구두 발표 : 구두발표(OC) - 농업화학 및 생태,식품분야 ; 토양 유기물 함량에 따른 유기염소계 살충제 Endosulfan의 흡착 특성

황정인 ( Jeong In Hwang ),전영환 ( Young Hwan Jeon ),김정민 ( Jung Min Kim ),석다롱 ( Da Rong Seok ),이은향 ( Eun Hyang Lee ),전상오 ( Sang Oh Jeon ),이성은 ( Sung Eun Lee ),김장억 ( Jang Eok Kim ) 한국환경농학회 2013 한국환경농학회 학술대회집 Vol.2013 No.-

A study on fate and behavior of endosulfan, which is an organochlorine insecticide detected mainly in Korean soil, is important due to its toxicity and persistence in soil environment. To estimate isotherms for the adsorption of endosulfan isomers (α-, β-) and their metabolite (-sulfate) in soil, their adsorption amounts were investigated in various soils containing different organic matter contents. The tested soils were collected at Ansim and Gunwi, and some of them were prepared as organic matter-removed (OMR) samples using H2O2. As time-dependent adsorbed amounts of endosulfan reached to their equilibrium after 6 h in Ansim, Gunwi and OMR Ansim soils, OMR Gunwi soil did not adsorb the pesticide within 24 h. The adsorption isotherms of endosulfan isomers and their metabolite fitted to Freundlich adsorption isotherms for three equilibrated soil samples. The correlation coefficients (R2) of the isotherms were greater than 0.964. Freundlich adsorption coefficient (Kf) ranged from 8.66 to 19.20 for α-endosulfan, from 7.08 to 20.49 for β-endosulfan and from 8.62 to 17.98 for endosulfan-sulfate, respectively. The difference of Kf values of endosulfan indicated that the adsorption amount of endosulfan was dependent on the contents of organic matter. Therefore, the behavior of endosulfan in soil environment is definitely affected by the amount of organic matters in soil.

03 구두발표 : 구두발표(OA) - 농업환경 및 토양,수질 분야 ; OA-02 : 토성이 다른 토양 중 Endosulfan 이성질체의 잔류양상 예측

황정인 ( Jeong In Hwang ),전상오 ( Sang Oh Jeon ),이상협 ( Sang Hyeob Lee ),강민수 ( Min Su Kang ),정혜현 ( Hye Hyun Jung ),곽세연 ( Se Yeon Kwak ),이성은 ( Sung Eun Lee ),김장억 ( Jang Eok Kim ) 한국환경농학회 2015 한국환경농학회 학술대회집 Vol.2015 No.-

Pesticides persisted in soil environment may be subjected to the unexpected uptake by plant root. As these absorbed pesticides can result eventually in safety problems to agricultural products, it is required to predict the plant uptake extent of soil-persisting pesticides inadvance. One of the previous prediction methods is to assess the uptake extent using a mathematical model. When developing the plant uptake model of soil-persisting pesticides, adsorption and dissipation interactions between pesticides and soil may be parameters that play major roles in determining the uptake extent into plant. With considerations of parameters relative to these interactions, we developed a chemical-specific residue (CSR) model, Ce(t)=C0·(1/2)t/T/Kd, in order to predict the residual patterns of endosulfan isomers ( - and  -) in soils containing different organic matter contents. The adsorption (Kd) and dissipation (T) parameters were obtained from indoor experiments, and the applicability of CSR model was assessed by comparing between experimental measurements and modeled estimates. The Kd and T values were significantly varied depending on organic matter contents in the tested soils. For the low organic matter-containing soil, the Kd and T values of the  -isomer were 50.2 mL g-1 and 16 d, respectively; the values of the  -isomer were 54.4 mL g-1 and 231 d, respectively. On the other hand, for the high organic matter-containing soil, the Kd values were 377.4 mL g-1 for the  -isomer and 407.4 mL g-1 for the  -isomer, and the T values for the  - and  -isomers were 139 and 347 d, respectively. Using these parameter values, the modeled values were in good agreement with the measured values with standard errors of less than 23.5%. Therefore, the developed CSR model may be used as a tool for predicting the residual amount of endosulfan in soil.

LC-MS/MS를 이용한 홍삼가공품 중 잔류농약 동시분석법 개발

강민수 ( Min-su Kang ),황정인 ( Jeong-in Hwang ),이상협 ( Sang-hyeob Lee ),류준상 ( Jun-sang Ryu ),홍성현 ( Sung-hyun Hong ),정혜현 ( Hye-hyun Jung ),곽세연 ( Se-yeon Kwak ),강자군 ( Ja-gun Kang ),김호진 ( Ho-jin Kim ),김장억 ( Ja 한국환경농학회 2016 한국환경농학회 학술대회집 Vol.2016 No.-

The demand for red ginseng (RS) products made by processing ginseng has increased with an interest for health of consumer, and hence it is required to evaluate the safety for residual pesticides in the RS products. However, there are no authorized analysis methods of pesticides for RS products. In this study, a multiresidue analysis method of 43 pesticides in RS products such as pure extract and concentrated extract was developed using a liquid chromatography-tandem mass spectrometry. Small amounts of sample were used in the analysis, and the pesticides were extracted with acetonitrile. The clean-up of samples was conducted using dispersive solid phase extraction with the primary secondary amine and anhydrous magnesium sulfate. The analytical method developed in this study was amenable to 37 (pure extract) and 34 (concentrated extract) of 43 tested pesticides. Using the developed method, the limits of quantification of the amenable pesticides in the RS products were between 3 and 70 ng mL-1, and the linearities of their matrix-matched standard calibration curves were acceptable with high correlation coefficients of >0.98. In addition, recoveries of amenable pesticides in the RS products ranged from 70.0 to 118.0%, with relative standard deviations of 0.5∼19.5%. Therefore, the analytical method developed in this study could be used as an efficient analysis method of pesticides for pure extract and concentrated extract products of RS.

농산물에 대한 LC-MS/MS를 이용한 농약의 다성분분석법 확립

전영환 ( Young Hwan Jeon ),황정인 ( Jeong In Hwang ),김정민 ( Jung Min Kim ),석다롱 ( Da Rong Seok ),이은향 ( Eun Hyang Lee ),전상오 ( Sang Oh Jeon ),도정아 ( Jung Ah Do ),오재호 ( Jae Ho Oh ),홍진환 ( Jin Hwan Hong ),이영득 ( You 한국환경농학회 2013 한국환경농학회 학술대회집 Vol.2013 No.-

Pest control is major problem in front of farmer worldwide, an enormous economic loss is observed due to pest every year. There are several chemical pesticides available for controlling agricultural pests. Studies have shown chemical pesticides can cause significant health risks to humans, contaminate water supplies, and to plant itself. To come through this problem, we previously developed some natural pesticides i.e. oak pyroligneous liquor, rice bran and phytic acid which were tested against Perilla frutescens rust disease. We found good pesticide capacity of these natural extracts, so we were veracious to know whether it has toxic effect or not for that we designed the present investigation. In the present study we have determined toxicity on the basis of fish survival assay, seed germination assay and mice feed assay. Briefly, a concentration of 50 and 100μg\ml of all pesticides were supplied in water of fishes for 96hrs. We have taken two kind of fishes for this experiment Misgurnus anguillicaudatus(10) and Cyprinus carpio(10). After completion of the incubation period number of dead fish were calculated. Simultaneously, we determined seed germination test on four types of seed Cabbage, Radish, Black beans and Red kidney beans. A number of 20 seeds in case of red kidney beans 5 seeds were kept in sterile Petri dishes bedded with layer of tissue papers (Kimtec) which were wet with autoclaved distilled water. Treatment of pesticides was done with a concentration of 50 and 100μg\ml for 48 to 96 hrs. The number of non-germinated seeds was counted after the incubation period. In the case of animal toxicity test we supplied pesticides in the drinking water of mice and they were kept under investigation of body weight and death of mice. The results obtained from fish experiment suggest no toxicity of oak and rice bran but at a higher concentration one fish was found dead. In the case of seed germination assay all the seeds were observed to be germinated at both concentrations for all pesticides. No decrease in body weight was found in mice experiment as well as all mice were live. In accordance with all data, we concluded that the use of Oak, Rice bran and Phytic acid as a pesticide is safe and effective especially in the case of Perilla rust disease.

토양에 잔류된 Endosulfan의 감자에 대한 흡수 및 잔류특성

곽세연 ( Se-yeon Kwak ),황정인 ( Jeong-in Hwang ),이상협 ( Sang-hyeob Lee ),류준상 ( Jun-sang Ryu ),홍성현 ( Seong-hyeon Hong ),강민수 ( Min-su Kang ),정혜현 ( Hye-hyun Jung ),강자군 ( Ja-gun Kangand ),김장억 ( Jang-eok Kim ) 한국환경농학회 2016 한국환경농학회 학술대회집 Vol.2016 No.-

There have been reports that an organochlorine insecticide endosulfan (ED) consisted of α- and β-isomers is consistently detected in Korean arable soils due to its long-term persistency, and a major metabolite ED-sulfate produced with its degradation is as toxic and persistent as the isomers. This study assessed the uptake amount of ED isomers and ED-sulfate by potato grown on ED-treated soils with concentrations of 2 and 10 mg/kg. Soil samples were collected at 0, 3, 7, 14, 21, 30, 60, and 90 d after planting of potato, and residual amounts of total ED calculated as a sum of isomers and metabolite were halved after 62.4 and 99.5 d in 2 and 10 mg/kg-treated soils, respectively. Potato plants sampled after growth of 60 to 90 d were divided into root, shoot and tuber parts. Most of ED isomers were present in root of potato with mean distribution rate of 57.1%, while ED-sulfate was the greatest in shoot part, with mean distribution rate of 64.5%. The uptake amounts of total ED in tuber of potato were the highest after 60 d of growth, showing concentrations of 0.49-2.34 mg/kg for both treatments. In addition, the distribution rate of ED isomers in potato tubers were 2 times higher than that of ED-sulfate. In Korea, there is no maximum residue limit (MRL) for ED in potato, while Codex and EU has such MRL as 0.05 mg/kg. At the final hervest day of 90 d, uptake amounts (0.2-1.3 mg/kg) of total ED in tuber of potato exceeded the MRL. Therefore, if the potato is cultivated in contaminated soils with concentrations of >2 mg/kg, its final products may be unsafe with exceeding MRL. The results in this study may provide the useful information to establish the management guideline of ED in soil for producing the safe agricultural products.

Liquid Chromatography-Tandem Mass Spectrometry를 이용한 농산물 중 살충제 다성분 동시분석법

이상협 ( Sang-hyeob Lee ),황정인 ( Jeong-in Hwang ),강민수 ( Min-su Kang ),정혜현 ( Hye-hyun Jung ),류준상 ( Jun-sang Ryu ),홍성현 ( Sung-hyun Hong ),곽세연 ( Se-yeon Kwak ),강자군 ( Ja-gun Kang ),도정아 ( Jung-ah Do ),장문익 ( Mo 한국환경농학회 2016 한국환경농학회 학술대회집 Vol.2016 No.-

For regulating pesticide residues in agricultural products imported from other countries, Korea is ready to introduce the Positive List System permitting the determination of unregistered pesticides in them at a concentration of <0.01 mg/kg. Hence, it is required to establish a rapid and sensitive analysis method for both registered and unregistered pesticides in imported agricultural products, in order to ensure their safety from pesticide residues. In this study, we developed an analytical method for 62 insecticides in three agricultural products such as orange, green pepper, and brown rice using liquid chromatography-tandem mass spectrometry. Acetonitrile and sodium citrate were used for the extraction of insecticide residues from the samples, and a dispersive solid phase extraction using primary secondary amine and anhydrous magnesium sulfate was employed for a clean-up. Using the developed method, the limits of quantification were below 0.01 mg/kg for all tested insecticides, and their matrix-matched standard calibration curve had high correlation coefficients of >0.99. In addition, for all tested samples, recoveries of insecticides fortified at levels of 0.01-0.05 mg/kg ranged from 75.2 to 111.3%, and relative standard deviations were acceptable at <18.1%. Therefore, the analytical method developed in this study could be used as the rapid and sensitive analysis method for 62 insecticides in agricultural products.

살균제 Fludioxonil 및 Metconazole의 밀 부위별 잔류 양상

강자군 ( Ja-gun Kang ),황정인 ( Jeong-in Hwang ),이상협 ( Sang-hyeob Lee ),강민수 ( Min-su Kang ),정혜현 ( Hye-hyun Jung ),곽세연 ( Se-yeon Kwak ),류준상 ( Jun-sang Ryu ),홍성현 ( Seong-hyeon Hong ),박준홍 ( Jun-hong Park ),김장억 한국환경농학회 2016 한국환경농학회 학술대회집 Vol.2016 No.-

Residual patterns of fungicides fludioxonil and metconazole applied on wheat with their safe use guidelines were investigated with consideration for their distribution rates in each compartment of wheat. The fungicides were 2 or 3 times sprayed on wheats at the interval of 7 days prior to harvest. Wheat samples collected at final harvest day were divided into three compartments such as grain, hull and straw, and the pesticide residue analysis was individually carried out to such compartments of wheat. The analytical methods of fungicide residues in wheat were acceptable, with recoveries of 84.3 to 113.3% and correlation coefficients of 0.1 to 4.7%. Due to the systemic characteristics, the residual amounts of metconazole in the grain part of wheat were greater as 0.11~0.29 mg/kg than those amounts (0.02~0.07 mg/kg) for fludioxonil, and these residual amounts of both fungicides exceeded their maximum residue limits. According to absolute residue amounts of the fungicides in one wheat plant, their distribution rates in each compartment of wheat were the highest in straws (66.7~70.7%), followed by hulls (29.0~32.5%) and grains (0.2~0.9%). Although the distribution rates of fludioxonil and metconazole in wheat were the lowest in the edible grain parts, their high distribution rates in hulls and straws, available as feeds for livestock, may result in safety problems by pesticide residues. Therefore, it is required to assess the residual characteristics of pesticides in individual compartments of crops in order to ensure the safety for either foods to human or feeds to livestock from their residues.

연구보문 : 환경화학 ; 한약재 진피에 대한 유기인계 살충제의 잠정 MRL 설정 및 안전사용기준 제안

이주희 ( Ju Hee Lee ),신갑식 ( Kab Sik Shin ),전영환 ( Young Hwan Jeon ),김효영 ( Hyo Young Kim ),황정인 ( Jeong In Hwang ),이병희 ( Byung Hee Lee ),강인호 ( In Hoo Kang ),강신정 ( Shin Jung Kang ),김태화 ( Tae Hwa Kim ),김장억 ( 한국환경농학회 2010 한국환경농학회지 Vol.29 No.1

Jinpi(Aurantii nobilis Pericarpium) is one of the most important material of oriental herbal medicine which is made from the peel of mandarin by washing with hot water and drying. Pesticides have necessarily used for mandarin cultivation according to their preharvest intervals (PHIs) but their maximum residue limits (MRLs) and PHIs for Jinpi are not established yet. This study is to know residue amount of organophosphorus insecticides in Jinpi and to establish the MRLs and PHIs for fenitrothion and phenthoate in Jinpi. Fenitrothion was sprayed once, twice and three times with 7 days interval before harvest. Its residue amount ranged from 0.14 to 1.17 mg/kg in mandarin, 0.59 to 4.02 mg/kg in its peel and 1.66 to 22.38 mg/kg in Jinpi. In case of phenthoate, it was sprayed with 10 days interval for 10 days before harvest. Its residue amounts in mandarin, its peel, and Jinpi ranged from 0.16 to 0.65, 0.69 to 2.41 and 1.69 to 11.3 mg/kg, repectively. Proposed MRLs of fenitrothion and phenthoate for Jinpi are suggested to 22.39 and 11.30 mg/kg, respectively. So we recommend PHIs of the pesticides that fenitrothion can be sprayed 3 times 7 days before harvest and in case of phenthoate, sprayed 3 times 10 days before harvest.

국가직무능력표준(NCS) 커피교육과정 SWOT분석

정인성 ( Jung In-sung ),황규범 ( Hwang Gyu-beom ) 한국융합과학회 2018 한국융합과학회지 Vol.7 No.3

연구목적: 본 연구는 국가직무능력표준(NCS) 커피교육과정 SWOT분석을 통해 현재 커피산업 분야의 교육적인 측면의 동향을 파악하는데 연구의 주된 목적이 있다. 연구방법: 본 연구는 2017년 7월 1 일~7월 30일까지 약 30일 동안 9명의 커피산업에 종사하고 있는 전문가들을 대상으로 SWOT기법을 적용한 질문지법과 심층면담기법을 활용하여 수행되었다. 결론: 결론은 다음과 같다. 첫째, NCS 강점요인은 교육비 지원과 취업 및 재취업의 부담감소, 전문 인력 개발 및 인프라 구성에 유용한 체계화된 교육, 교육과정 이수 후 강점요인은 커피산업 현장 전문가 양성, 장기간 체계적 교육 및 현장 실무위주 실습, 다양한 분야 학습, 교육과정 이수 후에 대한 비전으로는 전문적 지식을 가진 바리스타들이 현재 및 향 후 커피산업의 발전에 지속적인 영향을 미칠 것으로 전망된다. 둘째, NCS 약점요인은 커피산업 종사자 대부분이 NCS 교육과정에 대한 인식이 부족, 빠르게 변하고 있는 커피 커피산업의 트랜드를 충족시키지 못함, 교육의 질 저하와 함께 이론적 내용의 부족함, 현장실습 교육 필요함 등으로 나타났다. 셋째, NCS 위협요인은 종사자의 낮은 연령과 피교육자의 연령이 높을 때는 별다른 교육과정 없이 현장에 투입됨으로써 진입장벽이 현저히 낮다. 특히, 짧은 기간에 이루어지는 주입식 교육, 낮은 교육수준, 변화를 따라가지 못하는 교육 등이다. 넷째, NCS 기회요인은 현장중심으로 체계화된 교육과정을 통한 전문가 양성, 질 높은 평생교육기회 부여, 이수자들의 체계적인 경력 및 인력관리 현장중심 교육과정의 업데이트, 현장중심의 실무교육, 교육·훈련·자격·연계교육 시스템 적용이 필요하다. Pupose: This study aims to figure out a trend of an educational aspect of the coffee industry by the SWOT analysis of a National Competency Standards (NCS) education of the coffee. Method: This study was done by surveys and interviews which are implied SWOT with nine coffee specialists from 1st to 30th, July 2017. Conclusion: First, the strengths of NCS are supporting education costs, decreasing burdens of finding and re-finding jobs, and systemized education for nurturing professionals and building infrastructure; the strengths of post-education are training experts, long-term systemized education and work experience, and diverse education. The vision of post-education are predicted the baristas equipped professional knowledge affect the present and future development of the coffee industry. Second, a lack of awareness about NCS education of most workers at the coffee industry, fulfilling not a trend of the coffee industry changing rapidly, decreasing the quality of the education and lack of theory, and the needs of field experience are the weaknesses of NCS. Third, low entry barriers, that an aged trainee with a young worker would work on the field without some educational processes, are threat of NCS. Especially, learning by rote within short period, low educational levels, and educational process that does not fit in the trend are the threats of NCS. Fourth, the opportunities of NCS are training experts with systemized educational process in the middle of the filed, whole-life education of high-quality, systematic managing career and job capacity of the trainees. For this, these are needed such as update of field education, real work experience on the field, related education system of education, training, and qualifying.

전투기급 항공기의 흡입구 성능 분석을 위한 풍동시험 기법

황배근(Bae-Geun Hwang),정인면(In-Myun Jung),이상수(Sang-Soo Lee),박시영(Si-Young Park),노현우(Hyun-Woo Roh) 한국항공우주학회 2020 한국항공우주학회 학술발표회 논문집 Vol.2020 No.7