수도종자의 방사선조사에 있어서 열처리의 효과

韓昶烈,元鍾樂,崔光泰 韓國作物學會 1971 Korean journal of crop science Vol.- No.10

방사선조사시 열처리의 효과를 알기 위하여 수도종자에 방사선조사시 열전후처리를 행하였던바M1 세대의 생물학적인 반응 및 M2 세대의 변이율에 대해 몇가지 결과를 얻었다. 1. 방사선선량 20kR에서 열전처리는 방사선단독조사에 비해 생물학적인 장해를 적게하고 변이율을 증가시켰다. 2. 방사선단독조사 및 조사전열처리에 비해 heat shocking으로서 열후처리하는 것은 변이율을 증가시켰다. 3. 열전처리에 의해 M1 세대의 장해가 적고 변이율이증가되는 가장 효과적인 열처리온도 및 시간은 60℃ 에서 30분간이었다. 4. 열처리는 방사선단독조사에 비해 mutation spectrum을 달리했다. For the purpose of finding out the effect of heat treatment on biological response and mutation rate, rice seeds were heat treated before and after gamma irradiation. 1. At a dose of 20 KR, pre-irradiation heat treatment showed reduced biological damage and increased mutation rate as compared with non-heat treatment. 2. Mutation frequency was increased in post-treatment of heat shock than in pre-irradiation heat treatment and non-heat treatment. 3. Pre-irradiation heat treatment at 60~circC for 30 minutes markedly reduced the biological damage and increased the mutation rate. 4. Mutation spectrum in heat treatment was different from non-treatment.

수도 고단백돌연변이계통과 모품종과의 (母品種) 교잡 F3 에 있어서 다발성유전인자의 발현 2

한창열,원종락 한국육종학회 1977 한국육종학회지 Vol.9 No.1

고려 인삼의 육종과 조직배양 ( Breeding of Panax ginseng and Plant Tissue Culture )

한창열 고려인삼학회 1976 Journal of Ginseng Research Vol.1 No.1

방사선 및 약품처리시의 제요인이 벼의 M1 세대에 미치는 영향

한창열,이영일 한국육종학회 1973 한국육종학회지 Vol.5 No.1

고추염색체의 복합전좌합성에 (複合轉座合成) 관한 연구 1 : 전좌 (轉座) homo 개체의 선발

한창열,원종락 한국육종학회 1975 한국육종학회지 Vol.7 No.2

고등식물에 있어서 염색체 이상과 그 이용

한창열 한국산림과학회 1984 한국임학회 학술발표논문집 Vol.1984 No.-

減數分裂, 最近의 進步(I)

한창열 한국식물생명공학회 1998 식물생명공학회지 Vol.25 No.6

During the 100 years since the initial discovery of meiotic phenomenon many brilliant aspects have been elucidated, but further researches based on light microscopy alone as an experimental tool have been found to have some limits and shortcomings. By the use of electron microscopy and armed with the advanced knowledges on modern genetics and biochemistry it has been possible to applu molecular technology in gaining information on the detailed aspects of meiosis. As synapsis takes place, a three-layered proteinous structure called the synatonemal complex starts to form in the space between the homologous chromosomes. To be more precise, it begins to form along the paired chromosomes early in the prophase I of meiotic division. The mechanism that leads to precise point-by-point pairing between homologous chromocomes division. The mechamism that leads to precise point-by-point pairing between homologous chromosomes remains to be ascertained. Several items of information, however, suggest that chromsome alignment leading to synapsis may be mediated somehow by the nuclear membrane. Pachytene bivalents in eukaryotes are firmly attached to the inner niclear membrane at both termini. This attached begins with unpaired leptotene chromosomes that already have developed a lateral element. Once attached, the loptotene chromosomes begin to synapse. A number of different models have been proposed to account for genetic recombination via exchange between DNA strands following their breakage and subsequent reunion in new arrangement. One of the models accounting for molecular recombination leading to chromatid exchange and chiasma formation was first proposed in 1964 by Holliday, and 30 years later still a modified version of his model is favored. Nicks are made by endomuclease at corresponding sites on one strant of each DNA duplex in nonsister chromatid of a bivalent during prophase 1 of meiosis. The nicked strands loop-out and two strands reassociate into an exchanged arrangement, which is sealed by ligase. The remaining intact strand of each duplex is nicked at a site opposite the cross-over, and the exposed ends are digested by exonuclease action. Considerable progress has been made in recent years in the effort to define the molecular and organization features of the centromere region in the yeast chromosome. Centromere core region of the DNA duplex is flanked by 15 densely packed nucleosomes on ons side and by 3 packed nucleosomes on the other side, that is, 2000 bp on one side and 400 400 bp in the other side. All the telomeres of a given species share a common DNA sequence. Two ends of each chromosome are virtually identical. At the end of each chromosome there exist two kinds of DNA sequence" simple telpmeric sequences and telpmere-associated sequencies. Various studies of telomere replication, function, and behabior are now in progress, all greatly aided by molecular methods. During nuclear division in mitosis as well as in meiosis, the nucleili disappear by the time of metaphase and reappear during nuclear reorganizations in telophase. When telophase begins, small nucleoli form at the NOR of each nucleolar-organizing chromosome, enlarge, and fuse to form one or more large nucleoli. Nucleolus is a special structure attached top a specific nucleolar-organizing region located at a specific site of a particular chromosome. The nucleolus is a vertical factory for the synthesis of rRNAs and the assenbly of ribosome subunit precursors.sors.

2n性 單爲생殖 이용에 의한 固定 $F_1$種子 생산과 그 展望

한창열,한지학 한국식물생명공학회 1997 식물생명공학회지 Vol.24 No.4

Plants belonging to the category of 2n apomixis or agamospermy form embryos and seeds without the processes of normal meiosis and syngamy. Seeds produced in this way have identical genotype of their maternal parent. Three different types of agamospermy are recognized: diplospory, apospory, and adventitious (adventive) embryony. $F_1$ hybrid cultivars cannot be used as seed sources in the next ($F_2$) generation because this generation would be extremely variable as a result of genetic segregation. Hybrid vigor is also reduced in the $F_2$ generation. Therefore, parental stocks for hybrid seed production need to be maintained and cross must be continuously repeated. Agamospermic 2n apomixis would make it possible to fix the genotype of a superior variety so that clonal seeds faithfully representing that genotype could be continuously and cheaply produced independent of pollination. That is, $F_1$ hybrid seeds could be produced for many generations without loss of vigor or genotype alteration. Production of apomictic $F_1$ hybrid seed would be simplified because line isolation would not be necessary to produce seed or to maintain parental lines, and the use of male-sterile lines could be avoided. Overall, apomixis would enable a significant reduction in hybrid seed production costs. Additionally, the production of clonal seed is not only important for seed propagated crops, but also for the propagation of heterozygous fruit trees and timbers. Clonal seed would help avoid costly and time-consuming vegetative propagating methods that are currently used to ensure the large-scale production of these plants. Apomixis is scattered throughout the plant kingdom, but few important agricultural crops possess this trait Therefore, most research to date has centered on introgressing the trait of apomixis into agricultural crops such as wheat, maize, and some forage grasses from wild distant relatives by traditional cross breeding. The classical breeding approach, however is slow and often impeded by many breeding barriers. These problems could be surmounted by taking mutagenesis or molecular approach. Arabidopsis thaliana is a tiny sexually reproducing plant and is convenient in constructing and screening in molecular researches. Male-sterile mutants of Arabidopsis are particularly suitable genetic background for mutagenesis and screening for apomictic mutants. Molecular approaches towards isolating the genes controlling the apomictic process are feasible. Direct isolation of genes conferring apomixis development would greatly facilitate the transfer of this trait to wide variety of crops. Such studies are now in progress.

유전자의 체제 및 유전자 발현의 조절

한창열 한국육종학회 1992 한국육종학회지 Vol.24 No.2

Programme 되고, 환경(環境)에 반응(反應)해서 변(變)하는 dynamic 한 genome

한창열 한국육종학회 1988 한국육종학회 학술발표회 발표요지 Vol.20 No.1