Change in pediatric trauma-related visits in a tertiary hospital in Colombia during coronavirus disease 2019 lockdown

Adriana Cuenca,Aura Coy,Natalia Gutiérrez,María Paula Santos,Juan David Bustos,Ana María Morales,Alejandra Marín 대한소아응급의학회 2023 대한소아응급의학회지 Vol.10 No.1

Purpose: During the coronavirus disease 2019 pandemic, Colombian government declared a lockdown, forcing children to stay at home. The authors aimed to analyze the change in the pattern of trauma-related visits during the lockdown. Methods: We carried out a retrospective descriptive study on injured children aged 17 years or younger who visited the emergency department of a tertiary pediatric hospital in Bogotá, Colombia from March 15 through May 15, 2019 (control period) and the same period in 2020 (lockdown period). Between the 2 periods, baseline characteristics and injury profiles were compared. Results: Among the study population (n = 1,485), 1,122 and 363 children visited the emergency department during the control and lockdown periods, respectively. In the midst of 73.9% decrease in numbers of overall visits between the 2 periods, a 67.6% decrease was noted in number of trauma-related visits. Regarding the proportions, trauma-related visits increased from 7.9% to 9.8%. During the lockdown, increases occurred in the proportions of the following variables: children younger than 5 years (25.5% to 50.7%; P < 0.001), mechanisms other than blunt, minor fall or traffic accident (e.g., bite, 3.9% to 6.6%; P = 0.032), child abuse (1.2% to 4.1%; P = 0.003), hospitalization (4.6% to 35.8%; P < 0.001), open wound (21.1% to 36.9%; P < 0.001), the use of computed tomography (6.3% to 9.9%; P < 0.001), and abnormal imaging findings (28.8% to 31.7%; P = 0.003). Conclusion: During the lockdown, children with trauma may show an increase in overall severity, and also a higher risk of abusive trauma. This finding indicates a sensible need of educating families in prevention of domestic injury.

Removal of textile dyes in wastewater using polyelectrolytes containing tetrazole groups

Martín Caldera-Vil,Alejandra-Alicia Peláez-Cid,Miriam-Amelia Martins-Alho,Ana-María Herrera-González 한국화학공학회 2018 Korean Journal of Chemical Engineering Vol.35 No.12

Textile dyes are some of the pollutants which have received the most attention because of the large volume of wastewater generated by the textile industry. Removal by means of adsorption is one of the most versatile alternatives to treat these effluents. Even though different adsorbents such as activated carbons and mineral materials have been proposed, polymeric adsorbents are a viable alternative. This work reports for the first time the use of polyelectrolyte PTZ and macroelectrolyte MTZ containing tetrazole groups as adsorbents useful in the textile dyes removal present in aqueous solutions and wastewater. Because of the anionic character of the tetrazole group, MTZ exhibits selective adsorption capabilities for cationic dyes of up to 156.25mg·g1. The kinetic study of the process of adsorption shows that PTZ and MTZ fit a pseudo second-order model. MTZ also shows utility as a flocculant agent in the treatment of wastewater containing dyes Indigo Blue and Reactive Black. The results showed that PTZ and MTZ may be used in the treatment of wastewater in a process of coagulation-flocculation followed by the treatment by adsorption. This twostage treatment removed up to 95% of the dye present in the wastewater. As well as removing the dyes, the values for COD, suspended solids, pH, and color of the wastewater decreased, thus significantly improving its quality.

Oxidative response of neutrophils to platelet-activating factor is altered during acute ruminal acidosis induced by oligofructose in heifers

Claudia Concha,María Daniella Carretta,Pablo Alarcón,Ivan Conejeros,Diego Gallardo,Alejandra Isabel Hidalgo,Nestor Tadich,Dante Daniel Cáceres,María Angélica Hidalgo,Rafael Agustín Burgos 대한수의학회 2014 Journal of Veterinary Science Vol.15 No.2

Reactive oxygen species (ROS) production is one of the mainmechanisms used to kill microbes during innate immuneresponse. D-lactic acid, which is augmented during acuteruminal acidosis, reduces platelet activating factor(PAF)-induced ROS production and L-selectin shedding inbovine neutrophils in vitro. This study was conducted toinvestigate whether acute ruminal acidosis induced by acuteoligofructose overload in heifers interferes with ROSproduction and L-selectin shedding in blood neutrophils. Blood neutrophils and plasma were obtained by jugularvenipuncture, while ruminal samples were collected usingrumenocentesis. Lactic acid from plasma and ruminal sampleswas measured by HPLC. PAF-induced ROS production andL-selectin shedding were measured in vitro in bovineneutrophils by a luminol chemiluminescence assay and flowcytometry, respectively. A significant increase in ruminal andplasma lactic acid was recorded in these animals. Specifically,a decrease in PAF-induced ROS production was observed 8 hafter oligofructose overload, and this was sustained until 48 hpost oligofructose overload. A reduction in PAF-inducedL-selectin shedding was observed at 16 h and 32 h postoligofructose overload. Overall, the results indicated thatneutrophil PAF responses were altered in heifers with ruminalacidosis, suggesting a potential dysfunction of the innateimmune response.

Restoration and conservation of anatomic pieces

Camila Cárdenas Guerrero Guzmán,Karen Alejandra Pérez Díaz,María Paula Ruíz Díaz,Valentina Díaz Sánchez,Andrés Camilo Ariza Aguirre,Laura Catalina Cantor Alfonso,Camila Andrea Suárez Ortiz,Davide Fali 대한해부학회 2019 Anatomy & Cell Biology Vol.52 No.3

In this study, a restoration process was developed with potassium hydroxide (KOH), in order to improve each of the structures for their posterior fixation, through the use of new methods such as the Chilean conservative fixative solution (SFCCh), with exceptional results. Restore anatomical pieces corresponding to corpse and organs, being these last set with the SFCCh. In this work dealt with processes of restoration with potassium hydroxide, sodium chloride, and sodium hypochlorite, the process began with the cleanliness and suture of the structures for subsequent fixing in Chilean conservative fixative solution, making use of a corpse and different anatomical parts. Work based on items found in the database, Elsevier, Science Direct, ProQuest, and MEDLINE. At the end of the process of restoration and conservation of the anatomical pieces, was observed an improvement in muscle pigment with decrease of rigidity in the specimen, additionally a recovery of appearance in the vascular-nervous elements was achieved. The organs were much more malleable and the structures facilitate the identification of specific details, its subsequent immersion in SFCCh allows the longer preservation of the obtained results. The restoration with potassium hydroxide allows the improvement in the appearance of the different anatomical structures and simultaneously to facilitate its study. The SFCCh is an alternative that replaces partially the use of formaldehyde. In addition, it presents toxicity reduction.

Homozygous LPL p.Gly188Glu Mutation in a Mexican Girl With Lipoprotein Lipase Deficiency

Ana Gabriela Colima Fausto,Juan Ramón González García,Teresita De Jesús Hernández Flores,Norma Alejandra Vázquez Cárdenas,Nery Eduardo Solís Perales,María Teresa Magaña Torres 대한진단검사의학회 2017 Annals of Laboratory Medicine Vol.37 No.4

Dear Editor, Lipoprotein lipase (LPL) deficiency is a rare disease characterized by severe hypertriglyceridemia due to mutations in the LPL gene. It shows an autosomal recessive inheritance pattern and has a general prevalence of 1:1,000,000 [1]. Patients typically present abdominal pain, hemorrhage, failure to thrive, jaundice, eruptive xanthomas, lipemia retinalis, neurological complications, hepatosplenomegaly, and life-threatening pancreatitis [2]. The LPL gene is located on chromosomal band 8p22, contains 10 exons, and codes for a 475-amino acid protein that is active only in its dimeric form. The LPL protein has two functions in lipid metabolism: it hydrolyzes triglycerides and functions as a ligand [3]. The aim of this work is to describe the biochemical, clinical, and molecular features of a Mexican girl with LPL deficiency.

Application of Molecular Methods for the Identification of Acetic Acid Bacteria Isolated from Blueberries and Citrus Fruits

Liliana Mabel Gerard,Cristina Verónica Davies,Carina Alejandra Soldá,María Belén Corrado,María Verónica Fernández 한국미생물·생명공학회 2020 한국미생물·생명공학회지 Vol.48 No.2

Sixteen acetic acid bacteria (AAB) were isolated from blueberries and citric fruits of the Salto Grande region (Concordia, Entre Ríos, Argentina) using enrichment techniques and plate isolation. Enrichment broths containing ethanol and acetic acid enabled maximum AAB recovery, since these components promote their growth. Biochemical tests allowed classification of the bacteria at genus level. PCR-RFLP of the 16S rRNA and PCR-RFLP of the 16S-23S rRNA intergenic spacer allowed further classification at the species level; this required treatment of the amplified products of 16S and 16S-23S ITS ribosomal genes with the following restriction enzymes: AluI, RsaI, HaeIII, MspI, TaqI, CfoI, and Tru9I. C7, C8, A80, A160, and A180 isolates were identified as Gluconobacter frateurii; C1, C2, C3, C4, C5, C6, A70, and A210 isolates as Acetobacter pasteurianus; A50 and A140 isolates as Acetobacter tropicalis; and C9 isolate as Acetobacter syzygii. The bacteria identified by 16S rRNA PCR-RFLP were validated by 16S-23S PCR-RFLP; however, the C1 isolate showed different restriction patterns during identification and validation. Partial sequencing of the 16S gene resolved the discrepancy.

Restoration and conservation of anatomic pieces

Camila Cá,rdenas Guerrero Guzmá,n,Karen Alejandra Pé,rez Dí,az,Marí,a Paula Ruí,z Dí,az,Valentina Dí,az Sá,nchez,André,s Camilo Ariza Aguirre,Laura Catalina Can 대한해부학회 2019 Anatomy & Cell Biology Vol.52 No.3

In this study, a restoration process was developed with potassium hydroxide (KOH), in order to improve each of the structures for their posterior fixation, through the use of new methods such as the Chilean conservative fixative solution (SFCCh), with exceptional results. Restore anatomical pieces corresponding to corpse and organs, being these last set with the SFCCh. In this work dealt with processes of restoration with potassium hydroxide, sodium chloride, and sodium hypochlorite, the process began with the cleanliness and suture of the structures for subsequent fixing in Chilean conservative fixative solution, making use of a corpse and different anatomical parts. Work based on items found in the database, Elsevier, Science Direct, ProQuest, and MEDLINE. At the end of the process of restoration and conservation of the anatomical pieces, was observed an improvement in muscle pigment with decrease of rigidity in the specimen, additionally a recovery of appearance in the vascular-nervous elements was achieved. The organs were much more malleable and the structures facilitate the identification of specific details, its subsequent immersion in SFCCh allows the longer preservation of the obtained results. The restoration with potassium hydroxide allows the improvement in the appearance of the different anatomical structures and simultaneously to facilitate its study. The SFCCh is an alternative that replaces partially the use of formaldehyde. In addition, it presents toxicity reduction.