Disorders of Liver : The Efficacy Of Hepatic Resection After Preoperative Chemoembolization And Radiation Therapy In Hepatocellular Carcinoma More Than 5 cm In Size

( Sae Byeol Chol ),( Kyung Sik Kim ),( Dong Sub Yoon ),( Kwang Hyub Han ),( Woo Jung Lee ),( Byong Ro Kim ),( Jin Sil Seong ),( Jong Tae Lee ) 대한소화기학회 2007 SIDDS Vol.9 No.-

Background/Aims: In cases of advanced hepatocellular carcinoma (HCC), palliative treatment such as transarterial chemoembolization or chemoinfusion (TACE/TACI) and radiation therapy are performed preoperatively. The aim of this study was to evaluate the outcome of these preoperative treatment prior to hepatic resection. Methods: Between January 1994 and May 2007, 16 patients with advanced stage HCC greater than 5 cm in size are treated with TACE/TACI and radiation therapy prior to hepatic resection. The clinicopathologic factors were reviewed retrospectively. Results: Of the sixteen patients, there were fourteen men and two women, and the mean age was 51 years (range 22-70 years). Preoperative TACE/TACI was performed three times in average, and the median radiation dosage was 45 Gy (range 30-68 Gy). Major hepatic resection was performed. The median diameter of tumor on specimen was 9.0 cm. The degree of tumor necrosis was more than 90% in fourteen patients. Median survival time was 13.3 months. Eleven patients were dead due to tumor recurrence (n=8) and hepatic failure (n=2). Five patients had survived more than 2 years and there were two patients who had survived more than 5 years. Conclusions: Although the prognosis of HCC patients who were underwent preoperative combined TACE/TACI and radiation therapy is not satisfactory, some showed long term survival more than 5 years. Further research will be required to examine the survival and disease control impact in a prospective randomized study.

Radical Cation-Anion Coupling-Induced Work Function Tunability in Anionic Conjugated Polyelectrolytes

Lee, Jong-Hoon,Lee, Byoung Hoon,Jeong, Song Yi,Park, Sae Byeol,Kim, Geunjin,Lee, Seoung Ho,Lee, Kwanghee Wiley Blackwell (John Wiley Sons) 2015 ADVANCED ENERGY MATERIALS Vol.5 No.23

Free Paper Session : Liver 1 ; Central Bisectionectomy-A Strategy For The Preservation Of Liver Volume In Centrally Located Hepatocellular Carcinoma

( Sae Byeol Choi ),( Jae Gil Lee ),( Kyung Sik Kim ),( Jin Sub Choi ),( Woo Jung Lee ),( Byong Ro Kim ) 대한소화기학회 2007 SIDDS Vol.9 No.-

Background/Aims: Central bisectionectomy which desecribes the removal of the central hepatic segments (IVA, IVB, V, VIII) for hepatocellular carcinoma (HCC) is performed to reduce the volume of the resected liver and to overcome insufficient future residnal volume. Methods: 27 patients with HCC underwent central bisectionectomy from January 1998 to April 2007 in Severance hospital. The surgical techniques, clinicopathological characteristics and outcomes were reviewed. Results: The clinicopathological characteristics are shown in Table 1. The median operation time was 330 minutes. The postoperative complications developed in twelve patients, and mortality was occurred in two patients. The most common complication, present in five patients. Was bile duct injury leading to biloma or bile leakage. Median follow up period was 19.1 months (1.4~102.2 months) and eight patients had recurrences. 24 patients are alive. Conclusions: Although biliary complications occur somewhat frequently. Central bisectionectomy in centrally located HCC can be performed safely to preserve liver volume. And the oncologic outcomes will be further evaluated with accumulation of the experiences.

Comparative Whole Genome Transcriptome Analysis of Rice Stripe Virus Viruliferous and Non Viruliferous Small Brown Planthopper, Laodelphax striatellus

Saes Byeol An,Jae Young Choi,Seok Hee Lee,Ying Fang,Jong Hoon Kim,Seung Hee Lee,Shin Sang Woon,Woo Jin Kim,Yeon Ho Je 한국응용곤충학회 2014 한국응용곤충학회 학술대회논문집 Vol.2014 No.04

Rice stripe virus (RSV) is one of the serious plant pathogenic viruses for rice and mediated by small brown planthopper, Laodalphax striatellus. So far, the studies have been mainly focused on the interaction between the host plant and the virus. In this study, for better comprehension of the interactions among Rice stripe virus, rice and small brown planthopper, transcriptomes of the RSV-viruliferous (RVLS) and non-viruliferous L. striatellus (NVLS) were comparatively analysed. For this, non-viruliferous L. striatellus were collected from non-infected rice field and fed RSV-infected rice for 5 days. With the RNAs prepared from the RSV-viruliferous and the non-viruliferous small brown planthoppers, we conducted Illumina RNA sequencing (Hiseq 2000) and then two transcriptome databases were generated from RVLS and NVLS, respectively. The transcriptome of RVLS and NVLS were campared to figure out how the gene expression of the insects affected by Rice Stripe Virus. RSV-dependently regulated genes analysed from this study may have important functions in the transmission and replication of RSV.

예비유아교사가 인식하는 유아영재교육의 의미

이새별(Sae Byeol Lee),황희숙(Hee Sook Hwang) 한국영재학회 2018 영재교육연구 Vol.28 No.2

본 연구는 예비유아교사를 대상으로 유아영재교육에 대한 메타포를 분석하고자 하였다. 이를 위해 부산, 경남에 소재하고 있는 3, 4년제 대학의 유아교육과에 재학 중인 대학생 213명을 대상으로 질문지를 통해 자료를 수집하였다. 수집된 자료는 체계적 은유분석을 바탕으로 이루어졌다. 연구결과, 예비유아교사들이 인식하는 유아영재교육은 유아영재교육의 필요성, 유아영재교육의 목적, 교수학습방법, 유아영재 교사교육, 교사의 역량인 5개의 대분류와 긍정적인 시각, 부정적인 시각, 개인의 잠재력 발견 및 자아실현, 미래사회에 기여하는 인재 양성, 속진 및 심화교육, 개인적인 독특성을 고려한 교육, 생소하고 애매한 교육, 유아교사가 배워야 할 교육, 유아영재교사의 자질인 9개의 내용유형이 도출되었다. 본 연구 결과는 유아영재교육에 대한 교사교육을 위한 기초자료로 활용할 수 있을 것이다. This study investigated pre-service early childhood teachers’ metaphor of gifted young children education. From 213 pre-service teachers, 181 metaphors were identified through metaphor analysis, and nine categories symbolizing the prospective teachers’ understanding of gifted young children education were identified. According to the results, 28.7% of the participants conceived gifted young children education as the discovery of individual potential and self-realization. The other metaphors were negative viewpoint(20.5), the qualities of a gifted teacher(14.4%), human resources development(8.8%), curriculum for early childhood teachers(8.8%), unfamiliar and imprecise education(7.2%), positive viewpoint(5.5%), enrichment and acceleration(5.0%), and individual differences in education(1.1%). The results were discussed with relation to implications for pre-service early childhood teacher education.

어린이집의 조직문화와 보육교사의 창의적 인성이 보육교사의 행복에 미치는 영향

이새별 ( Sae Byeol Lee ),황희숙 ( Hee Sook Hwang ) 미래유아교육학회 2016 미래유아교육학회지 Vol.23 No.2

본 연구는 보육교사가 인식한 어린이집의 조직문화, 보육교사의 창의적 인성이 보육교사의 행복에 미치는 영향을 알아보고자 하였다. 이를 위해 보육교사 261명을 대상으로 설문조사를 실시하였고, 자료처리는 SPSS 22.0 프로그램을 사용하여 Pearson의 적률상관분석과 위계적 중다회귀분석을 실시하였다. 연구결과는 첫째, 보육교사의 어린이집의 조직문화에 대한 인식 정도는 집단문화, 개발문화, 위계문화, 합리문화 순으로 나타났고, 창의적 인성, 행복에 대한 인식 정도는 보통보다 높은 수준인것으로 나타났다. 둘째, 어린이집의 조직문화 중 집단문화와 개발문화, 보육교사의 창의적 인성은 행복과 정적 상관관계가 있는 것으로 나타났다. 셋째, 보육교사의 행복에 미치는 상대적 영향력을 살펴본 결과 어린이집의 조직문화 중 집단문화와 보육교사의 창의적 인성 중 호기심은 보육교사의 행복을 25% 설명하는 것으로 나타났다. 이러한 결과는 보육교사의 행복 증진을 위해 교사의 호기심을 함양할 수 있는 교육기회와 지원이 필요하며 이와 더불어 상호친화적인 어린이집의 조직문화를 형성하기 위한 프로그램 개발 및 교직원들 간의 노력이 필요함을 시사한다. The purpose of this study was to investigate the relationship among organizational culture of a daycare center, creative personality and happiness. The participants of this study were 261 daycare teachers. They completed the survey questionnaire composed of organizational culture of a daycare center scale, creative personality scale and happiness scale. The collected data were analyzed by descriptive statistics, Cronbach’s alpha, Pearson’s correlation, and multiple regression. The results were as follows: First, there were significant relations among group culture, development culture, creative personality and happiness. Second, group culture, rational culture and creative personality affected happiness of daycare teachers. From the findings, it can be suggested that organizational culture of a daycare center and creative personality are critical variables to improve happiness of daycare teachers.

Comparative Transcriptome Analysis of Queen, Worker, and Larva of Asian Honeybee, Apis cerana

Seok Hee Lee,Sang Woon Shin,Saes Byeol An,Fang Ying,Jong Hoon Kim,Seung Hee Lee,Jae Young Choi,Woo Jin Kim,Yeon Ho Je 한국응용곤충학회 2014 한국응용곤충학회 학술대회논문집 Vol.2014 No.04

The Asian honeybee, Apis cerana, is a native honeybee species in Korea which is important in agriculture for pollination and honey production. For better understanding of the physiology of A. cerana, high-throughput Illumina transcriptome sequencing was performed to analyze the gene expression profiles of queen, worker and larva. A total of 219,799,682 clean reads corresponding to 22.2Gb of nucleotide sequences was obtained from the whole body total RNA samples. The Apis mellifera reference mRNA sequence database was used to measure the gene expression level with Bowtie2 and eXpress software and the Illumina short reads were mapped to 11,459 out of 11,736 A. mellifera reference genes. Total of 9,221 genes with FPKM value greater than 5 of each sample group were subjected to evolutionary genealogy of genes: Non-supervised Orthologous Groups (eggNOG) with BLASTX for gene ontology analysis. The differential gene expression between queen and worker, and worker and larva were analyzed to screen the overexpressed genes in each sample group. in the queen and worker sample group, total of 1,766 genes were differentially expressed with 887 and 879 genes overexpressed over two folds in queen and worker, respectively. In the worker and larva sample group, total of 1,410 genes were differentially expressed with 1,009 and 401 genes overexpressed over two folds in worker and larva, respectively.

Discovery of Plant Antagonists of Insect Juvenile Hormone

Seok-Hee Lee,Ying Fang,Saes-Byeol An,Doo-Sang Park,Hyuk-Hwan Song,Sei-Ryang Oh,Soo-Young Kim,Seonghyun Kim,Namjung Kim,Hyun-Woo Oh,Yeon Ho Je,Sang Woon Shin 한국응용곤충학회 2014 한국응용곤충학회 학술대회논문집 Vol.2014 No.10

Insects impact human health through vector-borne diseases and cause major economic loses through damaging crops and stored agricultural products. Insect-specific growth regulators (IGR) represent attractive control agents because of their safety to the environment and humans. Here, we report identification of plant compounds that are antagonists of the insect-specific juvenile hormone (PJHANs), using the yeast two hybrid system transformed with the mosquito JH receptor as a reporter assay. We show that these compounds act by inhibiting larval growth and reproduction in mosquitoes. We also demonstrate that PJHANs affect the JH receptor, Methoprene-tolerant (Met), by disrupting its complex with CYCLE, formation of which is required for mediating JH action. We isolated five diterpene secondary metabolites with JH antagonist activity from two plants, Lindera erythrocarpa and Solidago serotina. They are effective in causing mortality of mosquito larvae at relatively low LD50 values. Two of these diterprenes affect Met function, leading to reduction in expression of Met target genes and causing retardation of follicle development in mosquito ovaries. Developing potent compounds counteracting JHaction (JH antagonists) would find a wider range of control applications. However, so far such JH antagonists have not been developed. Here, we report the discovery of potent JH antagonists in plants, which represents an innate resistance mechanism of plants against insect herbivores. These newly discovered plant JH antagonist compounds could be used as the starting material for developing novel insecticides.

Molecular Biological Characterization of the Mosquitocidal Bacillus thuringiensis Strain, serovar mogi

Qin Liu,Jae Young Choi,Seok Hee Lee,Saes Byeol An,Song Eun Kim,Woo Jin Kim,Yeon Ho Je 한국응용곤충학회 2013 한국응용곤충학회 학술대회논문집 Vol.2013 No.10

The novel serogroup of Bacillus thuringiensis serovar mogi (H3a3b3d) was isolated from fallen leaves, sampled in a forest region of the city of Mungyeong, Korea. Plasmids from B. thuringiensis have been implicated in pathogenicity as they carry the genes responsible for different types of diseases in mammals and insects. In this study, the genome sequence of the strain was determined. The 6.0-Mb genome of B. thuringiensis mogi contains three replicons: a circular chromosome (5.40-Mb) encoding 5,652 predicted open reading frames (ORFs), and two megaplasmids, pMOGI364 (364 564 bp) and pMOGI222 (222 348 bp). The G+C contents of these replicons ranged from 31.3% to 34.2% for pMOGI364 and pMOGI222, respectively. There are six putative cry genes, cry19Bb1, cry73Aa, cry20Bb1, cry27Ab1, cry4Aa and cry56Ba1, distributed on these two megaplasmids. To investigate the role of these genes in crystal production, the expression profiles of these toxin genes were analyzed by quantitative PCR (qPCR) from the wild type strain. Also, these cry genes were cloned to the Escherichia coli-B. thuringiensis shuttle vector, pHT1K under the control of its own promoter and then introduced into an acrystalliferous B. thuringiensis Cry-B strain for further molecular characterization.

Identification of plant compounds that disrupt the insect juvenile hormone receptor complex

Lee, Seok-Hee,Oh, Hyun-Woo,Fang, Ying,An, Saes-Byeol,Park, Doo-Sang,Song, Hyuk-Hwan,Oh, Sei-Ryang,Kim, Soo-Young,Kim, Seonghyun,Kim, Namjung,Raikhel, Alexander S.,Je, Yeon Ho,Shin, Sang Woon National Academy of Sciences 2015 PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF Vol.112 No.6

<P><B>Significance</B></P><P>Juvenile hormone (JH) plays key roles in insect development, reproduction, and many other physiological functions. Because JH is specific to insects, it has been investigated for use as pest control. Although compounds that mimic the action of JH (JH analogues/agonists) are efficient, they have a limited scope of application. Development of potent compounds counteracting JH action (JH antagonists) would find a wider range of control applications. However, thus far, such JH antagonists have not been developed. Here, we report on the discovery of potent JH antagonists in plants, which represents an innate resistance mechanism of plants against insect herbivores. These newly discovered plant JH antagonist compounds could be used as the starting material for developing novel insecticides.</P><P>Insects impact human health through vector-borne diseases and cause major economic losses by damaging crops and stored agricultural products. Insect-specific growth regulators represent attractive control agents because of their safety to the environment and humans. We identified plant compounds that serve as juvenile hormone antagonists (PJHANs). Using the yeast two-hybrid system transformed with the mosquito JH receptor as a reporter system, we demonstrate that PJHANs affect the JH receptor, methoprene-tolerant (Met), by disrupting its complex with CYCLE or FISC, formation of which is required for mediating JH action. We isolated five diterpene secondary metabolites with JH antagonist activity from two plants: <I>Lindera erythrocarpa</I> and <I>Solidago serotina</I>. They are effective in causing mortality of mosquito larvae at relatively low LD<SUB>50</SUB> values. Topical application of two diterpenes caused reduction in the expression of Met target genes and retardation of follicle development in mosquito ovaries. Hence, the newly discovered PJHANs may lead to development of a new class of safe and effective pesticides.</P>