부산지역 유흥업소 종사여성으로부터 분리된 HPV16형의 발암유전자(E6/E7) 돌연변이 유형 분석

민상기(Sang-Kee Min),김성순(Sung Soon Kim),최병선(Byeong-Sun Choi),장대호(Dai-Ho Jang),이미옥(Mee-Ok Lee),최성화(Seung-Hwa Choi),김남호(Nam-Ho Kim),박연경(Yon-Koung Park),정영아(Yeong-A Jeong),김성준(Seong-Joon Kim),빈재훈(Jae-Hun Bi 한국생명과학회 2009 생명과학회지 Vol.19 No.6

HPV-16형의 염기배열 변이는 지역적, 인종적으로 특징적인 차이가 있으며 특히 HPV-16형 E6/E7 유전자의 특정 염기서열변이는 자궁경부암 및 자궁상피내 신생종양물의 발생을 일으키는 고위험 요인으로 알려져 있다. 본 연구는 2007년 부산지역 유흥업소 종사여성으로 분리된 HPV-16형 19건을 대상으로 E6/E7 유전자 영역(nt 34-880)을 표적으로 지역적 염기서열 변이를 조사하였다. nucleotide 수준에서 HPV16형 E6 유전자는 T178G (n=11), T178A (n=1), T350G (n=4), A442C (n=2), A104T, A111G, C116T, G145T, T183G, C335T, G522C 등 11종의 변이주가 발견되었고, E7 유전자는 A647G (n=12), A645C, A777C, G663A, T732C, T760C, A775T, T789C, T795G 등 9종의 변이주가 발견되었다. 아미노산 수준에서는 HPV-16형 E6 단백질의 경우 D25E (n=12), L83V (n=4), E113D (n=2), M1L, Q3R, P5S, Q14H, D25N, I27R, H78Y, C140S 등 11종의 변이주를, HPV16형 E7 단백질의 경우 N29S (n=12), L28F, T72S 등 3종의 변이주를 관찰할 수 있었다. 본 연구 결과, 부산지역의 HPV-16형 E6/E7 우점 돌연변이주는 E6 D25E (75%), E7 N29S (78%)로 각각 나타났다. 앞으로 자궁경부암 환자 및 일반여성을 포함한 더 많은 모집단을 대상으로 HPV-16형 E6/E7의 intratypic variants를 비교 조사하여 실제 HPV-16형 E6/E7 어떤 변이주가 자궁경부암 유발 위험성과의 관련성은 더 많이 연구되어져야 할 것으로 사료된다. Recent studies have reported that the distribution of HPV-16 sequence variation differs geographically, and more specifically that HPV-16 E6/E7 intratypic variants might carry a high risk for development of ICC (invasive cervical cancer) and CIN (cervical intraepithelial neoplasia) in a given population. To investigate the genetic diversities of HPV-16 E6/E7 oncogene by region, we collected nineteen HPV-16 isolates from sexually high-risk women in Busan, and analyzed the HPV-16 E6/E7 coding regions (nt 34 to 880) with HPV-16 E6/E7 specific PCR amplification. At the nucleotide level, eleven variants of the E6 genes and nine variants of the E7 genes were identified as follows: E6 T178G (n=11), E6 T178A (n=1), E6 T350G (n=3), E6 A442C (n=2), E6 A104T, E6 A111G, E6 C116T, E6 G145T, E6 T183G, E6 C335T, E6 G522C and E7 A647G (n=12), E7 A645C, E7 A777C, E7 G663A, E7 T732C, E7 T760C, E7 A775T, E7 T789C and E7 T795G, respectively. At the amino acid level, the isolated HPV-16 E6 and E7 genes showed eleven E6 variants: E6 D25E (n=12), E6 L83V (n=4), E6 E113D (n=2), E6 M1L, E6 Q3R, E6 P5S, E6 Q14H, E6 D25N, E6 I27R, E6 H78Y, E6 C140S and three E7 variants: N29S (n=12), L28F, T72S. HPV16 E6 L83V, the dominant variant in the Caucasian population, showed relatively low frequencies in our study population. We elucidated that the dominant HPV-16 E6/E7 variants were HPV-16 E6 D25E (63.2%) and HPV-16 E7 N29S (63.2%), which were phylogenetically included in Asian lineage. Further study is needed to evaluate the risk of cervical cancer related HPV-16 E6/E7 intratypic variants in the Korean population.

사이토카이닌과 에틸렌, 프로피코나졸 복합처리에 따른 고년근 인삼 생육 및 진세노사이드 함량 변화

권나영(Na Yeong Kwon),이정우(Jung Woo Lee),정성민(Sung Min Jung),조익현(Ick Hyun Jo),장인복(In Bok Jang),방경환(Kyong Hwan Bang),김영창(Young Chang Kim),김동휘(Dong Hwi Kim),황현아(Hyeon A Hwang),임유경(Yoo Kyung Lim),조현우(Hyun Woo 한국약용작물학회 2023 한국약용작물학회지 Vol.31 No.1

Background: Korean ginseng (Panax ginseng C. A. Meyer) is recognized for its medicinal properties. Because ginseng is cultivated for 4 – 6 years in one place, the growing environment can affect ginseng growth. Specifically, root growth can be altered by plant hormones, which in turn, affects crop yields. Among plant hormones, cytokinin is known to increase secondary growth by promoting cambium cell division. In addition, ethylene and propiconazole can regulate root development. In this study, the effects and interactions of cytokinin, ethylene, and propiconazole on four-year-old ginseng plants were analyzed. Methods and Results: Ginseng plants were treated with each hormone every two weeks. Cytokinin (6-benzylaminopurine, 500 μM) was applied to the soil, and 100 nM of propiconazole or 100 μM of ethylene was applied in combination with cytokinin. Root width and weight, and ginsenoside content of each plant were the highest when cytokinin and propiconazole were applied together. The highest number of buds was observed under cytokinin treatment. However, no significant differences were observed in shoot growth, except for stem width. Conclusions: The greatest effect on root development was observed when cytokinin and propiconazole were applied together, and ginsenoside content increased following hormone treatment.

Anti-inflammatory properties of neutral lipids, glycolipids, and phospholipids isolated from Ammodytes personatus eggs in LPS-stimulated RAW264.7 cells

Gyoung Su Choi,A-Yeong Jang,Weerawan Rod-In,Woo Jung Park 한국식품영양과학회 2021 한국식품영양과학회 학술대회발표집 Vol.2021 No.10

Total lipids were extracted from A. personatus (Pacific sand lance) eggs, and then they were separated into neutral lipids, glycolipids and phospholipids. Anti-inflammatory activities of three lipids including neutral lipids, glycolipids and phospholipids isolated from A. personatus eggs were determined by the production of nitric oxide (NO), the gene expression, and the protein expression in LPS-stimulated RAW264.7 murine macrophages. The cells were pre-treated with the lipids at various concentration (0.5, 1.0, 1.5, and 2.0%), and stimulated with LPS. The results demonstrated that three lipids significantly reduced the NO production and the mRNA expression of immune-associated genes, such as iNOS, COX-2, IL-1β, IL-6, and TNF-α, in dose-dependent manner. All lipids also down-regulated the protein expression of phosphorylated NF-κB-p65 and MAPKs (p38, JNK and ERK1/2) signaling pathway. These results suggest that neutral lipids, glycolipids and phospholipids isolated from A. personatus egg exerts the anti-inflammatory activities via the inhibition of NF-κB and MAPKs activation.

Korean Ginseng Berry Polysaccharide Enhances Immunomodulation Activities of Peritoneal Macrophages in Mice with Cyclophosphamide-Induced Immunosuppression

Choi JeongUn,Nam Ju Hyun,Rod-in Weerawan,Monmai Chaiwat,Jang A-yeong,You SangGuan,Park Woo Jung 한국미생물·생명공학회 2023 Journal of microbiology and biotechnology Vol.33 No.6

Korean ginseng (Panax ginseng C. A. Meyer), a member of the Araliaceae family, is known as a traditional medicinal plant to have a wide range of health properties. Polysaccharides constitute a major component of Korean ginseng, and its berries exhibit immune-modulating properties. The purpose of this study was to investigate the immune effects of crude polysaccharide (GBPC) extracted from Korean ginseng berry on peritoneal macrophages in mice with cyclophosphamide (CY)- induced immunosuppression. BALB/c mice were divided into eight groups: normal control, normal control + CY, levamisole + CY, ginseng + CY, and four concentrations of 50, 100, 250, and 500 mg/kg BW/day of GBPC + CY. Mice were orally administered with samples for 10 days. Immunosuppression was established by treating mice with CY (80 mg/kg BW/day) through intraperitoneal injection on days 4 to 6. The immune function of peritoneal macrophages was then evaluated. Oral administration of 500 mg/kg BW/day GBPC resulted in proliferation, NO production, and phagocytosis at 100%, 88%, and 91%, respectively, close to the levels of the normal group (100%) of peritoneal macrophages. In CY-treated mice, GBPC of 50−500 mg/kg BW/day also dosedependently stimulated the proliferation, NO production, and phagocytosis at 56−100%, 47−88%, and 53−91%, respectively, with expression levels of immune-associated genes, such as iNOS, COX−2, IL−1β, IL−6, and TNF–α, of about 0.32 to 2.87-fold, compared to those in the CY group. GBPC could be a potential immunomodulatory material to control peritoneal macrophages under an immunosuppressive condition.

Immune-Enhancing Effects of Crude Polysaccharides from Korean Ginseng Berries on Spleens of Mice with Cyclophosphamide-Induced Immunosuppression

( Ju Hyun Nam ),( Jeongun Choi ),( Chaiwat Monmai ),( Weerawan Rod-in ),( A-yeong Jang ),( Sangguan You ),( Woo Jung Park ) 한국미생물 · 생명공학회 2022 Journal of microbiology and biotechnology Vol.32 No.2

Panax ginseng C. A. Meyer is well known as traditional herbal medicine, and ginseng berries are known to exhibit potential immune-enhancing functions. However, little is known about the in vivo immunomodulatory activity of Korean ginseng berries. In this study, crude Korean ginseng berries polysaccharides (GBP) were isolated and their immunomodulatory activities were investigated using cyclophosphamide (CY)-induced immunosuppressive BALB/c mice. In CY-treated mice, oral administration of GBP (50-500 mg/kg BW) remarkably increased their spleen sizes and spleen indices and activated NK cell activities. GBP also resulted in the proliferation of splenic lymphocytes (coordinating with ConA: plant mitogen which is known to stimulate T-cell or LPS: endotoxin which binds receptor complex in B cells to promote the secretion of pro-inflammatory cytokines) in a dosedependent manner. In addition, GBP significantly stimulated mRNA expression levels of immuneassociated genes including interleukin-1β (IL-1β), IL-2, IL-4, IL-6, tumor necrosis factor-α (TNF-α), interferon-γ (IFN-γ), toll-like receptor 4 (TLR-4), and cyclooxygenase-2 (COX-2) in CY-treated mice. These results indicate that GBP is involved in immune effects against CY-induced immunosuppression. Thus, GBP could be developed as an immunomodulation agent for medicinal or functional food application.

Monte Carlo Study on the Wetting Behavior of a Surface Texturized with Domed Pillars

Kim, Hyojeong,Lee, Su In,Matin, Mohammad A.,Zhang, Zhengqing,Jang, Jihye,Ha, Man Yeong,Jang, Joonkyung American Chemical Society 2014 The Journal of Physical Chemistry Part C Vol.118 No.45

<P>A lattice gas Monte Carlo simulation was performed to examine the wetting properties of a surface texturized with nanometer-sized, dome-shaped pillars. The vapor and liquid phases of the gap between the pillars were related to the Wenzel and Cassie–Baxter states of a macroscopic water droplet resting on top of the pillars. We studied the effects of the pillar size by systematically varying its height from 6 to 53 nm for a fixed ratio of the height to its width. With increasing interpillar spacing or pressure, the liquid on top of the domed pillars penetrated smoothly down into the gap between the pillars. This wetting transition contrasts with that observed for the gap between rectangular or cylindrical pillars, where a liquid abruptly fills in the interpillar gap at a critical interpillar spacing or pressure. The gap between the domed pillars was more susceptible to the intrusion of the bulk liquid on top of the pillars, due to the open geometry of the gap between the domed pillars. Also, the liquid penetrating into the gap between the domed pillars was locally more fluctuating in density and compressible than that penetrating into the gap between square or cylindrical pillars. This enhanced density fluctuation however was local and did not propagate into the bulk liquid sitting on top of the pillars. Simple analytic expressions of the critical spacing and pressure at which the wetting transition occurs for the domed pillars were derived using continuum theory. These continuum results agreed reasonably well with the present molecular simulations, even for pillars as small as a few nanometers in width.</P><P><B>Graphic Abstract</B> <IMG SRC='http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/jpccck/2014/jpccck.2014.118.issue-45/jp5076077/production/images/medium/jp-2014-076077_0013.gif'></P><P><A href='http://pubs.acs.org/doi/suppl/10.1021/jp5076077'>ACS Electronic Supporting Info</A></P>

Development of an S-band cavity-type beam position monitor for a high power THz free-electron laser

Noh, Seon Yeong,Kim, Eun-San,Hwang, Ji-Gwang,Heo, A.,won Jang, Si,Vinokurov, Nikolay A.,Jeong, Young UK,Hee Park, Seong,Jang, Kyu-Ha American Institute of Physics 2015 Review of scientific instruments Vol.86 No.1

<P>A cavity-type beam position monitor (BPM) has been developed for a compact terahertz (THz) free-electron laser (FEL) system and ultra-short pulsed electron Linac system at the Korea Atomic Energy Research Institute (KAERI). Compared with other types of BPMs, the cavity-type BPM has higher sensitivity and faster response time even at low charge levels. When electron beam passes through the cavity-type BPM, it excites the dipole mode of the cavity of which amplitude depends linearly on the beam offset from the center of the cavity. Signals from the BPM were measured as a function of the beam offset by using an oscilloscope. The microtron accelerator for the KAERI THz FEL produces the electron beam with an energy of 6.5 MeV and pulse length of 5 μs with a micropulse of 10-20 ps at the frequency of 2.801 GHz. The macropulse beam current is 40 mA. Because the microtron provides multi-bunch system, output signal would be the superposition of each single bunch. So high output signal can be obtained from superposition of each single bunch. The designed position resolution of the cavity-type BPM in multi-bunch is submicron. Our cavity-type BPM is made of aluminum and vacuum can be maintained by indium sealing without brazing process, resulting in easy modification and cost saving. The resonance frequency of the cavity-type BPM is 2.803 GHz and the cavity-type BPM dimensions are 200 220 mm (length height) with a pipe diameter of 38 mm. The measured position sensitivity was 6.19 (mV/mm)/mA and the measured isolation between the X and Y axis was -39 dB. By measuring the thermal noise of system, position resolution of the cavity-type BPM was estimated to be less than 1 μm. In this article, we present the test results of the S-band cavity-type BPM and prove the feasibility of the beam position measurement with high resolution using this device.</P>

A systematic study on molecular planarity and D–A conformation in thiazolothiazole- and thienylenevinylene-based copolymers for organic field-effect transistors

Lim, Dae-Hee,Jang, Soo-Young,Kang, Minji,Lee, Sehyun,Kim, Yeong-A,Heo, Youn-Jung,Lee, Min-Hye,Kim, Dong-Yu The Royal Society of Chemistry 2017 Journal of Materials Chemistry C Vol.5 No.39

<▼1><P>The new D–A type conjugated polymers were synthesized to confirm that structural characteristics, such as the presence of coplanar units and a D–A conformation.</P></▼1><▼2><P>A new donor–acceptor type conjugated polymer (P1) composed of thiazolothiazole (TzTz) and thienylenevinylene (TV) units was synthesized to confirm that structural characteristics, such as the presence of coplanar units and a D–A conformation in the polymer main chain, can have an effect on charge carrier transport in high performance organic field-effect transistors. P1 was compared with two polymers that had bithiophene (BiTh) units instead of TV units (P2) or thienothiophene (TT) units in the place of TzTz units (P3) to investigate the relationship between the molecular structure and polymer properties. P1 had a higher order of orientation than P2 due to the rigid and planar structure caused by the presence of the TV unit in P1 compared to the distorted structure of P2. The effect of the D–A type configuration in P1 was also confirmed by demonstrating its stronger intermolecular interactions and more effective hole and electron transport compared with that of the D–D type polymer P3. Thus, in contrast to P2 (with a hole mobility of 0.076 cm<SUP>2</SUP> V<SUP>−1</SUP> s<SUP>−1</SUP> and an electron mobility of 0.0025 cm<SUP>2</SUP> V<SUP>−1</SUP> s<SUP>−1</SUP>) and P3 (with a hole mobility of 0.2 cm<SUP>2</SUP> V<SUP>−1</SUP> s<SUP>−1</SUP> only), P1 exhibited higher ambipolar charge carrier mobilities (1.66 cm<SUP>2</SUP> V<SUP>−1</SUP> s<SUP>−1</SUP> of holes and 0.05 cm<SUP>2</SUP> V<SUP>−1</SUP> s<SUP>−1</SUP> of electrons).</P></▼2>

D–A copolymer with high ambipolar mobilities based on dithienothiophene and diketopyrrolopyrrole for polymer solar cells and organic field-effect transistors

Kim, In-Bok,Khim, Dongyoon,Jang, Soo-Young,Kim, Jihong,Yu, Byung-kwan,Kim, Yeong-A,Kim, Dong-Yu Elsevier 2015 ORGANIC ELECTRONICS Vol.26 No.-

<P><B>Abstract</B></P> <P>Donor–acceptor (D–A) type conjugated polymers have been developed to absorb longer wavelength light in polymer solar cells (PSCs) and to achieve a high charge carrier mobility in organic field-effect transistors (OFETs). PDTDP, containing dithienothiophene (DTT) as the electron donor and diketopyrrolopyrrole (DPP) as the electron acceptor, was synthesized by stille polycondensation in order to achieve the advantages of D–A type conjugated polymers. The polymer showed optical band gaps of 1.44 and 1.42eV in solution and in film, respectively, and a HOMO level of 5.09eV. PDTDP and PC<SUB>71</SUB>BM blends with 1,8-diiodooctane (DIO) exhibited improved performance in PSCs with a power conversion efficiency (PCE) of 4.45% under AM 1.5G irradiation. By investigating transmission electron microscopy (TEM), atomic force microscopy (AFM), and the light intensity dependence of <I>J</I> <SUB>SC</SUB> and <I>V</I> <SUB>OC</SUB>, we conclude that DIO acts as a processing additive that helps to form a nanoscale phase separation between donor and acceptor, resulting in an enhancement of <I>μ</I> <SUB>h</SUB> and <I>μ</I> <SUB>e</SUB>, which affects the <I>J</I> <SUB>SC</SUB>, EQE, and PCE of PSCs. The charge carrier mobilities of PDTDP in OFETs were also investigated at various annealing temperatures and the polymer exhibited the highest hole and electron mobilities of 2.53cm<SUP>2</SUP> V<SUP>−1</SUP> s<SUP>−1</SUP> at 250°C and 0.36cm<SUP>2</SUP> V<SUP>−1</SUP> s<SUP>−1</SUP> at 310°C, respectively. XRD and AFM results demonstrated that the thermal annealing temperature had a critical effect on the changes in the crystallinity and morphology of the polymer. The low-voltage device was fabricated using high-<I>k</I> dielectric, P(VDF-TrFE) and P(VDF-TrFE-CTFE), and the carrier mobility of PDTDP was reached 0.1cm<SUP>2</SUP> V<SUP>−1</SUP> s<SUP>−1</SUP> at <I>V</I> <SUB>d</SUB> =−5V. PDTDP complementary inverters were fabricated, and the high ambipolar characteristics of the polymer resulted in an output voltage gain of more than 25.</P> <P><B>Highlights</B></P> <P> <UL> <LI> We describe the synthesis and characterizations for donor–acceptor copolymer, PDTDP. </LI> <LI> PDTDP shows high crystallinity and appropriate energy levels for ambipolar OFETs. </LI> <LI> We achieved a 4.45% efficiency with processing additive, DIO, in PSCs. </LI> <LI> We achieve ambipolar field effect mobilities, <I>μ</I> <SUB>h</SUB> ∼2.53 and <I>μ</I> <SUB>e</SUB> ∼0.36cm<SUP>2</SUP> V<SUP>−1</SUP> s<SUP>−1</SUP>. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>

연속 혈당 측정 데이터를 이용한 딥러닝 기반 저혈당 예측 모델

김현아(Hyun-A Kim),문지은(Ji-Eun Moon),윤영석(Yeong-Seok Yun),이현지(Hyeon-Ji Lee),장혜원(Hye-Won Jang),이혁준(Hyuk-Jun Lee),엄태현(Tae-Hyeon Um),이재선(Jae-Seon Lee),이승천(Seung-Chun Lee) 한국정보과학회 2021 한국정보과학회 학술발표논문집 Vol.2021 No.6