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Park Cheol,Lee Hyesook,Kim Sung Ok,Lee Eun-Woo,Lee Hyun-Tai,Kwon Hyun Ju,Kim Byung Woo,Kim Gi-Young,Kim Mi Ryeo,Choi Yung Hyun 한국독성학회 2023 Toxicological Research Vol.39 No.1
The aim of the present study is to investigate the preventive effect of water extract of Mori Ramulus (MRWE) on oxidative stress-mediated cellular damages in rat skeletal L6 myoblasts. Our results demonstrated that MRWE pretreatment markedly improved cell survival and suppressed cell cycle arrest at the G2/M phase and apoptosis in hydrogen peroxide ( H2O2)-treated L6 cells. H2O2- triggered DNA damage was also notably reduced by MRWE, which since it was correlated with protection of reactive oxygen species (ROS) production. Additionally, H2O2 stimulated cytosolic release of cytochrome c and upregulation of Bax/Bcl-2 ratio, whereas MRWE suppressed these changes following by H2O2. Moreover, MRWE inhibited the cleavage of poly(ADP-ribose) polymerase as well as the activity of caspase-3 by H2O2. Furthermore, MRWE enhanced H2O2- mediated expression of nuclear factor erythroid 2-associated factor 2 (Nrf2) and its representative downstream enzyme, heme oxygenase-1 (HO-1). However, the protective effects of MRWE on H2O2- induced ROS production, cell cycle arrest and apoptosis were significantly attenuated by HO-1 inhibitor. In conclusion, our present results suggests that MRWE could protect L6 myoblasts from H2O2- induced cellular injury by inhibiting ROS generation along with Nrf2-mediated activation of HO-1, indicating this finding may expand the scope of application of Mori Ramulus in medicine.
External validation of IBTR! 2.0 nomogram for prediction of ipsilateral breast tumor recurrence
Byung Min Lee,Jee Suk Chang,Young Up Cho,Seho Park,Hyung Seok Park,Jee Ye Kim,Joo Hyuk Sohn,Gun Min Kim,Ja Seung Koo,Ki Chang Keum,Chang-Ok Suh,Yong Bae Kim 대한방사선종양학회 2018 Radiation Oncology Journal Vol.36 No.2
Purpose: IBTR! 2.0 nomogram is web-based nomogram that predicts ipsilateral breast tumor recurrence (IBTR). We aimed to validate the IBTR! 2.0 using an external data set. Materials and Methods: The cohort consisted of 2,206 patients, who received breast conserving surgery and radiation therapy from 1992 to 2012 at our institution, where wide surgical excision is been routinely performed. Discrimination and calibration were used for assessing model performance. Patients with predicted 10-year IBTR risk based on an IBTR! 2.0 nomogram score of 〈3%, 3%–5%, 5%–10%, and 〉10% were assigned to groups 1, 2, 3, and 4, respectively. We also plotted calibration values to observe the actual IBTR rate against the nomogram-derived 10-year IBTR probabilities. Results: The median follow-up period was 73 months (range, 6 to 277 months). The area under the receiver operating characteristic curve was 0.607, showing poor accordance between the estimated and observed recurrence rate. Calibration plot confirmed that the IBTR! 2.0 nomogram predicted the 10-year IBTR risk higher than the observed IBTR rates in all groups. High discrepancies between nomogram IBTR predictions and observed IBTR rates were observed in overall risk groups. Compared with the original development dataset, our patients had fewer high grade tumors, less margin positivity, and less lymphovascular invasion, and more use of modern systemic therapies. Conclusions: IBTR! 2.0 nomogram seems to have the moderate discriminative ability with a tendency to over-estimating risk rate. Continued efforts are needed to ensure external applicability of published nomograms by validating the program using an external patient population.
Stability Evaluation of National Reference Standards for Blood Products in Korea
Park, Tae Jun,Choi, Chan Woong,Oh, Ho Kyung,Kim, Jae Ok,Kim, Byung Kuk,Kang, Hyun Kyung,Kwon, Eun Jeong,Gweon, Eun Jeong,Park, Sang Jin,Kang, Ho Il,Jung, Ki Kyung,Park, Sang Mi,Kim, Ji Hye,Han, Ki Won Korean Society of ToxicologyKorea Environmental Mu 2017 Toxicological Research Vol.33 No.3
National reference standards (NRSs) for biologics are established through potency estimation by a multi-center joint study of standard materials used in the approval process for national lot release and quality control of vaccines, blood products, and other biologics. In this study, a stability evaluation was conducted to determine whether the potency of NRSs for six blood products was being maintained at a consistent level in Korea. The present study conducted real-time stability tests via in-vivo/in-vitro bioassay on NRSs for blood coagulation factor VIII concentrate (2nd standard), antithrombin concentrate, prekallikrein activator, anti-hepatitis B immunoglobulin, blood coagulation factor IX concentrate, and anti-tetanus human immunoglobulin, as well as a trend analysis using cumulative annual results. The real-time stability test results showed that the mean potency of six NRSs was all within the control limit. In the trend analysis, the potency of NRS for blood coagulation factor VIII concentrate (2nd standard) showed a decreasing trend, while the potency of all other products had been stably maintained. The present study confirmed that the mean potency of NRSs for six blood products had been stably maintained in Korea. The findings of the present study establish a foundation that can ensure the quality of NRSs for biologics in Korea, and it is expected to make a major contribution to the supply of high-quality biologics.
Park, Si-Eun,Lee, Mi-Joung,Yoon, Bum-Chul,Lee, Byung-Hee,Shin, Hee-Joon,Choi, Wan-Suk,Park, Sung-Kyu,Jeon, Hye-Mi,Moon, Ok-Kon,Lee, Suk-Hee,Min, Kyoung-Ok International Academy of Physical Therapy Research 2010 Journal of International Academy of Physical Ther Vol.1 No.2
The purpose of this study was to compare the effects of treadmill walking in underwater and overground which affects gait and physical function of people who have had a stroke. Twenty people after a stroke who have become hemiplegic over 6 months were participated. Participants were divided into two groups: underwater treadmill group(UTG) and overground treadmill group(OTG). The intervention was done 4 times per weeks for 6 weeks and 1 session lasted for 30 minutes. Gait and physical function elements were measured at baseline, at the middle(3 weeks) and at the end of the intervention(6 weeks). For the elements of gait, walking velocity, affected stance phase, affected weight bearing were assessed. For the elements of physical function, Short Form 8(SF-8) health survey was used. The result of this study showed that both groups improved similarly in walking velocity. However participants in UTG improved more than those in OTG in affected stance phase(p<.05), affected weight bearing(p<.05) and emotional aspect(p<.001). Based on the results of this study, it can be suggested that treadmill walking both in underwater and on the ground can be effective in improving hemiplegic gait and physical function of people who have had a stroke. The result also suggest that the underwater treadmill exercise can be more effective than overground treadmill in restoration of gait in people after stroke.
( Ok-hee Kim ),( Hyojung Kim ),( Jinku Kang ),( Dongki Yang ),( Yu-hoi Kang ),( Dae Ho Lee ),( Gi Jeong Cheon ),( Sang Chul Park ),( Byung-chul Oh ) 생화학분자생물학회(구 한국생화학분자생물학회) 2017 BMB Reports Vol.50 No.1
Accumulation of tissue macrophages is a significant cha-racteristic of disease-associated chronic inflammation, and facilitates the progression of disease pathology. However, the functional roles of these bone marrow-derived macrophages (BMDMs) in aging are unclear. Here, we identified age-dependent macrophage accumulation in the bone marrow, showing that aging significantly increases the number of M1 macrophages and impairs polarization of BMDMs. We found that age-related dysregulation of BMDMs is associated with abnormal overexpression of the anti-inflammatory interleukin-10. BMDM dysregulation in aging impairs the expression levels of pro-inflammatory cytokines and genes involved in B-cell maturation and activation. Phagocytosis of apoptotic Jurkat cells by BMDMs was reduced because of low expression of phagocytic receptor CD14, indicating that increased apoptotic cells may result from defective phagocytosis of apoptotic cells in the BM of aged mice. Therefore, CD14 may represent a promising target for preventing BMDM dysregulation, and macrophage accumulation may provide diagnostic and thera-peutic clues. [BMB Reports 2017; 50(1): 43-48]
Park, Jung Eun,Choi, Byung Ok,Lee, Kyung Ah,Kang, Suk-Yun,Lee, Sook Hwan,Lee, Suman 한국유전학회 2002 Genes & Genomics Vol.24 No.3
Muscular Myotonic dystrophy (DM), the most prevalent muscular disorder in adults, is associated with an expansion of CTG repeats in the 3'-untranslated region of the DM protein kinase gene. It is characterized by a series of typical morphological events; especially the type I-specific fiber atrophy being a frequently observed symptom. The occurrence of apoptosis in skeletal muscle in DM conditions has been reported although the pathogenic molecular mechanism for DM still remains obscure. We performed TUNEL assay for detection of apoptotic cells using muscle biopsies to find specific apoptotic pattern in a DM patient. Obtained data indicated that myo-nuclei are susceptible to DNA fragmentation especially in the type I muscle fibers in DM condition. To clarify any correlation between the expression level of DMPK transcripts and apoptosis, we isolated each type of cells using by laser capture microdissection (LCM) system for RT-PCR, and determined the expression levels of DMPK transcripts between the type Ⅰ and Ⅱ fibers from DM patient and normal individual. We found that the type I fibers displayed elevated levels of DMPK transcripts in DM patient. This study suggests that type Ⅰ - specific apoptosis in DM patient may be associated with the altered levels of DMPK transcripts.
Park, O Ok,Chin, Byung doo 한국화학공학회 2001 Korean Journal of Chemical Engineering Vol.18 No.1
The dynamic rheological behavior of multiphase electrorheological (ER) fluids was considered, as continuation of a previous paper [Chin and Park, 2000]. Oil-in-oil emulsions, which differ in electrical conductivity and dielectric canstant, were emplayod for an ER-active emulsion and also for a multiphase ER fluid with enhanced performance. The palyaniline particle suspension in an electric field showed viscoelastic behavior within a very limited range of strain amplitude, indicating the transition from viscoelasticity to viscoplasticity. Within the region of viscoelasticity, the linear region was restricted below the amplitude of 0.1 %, whereas the ER-active emulsions showed a rather wide linear regime. Frequency dependence of the storage shear modulus in the linear viscoelastic region revealed the typical features of an elastic solid. When the fraction of emulsion drops ( Φ) in multiphase ER fluids increased, the limiting strain for viscoelasticity showed a higher value.