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Antioxidant Activities and Total Phenolic Contents of Three Legumes
이경준(Kyung Jun Lee),김가희(Ga-Hee Kim),이기안(Gi-An Lee),이정로(Jung-Ro Lee),조규택(Gyu-Taek Cho),마경호(Kyung-Ho Ma),이수경(Sookyeong Lee) 한국자원식물학회 2021 한국자원식물학회지 Vol.34 No.6
Legumes have been important components of the human diet. They contain not only protein, starch, and dietary fiber, but also various phenolic compounds such as flavonoids and phenolic acids. The importance of phenolic compounds to human health is well known due to their antioxidant activities. In this study, three legumes (adzuki beans, common beans, and black soybeans) frequently cultivated in Korea were evaluated for their total phenolic content (TPC) and antioxidant activities using DPPH (2,2-diphenyl-1-picrylhydrazyl), ABTS (2,2′-azinobis (3-ethylbenzothiazoline 6-sulfonate)), and FRAP (ferric reducing antioxidant potential) assays. In addition, correlations between agricultural traits and antioxidant activities of these three legumes were analyzed. Antioxidant activities assessed by DPPH, ABTS, and FRAP assays and TPC showed wide variations among legumes types and accessions. Among the three legumes, adzuki beans showed higher TPC and antioxidant activity than the other two legumes. In correlation analysis, seed size showed negative correlations with antioxidant activities and TPC. In principal component analysis and hierarchical clustering analysis, each of the three legumes was clearly separate. Results of this study can be used as basic information for developing functional materials for each legume. They can also help us understand the overall antioxidant activity of the three legumes.
이정로 ( Jung Ro Lee ),백형진 ( Hyung Jin Baek ),최유미 ( Yu Mi Choi ),이석영 ( Sok Young Lee ),이기안 ( Gi An Lee ),정연주 ( Yeon Ju Jung ),김정곤 ( Chung Kon Kim ),이명철 ( Myung Chul Lee ) 한국국제농업개발학회 2011 韓國國際農業開發學會誌 Vol.23 No.5
China is a one of the largest agricultural countries in the world. China consumes around 12.5 billion kilograms of seeds each year. Suchhuge demand for seeds has made the Chinese seed market more and more attractive for investment. Through analysis on the present situation and existing problems of the seed industry in China and based on the current Chinese seed industry development, some future prospects for investments are indicated. This investigation was carried out to propose the appropriate strategies on the development of the Korea seed industry as it considers its entry into the China seed market as a new growth engine in the agricultural sector. The basic law regulating the Chinese seed industry is the PRC Seed Law that generally refers to the protection of germplasm resources, verification of varieties, seed quality issues, the import and export of seeds, seed administrative management, and various rights and obligations. The regulations were aimed at the protection of the rights concerning new varieties of plants. China has two main industry associations, the National Seed Association and the China Seed Industry IP Union, that are non-profit associations consisting of entities and people engaging in the seed scientific research, production, operation and management. The China National Seed Group Co., Ltd. ("Sino Seeds") is the market leader in China regarding the seed industry. The chinese government, however, encourages investment from multinational companies as well as importation of modern crop planting management technologies and equipment. It supports the entry of investors with proven experiences in breeding and germplasm resources expansion and R&D. There has never been a better time for multinational companies with proven seed industry experience to look at building relationships with the Chinese government and enterprises.
연구논문 : 자연과학 ; 한국과 불가리아 재래종 강낭콩 Phaseolus vulgaris L. 유전자원의 형태적 특성 및 SSR 다양성 비교
최유미 ( Yu Mi Choi ),이정로 ( Jung Ro Lee ),이기안 ( Gi An Lee ),조규택 ( Gyu Taek Cho ),정연주 ( Yeon Ju Jeong ),( T Stoilova ),박용진 ( Yong Jin Park ),이명철 ( Myung Chul Lee ),이정란 ( Jeong Ran Lee ) 한국국제농업개발학회 2012 韓國國際農業開發學會誌 Vol.24 No.2
본 연구는 농업유전자원센터에 보존되어있는 우리나라 및 불가리아 재래종 강낭콩 유전자원 130점을 대상으로 형태적 특성을 조사하고, 155점에 대해 분자마커를 이용하여 우리나라와 불가리아 재래종 강낭콩 유전자원의 유전적 다양성을 분석하였으며 결과는 다음과 같다. 1. 개화일수는 최소 50일에서 최대 79일, 평균 54.5일이었으며 성숙일수는 최소 25일, 최대 64일, 평균 38.9일 이었고 생육일수는 최소 83일, 최대 123일, 평균 93.4일이었다. 불가리아 재래종 자원들이 한국 재래종 자원보다 개화일수는 3.1일, 성숙일수는 3.6일, 생육일수는 6.7일 늦은 것으로 조사되었다. 2. 공시자원의 100립중은 19~71g 사이에 분포하였고, 평균 100립중은 41.2 g이었으며, 우리나라 재래종 자원은 평균 100립중이 40.9 g, 불가리아 재래종 자원은 42.4 g 이었다. 3. 조사대상 자원의 질적형질 중 생육습성은 한국 자원은 직립형이 87.6%인 반면 불가리아 자원은 덩굴성이 60.0%였으며, 한국 자원의 화색은 보라색 줄무늬가 있는 백색이 80%로 가장 많았으나 불가리아 자원은 백색자원이 52%를 차지하였다. 종피색은 한국 자원은 적색이 61%, 불가리아 자원은 백색이 48%를 차지하여 국가간 선호도와 용도에 따른 차이가 있을 것으로 보였다. 4. 10개의 SSR 마커를 이용하여 다양성을 분석한 결과 PIC 0.6181을 얻었으며, 국가별 분석 결과 다양성은 gene diversity와 PIC로 볼 때 불가리아 자원이 다양성이 높은 것으로 조사되어 불가리아에서 도입한 강낭콩 유전자원이 강낭콩 수집단의 유전적 다양성 증대에 기여한 것으로 평가된다. The morphological traits and the genetic diversity of 130 common bean landraces from Korea and Bugaria were evaluated. Days from sowing to flowering were ranged from 50 to 79 days with an average of 54.5 days. Days from flowering to maturing were ranged from 25 to 64 days with an average of 38.9 days, and days from sowing to maturing were ranged from 83 to 123 days with an average of 93.4 days. Common beans originated from Korea tend to bloom and mature earlier than those from Bulgaria. In growth habit, 87.6% of being originated from Korea are erect type, but 60.0% from Bulgaria are climbing type. Also qualiitative characteristics of both countries were different from each other. For example, 80% of the colors of flower were white with purple stripe in Korea, but 52% of those were white in Bulgaria. One hundred fifty-five common beans from Korea and Bulgaria were analyzed using 10 SSR markers. Ninety-seven alleles were detected with a lowest five at the BM161, BM181, and BM211 and a highest 18 at the BM156. The average polymorphism information content (PIC) was 0.61. Genetic diversity was higher in the Bulgarian population than that of Korea.