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이만상,김중래,한창열 한국수산학회 1981 한국수산과학회지 Vol.14 No.1
As one of the fundamental studies for the breeding of marine algae, this paper deals with the effects of plant hormone on the growth of microscopic filamentous gametophyte of undaria pinnatifida. The results obtained are summarized as follows: (1) All zoospores were settled on slide glass, germinated and developed into gametophytes, without the growth of germination tubes when treated with 0.1 mg/ℓ of 2.4-D, or 0.1, 1.0 or 5.0 mg/ℓ of keinetin (2) The best growth effect in total average was observed at 0.1 mg/ℓ of Keinetin, when the growth-rate was 248.9% in contrast with control, and was followed by 1.0 mg/ℓ of IAA (243.3%), and 0.05 mg/ℓ of 2.4-D (205.6%) (3) It was certain that the growth-effect by each plant hormone had some differences between male and female gametophytes. IAA was very effective in the growth of male gametophytes but Keinetin in that of females. Especially in females, the efficiency of Keinetin was recorded best at 239.0% at 5.0 mg/ℓ and 222.0% at 0.1 mg/ℓ. On the other hand, it was 195.1% in its best at 0.5 mg/ℓ of IAA, and 146.6% was recorded best 2.4-D at 0.05 mg/ℓ.
이만상 한국식물생명공학회 1995 식물생명공학회지 Vol.22 No.5
감귤류 속간교잡에 의하여 왜성화되고 내한성이 강한 신품종 개발에 대한 연구로서 황금유자와 탱자, 유자와 탱자의 정역교잡 및 잡종종자의 미숙배주 배양을 하였던 바 그 결과는 다음과 같다. 미숙배주의 callus형성은 탱자 < 유자 < 황금유자 $\times$ 탱자 < 황금유자 순서로 양호했는데, 1 ~ 3 mg/L + zeatin 0.5 mg/L 처리구에서 제일 좋았다. 황금유자와 탱자의 정역속간교잡 20주 후 기내에서의 발아율은 황금유자 $\times$ 탱자의 잡종이 41.3%, 탱자 $\times$ 황금유자의 잡종에서는 37.7%를 보였다. 황금유자 $\times$ 탱자의 F$_1$ 잡종 95%와 탱자 $\times$ 황금유자의 F$_1$ 잡종 100%는 탱자를 닮았다. 황금유자 $\times$ 탱자의 수분 후 3시간이면 화분은 계두상에서 발아하고, 24~28시간이면 화분관이 주공에 진입하며, 2일이면 극핵과 수정하며, 3일이면 난세포와 수정한다. 속간교잡의 결과율은 황금유자 $\times$ 탱자가 14.0%, 탱자 $\times$ 황금유자가 17.5%이었다. 황금유자와 탱자의 인공자가수정 결과율은 각각 45.4%, 27.5%이었으며 인공 타가수정 결과율은 34.2%, 39.5% 이었고, 단위결과율은 3.1%, 1.4%이였다. 또한 자연상 태에서의 결과율은 각각 13.0%, 3.0% 이었다. 황금유자, 유자, 탱자의 체세포와 생식세포 염색체수는 모두 2n=18, n=9 이었다. This study was carried out to develop new varieties which are dwarf and tolerant to winter cold in the Aurantioideae by intergeneric crossing. to do that, the reciprocal crosses of Hwanggeumyooza and trifoliate orange, yooza and trifoliate orange were done and in vitro immature ovule culture of their hybrid was carried out .The callus formation from immature ovule was good in order of Hwanggeumyooza, Hwanggeumyooza $\times$ tifoliate orange, yooza, and trifoliate orange and best at 1 to 3 mg/L NAA+0.5mg/L zeatin on MT medium. In vitro germination percentage of 20week old hybrid of Hwanggeumyooza $\times$ tifoliate orange and trifoliate orange $\times$ Hwanggeumyooza were 41.3% and 37.7, respectively. The phenotype of hybrid (95%) of Hwanggeumyooza $\times$ trifoliate orange and that (100%) of trifoliate orange $\times$ Hwanggeumyooza were similar to that of trifoliate orange. After Hwanggeumyooza was pollinated by pollens of trifoliate orange, the pollen tubes grew on stigma after 3h of pollination and entered into micropyle after about 24~28 h. One gamete in pollen was fused with polar nuclei after 2 days and other one fused with egg nucleus at 3days after pollination. The fruit set percentage by intergeneric crossing was 14.0% in Hwanggeumyooza $\times$ trtfoliate orange and 17.5% in trifoliate orange $\times$ Hwanggeumyooza. The fruit set percentages of Hwanggeumyooza. and trifoliate orange were 34.2% and 39.5% by artificial self-fertilization, 34.2% and 39.5% by artificial cross fertilization, 3.1% and 1.4% by parthenocarpy and 13.0% and 3.0% by natural fertilization, respectively. The somatic and gametic chromosome numbers of Hwanggeumyooza, yooza, and trifoliate orange were 2n=18 and n=9.
해조의 육종을 위한 기초적 연구 3 . 김 엽체에 미치는 식물호르몬의 생장효과
이만상,김중래,한창열,조영원 한국수산학회 1981 한국수산과학회지 Vol.14 No.2
김葉體의 生長에 미치는 植物호르몬의 영향을 調査하기 위해서 Gibberellin, IAA, Kinetin, NAA 및 2.4-D 等을 濃度別로 희석한 培地에서 김葉片을 32日間 培養하고 또 2次的으로 Kinetin의 濃度別培地에서 水溫을 5℃와 10℃로 區分하여 18日間 培養하였다. 그 結果를 要約하면 다음과 같다. (1) Gibberellin은 10.0㎎/ℓ濃度에서, 其他는 모두 5.0㎎/ℓ 濃度의 培地에서 各各 最高生長效果가 나타났다. 乾重量으로 본 效率値는 Kinetin이 312.5%(葉面積 : 345.3%)로 으뜸이며 2.4-D는 287.5%(236.1%), Gibberellin 247.9(241.9)%, IAA, 166.7(147.6)%, NAA 141.7(167.7)%의 順이였다. (2) 特히 Kinetin 5.0㎎/ℓ濃度에서 培養된 葉片은 그 色彩가 진한 黑褐色으로 가장 좋았으며 細胞마다 생기있는 큰 色素體가 뚜렷하고 健全하여 뛰어난 效果가 나타났으나 餘他는 색채가 淡褐色 또는 黃綠褐色으로 변하고 死細胞가 많이 發見되었으며 生細胞도 原形質이 萎縮되었거나 큰 液胞가 생겨 매우 不健全하였다. (3) 低溫(5℃)과 高溫(10℃)에서의 Kinetin의 生長效率은 低濃度에서는 大差가 없었으나, 5.0㎎/ℓ의 高濃度에서는 低溫이 366.7%, 高溫이 318.8%로서 48%의 效率差가 생겼으며 細胞의 健全性은 1次實驗때와 마찬가지로 모두 좋았다. (4) 最高生長效果가 나타난 Kinetin 5.0㎎/ℓ 濃度에서는 葉片의 緣邊에 지름 25.0∼27.5μ 정도의 異常大型細胞가 많이 發見되었으며 그 중에는 한 細胞內에 數個의 包素體가 있는 것이 觀察되었다. 두께도 41.0∼42.0μ으로 比較的 두꺼웠다. (5) Kinetin 1.0 ㎎/ℓ 濃度의 培地에서 培養된 葉片中에는 造精器을 形成하고 있는 세포群이 觀察되었다. As one of the fundamental studies for the breeding of marine algae, the effects of several plant hormones (IAA, Gibberellin, 2.4-D, NAA, Kinetin) on the growth of Porphyra-fronds, P. tenera Kjell. form tamatsuensis Miura, were investigated from January 21 to February 21 1981. The fronds used for the experiment were dissected out at 25㎟ size, and cultured in modified Provasoli's ESP medium supplemented with various concentrations of each plant growth regulators. The culture was kept under constant water temperature of 5℃ in 14 hrs. photoperiod and illuminated with 2,400 lux by fluorescent light. Based on the results of first experiment, the culture of fronds for the secondary experiment was carried out at 5℃ and 10℃ in medium containing various levels of Kinetin from April 6 to 24, and compared the growth of two groups at each concentrations with each other. The results obtained are summarized as follows: (1) The best growth efficiencies were observed at 5.0㎎/ℓ of each plant hormones except Gibberellin. Among them, the highest growth-rate was 312.5%(345.3% in frond size) in contrast with control at 5.0㎎/ℓ of Kinetin, and was followed by 287.5%(236.1%) in 2.4-D, 166.7%(147.6%) in IAA and 141.7%(167.7%) in NAA, but that in Gibberellin was 247.9% (241.9%) at 10.0㎎/ℓ. (2) Especially, the fronds cultured at 5.0㎎/ℓ of Kinetin were deep black-brown in colour, and had vivid, healthy chloroplasts in their all cells. On the contrary, the fronds cultured in other media were discoloured to light black-brown or green-brown, and almost all cells were vacuolated or shrunk gradually into death.