동결 절편에 있어서 Dimethyl Sulfoxide가 미치는 영향

윤인숙 대구보건대학 1992 대구보건대학 論文集 Vol.13 No.-

On the basis of the fact which Dimethyl sulfoxide(DMSO) penetrate tissue rapidly and reduce the size of ice crystals, a test was done to know whether DMSO can be used in the process of frozen sections. Small blocks of liver, Kidney, lung and spleen tissues were dissected form rats and were divided each into four portions. One block of each tissue was placed for 20 to 25 minutes in each of the following solutions : normal saline(NS), 5%(V/V) DMSO in NS, 10% DMSO in NS, and 20% DMSO in NS. Frozen sections were done on these tissues, and then hematoxylin-eosin stain was done and compared. Generally there was less tissue disruption in the samples treated with DMSO than without DMSO. Especially, in all the tissues, the 10% DMSO gave the best results in staining, cutting and the thickness of the tissue sections. Tissue treated with 20% DMSO was very hard to cut and could not be cut as thinly as the 5% or 10% DMSO treated tissue. Especially in the liver tissue, shrinkage of tissue sections was occured. The kidney tissues treated with 5% DMSO and 10% DMSO were comparable in reducing tissue disruption. The DMSO does not interfere with formalin fixation after frozen sections in the process of routine preparation. The time of penetration of the DMSO into the tissue block was very fast and penetration was apparently completed by ten minutes. If the time of treatment with DMSO was longer, it stained darkerly with hematoxylin-eosin. All of these findings suggest that treatment with 10%(V/V) DMSO for frozen sections enhances the quality of the frozen sections. If possible, this method should be used for human pathologic specimens through the examination on applying DMSO to human tissues.

흰쥐에게 Ethanol과 Xylene 병행 투여시 간손상에 미치는 영향

윤인숙(In-Sook Yoon),이혜자(Hye-Ja Lee) 한국콘텐츠학회 2010 한국콘텐츠학회논문지 Vol.10 No.7

흰쥐에게 ethanol과 xylene을 투여시 간손상 정도를 알아보기 위해 정상적인 S-D계 수컷 흰쥐를 4군으로 나누어 실험을 수행하였다. 생화학적 검사 결과에서 혈청 AST, ALT, LDH활성은 대조군보다 ethanol 투여군, xylene투여군, ethanol+xylene투여군으로 갈수록 활성이 유의하게 증가되었으며, 그 증가율은 ethanol+xylene투여군에서 가장 높게 나타났다. 산화적 손상정도를 알아보기 위한 혈청 MDA함량은 대조군보다 ethanol투여군과 xylene투여군 그리고 ethanol+xylene투여군으로 갈수록 활성이 증가되었으며, 그 증가율은 ethanol+xylene투여군에서 가장 높게 나타났다. 그러나 혈청 ALP는 대조군에 비해 모두 감소하였다. Xylene의 투여시 최종대사산물인 요 중 m-methylhippuric acid는 대조군에 비해 xylene투여군과 ethanol+xylene투여군에서 증가되었으며, 그 증가율은 xylene투여군이 ethanol+xylene투여군보다 더 높게 나타났다. 간 조직검사에서는 xylene투여군은 지방변화를, ethanol+xylene 투여군은 경도~중등도의 괴사와 염증을 보였다. 이상의 혈청학적 검사와 조직학적 검사결과 xylene 단독 투여에 비하여 ethanol과 xylene을 병행 투여했을 때 간손상이 더 크게 초래된 것으로 나타났다. To evaluate an effect of liver damage on ethanol and xylene exposure, experiments on normal male rats of the S-D strain were performed in 4 groups. The biochemical results suggest that the ethanol group had significantly higher levels of AST, ALT, LDH and also, the xylene group had notably higher levels of AST, ALT, LDH along with MMHPA than those of the control groups. The levels of AST, ALT and LDH in the ethanol+xylene group were drastically higher than those in the control, ethanol and xylene groups. But, there were significantly lower ALP levels in the xylene and ethanol+xylene groups than both the control and ethanol groups. The histological features of rat livers treated with alcohol, or xylene proved to be normal. But the rat livers treated with ethanol+xylene showed mild to moderate necrosis and inflammation as well as minimal fatty changes. The results in this experiment suggest that liver functions decreased when medicated together with xylene and ethanol rather than solely with xylene.

혈액형에 따른 여가활동 유형 -대구보건대학 보건계열 및 비보건계열 학생들을 중심으로-

윤인숙 ( In Sook Yoon ),김혜정 ( Hye Jong Kim ) 대한임상검사과학회 2006 대한임상검사과학회지(KJCLS) Vol.38 No.1

We examined the relationship between the basic characteristics and types of leisure activities according to blood types. It was based on questionnaires answered by 441 collegians in public health departments and 282 in non-public health departments in Daegu Health College in March, 2005. The frequency of leisure activity of once or twice a week or less during weekdays was the most common.: The most common frequencies for all blood types were between 1 and 2 hours on weekdays and between 3 and 5 hours on the weekends or holidays. As far as the type of leisure activities during the weekdays was concerned, in the case of public health departments, blood type A was interested in computer games and then watching TV, blood type B was attracted to watching TV and listening to music, blood type AB was into watching TV, computer games, and listening to music, and blood type O was keen on watching TV and computer games in that order. The type of leisure activity according to blood types in public health departments was shown to be significantly different (p<0.05). The type of future leisure activity was shown to be significantly different between public health departments and non-public health departments (p<0.05). The type of present leisure activity preferred by collegians, according to the study, was entertainment such as computer games for most blood types. The type of future leisure activities was related to the field of tourism and sports. The collegians need to have more advanced and positive leisure activities.

마늘추출물의 식중독균에 대한 항균검사

윤인숙(In-Sook Yoon) 한국콘텐츠학회 2009 한국콘텐츠학회논문지 Vol.9 No.2

마늘은 오랫동안 그 가치가 높이 평가되고 있으며 약용, 항균 및 살균효과적인 특성으로 인해 실제로 사용되어오고 있다. 본 연구는 마늘추출물의 천연식품보존제 개발가능성을 알아보기 위해 수행되어졌으며 신선한 마늘추출물과 이를 증류수로 희석한 마늘추출물들을 사용하여 6종의 식중독균 즉, S. aureus, E. coli, S. typhimurium, V. parahemolyticus, B. subtilis, L. monocytogenes들에 대한 감수성검사를 실시하였고 그 결과를 그람양성균 치료에 사용되는 Vancomycin과 그람음성균 치료에 사용되는 Imipenem을 대조로하여 이들 균주에 대한 항균력과 비교하였다. 마늘추출물에서 6균주 모두에 대해 강한 감수성을 보이거나 혹은 중등도의 감수성을 보였는데 V. parahemolyticus만은 마늘추출물의 원액에서만 항균력이 나타났고 희석한 농도에서는 항균력이 없었다. Garlic (Allium sativum, L.) has a long history of reputed value and actual use for its medicinal, antimicrobial, and pesticidal properties. This study was conducted to find possible developments to natural food preservatives from garlic extracts. Garlic extracts were prepared from fresh crushed garlic and diluted with sterilize distilled water. The sensitivity test was conducted to investigate the antimicrobial effect of garlic extract against six strains of food poisoning bacteria such as Staphylococcus aureus, Escherichea coli, Salmonella typhimurium, Vibrio parahemolyticus, Bacillus subtilis, and Listeria monocytogenes. The results of sensitivity test were then compared with those of Vancomycin and Imipenem which were used as treatment for G. (+) bacteria and G. (-) bacteria, respectively. All of the 6 tested strains exhibited strong or moderate activity. V. parahemolyticus exhibited susceptible only in undiluted solution, but not in diluted garlic extracts.

면역조직화학적 염색 방법에 따른 상피세포 성장 수용체 단백(EGFR)의 발현정도의 차이 및 EGFR의 발현정도와 EGFR 유전자의 돌연변이와의 상관관계에 대한 고찰

윤인숙 ( In Sook Yoon ),김극준 ( Keuk Jun Kim ),이은화 ( Eun Hwa Lee ),석상희 ( Sang Hee Seok ),김상희 ( Sang Hee Kim ),김현용 ( Hyun Yong Kim ),송호정 ( Ho Jung Song ),이태종 ( Tae Jong Lee ) 대한임상검사과학회 2007 대한임상검사과학회지(KJCLS) Vol.39 No.3

In the last 5 years the Epidermal Growth Factor Receptor (EGFR) has emerged as one of the most important targets for drug development in oncology. Monoclonal antibodies targeting the external domain of EGFR have been shown to have clinical benefits in colorectal and head and neck cancer when combined with chemotherapy and/or radiation. Also the targeting of the epithelial growth factor receptor (EGFR) kinase domain using the closely related inhibitors gefitinib and erlotinib has generally been ineffective against solid tumors, many of which over express the receptor. We found that there were some differential expressions according to primary antibodies of the EGFR protein which being used as one of the histological tumor markers for non-small cell lung cancer (NSCLC). We also found that there are some differential expressions according to antibodies, the pH of the antigen retrieval (AR) buffer solutions and kinds of enzymes. There were some differential expressions according to the secondary antibodies and the detection systems. We analyzed the correlations between the immunohistochemical expressions of the EGFR protein and the gene mutations of the EGFR. The differences between automatic stainers and manual staining methods were also evaluated.

The Effect on the Liver Function in Rats Treated with Xylene and Ethanol

윤인숙 ( In Sook Yoon ) 대한임상병리사협회 2005 조직세포검사학회 발표자료집 Vol.2005 No.-

Background : Xylene is an aromatic hydrocarbon that has a ring. It is included in various materials such as paint, plastic, rubber paste, aircraft fuel, diluent, printing solvent. However, xylene is relevant to medical technologists because of its use in making slides for biopsy in hospital laboratories. Xylene is mainly metabolized in the liver via the oxidative pathway. There are reports that xylene can bring about damage to the liver, nervous system, and hematogenesis when it is exposed in humans. Ethanol is human favorite food but it is the most frequently ingested toxin throughout the world. Nevertheless, the high amount of alcoholic consumption said to cause the improvement of the living standard, which allows us more leisure time as well as reducing occupational stress. So, the effects of alcohol on liver function were studied, the group being that of laborers who handle xylene. Methods : Normal male rats of the Sprague-Dawley strain, weighing between 320g and 350 g, were used in this experiment. All the rats were fed on a standard rat chow as well as water. Experiments were performed in four groups; A Control Group, an Ethanol Group, a Xylene Group, and an Ethanol + Xylene Group. Urine specimens were collected in metabolic cage for 24 hours on the last day of receiving xylene injection. Blood samples were obtained from the abdominal aorta under light ether anaesthesia. AST, ALT, LDH and ALP levels in the serum were measured to estimate the levels of liver function. MDA, which gives an indirect index of oxidative injury was also measured. MMHA which is the last metabolic production of xylene was measured with HPLC. Liver tissues were resected and fixed in 10% formalin, stained with H&E. Results : The biochemical results suggest that the Ethanol Group had significantly higher levels of AST, ALT and LDH than those of the Control Group. Also, the Xylene Group had notably high levels of AST, ALT, LDH as well as high levels of MMHA than those of the Control Group. The levels in the Ethanol + Xylene Group was drastically higher than both the Control and Ethanol Groups. Also, the said levels in the Ethanol + Xylene Group were significantly higher than those of the Xylene Group. But, there were significantly lower ALP levels in the Xylene Group and Ethanol + Xylene Group than both the Control and Ethanol Groups. The Ethanol + Xylene Group had significantly higher levels of AST, ALT and LDH than those of the Xylene Group. The histological features of the rat livers treated with alcohol, or xylene showed normal. But the rat livers treated with alcohol + xylene showed mild to moderate necrosis and inflammation, and minimal fatty changes. Conclusions : To conclude the experiment on liver function of rats with ethanol intoxication combined with xylene hepatotoxicity, one can generally state that the Ethanol + Xylene Group had worsened liver function, more so than that of the Xylene Group. Accordingly, the results in this experiment suggest that liver function is far worse when medicated together with xylene and ethanol rather than medicated xylene according to the rat experiment model. Therefore, laborers who handle xylene should seriously consider health aspects before consuming alcohol, or at least control their often heavy drinking habits. HTs must be protected from harmful environments which expose them to xylene and ethanol. The histology room must be standardized with modern ventilation equipment at the standpoint of the Ministry of Environment or the Ministry of Health and Welfare.

증례(症例) : Pemphigus Vulgaris의 세포학적, 조직학적 그리고 임상적 상호관계

윤인숙 ( In Sook Yoon ) 대한임상검사과학회 1983 대한임상검사과학회지(KJCLS) Vol.15 No.1

A case of pemphigus vulgaris is reported with a review of previous literatures. The only case selected for this study was that in which the clinical, histologic and cytologic diagnoses were in agreement. The patient showed an oral manifestation, which appeared 9 months ahead of the cutaneous lesions. In Mar. 1980 the patient became aware of a few intraoral lesions. She went to the pharmacy and was give medication. But her intraoral lesions continued to spread. From several weeks prior to admission, she developed some blisters -around her external genitalia. A few days later numerous bullous lesions appeared on her back, axilla, scalp, neck, extremities and in the midface around her eyes. She was admitted to the hospital on the 28th. of Dec. 1980, at ,which time a skin biopsy and smears were taken from the erupted bullae on her left thigh. The biopsy was interpreted as consistent with the tissue of pemphigus vulgaris and the smears showed cellular changes which are compatible with pemphigus. I want to clarify here that the clinical information was derived from the archives of the recorded patients` documents.

중국 연변 조선족 여성의 자궁경부세포 검사소견

윤인숙 ( In Sook Yoon ),이혜자 ( Hye Ja Lee ) 대한임상검사과학회 2003 대한임상검사과학회지(KJCLS) Vol.35 No.2

According to literature, the risk of developing cervical invasive carcinoma is at least 10 times greater in women not screened by a cervical Papanicolaou smear than in women screened by the same test. We performed cervical Pap smears to investigate the causes and rate of cervical cancer in Yanbian Korean-Chinese women who could not receive the Pap test until now. We administered a questionnaire concerning epidemiological factors of cervical cancer and a free cervical Pap smear was given. It was administered three times from April 2000 to July 2001 for the purpose of producing basic data concerning the management of cervical cancer in Yanbian Korean-Chinese women. Women who had previously received Pap smear tests comprised of 3.6% or 25 out of every 704 women in Yanbian. The results of the Pap smears in the 704 women showed two squamous cell carcinomas(0.3%), 14 LSILs(2.0%), 20 ASCUSs(2.8%), 390 inflammatory changes(55.4%) and 278 negatives(39.5%). Of special note, the HPV infection rate of the Yanbian Korean-Chinese women was recorded as 1.7%(12 persons) which is higher than any other rate found in the literature. I will therefore continue the investigation of cervical HPV typing.

알레르기 환자에서 Immunoglobulin E와 Eosinophil count 및 Allergen과의 관련성

윤인숙(In-Sook Yoon),권세영(Se-Young Kwon) 한국콘텐츠학회 2009 한국콘텐츠학회논문지 Vol.9 No.4

알레르기 환자의 증가로 인하여 알러겐을 찾는 것은 중요하다. MAST는 알러겐 특이 IgE 항체를 측정하는 간단한 방법이다. 본 연구에서는 알레르기 환자에서 알러겐 특이 IgE 항체, 총 IgE, 호산구수와의 관련성을 평가하고자 하였다. 연구 결과 총 IgE 양성률은 흡입형 패널에서 96.97%, 식품형 패널에서 98.06% 였다. 알러겐 특이 IgE 항체의 양성률은 흡입형 패널에서 집먼지 51.52%, 북아메리카 집먼지 진드기 45.46%, 고양이 31.99% 였으며, 식품형 패널에서 각각 55.34%, 42.72%, 34.96%였다. 혈청 총 IgE는 알레르기와 관련이 있었으나, 알레르기가 항상 호산구증가와 관련이 있는 것은 아니었다. As the allergic diseases in patients are increasing, it is important to find out the allergens. A multiple antigen simultaneous test(MAST) is a simple method for in vitro measurement of allergen-specific IgE antibodies. This study was performed to evaluate the relationship between allergen-specific IgE antibodies, serum total IgE and peripheral eosinophil count in the allergic patients. According to the results of the study, the total IgE positive rate(above class 2) from the inhalent is 96.97%, and that from food panel is 98.06%. The research showed that the positive rate of the allergen-specific IgE was House dust 51.52%, D. farinae 45.46%, Cat 31.99% in inhalent panel, and 55.34%, 42.72%, 34.96% in food panel. Serum total IgE was associated with allergy, however, allergy was not always associated with eosinophilia.

서울시 지역균형발전 전략으로서의 뉴타운개발사업의 한계

윤인숙(In Sook Yoon),김갑성(Kab Sung Kim) 한국지역학회 2004 지역연구 Vol.20 No.2