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신경성장촉진 인자가 인간 배아줄기세포 유래 도파민 분비 신경세포형성에 미치는 영향
이금실,김은영,신현아,조황윤,왕규창,김용식,이훈택,정길생,이원돈,박세필,임진호,Lee, Keum-Sil,Kim, Eun-Young,Shin, Hyun-Ah,Cho, Hwang-Yoon,Wang, Kyu-Chang,Kim, Yong-Sik,Lee, Hoon-Taek,Chung, Kil-Saeng,Lee, Won-Don,Park, Se-Pill,Lim, Jin 대한생식의학회 2004 Clinical and Experimental Reproductive Medicine Vol.31 No.1
Objective: This study was to examine the in vitro neural cell differentiation patterns of human embryonic stem (hES) cells following treatment of various neurotrophic factors [basic fibroblast growth factor (bFGF), retinoic acid (RA), brain derived neurotrophic factor (BDNF) and transforming growth factor (TGF)-$\alpha$], particulary in dopaminergic neuron formation. Methods: The hES cells were induced to differentiate by bFGF and RA. Group I) In bFGF induction method, embryoid bodies (EBs, for 4 days) derived from hES were plated onto gelatin dish, selected for 8 days in ITSFn medium and expanded at the presence of bFGF (10 ng/ml) for another 6 days followed by a final differentiation in N2 medium for 7, 14 and 21 days. Group II) For RA induction, EBs were exposed of RA ($10^{-6}M$) for 4 days and allowed to differentiate in N2 medium for 7, 14 and 21 days. Group III) To examine the effects of additional neurotrophic factors, bFGF or RA induced cells were exposed to either BDNF (10 ng/ml) or TGF-$\alpha$ (10 ng/ml) during the 21 days of final differentiation. Neuron differentiation and dopamine secretion were examined by indirect immunocytochemistry and HPLC, respectively. Results: The bFGF or RA treated hES cells were resulted in similar neural cell differentiation patterns at the terminal differentiation stage, specifically, 75% neurons and 11% glial cells. Additionally, treatment of hES cells with BDNF or TGF-$\alpha$ during the terminal differentiation stage led to significantly increased tyrosine hydroxylase (TH) expression of a dopaminergic neuron marker, compared to control (p<0.05). In contrast, no effect was observed on the rate of mature neuron (NF-200) or glutamic acid decarboxylase-positive neurons. Immunocytochemistry and HPLC analyses revealed the higher levels of TH expression (20.3%) and dopamine secretion (265.5 $\pm$ 62.8 pmol/mg) in bFGF and TGF-sequentially treated hES cells than those in $\alpha$ RA or BDNF treated hES cells. Conclusion: These results indicate that the generation of dopamine secretory neurons from in vitro differentiated hES cells can be improved by TGF-$\alpha$ addition in the bFGF induction protocol.
의복의 신분상징성과 사회심리학적 변인과의 상관연구 -서울의 가정주부를 중심으로-
이금실,강혜원,Lee Keum Sil,Kahng He Won 한국의류학회 1978 한국의류학회지 Vol.2 No.2
The purpose of this study was to investigate the relationship between clothing interests in the status symbol and the exhibition need and status inconsistency. The exhibition need was measured by Murray's statements on need and some items selected from The Need Diagnosis Scale of Jaung-Kyu Whang. Status inconsistency was measured by the same criteria of Lenski. Three aspects of clothing interests-the status symbol, the fashion and the exhibitionism-were assessed. The questionnaires were distributed to the housewives in Seoul. Data from 511 respondents were analyzed by correlations and $x^2$-tests. The results were: 1) The exhibition need was positively related to interests in the status symbol and exhibitionism of clothing. 2) Status inconsistency was positively related to clothing interests in the status symbol and fashion. 3) The degree of clothing interests in the status symbol and fashion were also positively related to the clothing expenditure and the socioeconomic status of the sample.