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Atsushi Masuda,Minoru Akitomi,Masanao Inoue,Keizo Okuwaki,Atsuo Okugawa,Kiyoshi Ueno,Toshiharu Yamazaki,Kohjiro Hara 한국물리학회 2016 Current Applied Physics Vol.16 No.12
Processes for potential-induced degradation (PID) and recovery phenomena were characterized using ptype multicrystalline Si photovoltaic modules and by PID test method using Al plate. Very severe PID phenomena accompanied with a drastic reduction in both open-circuit voltage and shunt resistance were observed within only several hours. It was found that PID phenomena are strongly accelerated at higher temperature and under higher negative-voltage application, on the other hand, PID phenomena do not necessarily require high humidity in this test method using Al plate. Na diffusion from the cover glass to the Si cell was observed after PID test. Recovery process from PID was also observed by applying positive voltage. However, complete recovery of photovoltaic performances was observed at room temperature in the dark without positive-voltage application for test modules with PID although recovery process requires a few hundred days.
Atsushi Masuda,Jian Xu,TakumiMitsudome,Daisuke Morokuma,Hiroaki Mon,Yutaka Banno,Takahiro Kusakabe,이재만 한국응용곤충학회 2015 Journal of Asia-Pacific Entomology Vol.18 No.2
Endo-β-N-acetylglucosaminidase H (Endo H) catalyzes cleavage between the GlcNAc residues of the chitobiose core of N-linked glycans, leaving one GlcNAc residues attached to asparagine. Endo H cleaves high mannose and hybrid, but not complex, N-linked oligosaccharides on glycoproteins. Because of its unique specificity, Endo H is widely used for the structural and functional analyses of glycoproteins. In our previous study, the recombinant Endo H was produced as a secreted protein using silkworm–baculovirus expression system, but the yield was low (30 μg Endo H/10 ml larval hemolymph) compared to that of Escherichia coli. In this study, we purified active recombinant Endo H as an intracellular protein from fat body of silkworm infected with the recombinant baculovirus expressing Endo H without the exogenous signal peptide. Remarkably, the yield (9.3mgfrom20 silkwormlarvae)was about 310-fold higher than that secreted into larval hemolymph as reported previously. In addition,we screened the silkwormstrains maintained in Kyushu University and identified n17 as a high-level expression strain for Endo H.
RUNX1–Survivin Axis Is a Novel Therapeutic Target for Malignant Rhabdoid Tumors
Masamitsu Mikami,Tatsuya Masuda,Takuya Kanatani,Katsutsugu Umeda,Hidefumi Hiramatsu,Hirohito Kubota,Tomoo Daifu,Atsushi Iwai,Etsuko Yamamoto Hattori,Kana Furuichi,Saho Takasaki,Sunao Tanaka,Yasuzumi M 한국분자세포생물학회 2022 Molecules and cells Vol.45 No.12
Malignant rhabdoid tumor (MRT) is a highly aggressive pediatric malignancy with no effective therapy. Therefore, it is necessary to identify a target for the development ofnovel molecule-targeting therapeutic agents. In this study, we report the importance of the runt-related transcription factor 1 (RUNX1) and RUNX1–Baculoviral IAP (inhibitor of apoptosis) Repeat-Containing 5 (BIRC5/survivin) axis in the proliferation of MRT cells, as it can be used as an ideal target for anti-tumor strategies. The mechanism of this reaction can be explained by the interaction of RUNX1 with the RUNX1-binding DNA sequence located in the survivin promoter and its positive regulation. Specific knockdown of RUNX1 led to decreased expression of survivin, which subsequently suppressed the proliferation of MRT cells in vitro and in vivo. We also found that our novel RUNX inhibitor, Chb-M, which switches off RUNX1 using alkylating agent-conjugated pyrrole-imidazole polyamides designed to specifically bind to consensus RUNX-binding sequences (5′-TGTGGT-3′), inhibited survivin expression in vivo. Taken together, we identified a novel interaction between RUNX1 and survivin in MRT. Therefore the negative regulation of RUNX1 activity may be a novel strategy for MRT treatment
Baculovirus-mediated gene transfer systems in silkworm larvae using constitutive host promoters
Jae Man Lee,Jian Xu,Hiroaki Mon,TakumiMitsudome,Atsushi Masuda,Kaito Yoshimura,Kazuhiro Iiyama,Yuuka Chieda,Takahiro Kusakabe 한국응용곤충학회 2014 Journal of Asia-Pacific Entomology Vol.17 No.1
Baculoviruses serve as efficient viral vectors for gene delivery into vertebrate and invertebrate cells. The identificationand characterization of the functional promoters in different baculovirus-infected hosts are essentialfor the efficient gene expression. To establish a baculovirus-mediated gene transfer system in the silkworm,Bombyxmori, we investigated the activities of silkworm-derived TCTP, ACTIN3, and HSC70-4 promoters deliveredby AcNPV or BmNPV in various tissues of silkworm. In many of the tested silkworm tissues, the BmHSC70-4promoter exhibited a higher transcription activity than those of BmTCTP or BmACTIN3 promoters when deliveredby AcNPV, which is reported to be incapable of replicating in silkworms. In contrast, the BmACTIN3 promoter wasfound to be the strongest promoterswhen delivered by BmNPV. The present results indicate that the BmHSC70-4promoter is potentially useful for the stable gene expression by the non-replicating AcNPV vector for gene functionanalysis in the silkworm.
The Development of the Totalized Hydrogen Energy Utilization System for Commercial Buildings
Maeda, Tetsuhiko,Nakano, Akihiro,Ito, Hiroshi,Tange, Manabu,Kawakami, Yoshiaki,Kato, Atsushi,Masuda, Masao The Korean Institute of Electrical Engineers 2011 The Journal of International Council on Electrical Vol.1 No.2
We have proposed the Totalized Hydrogen Energy Utilization System(THEUS) for applying to commercial buildings. THEUS consists of fuel cells, water electrolyzers, metal hydride tanks and their auxiliaries. We evaluated the energy saving of the basic THEUS in view of an actual operation using thermal demand data of actual buildings. The THEUS have ability for achieving energy saving, in comparison with an ice storage system for cooling demand. We have developed the hydrogen storage system using metal hydride and unitized reversible cell (URC) combine the functionality of a fuel cell and an electrolyzer in a single device, these are important components of THEUS. The metal hydride tank designed and manufactured to evaluate that we assumed actual operation in the experiments. We developed the numerical simulation code, and simulation results are in good agreement with experimental data. The simulation about two tanks system was done by using this code. The heat utilization can be improved greatly. To achieve URC commercial use, improvement of durability was examined. It confirmed that our URC had durability for several thousand hours.