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      • Early Disease Detection of Bacterial Leaf Blight on Rice Plant by using Hyperspectral Imaging

        ( M Naufal Rauf Ibrahim ),( Takashi Okayasu ),( Atsushi Yoshimura ),( Yoshiyuki Yamagata ),( Naruto Furuya ),( Eiji Inoue ),( Yasumaru Hirai ),( Muneshi Mitsuoka ) 한국농업기계학회 2018 한국농업기계학회 학술발표논문집 Vol.23 No.1

        In Southeast Asia, bacterial leaf blight (BLB) is one of the significant rice diseases that potentially causes productivity loss up to 50%. The early stage disease detection contributes to prevent the serious crop damage due to the disease outbreak. The hyperspectral imaging provides an extension of our vision by capturing the near infrared (NIR) region at 700-1000 nm which is the outside of visible light (VIS) region at 400-700 nm. In this study, the possibility of early stage disease detection method of BLB on rice plant was investigated in captured hyperspectral images with 40-50 cm from the top of rice plant. For early disease setting, the hyperspectral images for both the healthy and infected single leaf of the rice plants, both were taken from 2 days post-inoculation when the symptom had not been shown yet. The possible sensitive bands were explored from VIS to NIR and chosen by applying the principal component analysis (PCA) on reflectance value of the healthy and infected leaves using 400-850 nm. The PCA result provided that there several ranges of wavelength have high correlation with the disease development on early stage and found that the ratio of orange-red color (605-640 nm) and red edge (690-720 nm) has the highest possibility to detect the BLB.

      • 2015 Nationwide Survey Revealed Barley stripe mosaic virus in Korean Barley Fields

        Lim, Hyo-Jin,Seo, Eun-Young,Kim, Hyun-Seung,Kim, Jung-Kyu,Park, Chan-Hwan,Gong, Jun-Su,Kim, Ik-hyun,Han, Sang-huk,Kilcrease, James P.,Furuya, Naruto FACULTY OF AGRIC PUBLICATIONS-KYUSHU UNIV 2016 Journal of the Faculty of Agriculture, Kyushu Univ Vol.61 No.1

        <P>A seed transmitted virus has consistently caused significant economic damage to barley crops in Korea in recent years, and may be increasing because many farmers save seed for replanting. Because some barley seed is imported, there is the potential for introduction of new seed transmitted viruses, causing diseases which may spread. Barley cultivation in South Korea is expanding nationwide due to the increasing popularity of health foods, so both production and quality of barley grain is important. Although Barley stripe mosaic virus (BSMV) has been reported previously as a small percentage of all barley viruses present in Korea, increases in imports of barley seed may lead to increased occurrence of this seed transmitted virus. We therefore investigated the virus status of barley crops around Iksan and Wanju. On several newly cultivated barley farms, we observed areas showing symptoms typical of BSMV, and confirmed BSMV infection in 24% of the samples examined. In order to understand the occurrence and seed transmission of Korean BSMV isolates, we examined sequence variation within the Triple Gene Block proteins, and subcellular localization of two of these proteins. The newly identified Korean BSMV isolates show low sequence variability and high sequence homology to previously reported US isolates. With these results, we expect to confirm distribution of barley viruses and possible emerging viruses, which will serve as base line data to document virus prevention and control measures.</P>

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