국내에서 분리된 Infectious Bovine Rhinotracheitis Virus DNA의 제한효소분석

민원기,전무형,이중복,김병한,안수환 충남대학교부설 생명공학연구소 1991 생물공학연구지 Vol.1 No.-

Nine strains of infectious bovine rhinotracheitis virus(IBRV) isolated in Korea since 1970 were investigated to elucidate the pathogenicity on various cell lines and the reactivity in cross serum neutralization test. The genomes of the viruses were also studied by restriction endonuclease analysis to examine the genetical patterns and the origin of the viruses. Results obtained by experiment were as follows ; 1. In pathogenicity test on various cell lines by cytopathology and immunofluorescence antibody assay, MDBK cells were found to be very susceptible to all of the domestic isolates, Colorado and Oxford strains and RK-13 cells, mildly reactive to all of viruses. However, CV-1 and BHK cells revealed negligible reactions against all of viruses tested. 2. In cross serum neutralization(SN) test with the positive serum from the cattle immunized with PQ7 strain of IBRV, PQ7, SQ, VS, A14, TSV and S′74-7 strains showed 64 of SN titer, and IQ, QW, A37, Colorado and Oxford strains, 128 of SN titer. It was assumed that there is no difference in major antigenic determinants among the viruses. 3. By analysis of restriction endonuclease Hind Ⅲ, it was found that 8 domestic isolates except A37 and Colorado strain revealed the identical banding patterns. However, Oxford strain showed quite different patterns, displaying H band at 6.9×10 exp (6) daltons and K′band at 4.5×10 exp (6) daltons. A37 strain exclusively contained the longer bands of A′ and A″. 4. When the viral DNA were analyzed by restriction endonuclease EcoRI, all of the domestic isolates and Colorado strain showed very similar banding patterns, whereas Oxford strain revealed a considerable discrepancy, displaying B′ band at 12.5×10 exp (6) daltons. 5. By analysis of restriction endonuclease BamHI, it was found that the domestic isolates and Colorado strain revealed similar patterns displaying 8 fragments. However, Oxford strain was cleaved into 9 fragments displaying G′and G″ bands.

백서 척수후근절로부터 슈반세포의 효과적인 체외 배양법

김성민,이종호,김남열,안강민,최원재,최시호,차미주,이주영,황순정,장정원,명훈,최진영,서병무,정필훈,김명진 大韓顎顔面成形再建外科學會 2003 Maxillofacial Plastic Reconstructive Surgery Vol.25 No.4

Schwann cells(SCs), an important component of the peripheral nervous system, intract with nerous to mutually support growth and replication for the peripheral nerve regentation. Recently, ading SCs to the lumen of guidance channel is widely tried to improve regeneration or to make regeneration possible over otherwise irreparable gaps. however, it is not easy to isolate and multiplicate SCs as much as enough to help the axonal regeneration. For the allogeneic SCs source for tubular nerve guidance, we developed a little bit improved technique of harvesting and multiplicating SCs. by culturing dispersed dorsal root ganglia in specially designed medium with growth factors and serial processing, we repeatedlly generate relatively homogenous SC cultures. Our technique was compared with other methods of literature using immunostaining methods such as GFAP, S100, BDNF and the total SC count assessment at different time interval after primary culture.

EGFR 돌연변이와 ROS1 전위를 동시에 가진 폐선암 환자의 Erlotinib 치료 1예

김민환,박예현,박혜정,지아영,송창호,진무년,김영주,김선욱,이중희,김인수,김혜련,김주항,조병철 Ewha Womans University School of Medicine 2014 EMJ (Ewha medical journal) Vol.37 No.1

The rearrangement of c-ros oncogene 1 (ROS1) has been recently identified as an important molecular target in non small cell lung cancer (NSCLC). ROS1 rearrangement and epidermal growth factor receptor (EGFR) mutation were mutually exclusive each other in previous studies, and the clinical implication of co-existence of the two genetic alterations has not been determined. We report a case of 46-year-old female never-smoker NSCLC patient whose tumor harbored ROS1 rearrangement and EGFR mutation concomitantly. She had undergone curative surgery for stage IIIA NSCLC, and the recurrence in left pleura and brain occurred at 2 years after the surgery. She received several lines of chemotherapy including docetaxel plus carboplatin, erlotinib, pemetrexed, and gemcitabine. Erlotinib therapy showed a favorable treatment response with progression-free survival of 9.5 months and partial response of tumor on radiologic evaluations. This case represents a successful erlotinib treatment in a NSCLC patient with concurrent ROS1 rearrangement and EGFR mutation.

Goretex를 이용한 융비술

최진영,민철기,명훈,황순정,서병무,이종호,정필순,김명진 大韓顎顔面成形再建外科學會 2003 Maxillofacial Plastic Reconstructive Surgery Vol.25 No.4

융비술시 실리콘 고무는 다루기 쉽고 조직 반응이 적어 현대 많이 돌출의 합병증이 생길 수 있다. 저자등은 이러한 실리콘의 단점을 보완할 수 있는 goretex를 사용하여 융비술을 시행하고 비첨에 대해서 자가연골을 이식하여 만족할 만한 결과를 얻었기에 goretex가 안면부 연조직의 증강을 위한 이식재료로 적합하다고 생각되어 문헌고찰과 함께 보고하는 바이다. Many different materials have been used to provide augmentation of the nasal dorsum. this include both autogenous and homogenous materials,as well as allografts. Silicone among the artificial materials has been most widely used. The silicone has some advantages and disadvantages. Goretex(expanded polytetra-fluoroethylene) which has widely used in vascular surgery and abdominal surgery can be used for facial soft tissue augmentation. The author presemts 2 cases of nasal augmentaion using goretex with literature review.

榮山江 유역의 洪水被害潛在能 산정

김운중,민병무 朝鮮大學校 建設技術硏究所 2007 建設技術硏究 Vol.27 No.1

In this study the potential flood damagef (PFD) of Young-San river basin is analysed on 32 sub regions divided by the tributary characteristics of the whole basin. The analysis of PFD is based on the factors about the possibilities, the potentials of flood events and the weakness of protection facilities. PFD is proposed for the establishment of the degree of safety for flood protection and is applied for the investment schedule of flood protection facilities. The results of this study on PFD could be used for deciding the priorities of the each flood protection plans and for flood management of the river basin.

가동보 설치에 따른 하상변동 예측에 관한 연구

김운중,문수회,김영균,민병무 朝鮮大學校 建設技術硏究所 2007 建設技術硏究 Vol.27 No.1

To estimate the change of riverbed geography by the operation of movable weir, two-dimensional RMA-2 and SED-2D were used in this study. Hydraulic elements like flowrate or depth were simulated by RMA-2 and the change of riverbed geography according to sediment transport was simulated by SED-2D. Suktop stream was chosen as a project river for the simulation. Under the condition of 50-year rainfall frequency, the estimated flood of Suktop was 123cms. At the points of movable weir the simulation results showed that the difference of high water level from the result of HEC-RAS was less than 0.64m. And the difference of water velocity was less than 0.21m/sec. Based on the sediment simulation by SED-2D, predicted riverbed elevation was 29.70-30.22m at the point of ST1 and 28.26~28.82m at the point of ST2. Measured riverbed elevation was 29.70-30.16m, 28.28-28.69m, respectively. So, the predicted elevations are closely similar to the measured elevation. At the point of ST1, sediment transport simulation results showed that erosion of riverbed was predicted by the operation of movable weir. This erosion was ranged up to 200m upstream and the riverbed would be eroded away as much as 0.07m for one year. At the point of ST2, upstream of movable weir would be eroded and the erosion depth was estimated 0.261m. The erosion was higher at the point of movable weir and the erosion depth was estimated 0.310m. But the sediment would be deposit downstream of the movable weir. SED-2D followed by RMA-2 is well applicable to the stream which has movable weirs and can estimate not only hydraulic parameters such as flowrate or depth but also riverbed elevation change caused by erosion or deposit of fluvial sediment.

비복신경이식을 이용한 긴 거리의 말초신경 결손부 수복

이종호,이세영,김명진,이은진,안강민,김성민,최원재,명훈,황순정,서병무,최진영,정필훈 大韓顎顔面成形再建外科學會 2003 Maxillofacial Plastic Reconstructive Surgery Vol.25 No.2

The surgery of oral and maxillofacial area poses the risk of cranial nerve damage such as trigeminal nerve or facial nerve. Inferior alveolar nerve is prone to damage in the third molar extraction, implant installation, orthognathic surgery, open reduction and rigid fixation, and tumor ablation surgery. On the other hands,facial nerve is likely to be damaged or sacrificed with trauma or parotidectomy. In case of inferior alveolar nerve injury, the incidence is reported to be about 1.3%. The nerve function will almost recover in minimal damage, but it won't recover at last in total damage of a part of nerve unit. In latter cases, nerve regeneration in intended by allograft as nerve substitute or various route of merve condit. But the recovery with autograft is believed to be most relialbe mrthod in the rapair of long-span(longer than 15㎜)nerve defect. We have performed autologous sural nerve graft in the repair of nerve defect, which is caused by resection of benign or malignant tumor. Hereby we report the method of nerve harvesting, recovery of defected peripheral nerve and the complications of donor site with the discussion of sural nerve anatomy.

상온 유동층에서 층내 수평전열관의 마모특성

한근희,김용철,류정인,선도원,민병무 한국화학공학회 2004 Korean Chemical Engineering Research(HWAHAK KONGHA Vol.42 No.2

대부분의 유동층 연소장치에서 충물질과 비산된 입자에 의한 충내 전열관과 water wall의 전열관 표면의 마모는 계속적으로 골칫거리가 되고 있다. 본 연구는 단면적이 0.15m×0.30m인 상온유동층장치에서 마모하기 쉬운 아크릴 관을 사용하여 유동층에 파묻힌 전열관에 대하여 유동조건과 전열관의 배열효과에 대한 실험적인 연구를 나타냈다. 유동층의 단면적은 0.15m×0.30m이고 높이는 1.0m이다. 층물질은 강모래 평균입도 0.73, 1.24mm의 두 가지와, 평균입도 0.9mm의 무연탄 희재를 사용하였다. 유동층 높이는 0.45m이었다. 마모실험은 조건마다 100시간동안 유동화속도 1.2-1.8m/s 까지 변화시켜 수행하였다. 실험결과 마모율은 유동화속도가 증가할수록 증가하였고, 공기분배기로부터 떨어진 거리가 증가할수록 증가하였다. 그리고 공기분배기로부터 높이 0.6m의 비산영역에 위치한 전열관에 심한 마모를 나타냈다. 전열관의 원주방향에 대한 마모경향은 다른 부분과 비교하여 시계방향으로 4시 방향에서 8시 방향까지 심한 마모가 나타났고, 특히 5시 방향과 7시 방향은 심한 마모를 보였다. Erosion of in-bed tubes and water wall heat transfer surfaces by bed materials and elutriated particles have persistently plagued most fluidized bed combustion (FBC) systems. This papere presents a systematic experimental study of the effects of tube arrangement and flow condition on embedded tube erosion by using erosion-prone acrylic cylinders in a 0.15m×0.30m bench-scale cold fluidized bed. The bed material was two different sizes of river sand and anthracite ash with the average diameter of 0.73, 1.24, 0.90㎜, respectively. The static bed height was 0.45m. Erosion test was performed with the variation of fluidization velocity of 1.2 to 1.8m/s for 100 hours per set. The result shows that the erosion rate increased with fluidizing velocity, with distance from the distributor. The erosion was severe at the tube located in the splash zone of 60㎝ above the distributor. The trend with radial direction shows severe erosion at the 4-8 o'clock clockwise compare to other area, especially 5 and 7 o'clock clockwise.

폴리에스테르 섬유 감량시스템용 프로세서 타이트레이터 개발

조진호,이흥락,이종현,김명남,구성모,김무영,진경찬,유병희,강민수,김국진 경북대학교 센서기술연구소 1993 연차보고서 Vol.1993 No.-

폴리에스테르 섬유 감량시스템의 제어를 위해서는 처리조 내에서 감량되는 섬유가 목표 감량치에 도달했는지를 판단해 주는 장치가 필요하다. 본 연구에서는 가성소다용액의 초농도 및 섬유 감량 정도의 정확한 측정과 감량 종료시간의 산출 및 이에 따른 종료신호를 발생할 수 있는 폴리에스테르 섬유 감량기 전용의 자동적정장치를 개발하였다. To control caustic soda treatment system for the polyester fabric reduction, we need the device to determine that the fabric has been arrived target reduction value. In this study, we develope an automatic process titrator for the caustic soda treatment system. Major functions of the target titrating system are accurate measurements of the caustic soda concentration and then producing the end point signal for caustic soda treatment system.

하치조신경 및 설신경 손상 평가를 위한 한국인 정상 성인의 하순-이부 및 혀의 감각 조사

이종호,이세영,송승일,이은진,안강민,김성민,명훈,황순정,서병무,최진영,정필훈,김명진 大韓顎顔面成形再建外科學會 2003 Maxillofacial Plastic Reconstructive Surgery Vol.25 No.2

In the head and neck area, there are so many sensory nerves, which are sometimes injuried iatrogenically or inadvertently so that involved patients complained of the loss of sensations. In such cases, it is important to judge the degree of injuries and regeneration of nerve for better diagnosis and treatment. Seddon and Sunderland's classification, which is commomly used, is focused on histological change and nerve conduction. As times goes by, it is difficult to access patient's sensory disturbance by this method. Until now, so many methods such as contract threshold, direction, two-point discrimination, pin prick, thermal discrimination and current perception threshold have been introduced for sensory evaluation. However, there hasn't been enough information regarding each methodology nor integrated standard methodology for the measurement. the purpose of this study is to get Korean adult normative sensory values of lower lip,chin and tongue using modified methods of contact thershold, ditection, two point discrimination, pin prick, thermal discrimination and assess degree of regeneration of sensory nerve damage.