Niacinamide Protects Skin Cells from Oxidative Stress Induced by Particulate Matter

( Ao Xuan Zhen ),( Mei Jing Piao ),( Kyoung Ah Kang ),( Pincha Devage Sameera Madushan Fernando ),( Hee Kyoung Kang ),( Young Sang Koh ),( Joo Mi Yi ),( Jin Won Hyun ) 한국응용약물학회 2019 Biomolecules & Therapeutics(구 응용약물학회지) Vol.27 No.6

Niacinamide (NIA) is a water-soluble vitamin that is widely used in the treatment of skin diseases. Moreover, NIA displays antioxidant effects and helps repair damaged DNA. Recent studies showed that particulate matter 2.5 (PM_2.5) induced reactive oxygen species (ROS), causing disruption of DNA, lipids, and protein, mitochondrial depolarization, and apoptosis of skin keratinocytes. Here, we investigated the protective effects of NIA on PM_2.5-induced oxidative stress in human HaCaT keratinocytes. We found that NIA could inhibit the ROS generation induced by PM_2.5, as well block the PM_2.5-induced oxidation of molecules, such as lipids, proteins, and DNA. Furthermore, NIA alleviated PM_2.5-induced accumulation of cellular Ca²⁺, which caused cell membrane depolarization and apoptosis, and reduced the number of apoptotic cells. Collectively, the findings show that NIA can protect keratinocytes from PM_2.5-induced oxidative stress and cell damage.

다적층 활성층 구조 기반의 IZO 박막 트랜지스터에 대한 포괄적인 전기적 분석

Ao Zitong,채성원(Seong-won Chae),문현수(Hyun-soo Moon),김한상(Han-Sang Kim),이재윤(Jae-Yun Lee),Shan Fei,Guo Hongbo,Sun Haozhou,Tukhtaev Anvar,Buranov Ruslan,Jaynarov Sherali,김성진(Sung-Jin Kim) 한국통신학회 2019 한국통신학회 학술대회논문집 Vol.2019 No.1

Purpurogallin Protects Keratinocytes from Damage and Apoptosis Induced by Ultraviolet B Radiation and Particulate Matter 2.5

( Ao Xuan Zhen ),( Mei Jing Piao ),( Yu Jae Hyun ),( Kyoung Ah Kang ),( Yea Seong Ryu ),( Suk Ju Cho ),( Hee Kyoung Kang ),( Young Sang Koh ),( Mee Jung Ahn ),( Tae Hoon Kim ),( Jin Won Hyun ) 한국응용약물학회 2019 Biomolecules & Therapeutics(구 응용약물학회지) Vol.27 No.4

Purpurogallin, a natural phenol obtained from oak nutgalls, has been shown to possess antioxidant, anticancer, and anti-inflammatory effects. Recently, in addition to ultraviolet B (UVB) radiation that induces cell apoptosis via oxidative stress, particulate matter 2.5 (PM_2.5) was shown to trigger excessive production of reactive oxygen species. In this study, we observed that UVB radiation and PM_2.5 severely damaged human HaCaT keratinocytes, disrupting cellular DNA, lipids, and proteins and causing mitochondrial depolarization. Purpurogallin protected HaCaT cells from apoptosis induced by UVB radiation and/or PM_2.5. Furthermore, purpurogallin effectively modulates the pro-apoptotic and anti-apoptotic proteins under UVB irradiation via caspase signaling pathways. Additionally, purpurogallin reduced apoptosis via MAPK signaling pathways, as demonstrated using MAPK-p38, ERK, and JNK inhibitors. These results indicate that purpurogallin possesses antioxidant effects and protects cells from damage and apoptosis induced by UVB radiation and PM_2.5.

Ultimate torsional strength of cracked stiffened box girders with a large deck opening

Ao, Lei,Wang, De-Yu The Society of Naval Architects of Korea 2016 International Journal of Naval Architecture and Oc Vol.8 No.4

The present paper studies the ultimate torsional strength of stiffened box girders with large deck opening due to the influence of cracks. Three types of hull girders with different spans are provided for comparison. Potential parameters which may have effects on the torsional strength including the mesh refinement, initial deflection, material strain hardening, geometric properties of crack and stiffener are discussed. Two new concepts that play an significant role in the ultimate strength research of damaged box girders are introduced, one of which is the effective residual section (ERS), the other is the initial damage of the failure zone (IDFZ) for intact structures. New simple formulas for predicting the residual ultimate torsional strength of cracked stiffened box girders are derived on the basis of the two new concepts.

IL-17A exacerbates diabetic retinopathy by impairing Müller cell function via Act1 signaling

Ao-Wang Qiu,Zheng Bian,Ping-An Mao,Qing-Huai Liu 생화학분자생물학회 2016 Experimental and molecular medicine Vol.48 No.-

Diabetic retinopathy (DR), one of the most serious complications of diabetes, has been associated with inflammatory processes. We have recently reported that interleukin (IL)-17A, a proinflammatory cytokine, is increased in the plasma of diabetic patients. Further investigation is required to clarify the role of IL-17A in DR. Ins2Akita (Akita) diabetic mice and high-glucose (HG)-treated primary Müller cells were used to mimic DR-like pathology. Diabetes induced retinal expression of IL-17A and IL-17 receptor A (IL-17RA) in Müller cells in contrast to ganglion cells. Further evidence demonstrated that retinal Müller cells cultured in vitro increased IL-17A and IL-17RA expression as well as IL-17A secretion in the HG condition. In both the HG-treated Müller cells and Akita mouse retina, the Act1/TRAF6/IKK/NF-κB signaling pathway was activated. IL-17A further enhanced inflammatory signaling activation, whereas Act1 knockdown or IKK inhibition blocked the downstream signaling activation by IL-17A. HG- and diabetesinduced Müller cell activation and dysfunction, as determined by increased glial fibrillary acidic protein, vascular endothelial growth factor and glutamate levels and decreased glutamine synthetase and excitatory amino acid transporter-1 expression, were exacerbated by IL-17A; however, they were alleviated by Act1 knockdown or IKK inhibition. In addition, IL-17A intravitreal injection aggravated diabetes-induced retinal vascular leukostasis, vascular leakage and ganglion cell apoptosis, whereas Act1 silencing or anti-IL-17A monoclonal antibody ameliorated the retinal vascular damage and neuronal cell apoptosis. These findings establish that IL-17A xacerbates DR-like pathology by the promotion of Müller cell functional impairment via Act1 signaling.

Effects of Fermented Garlic Powder on Production Performance, Egg Quality, Blood Profiles and Fatty Acids Composition of Egg Yolk in Laying Hens

Ao, X.,Yoo, J.S.,Lee, J.H.,Jang, H.D.,Wang, J.P.,Zhou, T.X.,Kim, I.H. Asian Australasian Association of Animal Productio 2010 Animal Bioscience Vol.23 No.6

The effects of fermented garlic powder on production performance, egg quality, blood profiles and fatty acid composition of egg yolk in laying hens were studied in a 35-d experiment. Two-hundred and forty (ISA brown) layers aged 41 weeks were randomly allocated into the following four treatments: i) CON (basal diet); ii) G1 (CON+fermented garlic powder 1.0%); iii) G2 (CON+fermented garlic powder 2.0%) and iv) G3 (CON+fermented garlic powder 3.0%). There were no differences (p>0.05) among treatments in egg production, egg weight, eggshell breaking strength and eggshell thickness throughout the whole experimental period. However, yolk height was increased significantly (p<0.05) by the addition of fermented garlic powder during the 5th week while yolk color was greater (p<0.05) in G2 and G3 than in CON and G1 in the 5th week. Compared with CON, Haugh unit was increased (p<0.05) in response to fermented garlic powder treatments during the 5th week. No significant effects on total protein, albumin and IgG were observed in response to any of the treatments over the experimental period (p>0.05). There was a significant (p<0.05) reduction in plasma cholesterol concentration when the dietary level of fermented garlic powder was increased from 0.0 to 3.0%. The levels of saturated fatty acids (SFA) were significantly decreased (p<0.05) in response to G2 and G3 while monounsaturated fatty acids (MUFA) were higher (p<0.05) in G2 and G3 treatment groups than in CON and G1. Compared with other treatments, polyunsaturated fatty acids (PUFA) and PUFA:SFA ratio were higher (p<0.05) in G3. In conclusion, this study demonstrated that addition of fermented garlic powder reduced plasma cholesterol concentration and did not cause adverse effects on production performance. Moreover, addition of 3.0% garlic powder decreased SFA but increased PUFA and PUFA:SFA ratio in egg yolk.

Effects of Dietary Additives and Early Feeding on Performance, Gut Development and Immune Status of Broiler Chickens Challenged with Clostridium perfringens

Ao, Z.,Kocher, A.,Choct, M. Asian Australasian Association of Animal Productio 2012 Animal Bioscience Vol.25 No.4

The effects of dietary additives and holding time on resistance and resilience of broiler chickens to Clostridium perfringens challenge were investigated by offering four dietary treatments. These were a negative control (basal), a positive control (Zn-bacitracin) and two dietary additives, mannanoligosaccharides (MOS), and acidifier. Two holding times included (a) immediate access to feed and water post hatch (FED) and (b) access to both feed and water 48 h post hatch (HELD). Chicks fed Zn-bacitracin had no intestinal lesions attributed to necrotic enteritis (NE), whereas chicks fed both MOS or acidifier showed signs of NE related lesions. All dietary treatments were effective in reducing the numbers of C. perfringens in the ileum post challenge. The FED chicks had heavier body weight and numerically lower mortality. The FED chicks also showed stronger immune responses to NE challenge, showing enhanced (p<0.05) proliferation of T-cells. Early feeding of the MOS supplemented diet increased (p<0.05) IL-6 production. The relative bursa weight of the FED chicks was heavier at d 21 (p<0.05). All the additives increased the relative spleen weight of the HELD chicks at d 14 (p<0.05). The FED chicks had increased villus height and reduced crypt depth, and hence an increased villus/crypt ratio, especially in the jejunum at d 14 (p<0.05). The same was true for the HELD chicks given dietary additives (p<0.05). It may be concluded that the chicks with early access to dietary additives showed enhanced immune response and gut development, under C. perfringens challenge. The findings of this study shed light on managerial and nutritional strategies that could be used to prevent NE in the broiler industry without the use of in-feed antibiotics.

Influence of Fermented Red Ginseng Extract on Broilers and Laying Hens

Ao, X.,Zhou, T.X.,Kim, H.J.,Hong, S.M.,Kim, I.H. Asian Australasian Association of Animal Productio 2011 Animal Bioscience Vol.24 No.7

The aim of this study was to evaluate the potential of fermented red ginseng extract (FRGE) as feed additive in broilers and laying hens. In broilers, 480 Arbor Acre male broilers were randomly allotted to 4 treatments with 6 replications per treatment and 20 chicks per pen. The experiment lasted 5 weeks and dietary treatments were as follows: i) CON, basal diet; ii) FRGE1, basal diet+1 g/kg fermented red ginseng extract; iii) FRGE2, basal diet+2 g/kg fermented red ginseng extract and iv) FRGE3 basal diet+4 g/kg fermented red ginseng extract. Throughout the experiment, no effects were observed (p>0.05) in performance in response to FRGE. At the end of the experiment, FRGE administration improved (p<0.05) the lymphocyte level compared with CON. The relative weight of bursa of fabricius and spleen were increased (p<0.05) by the inclusion of FRGE3. Besides, redness ($a^*$) value for the breast meat was higher (p<0.05) in FRGE1 and FRGE3 treatments than that in CON. In laying hens, 240 ISA brown layers at 35 weeks of age were used in this 8-week trial. Dietary treatments were the same as in the broilers trial with 10 replicates per treatment and 6 layers per replicate. During the entire experiment, there were no significant differences (p>0.05) in performance or egg quality among all the treatments. However, the layers fed diets supplemented with FRGE had higher lymphocyte level (p<0.05) compared with those fed CON. In conclusion, the dietary supplementation with FRGE did not influence performance but improved the lymphocyte level in both broilers and laying hens.

EKF-based Carrier Tracking in One-bit Quantized GNSS Receiver

Ao Peng,Gang Ou,Jianghong Shi 보안공학연구지원센터(IJSIP) 2014 International Journal of Signal Processing, Image Vol.7 No.6

EXPLORING THE FUEL ECONOMY POTENTIAL OF ISG HYBRID ELECTRIC VEHICLES THROUGH DYNAMIC PROGRAMMING

Ao, G.Q.,Qiang, J.X.,Zhong, H.,Yang, L.,Zhuo, B. The Korean Society of Automotive Engineers 2007 International journal of automotive technology Vol.8 No.6

Hybrid electric vehicles(HEV) combined with more than one power sources have great potential to improve fuel economy and reduce pollutant emissions. The Integrated Starter Generator(ISG) HEV researched in this paper is a two energy sources vehicle, with a conventional internal combustion engine(ICE) and an energy storage system(batteries). In order to investigate the potential of diesel engine hybrid electric vehicles in fuel economy improvement and emissions reduction, a Dynamic Programming(DP) based supervisory controller is developed to allocate the power requirement between ICE and batteries with the objective of minimizing a weighted cost function over given drive cycles. A fuel-economy-only case and a fuel & emissions case can be achieved by changing specific weighting factors. The simulation results of the fuel-economy-only case show that there is a 45.1% fuel saving potential for this ISG HEV compared to a conventional transit bus. The test results present a 39.6% improvement in fuel economy which validates the simulation results. Compared to the fuel-economy-only case, the fuel & emissions case further reduces the pollutant emissions at a cost of 3.2% and 4.5% of fuel consumption with respect to the simulation and test result respectively.