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Early Disease Detection of Bacterial Leaf Blight on Rice Plant by using Hyperspectral Imaging
( M Naufal Rauf Ibrahim ),( Takashi Okayasu ),( Atsushi Yoshimura ),( Yoshiyuki Yamagata ),( Naruto Furuya ),( Eiji Inoue ),( Yasumaru Hirai ),( Muneshi Mitsuoka ) 한국농업기계학회 2018 한국농업기계학회 학술발표논문집 Vol.23 No.1
In Southeast Asia, bacterial leaf blight (BLB) is one of the significant rice diseases that potentially causes productivity loss up to 50%. The early stage disease detection contributes to prevent the serious crop damage due to the disease outbreak. The hyperspectral imaging provides an extension of our vision by capturing the near infrared (NIR) region at 700-1000 nm which is the outside of visible light (VIS) region at 400-700 nm. In this study, the possibility of early stage disease detection method of BLB on rice plant was investigated in captured hyperspectral images with 40-50 cm from the top of rice plant. For early disease setting, the hyperspectral images for both the healthy and infected single leaf of the rice plants, both were taken from 2 days post-inoculation when the symptom had not been shown yet. The possible sensitive bands were explored from VIS to NIR and chosen by applying the principal component analysis (PCA) on reflectance value of the healthy and infected leaves using 400-850 nm. The PCA result provided that there several ranges of wavelength have high correlation with the disease development on early stage and found that the ratio of orange-red color (605-640 nm) and red edge (690-720 nm) has the highest possibility to detect the BLB.
Baculovirus-mediated gene transfer systems in silkworm larvae using constitutive host promoters
Jae Man Lee,Jian Xu,Hiroaki Mon,TakumiMitsudome,Atsushi Masuda,Kaito Yoshimura,Kazuhiro Iiyama,Yuuka Chieda,Takahiro Kusakabe 한국응용곤충학회 2014 Journal of Asia-Pacific Entomology Vol.17 No.1
Baculoviruses serve as efficient viral vectors for gene delivery into vertebrate and invertebrate cells. The identificationand characterization of the functional promoters in different baculovirus-infected hosts are essentialfor the efficient gene expression. To establish a baculovirus-mediated gene transfer system in the silkworm,Bombyxmori, we investigated the activities of silkworm-derived TCTP, ACTIN3, and HSC70-4 promoters deliveredby AcNPV or BmNPV in various tissues of silkworm. In many of the tested silkworm tissues, the BmHSC70-4promoter exhibited a higher transcription activity than those of BmTCTP or BmACTIN3 promoters when deliveredby AcNPV, which is reported to be incapable of replicating in silkworms. In contrast, the BmACTIN3 promoter wasfound to be the strongest promoterswhen delivered by BmNPV. The present results indicate that the BmHSC70-4promoter is potentially useful for the stable gene expression by the non-replicating AcNPV vector for gene functionanalysis in the silkworm.
Takeshi Ueda,Tetsuya Tanaka,Yuki Kirihataya,Chisato Hara,Atsushi Yoshimura 대한내시경로봇외과학회 2023 Journal of Minimally Invasive Surgery Vol.26 No.4
Left-sided gallbladder is a rare finding that is mostly discovered incidentally during surgery and is often associated with anatomic anomalies. We herein report a case in which laparoscopic cholecystectomy and common bile duct exploration were achieved for an 89-year-old female patient with left-sided gallbladder. Surgery was carried out using our usual trocar position. Calot triangle was covered by the body of the gallbladder and could not be detected. We dissected the gallbladder from the fundus towards the neck. The cystic duct joined the common bile duct from the right side, and common bile duct exploration was performed routinely without perioperative comorbidities. Although the preoperative diagnosis rate is low and the risk of intraoperative bile duct injuries in patients with left-sided gallbladder is high, laparoscopic cholecystectomy and common bile duct exploration can be safely performed by understanding the location and bifurcation of the cystic duct.