A retrospective overview of Intelligent Transportation Systems Deployment in Korea

( Akhtar Ali Shah ),이종달 ( Jong Dal Lee ) 영남대학교 영남지역발전연구소 2006 영남지역발전연구 Vol.35 No.-

Effects of peripartal glucose precursor supplementation on lactation performance and metabolic health of primiparous and multiparous dairy cows

Akhtar Muhammad Uzair,Hifzulrahman,Pasha Talat Naseer,Avais Muhammad,Khan Nauman,Chishti Ghazanfar Ali,Ali Mubashar,Imran Muhammad,Tahir Muhammad Naeem,Naveed-ul-Haque Muhammad 아세아·태평양축산학회 2023 Animal Bioscience Vol.36 No.6

Objective: Hyperketonemia remains a major metabolic issue of serious milk production and a major health concern in early lactation cows. Oral supplementation of glucose precursors (GP) can be used to prevent hyperketonemia in dairy cows. The objective of this study was to compare the beneficial effects of orally supplementing a mixture of GP on metabolic health indicators and milk production status of primiparous (PP) and multiparous (MP) dairy cows. Methods: Twenty-eight Holstein cows were blocked by expected date of parturition, previous lactation yield, and parity. The cows were randomly allocated to one of the four treatment groups (n = 7 cows/group) based on their parity and GP supplementation: i) PP cows fed basal diet only (PP-CON), ii) PP cows with oral supplementation of GP (PP-GP), iii) MP cows fed basal diet only (MP-CON), and iv) MP cows with oral supplementation of GP (MP-GP). Glucose precursor (glycoline liquid) was orally drenched (300 mL/d) in GP cows from 7 days prepartum through 7 days postpartum. Other than GP supplementation, all cows were fed similar pre- and postpartum basal diets. Results: In both pre- and postpartum periods, serum glucose concentration was increased, whereas β-hydroxybutyrate and free fatty acids were decreased in GP cows compared with the CON cows. Milk yield and milk components were statistically not different between GP and CON cows over the first 9 week of lactation. The yield of actual milk, energycorrected milk, 63-days cumulative milk, colostrum yield, and calf birth weight remained higher in MP cows compared with PP cows. Conclusion: Oral drenching of GP around calving can be recommended to successfully improve the metabolic health and reduce the negative effects of hyperketonemia not only in MP but also in PP dairy cows.

Arabidopsis HOS15 is a multifunctional protein that negatively regulate ABA-signaling and drought stress

Akhtar Ali,윤대진 한국식물생명공학회 2020 Plant biotechnology reports Vol.14 No.2

Phytohormone abscisic acid (ABA) plays a major role in plant adaptation to environmental stresses. ABA accumulates in response to water defi cit situation and increase in ABA level promotes stomatal closure and thereby reduces leaf transpiration rate. HOS15, a DDB1-CUL4 Associated protein, was previously shown to regulate several physiological functions including freezing stress, fl owering, leaf morphology, ABA-signaling and drought stress. In this review, we focused on the involvement of HOS15 in ABA-signaling. HOS15 interact with and negatively regulate OST1 which lead to fi ne tune ABA signaling deactivation. In fact, HOS15 is one of the major missing components in ABA-signaling core that inter-connect activation and de-activation of ABA-signaling.

Speech Feature Selection of Normal and Autistic children using Filter and Wrapper Approach

Akhtar, Muhammed Ali,Ali, Syed Abbas,Siddiqui, Maria Andleeb International Journal of Computer ScienceNetwork S 2021 International journal of computer science and netw Vol.21 No.5

Two feature selection approaches are analyzed in this study. First Approach used in this paper is Filter Approach which comprises of correlation technique. It provides two reduced feature sets using positive and negative correlation. Secondly Approach used in this paper is the wrapper approach which comprises of Sequential Forward Selection technique. The reduced feature set obtained by positive correlation results comprises of Rate of Acceleration, Intensity and Formant. The reduced feature set obtained by positive correlation results comprises of Rasta PLP, Log energy, Log power and Zero Crossing Rate. Pitch, Rate of Acceleration, Log Power, MFCC, LPCC is the reduced feature set yield as a result of Sequential Forwarding Selection.

Salt stress tolerance; what do we learn from halophytes?

Akhtar Ali,윤대진 한국식물학회 2017 Journal of Plant Biology Vol.60 No.5

Abiotic environmental stresses can give rise tomorphological, biochemical and molecular changes thatnegatively affect plant growth and productivity. Amongthese stresses, soil salinity is the major threat. To deal andcontrol effects of high salinity on plants, it is important tounderstand their responses to salt stress that disturbs thehomeostatic equilibrium at cellular and molecular levels. Inthis regard halophytes (salt tolerant plants) can providesuperior models for the study of salt stress defense parameterscompared to salt sensitive species (glycophytes). Halophytesuse highly developed, complex systems to tolerate salinityby maintaining a low cytosolic Na+/K+ ratio, sequestrationof Na+ into vacuoles that then provides the osmotic potentialsustaining water influx. Under low intensity stress conditionsthat moderately and/or transiently affect ion imbalance, theset of responses all plants initiate will be mostly to engagemeasures that assure ion balance. High salinity, especiallyover a prolonged time period, will challenge plant survival,which then requires different strategies that employ a variety ofmechanisms. Plasticity and connectivity of these diversemechanisms is engrained in species- and family-specificevolutionary history and their genetic complexity. Highlightingdifferences in the genetic and biochemical makeup betweenglycophytes and halophytes allows for comparisons betweentheir approaches towards high salinity. This review provides abrief overview about different strategies and mechanismused by plants to avoid or confine adverse effects of highsalinity.

Role and Functional Differences of HKT1-Type Transporters in Plants under Salt Stress

Ali, Akhtar,Maggio, Albino,Bressan, Ray A.,Yun, Dae-Jin MDPI 2019 INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES Vol.20 No.5

<P>Abiotic stresses generally cause a series of morphological, biochemical and molecular changes that unfavorably affect plant growth and productivity. Among these stresses, soil salinity is a major threat that can seriously impair crop yield. To cope with the effects of high salinity on plants, it is important to understand the mechanisms that plants use to deal with it, including those activated in response to disturbed Na<SUP>+</SUP> and K<SUP>+</SUP> homeostasis at cellular and molecular levels. HKT1-type transporters are key determinants of Na<SUP>+</SUP> and K<SUP>+</SUP> homeostasis under salt stress and they contribute to reduce Na<SUP>+</SUP>-specific toxicity in plants. In this review, we provide a brief overview of the function of HKT1-type transporters and their importance in different plant species under salt stress. Comparison between HKT1 homologs in different plant species will shed light on different approaches plants may use to cope with salinity.</P>

Quercetin induces cell death in cervical cancer by reducing O-GlcNAcylation of adenosine monophosphate-activated protein kinase

Akhtar Ali,김민준,김민영,이한주,노구석,김현준,조경재,최완성 대한해부학회 2018 Anatomy & Cell Biology Vol.51 No.4

Hyper-O-GlcNAcylation is a general feature of cancer which contributes to various cancer phenotypes, including cell proliferation and cell growth. Quercetin, a naturally occurring dietary flavonoid, has been reported to reduce the proliferation and growth of cancer. Several reports of the anticancer effect of quercetin have been published, but there is no study regarding its effect on O-GlcNAcylation. The aim of this study was to investigate the anticancer effect of quercetin on HeLa cells and compare this with its effect on HaCaT cells. Cell viability and cell death were determined by MTT and terminal deoxynucleotidyl transferase–mediated deoxyuridine triphosphate nick-end labelling assays. O-GlcNAcylation of AMP-activated protein kinase (AMPK) was examined by succinylated wheat germ agglutinin pulldown and immunoprecipitation. Immunofluorescence staining was used to detect the immunoreactivitiy of O-linked N-acetylglucosamine transferase (OGT) and sterol regulatory element binding protein 1 (SREBP-1). Quercetin decreased cell proliferation and induced cell death, but its effect on HaCaT cells was lower than that on HeLa cells. O-GlcNAcylation level was higher in HeLa cells than in HaCaT cells. Quercetin decreased the expression of global O-GlcNAcylation and increased AMPK activation by reducing the O-GlcNAcylation of AMPK. AMPK activation due to reduced O-GlcNAcylation of AMPK was confirmed by treatment with 6-diazo-5-oxo-L-norleucine. Our results also demonstrated that quercetin regulated SREBP-1 and its transcriptional targets. Furthermore, immunofluorescence staining showed that quercetin treatment decreased the immunoreactivities of OGT and SREBP-1 in HeLa cells. Our findings demonstrate that quercetin exhibited its anticancer effect by decreasing the O-GlcNAcylation of AMPK. Further studies are needed to explore how quercetin regulates O-GlcNAcylation in cancer.

O-GlcNAcylation of NF- B Promotes Lung Metastasis of Cervical Cancer Cells via Upregulation of CXCR4 Expression

Akhtar Ali,김성환,김민준,최미영,강상수,조경제,김윤숙,최준영,최완성 한국분자세포생물학회 2017 Molecules and cells Vol.40 No.7

C-X-C chemokine receptor 4 (CXCR4) stimulates cancer metastasis. NF- B regulates CXCR4 expression in cancer cells, and O-GlcNAc modification of NF- B promotes its transcriptional activity. Here, we determined whether CXCR4 expression is affected by O-GlcNAcylation of NF- B in lung metastasis of cervical cancer. We found elevated levels of O-linked-N-actylglucosamine transferase (OGT) and O-GlcNAcylation in cervical cancer cells compared to those in non-malignant epithelial cells and detected increased expression of NF- B p65 (p65) and CXCR4 in cervical cancer cells. Knockdown of OGT inhibited the O-GlcNAcylation of p65 and decreased CXCR4 expression levels in HeLa cells. Thiamet G treatment increased O-GlcNAcylated p65, which subsequently enhanced CXCR4 expression levels. Inhibition of O-GlcNAcylation by 6-Diazo-5-oxo-L-norleucine (DON) treatment decreased p65 activation, eventually inhibiting CXCR4 expression in HeLa cells. Lung tissues from mice engrafted with OGT-knockdown HeLa cells (shOGT) exhibited lower expression of Ki 67 and HPV E6 and E7 oncogenes com-pared to lung tissues from mice engrafted with control HeLa cells (shCTL). In addition, lung tissues from mice engrafted with shOGT cells exhibited lower p65 and CXCR4 immunoreactivity compared to tissues from mice engrafted with shCTL cells. Taken together, our data suggest that p65 O-GlcNAcylation promotes lung me-tastasis of cervical cancer cells by activating CXCR4 expression.

A Single Amino-Acid Substitution in the Sodium Transporter HKT1 Associated with Plant Salt Tolerance

Ali, Akhtar,Raddatz, Natalia,Aman, Rashid,Kim, Songmi,Park, Hyeong Cheol,Jan, Masood,Baek, Dongwon,Khan, Irfan Ullah,Oh, Dong-Ha,Lee, Sang Yeol,Bressan, Ray A.,Lee, Keun Woo,Maggio, Albino,Pardo, Jose American Society of Plant Biologists 2016 Plant Physiology Vol.171 No.3

<P>A crucial prerequisite for plant growth and survival is the maintenance of potassium uptake, especially when high sodium surrounds the root zone. The Arabidopsis HIGH-AFFINITY K+ TRANSPORTER1 (HKT1), and its homologs in other salt-sensitive dicots, contributes to salinity tolerance by removing Na+ from the transpiration stream. However, TsHKT1; 2, one of three HKT1 copies in Thellungiella salsuginea, a halophytic Arabidopsis relative, acts as a K+ transporter in the presence of Na+ in yeast (Saccharomyces cerevisiae). Amino-acid sequence comparisons indicated differences between TsHKT1; 2 and most other published HKT1 sequences with respect to an Asp residue (D207) in the second pore-loop domain. Two additional T. salsuginea and most other HKT1 sequences contain Asn (N) in this position. Wild-type TsHKT1; 2 and altered AtHKT1 (AtHKT1(N-D)) complemented K+-uptake deficiency of yeast cells. Mutant hkt1-1 plants complemented with both AtHKT1(N-D) and TsHKT1; 2 showed higher tolerance to salt stress than lines complemented by the wild-type AtHKT1. Electrophysiological analysis in Xenopus laevis oocytes confirmed the functional properties of these transporters and the differential selectivity for Na+ and K+ based on the N/D variance in the pore region. This change also dictated inward-rectification for Na+ transport. Thus, the introduction of Asp, replacing Asn, in HKT1-type transporters established altered cation selectivity and uptake dynamics. We describe one way, based on a single change in a crucial protein that enabled some crucifer species to acquire improved salt tolerance, which over evolutionary time may have resulted in further changes that ultimately facilitated colonization of saline habitats.</P>

Differential selection of sodium and potassium ions by TsHKT1;2

Ali, Akhtar,Yun, Dae-Jin Informa UK (Taylor Francis) 2016 Plant signaling & behavior Vol.11 No.8