흰쥐 대장 배상세포내 점액질에 미치는 유기인제 농약 PAP의 영향에 관한 조직화학적 연구

조운복 부산대학교 1987 자연과학논문집 Vol.43 No.-

유기인제인 PAP살충제가 근위결장, 원위결장 및 직장 배상세포내 점액질 생성과 분비에 미치는 영향을 구명하기 위하여 성숙한 흰쥐에 PAP살충제를 경구적으로 투여하여 중독시킨 후 1시간군, 3시간군, 6시간군, 12시간군, 24시간군, 48시간군 및 72시간군 별로 나누어 배상세포내 점액질을 alcian blue pH2.5 염색, alcian blue pH 1.0 염색, alcian blue pH 0.4 염색, PAS반응, alcian blue pH 2.5-PAS 염색, alcian blue pH1.0-PAS 염색, aldehyde fuchsin pH 1.7-alcian blue pH 2.5 염색 및 high iron diamine-alcian blue pH 2.5 염색으로 관찰하였다. 농약투여 후 대장 배상세포내 점액질 감량은 표면 및 은와저부 쪽으로 은와상부보다 더 심하였으며 근위결장에서는 산성점액질과 중성점액질의 감량이 비슷하였으나 원위결장 및 직장에서는 중성점액질의 감량이 산성점액질 감량보다 더 심하였다. 장기별로는 근위결장 배상세포가 제일 심한 영향을 받았고 다음이 원위결장 및 직장 배상세포 순이었다. 대장 은와상부내 강 sulfomucin을 분비하는 배상세포가 농약투여 후 감소되었으나 sialomucin을 분비하는 배상세포가 증가되었고 근위결장에서 제일 심하게 나타났다. 대장 은와저부내 강 sulfomucin을 분비하는 배상세포와 sialomucin을 분비하는 배상세포가 농약투여 후 다같이 그 염색성이 감소되었으며 전자는 후자보다 더 심한 영향을 받았다. This histochemical study was performed to observe the effect of the organophosphate pesticide PAP on the production and secretion of mucosubstances of the goblet cells in the proximal colon, distal colon and rectum. Adult rats were administered PAP(57mg/kg), orally and sacrificed in 1,3,6,12,24,48 and 72 hours after PAP administration. The mucosubstances were stained with alcian blue pH 2.5, alcian blue pH 1.0, alcian blue pH 0.4, periodic acid Schiff(PAS), alcian blue pH 2.5-PAS, alcian blue pH 1.0-PAS, aldehyde fuchsin pH 1.7-alcian blue pH 2.5 and high iron diamine-alcian blue pH 2.5 methods. Decrease of acid and neutral mucins was noted to be considerable in the goblet cells of the large intestine, especially of the surface and basal regions of the crypts. Decrease of acid and neutral mucins was similar in the proximal colon, but the neutral mucins became more markedly diminished than acid ones in the distal colon and rectum. According to the organs, the goblet cells of proximal colon were severely affected, next those of the distal colon and rectum were affected by PAP. The number of goblet cells secreting strongly sulfomucins tended to decrease in the upper regions of the crypts of large intestine by PAP, while the number of those secreting sialomucins tended to increase. Especially, it was severely affected in the proximal colon. The stainability of the goblet cells with strongly sulfomucins and with sialomucins either in the basal regions of the crypts, generally decreased. However, a more marked decrease of the stainability appeared in the goblet cells with strongly sulfomucins than in those with sialomucins, indicating the former was markedly affected than the latter by PAP.

유기인제계 농약이 흰쥐의 대장점막내 점액질에 미치는 영향

조운복 부산대학교 1978 論文集 Vol.25 No.2

In order to study the effect of organophosphorus pesticides on the secretion and production of the mucosubstances in the large intestinal mucosa, histochemical studies were undertaken in respect of the influence of the oral administration of the DDVP, malathion, dipterex and EPN on the mucosubstances of the goblet cells in the rat colon and rectum, and traced the change brought by experimental method, namely, 3, 6, 12, 24 and 48 hour groups of administration, respectively. The mucosubstances were stained with alcian blue pH 1.0, alcian blue pH 2.5, alcian blue pH 1.0 - periodic acid Schiff (PAS), alcian blue pH 2.5 - periodic acid Schiff (PAS), aldehyde fuchsin pH 1.7 - alcian blue pH 2.5 and high iron diamine - alcian blue pH 2.5 methods. The following results were obtained. 1. It was contained large amounts of strong sulfated acid mucins and neutral mucins in the surface and upper-crypt goblet cells of the normal rat colon and rectum, but were seen mixed with most goblet cells were contained only large amounts of strong sulfated acid mucins and some were contained small amounts of nonsulfated acid mucins and neutral mucins in the lower-crypt goblet cells. 2. In administrated groups of pesticides, amounts of mucosubstances in the colic mucosa were remarkably diminished from 3hr after administration, and so were more remarkably diminished the surface and lower-crypt goblet cells than the upper-crypt goblet cells. The diminution of neutral mucins were very remarked in 3 to 6hr after dipterex and EPN administration or 6 to 12hr after DDVP and malathion administration. Amounts of neutral mucins in diminution were gradually recovered thereafter 24 to 48hr after all pesticides administration. The diminution of acid mucins were very remarked in 3 to 6hr after DDVP and EPN administration or 6 to 12hr a after malathion and dipterex administration. Amounts of acid mucins in diminution were gradually recovered thereafter 24hr after DDVP and dipterex administration or 48hr after malathion and EPN administration. Amounts of sulfated acid mucins were decreased in 3 to 12hr after administration, thereafter those were gradually increased in 24 and 48hr after administration, but amounts of nonsulfated acid mucins were some decreased in 6 to 12hr after administration, thereafter those were gradually decreased in 24 and 48hr after administration. 3. In administrated groups of pesticides, amounts of mucosubstances in the rectal mucosa were remarkably diminished from 3hr after administration, and so were more remarkably diminished the surface and lower-crypt goblet cells than the upper-crypt goblet cells. The diminution of neutral mucins were very remarked in 3 to 6hr after DDVP and EPN administration or 6 to 12hr after malathion and dipterex administration. Amounts of neutral mucins in diminution were gradually recovered thereafter 12hr after DDVP, malathion and EPN administration or 24hr after dipterex administration. The diminution of acid mucins were very remarked in 6hr after DDVP, malathion and EPN administration or 12hr after dipterex administration. Amounts of acid mucins in diminution were gradually recovered 12 to 24hr after DDVP and malathion dministration or 48hr after dipterex and EPN administration. Amounts of sulfated acid mucins were decreased in 3 to 6hr or 12hr after administration, thereafter those were gradually increased in 12 and 24hr after administration, but amounts of nonsulfated acid mucins were some decreased from 3hr after administration, thereafter those were gradually increased in 24 or 48hr after administration. On the basis of the above results, it was suggested that were severely inhibited to production and secretion of mucosubstances from earlier period after organophosphorus pesticides administration, furthermore those pesticides were more severely inhibited to production of acid mucins than neutral mucins, and so were inhibited to metabolism of sulfated ester in acid mucin, in the alteration of these mucosubstances.

관상조류 장관점막내 점액질의 성상에 관한 조직화학적 연구

조운복,송기덕 부산대학교 1980 論文集 Vol.30 No.2

This study is performed in attempt to clarify the histochemical properties and compositions of the mucosubstances in the goblet cells of the intestinal mucosa of ornamental birds. The specimens used in this study were obtained from the small and large intestines of 5 species of ornamental birds, i. e, society finch, java sparrow, zebra finch, canary and parakeet. The mucosubstances were stained with alcian blue pH 2.5, alcian blue pH 1.0, periodic acid schiff(PAS), alcian blue pH 2.5-PAS, alcian blue pH 1.0-PAS and aldehyde fuchsin pH 1.7-alcian blue pH 2.5 method. The results obtained were as follows: 1. The histochemical compositions of mucosubstances on the striated border in the intestinal mucosa, the histochemical compositions of mucosubstances of the striated border presented a refractile bilaminar appearance were different in each species. In the small intestine, the inner layer contained mostly neutral, and the outer edge mostly strong sulfated, mucins. And the former being relatively more prominent than the latter. The neutral mucins in the zebra finch, canary and parakeet contained large amounts, and those in the society finch java sparrow small amount, but the acid mucins in the java sparrow was traces, and those of the other experimental animals contained small amounts. The striated border of the large intestine was less conspicuous than that of the small intestine and contained small or large amounts of acid and small amounts of neutral mucins. The canary were equal to both neutral and sulfated mucins, but in the other experimental animals, sulfated mucins were more prominent than neutral mucins. 2. The histochemical compositions and amounts of mucosubstances in the duodenal goblet cells was in considerable variation in each species. That is, the acid mucins were more prominent than the neutral mucins in the goblet cells of duodenal mucosa of all species examined. The society finch and zebra finch contained small or large amounts of the acid mucins, and the other species examined minimal or small amounts of those. The former species amounts of acid mucins were more prominent than the latter species. In contrast, in the canary, contained small amounts of neutral mucins, were more prominent than the other species examined minimal amounts of those. In villi and crypts of the parakeet and villi of the zebra finch, were present one type of goblet cells which secrete strong sulfated mucins. But in villi and crypts of the other species examined, were present two types of goblet cells : Ones strong sulfated and nuutral mucins and others strong sulfated mucins, and the formers were much more than the latters. 3. The histochemical compositions and amounts of mucosubstances in the jejunal goblet cells was very complication and considerable variation in each species examined. That is, the acid mucins were more prominent than the neutral mucins in the goblet cells of jejunal mucosa of all species examined. The zebra finch contained small or large amounts of the acid mucins, and the other species examined small or minimal amounts of those. The former species amounts of acid mucins were more prominent than the latter species. But the zebra finch contained large amounts of the neutral mucins and the canary small amounts of those. The other species examined, on the other hand, were present minimal or small amounts of the neural mucins. In villi and crypts of the parakeet, were present one of goblet cells which secrete only strong sulfated mucins, and also in those of the canary, one type of goblet cells which strong sulfated and neutral mucins. But in villi of the java sparrow, were present two types, of goblet cells: one nonsulfated and neutral mucins and others weak sulfated and neutral ones, and the formers were much more than the letters. In crypts of the above species, were present one type of goblet cells which secrete weak sulfated and neutral mucins. In contrast, in villi of the society finch, were present two types of goblet cells: ones nonsulfated and neutral mucins and others strong sulfated and neutral ones, but in crypts of the above species, two types of goblet cells, ones strong sulfated mucins and others strong sulfated and neutral ones. The formers were much more than the latters, respectively. In villi and crypts of the zebra finch, on the others hand, were present there types of goblet cells: ones strong sulfared mucins and others strong sulfared and neutral ones, and anothers nonsulfated and neutral ones. The former two types were much more than the another types. 4. The histochemical compositions and amounts of mucosubstances in the ileal goblet cells was different in each species examined and regions. That is, the acid mucins were more prominent than the neutral mucins in the goblet cells of ileal mocosa of all species examined. The society finch and parakeet contained small or large amounts of the acid mucins, and the other species examined small or minimal amounts of those. The former species amounts of acid mucins were much more than the latter species. In contrast, the java sparrow, zebra finch and canary contained small amounts of neutral mucins and the other species examined were not demonstrated. In villi and crypts of the society finch and parkeet, were present one type of goblet cells which secrete only strong sulfated mucins, and also in those of the java sparrow and zebra finch, one type of goblet cells which strong sulfated and neutral mucins. But in those of the canary, on the other hand, were present two types of goblet cells: ones strong sulfated mucins and others strong sulfated and neutral ones. 5. The histochemical compositions and amounts of mucosubstances in the rectal goblet cells was in considerable variation in each species examined and regions. That is, the acid mucins were prominent than the neutral mucins in the goblet cells of rectal mucosa of all species examined as in the small intestine. The java sparrow, zebra finch and parakeet contained small or large amounts of the acid mucins and the other species examined small amounts of those. In contrast, the society finch and canary contained small amounts of the neutral mucins, and in the java sparrow and zebra finch minimal amounts of those, but the parakeet were not demonstrated. In surfaces and crypts of the parakeet, were demonstrated one type of goblet cells which secrete only strong sulfated mucins, and also in crypts of the society finch and java sparrow, one type of goblet cells which strong sulfated and neutral mucins. In contrast, in surfaces of the society finch, and in surfaces and crypts of the zebra finch, were present two types of goblet cells: ones strong sulfated and neutral mucins and others weak sulfated and neutral ones. The formers were much more than the latters. In surfaces of the java sparrow, were demonstrated two types of goblet cells: ones strong sulfated mucins and others strong sulfated and neutral ones.

5종 경골어류 식도점막 점액세포내 점액질의 성상에 관한 조직화학적 연구

조운복,최인장 부산대학교 1983 자연과학논문집 Vol.35 No.-

For the studying of the distribution and histochemical properties in the esophageal mucous cells of five teleostean species, i. e, Clupuadon punctatus, Parasilurus asotus, Carassius auratus, Pagrosomus major, Cantherines moderstus, we stained the esophageal mucosa of the above mentioned teleost fishes with hematoxylin-eosin, alcian blue (pH 2.5, pH 1.0 and pH 0.4), periodic acid schiff(PAS), high iron diamine(HID), alcian blue (pH 2.5 and pH 1.0) - PAS, aldehyde fuchsin pH 1.7 - alcian blue pH 2.5, and HID - alcian blue pH 2.5 methods. The results obtained were as follows: 1. The mucous cells in esophageal mucosa of Parasilurus asotus and Pagrosomus major were large in size, especially the esophageal mucous cells appeared with several layers in esophageal mucosa of all regions of esophageal fold in Pagrosomus major, but showed one layer just below surface epithelial cells in Parasilurus asotus. The mucous cells in esophageal mucosa of Carassius auratus, Canterines moderstus and Clupanodon punctatus were small in size, the mucous cells were one layer and scattering in Carassius auratus, and were seen a large numbers in the base and scattering in the top of the esophageal fold of Canterines moderstus, but were found a small numbers in only the base of esophageal fold of Clupanodon punctatus. 2. The esophageal mucous cells of both Parasilurus asotus and Cantherines moderstus were contained only neutral mucins, the former contained small amounts but the latter large or larger quantities. The esophageal mucous cells were composed of most of mucous cells with large amounts of acid and neutral mucins, and a few mucous cells with small amount of acid and neutral mucins in Clupanodon punctatus and Pagrosomus major, with most of the mucous cells being relatively more prominent of acid mucins and a few mucous cells more prominent of neutral ones. Amounts of mucins in the former contained small amounts, on the contrary the latter small amounts or large amounts. 3. According to the properties of the acid mucin, the esophageal mucous cells were composed of most mucous cells which contain large amounts of only strongly sulfated mucin and a few mucous cells having small amounts of nonsulfated mucin or weakly sulfated mucin in Pagrosomus major and Carassius auratus. However, most mucous cells in Clupanodon punctatus which contain small amounts of nonsulfated mucin or weakly sulfated mucin and a few mucous cells with small amounts of strongly sulfated mucin.

자라의 위점막내 점액질의 조직화학적 성상에 관한 연구

조운복,김봉선 부산대학교 1978 論文集 Vol.26 No.2

Six histochemical staining techniques, alcian blue pH 2.5, alcian blue pH 1.0, PAS, alcian blue pH 2.5-PAS, alcian blue pH 1.0-PAS and aldehyde pH 1.7-alcian blue pH 2.5, which differentiate neutral from sulfated and nonsulfated acid mucosubstances, were applied to section from the stomach of five terrapins (Amyda maackii Braut),to study histochemical properties of gastric mucosal mucosubstances. The results obtained were as follows: 1. Mucosubstances of the surface and gastric pit columnar cells were contained a small amount of acid mucosubstances and a large amount of neutral mucosubstances, but the mucous glands were contained only large amounts of neutral mucosubstances and granular cells of gastric glands were contained only a small amount of acid mucosubstances. 2. Acid mucosubstances of the surface and gastric pit columnar cells were contained only a small amount of nonsulfated mucins, but granular cells of gastric glands were contained a small amount of sulfated and nonsulfated mucins.

흰쥐의 설선내 점액질에 미치는 유기인제계 농약의 영향

조운복 부산의대 1977 부산의대잡지 - 부산대학교 의과대학 Vol.17 No.2

경골어 장관 점액세포내 점액질의 조직화학적 성상

조운복,김봉선,최인장,백선용,신인성 부산대학교 1984 자연과학논문집 Vol.37 No.-

In order to investigate the histochemical properties on the mucous cells of proximal, middle and distal intestines in five teleostean species. i. e., Clupanodon Punctatus, Parasilurus asotus, Carassius auratus, Cantherines moderstus, Pagrosomus major, we stained the intestinal mucosae with alcian blue (pH 2.5, pH 1.0 and pH 0.4), periodic acid Schiff (PAS), high iron diamine (HID), alcian blue (pH 2.5) with previous methylation (37℃ and 60℃) and methylation-saponification (60℃), alcian blue (pH 2.5 and pH 1.0)-PAS, aldehyde fuchsin pH 1.7 -alcian blue pH 2.5 and HID- alcian blue pH 2.5 methods. The results obtained were as follows; 1. The mucous cells in the intestinal mucosae of Pagrosomus major, Parasilurus asotus and Clupanodon punctatus were larger in size than those of Cantherines moderstus and Clupanodon punctatus. In Pagrosomus major and Carassius auratus, the mucous cells were distributed all over the intestinal mucosae evenly. In Cantherines moderstus and Clupanodon punctatus, those were distributed mostly in the base and middle portions of the intestinal mucosal folds, but wer found only a few in the top of the intestinal mucosal folds. In Parasilurus asotus, those were distributed all over the intestinal mucosae in large number. 2. The mucous cells of the proximal and middle intestine in Clupanodon punctatus and Pagrosomus major were composed of a few mucous cells having only neutral mucins and most mucous cells with acid and neutral mucins which contains acid mucins more prominent in most cases, but mucous cells with more prominent of neutral mucins were also existed in a few number. The mucous cells of the distal intestine of Clupanodon punctatus, Cantherines moderstus and Pagrosomus major, and those of proximal, middle and distal intestine of Carassius auratus were composed of the neutral and acid mucins, the majority of mucous cells with more prominent of acid mucins and a minority of the mucous cells with more prominent of neutral mucins. The mucous cells of the proximal, middle and distal intestine of Parasilurus asotus were coexisted with the neutral and acid mucins, the majority of the mucous cells had equal content of the acid and neutral mucins and a few mucous cells with more prominent of neutral mucins. The mucous cells of the proximal and middle intestine of Cantherines moderstus were composed of the majority of the mucous cells which were having only neutral mucins or having neutral and acid mucins coexistance with more prominent of neutral ones and the minority of those had neutral and acid mucins with equal content. 3. According to the properties of the acid mucins, the mucous cells of the proximal, middle and distal intestine in Clupanodon punctatus were composed of most mucous cells which contain weak sulfomucins and a little amounts of the carboxylic mucins or sialomucins and a few mucous cells having only small amounts of sialomucins. The mucous cells of the proximal, middle and distal intestine of Carassius auratus were composed of most mucous cells which contain weak sulfomucins and a little amounts of carboxylic mucin or sialomucins and the moderate amounts of strong sulfomucin and a few mucous cells having only small amounts of sialomucin. The mucous cells of the intestine of Parasilurus asotus were composed of most mucous cells which contain only the moderate amounts of strong sulfomucin, and a few mucous cells having weak sulfomucins and sialomucin. The mucous cells of the proximal intestine of cantherines moderstus were composed of most mucous cells which contain weak sulfomucins and a small amounts of carboxylic mucins or sialomucins and, a few mucous cells having only moderate amounts of strong sulfomucins or sialomucins. The mucous cells of the middle intestine of Cantherines moderstus were composed of most mucous cells which contain weak sulfomucins and sialomucins, and a few mucous cells having only small amounts of strong sulfomucins. The mucous cells of the distal intestine of Cantherines moderstus were composed of most mucous cells which contain only large amounts of strong sulfomucins, and a few mucous cells having weak sulfomucins and sialomucins. The mucous cells of the proximal, middle and distal intestine of Pagrosomus major were composed of most mucous cells which contain weak sulfomucins and small amounts of carboxylic mucins, and a few mucous cells having only sialomucins.

참개구리〔Rana nigromaculata(Hallowell)〕설 점액질의 조직화학적 성상

조운복 부산대학교 사범대학 1986 교사교육연구 Vol.13 No.-

This study was performed to clarify the distribution pattern and histochemical properties of lingual mucosubstances in Rana nigromaculata(Hallowell). Mucosubstances were stained with periodic acid Schiff(PAS) reaction, alcian blue pH 2.5, alcian blue pH 0.4, alcian blue pH 2.5-PAS and aldehyde fuchsin pH 1.7-alcian blue pH 2.5 methods. Three general types of epithelial mucosubstances can be identified by the above histochemical techniques; in broad terms they are recognized as sulfomucins, sialomucins and neutral mucins. Surface epithelial cells of lingual dorsum contained moderate amounts of neutral mucins only. Most surface cells of filiform papillae also contained moderate amounts of neutral mucins only while a few surface cells of these papillae appeared to contain moderate amounts of neutral mucins but in addition contain minimal amounts of sialomucins. Surface epithelial cells in basal portion of fungiform papillae were presented only moderated amounts of neutral mucins, but the number of these cells in upper portion of the above papillae occurred abundantly which have mixture of moderate amounts of neutral mucins and sialomucins, and a small number of these cells also contained moderate amounts of neutral mucins and sialomucins but in addition contained minimal strongly acidic sulfomucins. Mucous cells in the apex and inferior surface of tongue contained moderate amounts of neutral mucins and minimal amounts of sialomucins, but lingual glands, on the other hand, contained moderate amounts of neutral mucins only.

제초제 Gramoxone이 흰쥐의 장관점막내 Alkaline 및 Acid Phosphatase 활성에 미치는 영향에 관한 조직화학적 연구

조운복 부산대학교 1982 자연과학논문집 Vol.33 No.-

This experiment was performed to observe the effect of the pyridine herbicide, Gramoxone, on the activities of alkaline and acid phosphatases in the mucosa of the duodenum, jejunum, ileum, proximal colon, distal colon and rectum. Healthy adult rats weighing about 250~300gm each were administered Gramoxone, orally, and sacrificed in 1,3,6,12,24,48 and 72 hours after Gramoxone administration. A change of the alkaline and acid phosphatase activity in the intestinal mucosa were studied with modified Gomori's methods. The obtained results were as follow : 1. Activity of alkaline phosphatase in the small intestinal mucosa appeared to be suppressed from 1 hour after Gramoxone administration. Decrease of the enzyme activity was most striking in the duodenum and jejunum 1,3 and 12 hours, in the ileum 3,6 and 12 hours after Gramoxone administration. Decreased alkaline phosphatase activity showed the tendency to recover in the ileum 24 hours, in the duodenum and jejunum 48 hours after Gramoxone administration. 2. Activity of alkaline phosphatase in the large intestinal mucosa also appeared the tendency to inhibit from 1 hour after Gramoxone administration. Marked decrease of the enzyme activity was noted in the proximal colon 1, 3, 6 and 12 hours, in the distal colon and rectum 3 hours, and appeared to recover in the distal colon and rectum 12 hours, in the proximal colon 48 hours after Gramoxone administration. 3. Activity of acid phosphatase in the columnar cells of the small intestinal mucosa generally showed the tendency to increase in the Gramoxone treated rats. Most marked increase of this enzyme activity was noted in the jejunum 6 hours, in the duodenum 6 and 12 hours, and remarkable increase of this enzyme activity was observed in the jejunum and ileum 3, 12 and 24 hours, in the duodenum 3, 24 and 48 hours after Gramoxone administration. Increased acid phosphatase activity showed the tendency to recover in the ileum 24 hours, in the jejunum 48 hours, and in the duodenum 72 hours after Gramoxone administration but did not appear markedly. 4. Activity of acid phosphatase in the columnar cells of the large intestinal mucosa showed slightly enhanced in the colon 12 and 24 hours, in the rectum 24 hours, and appeared to recover in the colon and rectum 48 hours after Gramoxone administration.

경남지역 고등학교 과학교사의 환경교육에 대한 의식조사 : Centered on the Kyungnam area

조운복,허홍욱,박원혁,최병태 부산대학교 사범대학 과학교육연구소 1994 科學敎育硏究報 Vol.21 No.-

To investigate science teacher's knowledge and attitude toward the environmental education in the senior high school, a questionnaire was administered. 347 science teachers were selected from public and private senior high schools in Kyungnam area. The results obtained were as follows; 1. Most of science teachers(97.98%) were interested in environmental education, but 28.24% of them were educated regarding environmental education through teacher's discipline(42.86%) and university classes(40.82%). Respondents indicated that the knowledge of environmental education was useful when they took classes. Also, 97.99% of science teachers preferred more classes about environmental education. 59.05% of science teachers felt that the environment problem on the region of their residence is very serious for humans such as water pollution(76.37%), garbage pollution(57.35%), and air pollution(55.33%). About science teacher's knowledge regarding environmental education, they were aware of only 12.69% in 30 questions. 2. The results indicated that most of science teachers(99.42%) expected the necessity of systematic environmental education in junior high school curriculum. The respondents indicated that all education system(69.45%); all teachers take charge of environmental education(49.85%), and 92.51% of the science teachers expected appropriate modification of contents regarding environmental education in current science book. As, 43.80% of the science teachers mentioned the environmental education should be taught as a separated subject in schools. As, 95,68% of the science teachers indicated that environmental education needs also to be implemented into the university curriculum. 3. The results of this study indicated that science teachers want to emphasize air pollution(4.36 points), water pollution(4.35 points), soil pollution(4.16 points), and environment integrity(4.12 points) in the high school curriculum. Respondents want to emphasize water pollution(4.39 points), air pollution(4.38 points), soil pollution(4.20 points), and environment integrity(4.05 points) in the senior high school curriculum. Also respondents felt that documentary(4.42 points), guide books(3.93 .points), and movie(3.93 points) were important for environmental education reference. 4. The results of this study indicated that 77.23% of science teachers have teaching experience about environmental education. According to the results, 2.88% of science teachers believed that students are well informed regarding environmental education subjects. 84.15% of science teachers believed that entrance examinations for college affect environmental education. Also, 48.99% of science teachers only taught environmental education in related classes. Most of respondents appeared not to teach environmental education due to fact they do not have time and reference. By way of solution to difficult problems, newspaper and journal(36.89%), and technical environmental education books(32.28%) were helpful. Respondents want the support of related journals, books, and references(40.63%). Also, almost science teachers(97.41%) believed that current contents of environmental education references are not enough to modify the attitude and the behavior of student. 5. Almost, half of science teachers did not know about environmental education policy of Korea(35.73%) and other countries(44.67%), and environment policy(38.62%). But 70.89% of science teachers tried to teach students to modify the attitudes and the behaviors of student regarding environmental education, Also, 67.72% of science teachers believed their role has a strong effect on students. The problems about the environment issue in Korea are the people's awareness(4.37 points), immoral business practices(4.36 points), and environment policy(4.15 points). 6. Judging from above results, although science teachers have a deep interesting in environmental education, environmental education was not enough for changing the student's attitude due to the lack of time and materials. Furthermore, science teachers themselves were in short of knowledge and experience about environmental education, so they should be educated through a systematic teacher's discipline.