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The Microbial Population in the Air of Cultivation Facility of Oyster Mushrooms
천세철,안유나,Sajid Mohamad Khan,정일민,원향연,전창성,박율진 한국미생물학회 2012 The journal of microbiology Vol.50 No.6
The microbial population in the air of mushroom cultivation facility was studied to understand the population structure and size depending on the cultivation methods and regions. The air contents of ten farmers’ oyster mushroom cultivation facilities in Kyunggi province were sampled. The results indicated that there was no difference in population size depending on the regions of mushroom cultivation. In addition, the population size of bacteria in the growth room was bigger than that of the cooling room and outside of the mushroom house, but the fungal population was similar in size between cultivation stages. With regard to population structure, Pseudomonas and Penicillium species were most frequently isolated from the air of oyster mushroom cultivation facility.
Se-Chul Chun(천세철),Mung Hwa Yoo(류명화),Young Sook Moon(문영숙),Mi Ho Shin(신미호),Ki-Cheol Son(손기철),Ill-Min Chung(정일민),Stanley J. Kays 한국원예학회 2010 원예과학기술지 Vol.28 No.3
실내식물 9종의 근권부 하이드볼 배지에서 배양된 세균집단이 공기중 벤젠과 같은 휘발성 유기화합물의 제거효과에 미치는 영향이 조사되었다. 여러 식물 근권부의 배양토에서 배양된 세균 집단은 벤젠을 제거할 수 있었는데, Spathiphyllum wallisii Regel, Pachira aquatica, Ficus elastica, Dieffenbachia sp. ‘arrianne’Hort., Chamaedorea elegans 식물들은 벤젠의 초기농도를 1.000으로 기준하였을 때, 세균 집단이 전혀 없는 배지의 대조구 초기 농도 대비 잔류율이 0.596이었으나 상기 언급한 식물들은 0.741-1.000으로서 벤젠의 농도를 현저히 감소시켰다(LSD, P=0.05). 이와 같은 경향은 식물의 종류에 따라 차이는 있었지만 톨루엔의 경우에도 비슷하게 나타났다. 이러한 결과를 바탕으로 P. aquatic 근권부의 배양토로부터 배양된 세균 집단을 P. aquatica, F. elastica, S. podophyllum에 접종하였을 때 접종하지 않은 식물들에 비하여 벤젠과 톨루엔을 현저히 제거하는 효과가 나타나, 근권부의 미생물 집단을 이용하여 공기 중 휘발성 유기화합물(VOC)을 제거할 수 있음을 보여 주었다. Total bacterial populations were cultured from the Hydroball cultivation media in the rhizospheres of 9 different plants including Hedera helix L. and Dracaena deremensis cv. Warneckii Compacta, etc. These cultured bacterial populations were studied to test if the bacterial populations in the plant growing pots may play a role on removal of volatile organic compounds (VOCs) such as benzene and toluene in the air. To meet this objective, first, we tested the possibility of removal of VOCs by the cultured total bacteria alone. The residual rates of benzene by the inoculation of total bacterial populations from the different plant growth media were significantly different, ranging from 0.741-1.000 of Spathiphyllum wallisii ‘Regal’, Pachira aquatica, Ficus elastica, Dieffenbachia sp. ‘Marrianne’ Hort., Chamaedorea elegans, compared to the control with residual rate of 0.596 (LSD, P=0.05). This trend was also similar with toluene, depending on different plants. Based on these results, we inoculated the bacterial population cultured from P. aquatica into the plant-growing pots of P. aquatica, F. elastica, and S. podophyllum inside the chamber followed by the VOCs injection. The inoculated bacteria had significant effect on the removal of benzene and toluene, compared to the removal efficacy by the plants without inoculation, indicating that microbes in the rhizosphere could play a significant role on the removal of VOCs along with plants.
Chun, Se-Chul,Lee, Keumhee,Song, Y. S.,Ko, K. I.,Kim, B. K. 建國大學校附設農業資源開發硏究所 2002 農資源開發論集 Vol.24 No.-
박과 종자로부터 효율적인 오이녹반모자이크 바이러스(CGMMV) 추출방법을 ELISA에 의하여 조사 비교하였다. 종자를 30분에서 48시간 바이러스 추출 완충 용액에 침지하여 추출한 방법, 2시간 침지 후 2시간 냉동시키고 해동하여 10시간 동안 방치하여 추출한 방법을, 종자를 분쇄하여 추출하는 방법과 비교 평가하였다. 14시간 침지에 의한 추출과, 2시간 침지 후 2시간 냉동, 10 해동 방치 추출한 것은 분쇄법에 의한 추출법과 ELISA의 CGMMV 검출 효율이 유사하였다. 따라서, 노동이 많이 소요되는 분쇄법 대신에 14시간 침지 누출이나, 2시간 침지 후 2시간 냉동, 10시간 해동 방치하는 추출법을 사용할 수 있을 것임을 제시하여 주었다. 또한, 마이크로플레이트의 한 well 당 종자의 양을 1개보다는 10, 20개 사용하는 것이 검출효율이 유사하였다. 이는 총 사용되는 종자의 양이 일정하다고 할지라도, well 당 사용되는 종자가 20개일 경우까지는 well 당 사용되는 종자를 수를 증가시키더라도 바이러스를 검출할 효율은 동일함을 제시하여 주었다. Efficient methods of virus extraction from gourd seeds for the detection of enzyme-linked immnosorbent assay (ELISA) were compared. Seeds were soaked in sample extraction buffer (Agdia, Inc., Elkhart, IN USA) for 30min∼48hrs to exude cucumber green mottle mosaic virus. In addition, 2 hr soaking in sample extraction buffer and 2 hr of freezing followed by 10 hr thawing extract (2S-2F-10T) was used for the detection of the virus by ELISA. Whole seed and seed coat only were extracted for the detection of cucumber green mottle mosaic virus (CGMMV) by ELISA. Also, the effect of amount of seeds on the rate of detection of the virus by ELISA was studied. Soaking seeds for 14hr and 2S-2F-10T methods were similar to seed and only seed coat crushing methods on the efficiency of virus detection, indicating that seed soaking methods for the extraction could be used instead of mechanical breaking seeds with similar detection rate of the virus from gourd seeds. The results suggested that the chance of detection for the virus by increasing amount of seeds up to 20 seeds per well culd be same to one seed per well, although total amount of seeds for detection of the virus would be same for one seed and 20 seeds per well.
Fusarium spp.에 의한 호접란과 풍란류에 발생하는 뿌리 및 줄기기부썩음병
김진원,천세철 한국식물병리학회 2007 식물병연구 Vol.13 No.1
Root and basal stem rot disease occurred on moth orchid (Phalaenopsis spp.), Pung-nan (Neofinetia falcata) and Nadopung-nan (Aerides japonicum) grown in the farmers' greenhouses located in Namyangju Kyonggi province, Korea during 2005 to 2006. Wilting symptoms occurred on these orchard plants at initial stage and the infected plant leaves turned yellow to red. The discolored leaves were fallen down to lead to eventual death of the entire plant. A total of 59 isolates of Fusarium spp. was obtained from roots and leaf bases of the diseased plants. The cultural and morphological characteristics of isolated Fusairum spp. were identified as Fusarium oxysporum, F. proliferatum and F. solani. F. oxysporum and F. proliferatum were isolated from all these orchard plants but F. solani was isolated only from Phalaenopsis spp. Pathogenicity of the three Fusarium spp. was confirmed by artificial inoculation. Although F. oxysporum, F. proliferatum and F. solani causing root rot disease in Phalaenopsis spp. have been reported in Korea, the pathogens in N. falcata and A. japonicum were not reported yet. Therefore, this is the first report on the root and stem rot of N. falcata and A. japonicum caused by F. oxysporum and F. proliferatum in Korea.