The Protein and Isozyme Patterns During in vitro Plant Regeneration of Yooja (Citrus junos Sieb.) and Trifoliate Orange (Poncirus trifoliata Rafin.)

Park,Min-Hee,Jang,Hyun-Kyu,Cha,Young-Ju,Kim,Ho-Bun,Lee,Sook-Young 한국자원식물학회 2002 Plant Resources Vol.5 No.1

In this study, plant regeneration through in vitro culture from plantlet stems of Yooja (C. junos Sieb.) and trifoliate orange (P. trifoliata Rafin.) was attempted to make mass-production system of virus-free plants having the same genotype with mother plant. In order to investigate physiological change depending on the developmental stage of plant regeneration, the changes of total protein, peroxidase and esterase activity and their isozyme patterns as well were examined in 1/2 MS medium. The results are as follows : 1. The MS medium for the optimal callus induction and shoot formation was utilized. The medium was supplemented either with 2,4-D and Kinetin or with BA and NAA. The optimal concentrations were the combination of 1.0mg/ 2,4-D +0.3mg/ Kinetin and 1.0mg BA +0.3mg NAA in callus induction and shoot formation, respectively. 2. For the plant regeneration from somatic embryos, 1/2 MS medium was used with supplements of growth regulators (free, 1.0mg/ IBA +1.0mg/ BA ,0.5mg/ IBA +0.5mg/ BA). Shooting and rooting were the best in the treatment of 0.5mg/ IBA and 0.5mg/ BA combination. 3. The total protein content has a tendency of increase with the developmental stage of embryo, but it was decreased at the plantlet. Also it was the highest at 8 and 6 weeks stage in C. junos Sieb. and P. trioliata Rafin, respectively. In the SDS-PAGE pattern of protein, C. junos Sieb. showed bands of 29.0 and 40kDa at 10 weeks. The 45,66 and 97.4 kDa bands at 10 weeks of culture were shown in P. trifoliata Rafin. 4. The highest esterase activity was shown at the 6 and 8 weeks of culture in C.junos Sieb. and P. trifoliata Rafin.., respectively. 5. Esterase isozyme patterns were shown difference according to the developmental stage. In C. junos Sieb. a new band was observed at pl 7.7 following 4 weeks culture. On the other hand, new bands in P. trifoliata Rafin. were observed at pl 7.5~6.5 following 4 and 6 weeks culture, respectively.

전이성십이지장 내분비암으로 항암화학요법 시행 받고 완전 관해 1년 후 발생한 십이지장 선암 1예

( Bun Kim ),( Ji Hye Heo ),( Joo Won Jung ),( Moon Jae Chung ),( Jeong Youp Park ),( Seung Min Bang ),( Si Young Song ),( Seung Woo Park ) 대한내과학회 2011 대한내과학회 추계학술대회 Vol.2011 No.1

위장관내분비암으로 치료 후 동일 부위에서 선암이 발생한 예는 극히 드물다. 저자들은 전이성 십이지장 내분비암으로 항암치료 받고 완전 관해 1년 후 같은 부위에서 선암이 발생하여 보고하는 바이다. 60세 남환은과거력상 30년 전 콩팥결핵으로 결핵 치료받았다. 한달 간의 전신 무력감과 흑색변으로 2009년 6월 외부병원 내원하여 시행한 검사상 십이지장암과 간전이 의심되어 본원으로 전원되었다. 내원 당시 혈압 130/80 mmHg, 맥박 92회, 체온 36.8도이었다. 일반혈액검사상 WBC 4,730/mm3, Hb12.4 g/dL, platelet 144,000/mm3, 혈청생화학검사상 Bun/Cr 17.7/1.16 mg/dL, AST/ALT 15/13IU/L이였다. 종양표지자는 CEA 0.73 ng/mL, CA 19-9 1.8U/mL이었다. 본원에서 시행한 상부위장관내시경상 십이지장의 상부하행각에 궤양융기형 덩어리가 발견되었다. 조직검사상 십이지장에서 poorly differentiated neuroendocrine carcinoma 진단되어 십이지장 내분비암 및 간전이로 2009년 11월까지 6차례 5-FU (1000 mg/m2, Day1~3)/Etoposide(100 mg/m2, Day1~3)/Cisplatin (70 mg/mg2, Day1) 항암치료 후 영상 및 상부내시경 검사상 완전관해 소견 보였으며 이후 정기적 추적관찰 중이었다. 2011년2월 시행한 상부위장관내시 경상 이전 십이지장 병변에 새롭게 생긴 용종으로 조직 검사 시행하여 선암 진단되었다. 영상 검사상 간 S6/7,S7 두군데에 전이 의심되어 intraop RFA 고려하고 2011년 2월 28일 수술 시행하였다. 십이지장 선암은 8번, 12번, 13번 림프절 확장 소견 보여 병합절제와 췌두부 절제술을 시행하였다. 수술 중 초음파로 간종괴는 찾을 수 없었으나 수술전 시행한 MRI상 간우정맥우연을 따라 종괴가 2개 관찰되어 간우후구역절 제술을 함께 시행하였다. 수술 후 병리 조직 검사상 간 조직은 염증 소견 보이고 십이지장은 선암 진단 되었다. 최종적으로 십이지장 선암은 pT1bN0M0으로 1기로 진단되어 추가 항암치료 없이 외래 경과 관찰 중이다. 저자들은 전이성 십이지장 내분비암으로 항암치료 받고 완전 관해 되었으나 1년 후 같은 부위에서 선암이 발생하여 수술 시행후 완전 절제 되어 보고하는 바이다.

교통사고에 의해 발생한 뇌내 좌상 1례

박혜연,전소분,김선아,정금선,조종기,송근호,이영원 충남대학교 수의과대학 동물의과학연구소 2004 動物醫科學硏究誌 Vol.12 No.-

A two years old intact female Minature Schnauzer was referred to the Veterinary Medical Teaching Hospital of Chungnam National University with an intermittent seizure by traffic accident. No specific finding was found by radiography and computer tomography (CT). Hematologic and blood chemical examination revealed that PCV (62.5%), BUN (72.6 mg/dl), CK (1769 U/l), ALT (169 U/l) and ALP (353 U/l) were increased. This dog was recovered by medical treatment.

말티즈견에서 발생한 승모판폐쇄부전 1례

전소분,박혜연,김선아,정금선,이영원,박성준,김덕환,송근호 충남대학교 수의과대학 동물의과학연구소 2004 動物醫科學硏究誌 Vol.12 No.-

A 10 years old, intact male, Maltese dog with clinical signs of labored respiration, exercise intolerance and increasing of coughing was referred to the Veterinary Medical Teaching Hospital of Chungnam National University. Physical examination revealed tachycardia, panting and mild cyanosis. This case was diagnosed as mitral valve insufficiency by clinical signs, ascultation, blood chemistry, radiography and electrocardiography. This dog was treated with ACE inhibitor (enalapril), diuretics (furosemide) and bronchodilator (aminophylline) for 14 days. After treatment, clnical signs were disappeared and also this dog was needed to be continuous monitoring and sodium restricted diet.

강도별 운동 전·후 혈청 LDH, 및 CK의 활성도 변화

임용택,송분도,이충영,박명수 한국스포츠리서치 2005 한국 스포츠 리서치 Vol.16 No.2

The purpose of this study was to investigate the changes of Creatine Kinase(CK) and Lactate Dehydrogenase(LDH) activity following different exercise intensities. Each subject(N=G) performed 30min's running with different exercise intensities- 10% VO₂max, 60%VO₂max, 80VO₂max - using treadmill. To measure the blood concentration of CK and LDH, we collected blood three times - pre-exercise, post-exercise and 30min after cessation of exercise. Using this experimental method. following results were obtained; First, There was significant difference between pre- and post-exercise CK concentration in 40%VO₂max exercise group and 60%VO₂max exercise group(p<.05), but no significant difference in 80%VO₂max exercise group. Second, There was no significant difference between pre- and post-exercise LDH concentration in 40%VO₂max exercise group and 60%VO₂max exercise group. but significant difference in 80%VO₂max exercise group. This result suggested that the concentration of CK and LDH following exercise depend on exercise intensities.

The Korean Society of Gastroenterology& SLDDS 2039 : Slide Session ; K-BP-29 : Pancreatobiliary ; Visceral Obesity is Associated with Poor Prognosis in Pancreatic Adenocarcinoma

( Bun Kim ),( Moon Jae Chung ),( Se Woo Park ),( Jeong Youp Park ),( Seung Min Bang ),( Seung Woo Park ),( Si Young Song ),( Jae Bock Chung ) 대한내과학회 2014 대한내과학회 추계학술대회 Vol.2014 No.1

Background: An association between obesity and unfavorable outcomes for various types of malignancy has been established. Nevertheless, the impact of visceral obesity on outcomes in pancreatic cancer is still unknown and controversial. The aim of this study was to uncover an association between visceral obesity (VO) and pancreatic cancer outcomes. Methods: We retrospectively reviewed 499 patients in pancreatic cancer who were diagnosed and treated in the Severance Hospital from January 2006 to December 2011, and collected the data of the clinical features, pathologic fi nding, staging, tumor marker and history of surgery, radiation therapy or chemotherapy. In this study, progression free survival (PFS) and overall survival (OS) were used as factors of cancer prognosis to evaluate association with visceral fat (defi ned as an abdominal fat volume distribution using CT scan obtained from a slice with a disc reference (L3-L4); the percentage of visceral fat to total fat area (VF%) at initial diagnosis. Results: Compared to the low visceral obesity group (low-VO group, n=260), the high visceral obesity group (high-VO group, n=239) was mostly male (68.2% vs. 31.8%, p<0.001), and was more likely to have current smoking status (29.7% vs. 17.7%, p<0.001), current alcohol intake status (52.3% vs. 26.4%, p<0.001) and diabetes mellitus (54.4% vs. 31.9%, p=0.028). The PFS and OS were found to be signifi cantly shorter by the Kaplan-Meier method in the high-VO group than in the low-VO group (PFS: p=0.044, OS: p=0.013). In addition, the higher percentage of visceral fat was correlated with more lymph node metastasis and shorter OS (correlation coeffi cient: 0.165, p=0.011 and -0.107, p=0.017, respectively). Conclusions: In patients with pancreatic cancer, VO at the time of diagnosis is associated with negative outcomes, such as shorter PFS and OS.

CASE REPORT : Duodenal adenocarcinoma following a neuroendocrine tumor in the duodenum

( Bun Kim ),( Ji Hye Huh ),( Young Sook Kim ),( Moon Jae Chung ),( Jeong Youp Park ),( Si Young Song ),( Seung Woo Park ) 대한내과학회 2014 The Korean Journal of Internal Medicine Vol.29 No.1

Primary duodenal adenocarcinoma is a rare malignant neoplasm accounting for 0.3% of all gastrointestinal tract carcinomas. We herein present one case of duode-nal adenocarcinoma after duodenal neuroendocrine carcinoma. Poorly differenti-ated duodenal neuroendocrine carcinoma with liver metastasis (TxNxM1) was confirmed, and eight cycles of palliative chemotherapy (5-fluorouracil/etoposide/cisplatin) were administered. The patient was then in a clinically complete re-sponse status. About 1 year later, newly developed adenocarcinoma was detected at the same site. It was completely surgically resected, and the patient was cured.

Visceral Obesity is Associated with Poor Prognosis in Pancreatic Adenocarcinoma

Kim, Bun,Chung, Moon Jae,Park, Se Woo,Park, Jeong Youp,Bang, Seungmin,Park, Seung Woo,Song, Si Young,Chung, Jae Bock Taylor Francis 2016 Nutrition and cancer Vol.68 No.2

도서지역 여성의 평생교육참여 저해요인 분석

박진영(Park Jin-Young),박분희(Park Bun-Hee) 한국평생교육학회 2005 평생교육학연구 Vol.11 No.3

본 연구는 도서지역 여성을 대상으로 교육참여 저해요인을 분석하고 개인배경에 따라 교육참여 저해요인에 차이가 있는지를 양적조사와 질적조사를 병행하여 분석하였다. 조사대상자는 전남지역 도서에 해당하는 하의도, 하조도, 노화도, 넙도에 거주하는 181명의 여성이다. 연구결과, 고연령층일수록, 학력수준이 무학일수록, 저소득자일수록, 주부들일수록 교육경험이 없다고 응답하였다. 교육참여 저해요인을 분석한 결과는 정보저해, 지역상황저해, 성향저해, 기관저해, 개인상황저해 등 5개의 요인이 도출되었다. 5개 요인중 지역상황저해요인 점수가 가장 높아 그만큼 조사지역에 교육기관의 부재가 심각함을 드러내 주었다. 개인배경별 교육참여 저해요인의 차이를 분석한 결과, 연령이 높을수록, 학력수준이 낮을수록, 소득수준이 낮을수록 지역상황저해요인을 제외하고는 모두 통계적으로 유의미하게 참여 저해점수가 높게 나타났다. 직업에서는 성향저해요인에서만 주부와 농업에 종사하는 여성들의 참여 저해점수가 유의미하게 높았다. This study analyzed barrier factors of educational participation on women who live in island, and tested whether barrier factors make a difference by personal background. And this study performed quantitative and qualitive survey at the same time. The subjects of study are 181 women who live in hai, hajo, nowha, and nup-island in Jeonnam. According to this results, first, there isn't educational experience among women who are more than 60, unschooled, under 10 million won in income, and housewives. Second, extracted barrier factors are informational barrier, situational barrier related region, dispositional barrier, institutional barrier, situational barrier related person. Third, result comparing the mean grades of barrier factors is that the grade of situational barrier related region is highest. Finally, result to analyze differences of barrier factors by personal background is that the grade of the old, the low schooled, the low incomer is high in 4 factors except situational barrier related region. And grades of housewives and women of doing farm work are meaningfully high only in dispositional barrier factor.

Diagnosis of Mycobacterium tuberculosis complex using real-time PCR and melting point analysis with PNA probe

Jiyeong Park(Jiyeong Park),Ha-Young Kim(Ha-Young Kim),Chung Hyun Kim(Chung Hyun Kim),Bun Seung Jo(Bun Seung Jo),Yu-Ran Lee(Yu-Ran Lee),ByungJae So(ByungJae So),Jong Wan Kim(Jong Wan Kim) 한국예방수의학회 2019 한국예방수의학회 학술대회자료집 Vol.2018 No.-