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심한 양수과다증을 동반한 무심장 쌍둥이 임신에서 복부내 제대동맥의 알코올 절제술 (alcohol ablation) 및 양수감압술의 성공적인 자궁내 치료
이시원 ( Si Won Lee ),이선옥 ( Sun Ok Lee ),박미혜 ( Mi Hye Park ),김영주 ( Young Ju Kim ),전선희 ( Sun Hee Chun ),안정자 ( Jung Ja Ahn ),김종일 ( Chong Il Kim ),조민선 ( Min Sun Cho ) 대한산부인과학회 2004 Obstetrics & Gynecology Science Vol.47 No.7
감자T바이러스 검정을 위한 RT-PCR 및 Nested PCR 진단시스템 개발
이시원(Si Won Lee),신용길(Yong-Gil Shin),이진영(Jin-Young Lee),김영석(Young-Suk Kim),양미희(Mi Hee Yang),최인철(In-Cheol Choi) 충남대학교 농업과학연구소 2015 농업과학연구 Vol.42 No.2
Potato virus T (PVT) is a plant pathogen in the family Betaflexiviridae, group IV single-stranded positive sense RNA viruses. The major host of PVT is potato, and it has been reported in Ullucus tuberosus, Oxalis tuberosa and Tropaeolum tuberosum. This study aimed at developing reverse transcription (RT)-polymerase chain reaction (PCR) and nested PCR techniques for specific detection of PVT. Finally, Two RT-PCR primer sets were developed and verified. The RT-PCR products were amplified to 734 (PVT RT-PCR primer set 6) and 828 bp (PVT RT-PCR primer set 29) long to detect PVT. The nested PCR primer sets [PVT-N70/C20 (734→315 bp) and PVT-N75/C30 (828→529 bp)] were developed which are high sensitivity and verification for detection of PVT. Furthermore, a modified-positive control plasmid is use to verify contamination of laboratory in PVT detection. This study supported the diagnose PVT in potato or PVT related hosts.