복숭아 품종별 성숙정도에 따른 항산화 및 항염증 효과간의 상관관계

정경미,김산영,이상한,Jung, Kyung-Mi,Kim, San Yeong,Lee, Sang-Han 한국식품저장유통학회 2017 한국식품저장유통학회지 Vol.24 No.5

본 연구에서는 복숭아의 품종인 미황, 가납암백도 및 천홍을 성숙정도에 따라 미숙과와 성숙과로 분류하여 항산화 및 항염증 효과간의 상관관계 등을 분석함으로서 향후 염증 및 산화스트레스와 관련된 질병의 예방 또는 치료할 수 있는 기능성 소재를 발굴하고자 수행하였다. 총 페놀 및 플라보노이드 함량은 3품종 모두 성숙과에 비해 미숙과에서 함량이 많았고, 미숙과 중에서는 천홍이, 성숙과 중에서는 미황이 유의성 있게 높은 함량을 나타내었다. 항산화 활성은 미숙과 천홍에서 가장 높았고, 성숙과에서는 DPPH 라디칼 소거능 측정에 의해서는 미황이 유의성 있게 높았으나 FRAP과 CUPRAC 측정에서는 3품종간 유의적인 차이가 없었다. RAW 264.7 세포에서는 미황 미숙과 추출물이 약 30%의 NO 생성 억제 효과가 관찰되었고, 가납암백도 미숙과는 약 29%, 천홍 미숙과는 약 20%의 저해활성을 보이지만, 복숭아 성숙과 3품종에서는 NO 생성 억제 효과가 나타나지 않았다. 3품종 모두 성숙과에 비해 미숙과에서 저해 활성이 높게 나타났다. 총 페놀 함량과 플라보노이드 함량에 따른 항산화 활성들 간의 상관관계에서 고도의 정의 상관성을 나타내었으며, 항염증 활성과 항산화 활성, 항염증 활성과 총 페놀 및 플라보노이드 함량은 낮은 상관관계를 나타내었다. This study was performed to investigate the correlation between anti-oxidant and anti-inflammatory activities in ripe and unripe fruits of three peach cultivars: Miwhang (MH), Kanoiwa hakuto (KH) and Cheonhong (CH). The unripe fruits had higher levels of total phenols and flavonoids contents than those in the ripe fruits of all the three cultivars. The unripe fruits of CH showed the highest levels of total phenols, flavonoids and antioxidant activities among the fruit samples analyzed. Nitric oxide inhibition values in RAW 264.7 cells for the unripe fruits of MH and KH were 30 and 29%, respectively. However, the inhibition was not observed in unripe CH and the ripe fruits of either cultivar. Total phenols and flavonoids contents showed high linear correlations with the anti-oxidant activities whereas the anti-inflammatory activity had low linear correlations with them.

복숭아 분말 첨가 초콜릿과 초콜릿 코팅 복숭아 스낵의 제조 및 품질 특성

정경미(Kyung-Mi Jung),박성기(Sung-Gi Park),이영석(Youngsuk Lee),김수린(Soo Rin Kim) 동아시아식생활학회 2017 동아시아식생활학회지 Vol.27 No.5

농지임대차법제의 개선방안에 관한 연구

정경미 ( Kyung Mi Jung ) 제주대학교 법과정책연구소 2013 국제법무 Vol.5 No.1

최근 귀농·귀촌 등의 원인으로 농지의 사용·수익이 늘어나고 이로 인해 농지임대차가 급증하고 있는 추세이다. 임대차를 통한 농지의 이용에 있어서 농지임대차에 관한 규정의 미비점으로 인해 임차인의 지위가 불안하고 이로 인한 사회적인 문제까지 발생하고 있다. 농지법은 ``경자유전의 원칙`` 아래 농지에 대한 임대차를 원칙적으로 금지하고 있다. 농지임차인을 보호하기 위하여 주택임차인의 보호수준에서 농지임차인을 강력하게 보호하여 왔으나, 농업경쟁력의 저하로 인하여 그 보호의 필요성이 결여되어 농지임차의 보호에 관한 여러 조문들을 폐지하고 서면계약의 원칙, 임대차 기간, 임대차계약에 관한 조정, 묵시의 갱신, 임대인의 지위승계 등 몇 가지 규정만을 두고 있다. 반면에 일본, 독일, 기타 유럽에서는 농지임대차법제를 마련하여 임차인 보호를 하고 있다. 그러나 우리나라에서 농지임대차는 임차인의 보호에 미흡하고, 이에 각국의 농지임대차에 관한 입법례를 살펴봄으로써 다양한 개선방안을 찾아보고자 한다. 농지임대차는 앞으로도 지속적으로 증가할 것으로 예상되고 있는데 이에 농지문제를 "농업생산성 제고와 농지의 합리적 이용"으로 인식한다면 농지임대차에 있어서도 단순히 허용·금지의 차원을 떠나 농업생산성을 제고하고 어떻게 합리적으로 농지를 이용할 수 있을 것인가의 관점에서 농지임대차에 관한 규정의 미비점을 보완해 나아가야 할 것으로 판단된다. Recently, the number of people becoming farmers has increased. As a result, more farmlands have been used and more profits have been created, while the level of farmland lease has increased. Regarding the use of leased farmlands, there seem to be weak points regarding the regulations about the lease of farmlands, causing the lessee`s position unstable and generating various social problems. The farmland act prohibits the lease of farmlands in principle under the ``Principle of Farmland Ownership``. The lessees of farmlands have been strongly protected as housing lessees. However, due to the reduction of the agricultural competitiveness, such a kind of protection has become worsened, causing various articles for the protection of the lessees of farmlands to be abolished. As a result, there seem to be only a few regulations left, including the principles of written contracts, the period of lease, the adjustment of the lease contracts, the renewal of implied, and the succession of lessor`s status. Meanwhile, such countries as Japan, Germany and other European countries have provided various farmland lease laws in order to protect lessees. However, in Korea, there seem to be not enough laws to protect the lessees of farmlands. Therefore, this study focuses on various methods to improve such laws in Korea by observing the legislation cases related to the lease of farmlands in each countries. It is expected that the level of farmland lease will continue to increase. If the problems related to farmlands are recognized in terms of "the consideration of agricultural productivity and the reasonable use of farmlands", it will be possible to consider the agricultural productivity beyond the aspects of allowance` prohibition for the lease of farmlands. Also, it will be possible to supplement the weak points of the regulations related to the lease of farmlands by figuring out how reasonable farmlands can be used.

존 던의 연애시: 역설의 미학

정경미 ( Kyung Mi Jung ) 한국현대영어영문학회 2012 현대영어영문학 Vol.56 No.2

The purpose of this paper is to reveal John Donne`s paradoxical and reversal strategy in his early love poems. Donne`s readers were mainly the witty and sophisticated readers, Inns gentlemen. He shared with the coterie readers to whose capacities for dramatic involvement he appealed. He created a new style of the lyric poems. The best way to send his concealed concern was to express it not directly and honestly but reversely and paradoxically. Most of the poems he wrote while studying at Lincoln`s Inn are cynical and epigrammatic toward woman`s infidelity and inconsistency. The epigrammatic is fused with other elements, depending on the rhetorical and paradoxical coordinates of the lyrics. The witty poets and the witty readers were supposed to be capable of sustaining both an imaginative involvement in and a critical attitude toward poems―that is, to write and read both poetically and paradoxically. The reversal elements of his poems gave him the reputation of being a supremely witty writer. Whether libertine or courtly, argumentative or dramatic, Donne` lyrics characteristically blend their forms and modes to create the kinds of poems he and his readers liked. (Hanbat National University)

Balb/c 마우스를 이용한 비방사선 BrdU:ELISA 국소림프절 시험법의 연구

정경미 ( Kyoung Mi Jung ),장원희 ( Won Hee Jang ),박영호 ( Young Ho Park ),임경민 ( Kyung Min Lim ) 한국동물실험대체법학회 2010 동물실험대체법학회지 Vol.4 No.1

To avoid the use of radioisotopes, many efforts have been made to develop non-radioisotopic modifications of the LLNA. Recently, OECD presented a guideline for the nonradioisotopic murine local lymph node assay employing BrdU incorporation and ELISA. This method was a non-radioisotopic modification of the LLNA using 5-bromo-2′-deoxyuridine (BrdU) incorporation. However, this method recommends CBA strain mice which is not common in Korea. Here we evaluated the sensitivity of the LLNA:BrdU-ELISA using the Balb/c mice strain, which is a readily available strain in Korea. According to OECD draft guideline, female Balb/c mice were treated for 3 days with a test material or vehicle to each side of both ears and BrdU was given as a intraperitoneal administration at 5 mg/mouse. We found that Balb/c mice is sensitive to BrdU and some premature deaths were found in the treatment groups. Balb/c mice must be older than 11 weeks to tolerate BrdU. BrdU incorporation into draining lymph node cells was measured using ELISA. In this study, isoeugenol, hexylcinnamic aldehyde(HCA), eugenol and isopropanol(IS) were used as reference chemicals. When the 1.6 or greater SI values were defined as sensitizer according to OECD draft guideline, isoeugenol, HCA and eugenol which are classified as moderate and weak sensitizers in the traditional LLNA, were found to evaluate positive results. LLNA:BrdU-ELISA using Balb/c mice, sensitivity and specificity were 100%. The EC1.6 values for isoeugenol, HCA and eugenol were calculated to be 1.8, 19.3 and 15.5% in 5 mg BrdU/mouse. A positive correlation (R2=0.813) between traditional LLNA and LLNA:BrdU-ELISA strongly suggests that the LLNA:BrdU- ELISA could be used as an alternative method to screen allergen.

저온 알코올 발효를 통한 고품질 복숭아 식초의 생산

정경미(Kyung-Mi Jung),이영석(Youngsuk Lee),김정원(Jeong-won Kim),설정만(Jeongman Seol),정영훈(Young Hoon Jung),김수린(Soo Rin Kim) 한국생물공학회 2018 KSBB Journal Vol.33 No.2

As health benefits of traditional vinegar is newly recognized, vinegar products are being developed with new and enhanced functionalities to meet customers’ diverse demand. Meanwhile, peach is one of the crops with largest production in Korea but neither its preservation nor its processing is simply done. For the development of a new vinegar product using peach, the present study focused on improving an alcoholic fermentation process using a new cold-tolerant Saccharomyces cerevisiae strain isolated from peach. Specifically, alcoholic fermentation of peach concentrates was performed at a control (30℃) or a low (10℃) temperature, followed by conventional acetic acid fermentation at 25℃, to investigate the effect of low-temperature alcoholic fermentation (LTAF) on the final vinegar quality. We measured free amino acid contents and flavor compound profiles, and performed sensory evaluation of the vinegar products. As a result, the LTAF vinegar had higher free amino acid contents and lower off-flavor intensity than those in the control vinegar. Moreover, overall preference of the LTAF vinegar was significantly higher than the control vinegar. In conclusion, the present study demonstrated that a promising potential of using the LTAF process for the development of high quality vinegar products.

연구논문 : Balb/c 마우스를 이용한 비방사선 BrdU:ELISA 국소림프절 시험법의 연구

정경미 ( Kyoung Mi Jung ),장원희 ( Won Hee Jang ),박영호 ( Young Ho Park ),임경민 ( Kyung Min Lim ) 한국동물실험대체법학회 2010 동물실험대체법학회지 Vol.4 No.1

To avoid the use of radioisotopes, many efforts have been made to develop non-radioisotopic modifications of the LLNA. Recently, OECD presented a guideline for the nonradioisotopic murine local lymph node assay employing BrdU incorporation and ELISA. This method was a non-radioisotopic modification of the LLNA using 5-bromo-2′-deoxyuridine (BrdU) incorporation. However, this method recommends CBA strain mice which is not common in Korea. Here we evaluated the sensitivity of the LLNA:BrdU-ELISA using the Balb/c mice strain, which is a readily available strain in Korea. According to OECD draft guideline, female Balb/c mice were treated for 3 days with a test material or vehicle to each side of both ears and BrdU was given as a intraperitoneal administration at 5 mg/mouse. We found that Balb/c mice is sensitive to BrdU and some premature deaths were found in the treatment groups. Balb/c mice must be older than 11 weeks to tolerate BrdU. BrdU incorporation into draining lymph node cells was measured using ELISA. In this study, isoeugenol, hexylcinnamic aldehyde(HCA), eugenol and isopropanol(IS) were used as reference chemicals. When the 1.6 or greater SI values were defined as sensitizer according to OECD draft guideline, isoeugenol, HCA and eugenol which are classified as moderate and weak sensitizers in the traditional LLNA, were found to evaluate positive results. LLNA:BrdU-ELISA using Balb/c mice, sensitivity and specificity were 100%. The EC1.6 values for isoeugenol, HCA and eugenol were calculated to be 1.8, 19.3 and 15.5% in 5 mg BrdU/mouse. A positive correlation (R2=0.813) between traditional LLNA and LLNA:BrdU-ELISA strongly suggests that the LLNA:BrdU- ELISA could be used as an alternative method to screen allergen.

남성의 성역할 갈등이 도움추구 태도에 미치는 영향: 수치심과 자기공개기대의 매개효과

정경미 ( Kyung Mi Jung ),신희천 ( Hee Cheon Shin ) 서강대학교 학생생활상담연구소 2014 人間理解 Vol.35 No.2

본 연구는 남성의 성역할 갈등과 상담에 대한 도움추구 태도의 관계에서 수치심, 자기공개 기대(위험성, 유용성)의 매개효과를 알아보기 위해 매개 모형을 검증하였다. 233명의 남성들을 대상으로 실시하였으며, 전체 대상 중 200명이 남자 대학생으로 표집 되었다. 자료 분석은 성역할 갈등, 수치심, 자기공개기대(위험성, 유용성), 도움추구 태도 간의 관계를 알아보기 위해 상관분석을 하였으며, 변인들 간의 구조를 알아보기 위해 구조방정식을 적용하여 분석하였다. 이에 대한 연구 결과는 다음과 같다. 성역할 갈등은 수치심, 자기공개기대 위험성과는 정적인 관계를, 상담에 대한 도움추구태도, 자기공개기대 유용성과는 부적인 관계로 나타났다. 또한 성역할 갈등과 상담에 대한 도움 추구 태도의 관계에서 수치심 그리고 자기공개 기대 위험성, 자기공개기대 유용성은 각각 매개변인으로 작용하였다. 즉, 수치심, 자기공개 기대 유용성과 위험성은 성역할 갈등과 상담에 대한 도움 추구 태도에 영향을 주는 것을 확인할 수 있었다. 끝으로 본 연구의 의의 및 제한점과 향후 대학상담실제에 적용할 수 있는 방향을 기술하였다. This study examined the mediating role of shame and self-disclosure expectation (risk, utility) on the relationship between men’s gender role conflict and attitudes toward counseling. A total of 233 male adults participated in the survey. Correlation coe?cients were examined data collected related to gender role con?ict, shame, self-disclosure expectation (risk, utility) and professional help seeking attitudes. The results revealed that gender role conflict was positively related to professional psychological help seeking behavior, shame and anticipated risks; however, gender role con?ict was negatively related to anticipated utilities. Result also indicated that shame and self-disclosure expectation mediated the relationship between gender role con?ict and help seeking attitudes.

복숭아 ‘천중도백도’ 이산화염소 처리에 의한 부패 억제효과 및 숙도별 호흡패턴

정경미(Kyung-Mi Jung),이숙희(Suk-Hee Lee),김임수(Im-Soo Kim),김산영(San-Yeong Kim),김대홍(Dae-Hong Kim) 한국원예학회 2012 한국원예학회 학술발표요지 Vol.2012 No.10

AP 콜라겐 효소분해 펩타이드 섭취가 피부 주름, 탄력 및 보습 개선에 미치는 효과

정경미(Kyoungmi Jung),양미숙(Mi-Suk Yang),김승훈(Seung Hun Kim),노종화(Jonghwa Roh),김완기(Wangi Kim) 한국식품영양과학회 2021 한국식품영양과학회지 Vol.50 No.5

본 연구에서는 40~60세 성인 여성을 대상으로 APCP의 피부 주름, 탄력 및 보습 개선에 대한 이중눈가림, 무작위배정, 대조식품 비교 인체적용시험을 진행하였다. 눈가 주름 부위의 육안평가 및 Visiometer를 이용한 피부 주름 개선 평가에서 APCP 1,000 mg 및 1,500 mg 섭취군 모두 12주에 대조식품 섭취군 대비 유의적인 개선 효과를 보였고, 섭취종료 후 2주까지 피부 주름 개선이 유지됨을 확인할 수 있었다. 피부 탄력 개선 평가에서 R2, R5 및 R7 지표에 대해 APCP 1,000 mg 및 1,500 mg 섭취군 모두 6주부터 대조식품 섭취군 대비 유의적인 개선 효과를 보였고 12주까지 통계적으로 유의한 차이를 나타내었다. 피부 보습 개선 평가에서도 APCP 1,000 mg 및 1,500 mg 섭취군 모두 12주에 대조식품 섭취군 대비 유의적인 개선 효과를 보였고 섭취종료 후 2주까지 피부 보습 개선 상태가 유지되었다. 연구를 진행하는 동안 이상반응, 임상실험실 검사 및 활력징후 검사에서 유의미한 변화는 나타나지 않아 APCP의 안전성을 확인할 수 있었다. 본 인체적용시험 결과를 통해 APCP는 피부주름, 거칠기, 탄력 및 보습과 같은 피부 건강 개선에 도움을 줄 수 있는 건강기능식품임을 확인하였다. Collagen is a component of the human body, known to have a beneficial effect on human health when consumed as a food or dietary ingredient. This double-blinded, randomized, placebo-controlled study aimed to evaluate the efficacy and safety of AP collagen peptides (APCP) containing 3% glycine-proline-hydroxyproline, on improving skin wrinkles, elasticity and hydration. Totally, 105 subjects with crow’s-feet wrinkles were randomly assigned to receive either placebo, APCP 1,000 mg or APCP 1,500 mg, once daily for 12 weeks. Efficacy endpoints of skin wrinkles, elasticity and hydration were measured at baseline, 6 and 12 weeks, and at the 2-week follow-up visit. Compared to the placebo group, the visual evaluations and R3 (average roughness) values of crow’s-feet wrinkles were significantly decreased in both APCP groups at 12 weeks, and these effects were maintained until 2 weeks after completion of ingestion. During the intake period at 6 and 12 weeks, three parameters for skin elasticity (R2, R5, R7) showed significant improvement in both APCP groups, as compared to the placebo group. The APCP groups also showed a statistically significant difference in skin hydration compared to the placebo group, at 12 weeks and at 2 weeks after the end of intake. No adverse effects were encountered during the study period. These results indicate that APCP 1,000 mg and 1,500 mg can be used as a functional food ingredient to improve human skin conditions related to skin aging and hydration.