Caffeine Inhibits Cell Proliferation and Regulates PKA/GSK3β Pathways in U87MG Human Glioma Cells

Bo Mi Ku,Yeon Kyung Lee,Joo Yeon Jeong,Jinhyun Ryu,Jungil Choi,Joon Soo Kim,Yong Woon Cho,Gu Seob Roh,Hyun Joon Kim,Gyeong Jae Cho,Wan Sung Choi,Sang Soo Kang 한국분자세포생물학회 2011 Molecules and cells Vol.31 No.3

Caffeine is the most commonly ingested methylxanthine and has anti-cancer effects in several types of cancer. In this study, we examined the anti-cancer effects of caffeine on gliomas, both in vitro and in vivo. In vitro, caffeine treatment reduced glioma cell proliferation through G_0/G_1-phase cell cycle arrest by suppressing Rb phosphorylation. In addition, caffeine induced apoptosis through caspase-3activation and poly(ADP-ribose) polymerase (PARP) cleavage. Caffeine also phosphorylated serine 9 of glycogen synthase kinase 3 beta (GSK3β). Pretreatment with H89, a pharmacological inhibitor of protein kinase A (PKA), was able to antagonize caffeine-induced GSK3β^(ser9) phosphorylation,suggesting that the mechanism might involve a cAMP-dependent PKA-dependent pathway. In vivo, caffeine-treated tumors exhibited reduced proliferation and increased apoptosis compared with vehicle-treated tumors. These results suggest that caffeine induces cell cycle arrest and caspase-dependent cell death in glioma cells,supporting its potential use in chemotherapeutic options for malignant gliomas.

Transglutaminase 2 inhibition found to induce p53 mediated apoptosis in renal cell carcinoma

Ku, Bo Mi,Kim, Dae-Seok,Kim, Kyung-Hee,Yoo, Byong Chul,Kim, Seok-Hyun,Gong, Young-Dae,Kim, Soo-Youl The Federation of American Societies for Experimen 2013 The FASEB Journal Vol.27 No.9

<P>Renal cell carcinoma (RCC), the predominant form of kidney cancer, is characterized by high resistance to radiation and chemotherapy. This study shows that expression of protein cross-linking enzyme transglutaminase 2 (TGase 2) is markedly increased in 7 renal cell carcinoma (RCC) cell lines in comparison to HEK293 and other cancer cell lines, such as NCI 60. However, the key role of TGase 2 in RCC was not clear. The down-regulation of TGase 2 was found to stabilize p53 expression, thereby inducing a 3- to 10-fold increase in apoptosis for 786-O, A498, CAKI-1, and ACHN cell lines by DAPI staining. MEF cells from TGase 2<SUP>−/−</SUP> mice showed stabilized p53 under apoptotic stress to compare to MEFs from wild-type mice. TGase 2 directly cross links the DNA binding domain of p53, leading to p53 depletion <I>via</I> autophagy in RCC. TGase 2 and p53 expression showed an inverse relationship in RCC cells. This finding implies that induced expression of TGase 2 promotes tumor cell survival through p53 depletion in RCC.—Ku, B.M., Kim, D.-S. Kim, K.-H., Yoo, B.C., Kim, S.-H., Gong, Y.-D., Kim, S.-Y. Transglutaminase 2 inhibition found to induce p53 mediated apoptosis in renal cell carcinoma.</P>

$PKC{\eta}$ Regulates the $TGF{\beta}3$-induced Chondrogenic Differentiation of Human Mesenchymal Stem Cell

Ku, Bo Mi,Yune, Young Phil,Lee, Eun Shin,Hah, Young-Sool,Park, Jae Yong,Jeong, Joo Yeon,Lee, Dong Hoon,Cho, Gyeong Jae,Choi, Wan Sung,Kang, Sang Soo The Korean Society of Developmental Biology 2013 발생과 생식 Vol.17 No.4

Transforming growth factor (TGF) family is well known to induce the chondrogenic differentiation of mesenchymal stem cells (MSC). However, the precise signal transduction pathways and underlying factors are not well known. Thus the present study aims to evaluate the possible role of C2 domain in the chondrogenic differentiation of human mesenchymal stem cells. To this end, 145 C2 domains in the adenovirus were individually transfected to hMSC, and morphological changes were examined. Among 145 C2 domains, C2 domain of protein kinase C eta ($PKC{\eta}$) was selected as a possible chondrogenic differentiation factor for hMSC. To confirm this possibility, we treated $TGF{\beta}3$, a well known chondrogenic differentiation factor of hMSC, and examined the increased-expression of glycosaminoglycan (GAG), collagen type II (COL II) as well as $PKC{\eta}$ using PT-PCR, immunocytochemistry and Western blot analysis. To further evaluation of C2 domain of $PKC{\eta}$, we examined morphological changes, expressions of GAG and COL II after transfection of $PKC{\eta}$-C2 domain in hMSC. Overexpression of $PKC{\eta}$-C2 domain induced morphological change and increased GAG and COL II expressions. The present results demonstrate that $PKC{\eta}$ involves in the TGF-${\beta}3$-induced chondrogenic differentiation of hMSC, and C2 domain of $PKC{\eta}$ has important role in this process.

Caffeine inhibits cell proliferation and regulates PKA/GSK3β pathways in U87MG human glioma cells

Ku, Bo Mi,Lee, Yeon Kyung,Jeong, Joo Yeon,Ryu, Jinhyun,Choi, Jungil,Kim, Joon Soo,Cho, Yong Woon,Roh, Gu Seob,Kim, Hyun Joon,Cho, Gyeong Jae,Choi, Wan Sung,Kang, Sang Soo Springer-Verlag 2011 Molecules and cells Vol.31 No.3

The Mediating Effect of Hospital Organizational Health on the Relationship between Positive Nursing Organizational Culture, Caring Behaviors, and Quality of Nursing Care

Ku, Bo Ram,Yu Mi 한국간호과학회 2023 한국간호과학회 학술대회 Vol.2023 No.11

[P10-09] Physiological Activities of Fluid and Solid Chungkookjang

Bo-Mi Lee,Seon-Bin Park,Young-Bae Kim,Hyun-Ku Kim 한국식품영양과학회 2006 한국식품영양과학회 학술대회발표집 Vol.2006 No.10

Physiological Activities of Fluid Chungkookjang with Red Ginseng

( Bo Mi Lee ),( Seon Bin Park ),( Young Bae Kim ),( Hyun Ku Kim ) 한국식품저장유통학회 ( 구 한국농산물저장유통학회 ) 2006 한.일 공동 심포지움 Vol.2006 No.1

Poster Session 2 : Plant Biology ; Isolation of tobacco catalase isozyme 1 (salicylic acid binding protein) interacting with 2a polymerase of cucumber mosaic virus in vivo and in vitro

( Bo Mi Joo ),( Hyun Ku Kang ),( Young In Park ) 한국생화학분자생물학회 (구 한국생화학회) 2004 생화학분자생물학회 춘계학술발표논문집 Vol.2004 No.-

반응표면분석을 이용한 생청국장 추출물의 기능성에 대한 모니터링

이보미(Bo?Mi Lee),도정룡(Jeong?Ryong Do),김현구(Hyun?Ku Kim) 한국식품영양과학회 2007 한국식품영양과학회지 Vol.36 No.10

생청국장의 추출공정별 청국장 추출물의 특성 비교 및 청국장의 최적 추출 조건을 설정하고자 하였다. 용매로 물을 사용하여 추출한 생청국장을 각각 마이크로웨이브 파워별(30, 60, 90, 120 및 150 W), 시간별(1, 5, 9, 13 및 17분)로 추출한 후 생리활성 실험을 수행하였을 때, 수율, 전자공여능, 아질산염 소거능, tyrosinase 저해작용, SOD 유사활성등의 생리활성 측정을 통해 추출 최적점을 찾아내었다. 반응표면분석법을 이용하여 모니터링한 결과, 최적의 조건은 수율, 전자공여능, SOD 유사활성이 일치하는 범위로서 마이크로웨이브 파워 75~140 W, 시료에 대한 용매비 0~11 g/mL, 추출시간 3~12 min으로 나타났다. 예측 결과에 의한 최적 조건을 확인하기 위하여 예측된 최적조건 범위 내에서 임의의 조건을 대입하여 이화학적 특성을 예측해본 결과 생청국장에 대한 임의의 조건으로 시료에 대한 파워 90 W, 용매비10 g/mL, 추출시간 9 min을 설정한 후 생청국장에 대한 각각의 회귀식에 대입하여 얻은 예측된 최적값은 수율 8.90%, 전자공여능 6.97%, 아질산염 소거능 13.13%, tyrosinase 저해작용 12.46%, SOD 유사활성 48.47%로 실험값과 예측값간의 유의적인 차이가 나타나지 않았다. Extraction characteristics of solid Cheonggukjang and the functional properties of corresponding extracts were monitored by the response surface methodology (RSM). The maximum extraction yield of 12.08% was obtained under the specific extraction conditions, such as microwave power of 132.40 W, ratio of solvent to sample content of 7.57 g/mL and extraction time of 14.11 min. The maximum electron donating ability (EDA) was found as 33.53% at the conditions of 113.26 W microwave power, 16.39 g/mL of ratio of solvent to sample content and 12.67 min extraction time. The maximum superoxide dismutase (SOD)-like activity was 89.62% under the extraction conditions of 61.82 W, 17.06 g/mL and 9.03 min. Based on superimposition of three dimensional RSM with respect to extraction yield, electron donating ability and SOD-like activity obtained under the various extraction conditions, the optimum ranges of extraction conditions were found to be microwave power of 75~140 W, ratio of solvent to sample content of 3~12 g/mL and extraction time of 1~11 min, respectively.

소아 급성 하기도 감염의 원인 바이러스 및 이의 유행양상

윤보영,이환종,김미란,윤종구 대한감염학회 1995 감염 Vol.27 No.4

목적: croup, 모세기관지염, 기관 기관지염, 및 폐렴 등의 하기도 감염증에 이환된 소아에서 원인 바이러스를 규명함으로써, 우리나라 소아의 하기도 감염증에 있어서 계절, 연령, 임상형 등에 따른 respiratory syncytial virus(RSV), parainfluenza virus type 1, 2, 3, influenza virus A, B 및 adenovirus 등의 각 호흡기 바이러스의 유행 양상을 밝힌다. 이로써 소아 하기도 감염증의 진단, 치료, 예방에 도움을 얻고, 향후 국내 소아의 호흡기 감염증 연구의 방향을 가늠하는 기초 자료로 활용하고자 한다. 방법: 1990년 11월부터 1994년 4월까지 서울대학교 어린이병원에 입원하거나 외래 또는 응급실을 방문한 소아중 croup, 기관 기관지염, 모세기관지염, 폐렴 등의 하기도 감염증으로 진단받은 환아 712명에서 804회에 걸쳐 비흡인물(nasal aspirate)을 채취하여 단일 클론 항체를 이용한 면역 형광 검사법 및 바이러스 배양으로 369예(45.9%)에서 바이러스성 하기도 감염증을 진단하였다. 결과: 1) 바이러스성 하기도 감염증으로 진단된 환아들의 연령 분포는 2중에서 14년 5개월(중앙 연령 9개월)이었고, 남녀비가 1.75:1이었다. 6개월 미만이 36.3%로 가장 많았고, 연령이 증가 할수록 빈도가 감소하는 추세를 보였다. 이들중 선행 질환이 있었던 경우가 170예(46.1%)였다. 2) 369개(45.9%)의 비흡인물에서 381균주의 바이러스가 확인되었다. RSV가 219예(27.2%)로 가장 많이 분리되었고, 그 다음으로 parainfluenza virus type 3가 63예(7.8%)였으며, 그외 influenza A 및 B virus가 42예(5.2%), adenovirus가 31예(3.9%), parainfluenza virus type 1이 14예(1.7%) 등의 순서로 분리되었다. 혼합 감염증은 12예(3.3%)였다. 3) 바이러스성 하기도 감염증으로 진단된 환아들의 임상상은 폐렴(56.6%), 모세기관지염(35.2%), croup(6.5%), 기관 기관지염(1.6%) 등 이었다. 4) RSV, parainfluenza viruses(type 1, 3), influenza viruses(A, B) 등의 감염증은 주로 유행성으로 발생하였으며, adenovirus 감염증은 연중 산발적으로 발생하였다. 42개월의 연구 기간중 RSV는 가장 큰 유행을 일으켰으며, 연구 기간중 4변의 유행 기간이 있었다. 결론: 하기도 감염증을 가진 대상 환아중 45.9%에서 수종의 호흡기 바이러스가 분리되어 바이러스가 우리나라 소아 하기도 감염증의 주요한 원인으로 생각되며, 이중 특히 RSV가 차지하는 비중이 크다. 각 호흡기 바이러스마다 임상 양상이나 유행시기가 다르고 다른 나라의 보고와 차이점이 있는 부분도 있었다. 향후 우리나라에서도 바이러스성 하기도 감염증의 중요성이 인식되고 더욱 많은 연구가 활발히 진행되어 환자 진단, 치료 및 질병 예방에 이용되어야 할 것으로 사료된다. Background:Respiratory tract infection is the leading cause of morbidity and mortality in children. Causes of acute lower respiratory tract infections(ALRI) in infants and children are diverse and include bacteria, mycoplasma, and respiratory viruses. There is, however, a wide geographic variation regarding the relative importance of each agent. It is necessary to identify the etiology and epidemiology of ALRI in each community to employ control measures including vaccine policy. In Korea, there haeve been no published data regarding the the viral etiology and epidemiology of ALRI. Methods:Viral etiologic agents of ALRI were studied from November 1990 through April 1994 in Korean children. 804 nasal aspirates were collected from 712 children, who visited or were admitted to Seoul National University Children's Hospital due to acute LRI, and viral agents were detected by virus isolation and/or antigen detection by indirect immunofluorescent staining. Results:One or more viral agents were identified in 369(45.9%) cases, of which 3.3% were mixed (two viral) infections. The pathogens identified were respiratory syncytial virus(RSV)(27.2%), parainfluenza virus type 3(7.8%), influenza A virus(3.9%), adenovirus(3.9%), parainfluenza virus type 1(1.7%), influenza B virus(1.4%), parainfluenza virus type 2(0.5%), measles virus(0.1%), and others(0.9%). The clinical patterns of viral LRI included pneumonia(56.6%), bronchiolitis(35.2%), croup(6.5%), and tracheobronchitis(1.6%). Infections with RSV, parainfluenza virus type 1 and 3, and influenza A and B virus occurred in epidemics, while adenovirus was isolated sporadically throughout the study period. Conclusion: We studied the etiologic agents and epidemiology of viral ALRI in Korean children, the results of which may be helpful to the clinicians and researchers interested in the control of LRI.