stems of DLMK and DLMP were refluxed for 24 h with 50% acetonitrile, and the extracts were analyzed by LC-PDA/MS/MS. The main glucosinolates detected in DLMK were sinigrin, gluconapoleiferin, glucobrassicanapin, and gluconapin, whereas those in DLMP were sinigrin, gluconapoleiferin, glucobrassicanapin, glucobrassicin, and glucoerucin. Sinigrin concentrations were quantified by UV absorption at 228 nm. Sinigrin concentrations in the leaves and stems of DLMK on the day of preparation were 2.14 mg/g and 2.25 mg/g, respectively, and those on day 35 after preparation were 1.25 mg/g and 1.00 mg/g, respectively. DLMP showed a similar trend: the concentrations in the leaves and stems on the day of preparation were 2.04 mg/g and 0.29 mg/g, respectively, whereas those on day 35 after preparation were 0.59 mg/g and 0.41 mg/g, respectively. Thus, sinigrin concentrations decreased during storage.
오선경,김기웅,최명락,Oh, Sun Kyung,Tsukamoto, Chigen,Kim, Ki Woong,Choi, Myeong Rak The Korean Society for Biotechnology and Bioengine 2016 KSBB Journal Vol.10 No.5
Changes in the concentrations of glucosinolates and related compounds in different extracts of Dolsan leaf mustard kimchi (DLMK) and Dolsan leaf mustard pickles (DLMP) were during storage investigated. Samples were kept at 0oC for 35 days and collected at 7 day intervals. The leaves and stems of DLMK and DLMP were refluxed for 24 h with 50% acetonitrile, and the extracts were analyzed by LC-PDA/MS/MS. The main glucosinolates detected in DLMK were sinigrin, gluconapoleiferin, glucobrassicanapin, and gluconapin, whereas those in DLMP were sinigrin, gluconapoleiferin, glucobrassicanapin, glucobrassicin, and glucoerucin. Sinigrin concentrations were quantified by UV absorption at 228 nm. Sinigrin concentrations in the leaves and stems of DLMK on the day of preparation were 2.14 mg/g and 2.25 mg/g, respectively, and those on day 35 after preparation were 1.25 mg/g and 1.00 mg/g, respectively. DLMP showed a similar trend: the concentrations in the leaves and stems on the day of preparation were 2.04 mg/g and 0.29 mg/g, respectively, whereas those on day 35 after preparation were 0.59 mg/g and 0.41 mg/g, respectively. Thus, sinigrin concentrations decreased during storage.
Hae-Reon Son(손혜련),Sun-Kyung Oh(오선경),Chigen Tsukamoto,Myeong-Rak Choi(최명락) 한국생물공학회 2016 KSBB Journal Vol.31 No.3
This study was carried out for investigate that physiological activity, quantification and qualitative were sinigrin of Dolsan leaf mustard pickle (DLMP) during storage. sinigrin contains high amounts of nutritional and medicinal compounds, which are important for maintaining optimum health. ACE inhibitory activity was ranged between 43.2 and 79.4%. DLMP methanol extracts demonstrated highest ACE inhibitory activity at 79.4% on day 14, whereas DLMP ethanol extracts demonstrated highest Angiotensin I-converting Enzyme (ACE) inhibitory activity of 43.2% at day 0. The α-glucosidase inhibitory activity of positive control 0.02% (v/v) acarbose was 78%. The DLMP methanol extracts had the highest α-glucosidase inhibitory activity at 64.0% on day 14, whereas DLMP ethanol extracts had the lowest α-glucosidase inhibitory activity of 42.8% at day 28. Sinigrin was high in DLMP methanol extracts at 49.55 μg/ml on day 14 of storage. Sinigrin standard was eluted at 2.73 min and MS analysis was m/z 283.03 along with fragment ions at m/z 204 and 149.06. These data show that sinigrin formed desulfo-glucosinolates [M-SO3-2H2O+K+2H]+. Sinigrin concentration increased until day 14 and then decreased after that. DLMP methanol extracts had consistently higher sinigrin concentration than DLMP acetonitrile extracts during 28 days of storage.