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      • KCI등재

        Development and standardization of a monoclonal antibody-based rapid flow-through immunoassay for the detection of Aphanomyces invadans in the field

        B. Adil,K. M. Shankar,B. T. Naveen Kumar,Rajreddy Patil,Abhiman Ballyaya,K. S. Ramesh,Sathish Rama Poojary,Omkar V. Byadgi,Prabhugouda Siriyappagouder 대한수의학회 2013 Journal of Veterinary Science Vol.14 No.4

        A monoclonal antibody-based flow-through immunoassay (FTA) was developed using a nitrocellulose membrane placed on the top of adsorbent pads enclosed in a plastic cassette with a test zone at the center. The FTA could be completed within 10 min. Clear purple dots against a white background indicated the presence of Aphanomyces (A.)invadans. The FTA limit of detection was 7 μg/mL for A. invadans compared to 56 μg/mL for the immunodot. FTA and polymerase chain reaction (PCR) could detect A. invadans in fish tissue homogenates at a 10-11 dilution compared to a 10-8 dilution by immunodot. In fish suffering from natural cases of epizootic ulcerative syndrome (EUS)collected from Mangalore, India, FTA and PCR could detect A. invadans in 100% of the samples compared to 89.04%detected by immunodot. FTA reagents were stable and produced expected results for 4 months when stored at 4∼8oC. This rapid test could serve as simple and cost-effective on-site screening tool to detect A. invadans in fish from EUS outbreak areas and in ports during the shipment of live or frozen fish.

      • KCI등재

        Structural performance of cold-formed steel composite beams

        M. Adil Dar,N. Subramanian,M. Anbarasu,A.R. Dar,James B.P. Lim 국제구조공학회 2018 Steel and Composite Structures, An International J Vol.27 No.5

        This study presents a novel method of improving the strength and stiffness of cold-formed steel (CFS) beams. Flexural members are primary members in most of the structures. Hence, there is an urgent need in the CFS industry to look beyond the conventional CFS beam sections and develop novel techniques to address the severe local buckling problems that exist in CFS flexural members. The primary objective of this study was to develop new CFS composite beam sections with improved structural performance and economy. This paper presents an experimental study conducted on different CFS composite beams with simply supported end conditions under four point loading. Material properties and geometric imperfections of the models were measured. The test strengths of the models are compared with the design strengths predicted by using Australian/New Zealand Standard for cold-formed steel structures. Furthermore, to ensure high precision testing, a special testing rig was also developed for testing of long span beams. The description of test models, testing rig features and test results are presented here. For better interpretation of results, a comparison of the test results with a hot rolled section is also presented. The test results have shown that the proposed CFS composite beams are promising both in terms of better structural performance as well as economy.

      • KCI등재

        Flexural Strength of cold-formed steel built-up composite beams with rectangular compression flanges

        M. Adil Dar,N. Subramanian,Dawood A. Dar,A.R. Dar,M. Anbarasu,James B. P. Lim,Soroush Mahjoubi 국제구조공학회 2020 Steel and Composite Structures, An International J Vol.34 No.2

        The past research on cold-formed steel (CFS) flexural members have proved that rectangular hollow flanged sections perform better than conventional I-sections due to their higher torsional rigidity over the later ones. However, CFS members are vulnerable to local buckling, substantially due to their thin-walled features. The use of packing, such as firmly connected timber planks, to the flanges of conventional CFS lipped I-sections can drastically improve their flexural performance as well as structural efficiency. Whilst several CFS composites have been developed so far, only limited packing materials have been tried. This paper presents a series of tests carried out on different rectangular hollow compression flanged sections with innovative packing materials. Four-point flexural tests were carried out to assess the flexural capacity, failure modes and deformed shapes of the CFS composite beam specimens. The geometric imperfections were measured and reported. The North American Specifications and Indian Standard for cold-formed steel structures were used to compare the design strengths of the experimental specimen. The test results indicate clearly that CFS rectangular ‘compression’ flanged composite beams perform significantly better than the conventional rectangular hollow flanged CFS sections.

      • KCI등재

        Effect of angle stiffeners on the flexural strength and stiffness of cold-formed steel beams

        M. Adil Dar,N. Subramanian,Amer I. Rather,A.R. Dar,James B. P. Lim,M. Anbarasu,Krishanu Roy 국제구조공학회 2019 Steel and Composite Structures, An International J Vol.33 No.2

        Cold-formed steel (CFS) sections when used as primary load carrying members often require additional strengthening for retrofitting purposes. In some cases, it is also necessary to reduce deflections in order to satisfy serviceability requirements. The introduction of angle sections, screwed to the webs so as to act as external stiffeners, has the potential to both increase flexural strength as well as reduce deflections. This paper presents the results of ten four-point bending tests, on built-up CFS sections, both open and closed, with different stiffening arrangements. In the laboratory tests, the stiffening arrangements increased the moment capacity and stiffness of the CFS beams by up to 85% and 100% respectively. The increase in moment capacity was more evident for the open sections, while that reduction in deflection was largest for the closed sections.

      • KCI등재

        Efficient cross-sectional profiling of built up CFS beams for improved flexural performance

        M. Adil Dar,N. Subramanian,Mir Atif,A.R. Dar,M. Anbarasu,James B.P. Lim 국제구조공학회 2020 Steel and Composite Structures, An International J Vol.34 No.3

        In the past, many efficient profiles have been developed for cold-formed steel (CFS) members by judicious intermediate stiffening of the cross-sections, and they have shown improved structural performance over conventional CFS sections. Most of this research work was based on numerical modelling, thus lacking any experimental evidence of the efficiency of these sections. To fulfill this requirement, experimental studies were conducted in this study, on efficient intermediately stiffened CFS sections in flexure, which will result in easy and simple fabrication. Two series of built-up sections, open sections (OS) and box sections (BS), were fabricated and tested under four-point loading with same cross-sectional area. Test strengths, modes of failure, deformed shapes, load vs. mid-span displacements and geometric imperfections were measured and reported. The design strengths were quantified using North American Standards and Indian Standards for cold-formed steel structures. This study confirmed that efficient profiling of CFS sections can improve both the strength and stiffness performance by up to 90%. Closed sections showed better strength performance whereas open sections showed better stiffness performance.

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