3D 프린터를 활용한 로봇 팔의 제작과 제어를 위해 시도한 융합 교육의 발전 방안 연구

장은영 ( Eum-young Chang ),유형진 ( Hyung-jin Yu ) 한국실천공학교육학회 2024 실천공학교육논문지 Vol.16 No.2

이 연구는 특성화고 학생들에게 인벤터 소프트웨어를 사용하여 로봇 팔을 디자인하고 3D 프린터를 통해 제작하여 아두이노 마이컴으로 제어하는 융합 교육 방법을 소개하며, 학생들에게 실무 경험을 제공하고 다양한 학문 분야의 지식과 기술을 융합할 수 있는 기회를 제공할 수 있다. 학생들은 CAD 소프트웨어로 디자인을 시작하고, 3D 프린팅 기술을 통해 실제로 로봇 팔의 부품을 제작하며, 마지막으로 부품을 조립한 로봇 팔을 프로그래밍하여 제어하게 하였다. 이 융합교육에서 학생들의 문제 해결 능력과 창의성을 키우고, 학습 동기를 높이는 데 도움이 되었고 이런 교육적시도가 실제 교육과정에 적용하기 위해서는 적절한 자원과 교사의 지속적인 관심과 지원이 필요하며, 이러한 연구는 향후 교육에서 적용 가능성을 탐색하기 위한 기초적인 연구로 간주된다. This study introduces specializer high school students , as a fusion education method using Inventor software to design a robot arm, which is then 3D printed and controlled by an Arduino microcontroller. Students gain practical experience and have the opportunity to integrate knowledge and skills from various academic fields. They start by designing in CAD software, proceed to fabricate actual robot arm components using 3D printing technology, and finally program and control the assembled robot arm. This interdisciplinary education enhances students’ problem-solving abilities, fosters creativity, and increases their motivation to learn. To implement such educational endeavors in actual curricula, ongoing teacher support and appropriate resources are essential. This research serves as a foundational exploration of the applicability of fusion education in future learning contexts.

열분해 방식에 따른 고체 커패시터의 특성연구

김재근 ( Jae Kun Kim ),유형진 ( Hyung Jin Yu ),홍웅희 ( Woong Hee Hong ) 한국화학공학회 2006 Korean Chemical Engineering Research(HWAHAK KONGHA Vol.44 No.6

알루미늄 고분자 콘덴서의 특성에 대한 절연지 탄화의 영향

김재근 ( Jae Kun Kim ),유형진 ( Hyung Jin Yu ),홍웅희 ( Yoong He Hong ),박미진 ( Mi Jin Park ),박승열 ( Seung Youl Park ) 한국공업화학회 2006 공업화학 Vol.17 No.5

산화중합에 의한 Polypyrrole 적용 탄탈륨 콘덴서의 특성에 관한 연구

김재근(Jae Kun Kim),민혜경(Hye Kyong Min),유형진(Hyung Jin Yu),강기혁(Ki Hyeck Kang) 한국공업화학회 2001 공업화학 Vol.12 No.6

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Pyrroloquinoline quinone(PQQ)에 特異的인 抗體의 生産

마병철,유형진,권무식 성균관대학교 생명과학자원연구소 1997 生命資源科學硏究 Vol.4 No.1

There exist biocatalysts(e.g., some oxidoreductase and dehydrogenase) containing pyrroloquinoline quinone (PQQ) as a cofactor. They are physiologically and pathogenically very important, since the quinoproteins are involved in various cellular activities, such as stimulation of cell growth, pollen germination, protective effects against hepatotoxin-induced liver injury, and so on. It is, therefor, interesting to develop a sensitive method to identify the quinoproteins in organisms. Monospecific polyclonal antiserum specific to PQQ was raised in a rabbit as follow. The PQQ was conjugated to bovine serum albumin (BSA) by 1-Ethyl-3-(3-dimethyl-aminopropyl)carbodiiminde HCl (EDC) mediated reaction. The PQQ-BSA conjugate was purified by gel filtration. The conjugate concentration was measured by Bradford method. The conjugates was emulsified with adjuvant. The emulsion was hyperdermally injected to a rabbit. PQQ-lipase conjugate was made to use as an antigen for the anti PQQ-BSA conjugate antiserum. The titre of the antiserum was determined by indirect solid phase immunoassay. The antiserum showing the highest titre recognised nano-gram quantity of PQQ-lipase conjugate.

Amylase 特異的인 抗體의 生産

강미란,이정민,유형진,김기윤,권무식 성균관대학교 생명과학자원연구소 1997 生命資源科學硏究 Vol.4 No.1

The advent of immunochemistry expedites the development of a number of important procedures for gene cloning, disease control (diagnosis, treatment) and biochemical analyses in animal systems. These procedures can be applicable to plant systems : immunochemical detection of biopolymers (proteins, carbohydrates, growth hormones, and pathogenic virus particles). It is not quite difficult to purify various palnt immunogens. However, immunochemical screening methods for specific substances of plants are scarce, so that these procedures for each substance are to be developed. During the cereal germination, amylase converts the starchy endosperm of the seed to nutrient that support the growing seedling. α-Amylase and β-amylase hydrolyze internal α-1,4 glycosidic linkages in starch and dextrin. They produce α-maltose and β-maltose respectively. Their gene expression is regulated by gibberellic acid (negative regulator) and abscisic acid (positive regulator). In this study, α-amylase (from barley malt) protein was analyzed with SDS-PAGE. The molecular weight of α-amylase was about 60,000 daltons. α-Amylase protein was used as immunogen to produce antiα-amylase antiserum in a rabbit. The immunogen was mixed with an equal volume of Freund's complete adjuvant, and the mixture was hypodermically injected to the hideback (30 spots) and paws (2 spots) of the rabbit. The immunization was performed with two week interval. The dosage of the immunogen was decreased by half, and incomplete adjuvant was substituted for the complete one from the second immunization. The serum was prepared from the blood, and immunoreactivity of the serum was examined by dot-blotting and western blotting using the GARHRP indirect immunoassay kit. It was found that the anti α-amylase antiserum recognized nano-gram quantity of antigen under these experimental conditions. The antiserum will be used for the development of immunochemical assay to detect the time of the seed germination.

殺蟲性 結晶蛋白質 特異的인 抗體의 生産

김기윤,이정민,유형진,백길현,권무식 성균관대학교 생명과학자원연구소 1996 生命資源科學硏究 Vol.3 No.1

Bacillus thuringiensis is a gram-positive soil bacterium characterized by its ability to produce crystalline inclusions during sporulation. These parasporal bodies consist of protoxins known as crystal proteins exhibiting highly specific insecticidal activities. The Lepidoptera-specific protoxin, Cry IAc, was purified from E.coli JM103 harboring the cry IAc gene. The gene was isolated from Bacillus thuringiensis kurstaki HD-73 and subcloned into an expression vector, pKK223-3. The recombinant DNA(pOS4201) was transformed into E.coli JM103. The Cry I Ac protoxin overexpressed in the E.coli was isolated from the total proteins by differential solubility. The protoxin were trypsinized to obtain activated toxin. The proteins was resolved on SDS gel. The molecular weights of the pro-and toxin were resistered about 130,000 and 65,000 Daltons respectively. The Cry IAc toxin was used as immunogen to produce anti-Cry IAc antiserum in a rabbit. The immunogen (200㎍/ 200㎕) was mixed with an equal volume of Freund's complete adjuvant, and the mixture was hypodermically injected to the hide back (30 spots) and paws (2 spots) of the rabbit. The immunization was performed four times every two weeks. The dosage of the immunogen was decreased by half, and incomplete adjuvant was substituted for the complete one from the second immunization. The serum was prepared from the blood as described elsewhere. Immunoreactivity of the serum was examined by dot-blotting with the aid of GAR-HRP indirect immunoassay kit. It has been found that the anti-Cry I Ac antiserum recognized nano-gram quantity of antigen under these experimental conditions. The antiserum will he used for the development of immunochemical mean(s) to screen transgenic plants transformed by the insecticidal characteristics of Cry I Ac.

殺蟲性 結晶蛋白質(Cry ⅡA) 特異的 抗體 生産

이정민,유형진,김기윤,백길현,권무식 성균관대학교 생명과학자원연구소 1996 生命資源科學硏究 Vol.3 No.2

Bacillus thuringiensis, a gram-positive soil bacterium, is characterized by its ability to produce crystalline inclusions during sporulation. The crystal proteins exhibit a highly specific insecticidal activity. A crystal protein, Cry Ⅱ A, is specifically toxic to both lepidopteran and dipteran insects. Scientists are working hard to generate the insect-resistant transgenic plants due to the instability and low-persistence of the proteins. In this study, to confirm the expression of the cryⅡA gene, anti-CryⅡ A antibody was raised in a rabbit. The Cry Ⅱ A crystal protein was purified from E. coli JM103 harboring the cry ⅡA gene byc differential solubility. The Cry Ⅱ A was digested with trypsin for the activation. Some 300 ㎍ of the activated Cry Ⅱ A was mixed with the same amount of FCA, and the slurry was hyperdermally injected onto about 20 spots of the back, one spot of the thigh, and one spot of the footpad of the rabbit. The immunization was performed four times with two weeks interval. From the third immunization, FIC was substituted for the FCA. The rabbit serum was collected from the ear vein, and anti-Cry Ⅱ A antibody was purified by Protein A affinity chromatography. The anti-Cry Ⅱ A antibody recognized nanogram quantity (1.25 ng) of Cry Ⅱ A by solid phase immunoassay. So it can be available for screening of transgenic plants with the insecticidal characteristics of Cry Ⅱ A.

배추에서 암(暗)조건 발아 유전자의 ESTs 제작

권무식,류종석,김재범,유형진 한국유전학회 1997 Genes & Genomics Vol.19 No.3

Expressed sequence tags (ESTs) are short cDNA sequences generated from randomly selected library clones. ESTs are widely used to clone genes and/or to elucidate their structures and/or functions. We generated a set of ESTs in Chinese cabbage which is an important vegetable species in Korea. Fifty ESTs were generated from the Chinese cabbage (Brassica campestris L. var. pekinensis). Poly A^+ RNAs were isolated from 10-day-old seedlings germinated in dark and two cDNA libraries were constructed by employing λ ZAP-cDNA synthesis system (Stratagene, USA). Then, clones from the libraries were selected randomly and were sequenced by the Sanger method with manual or automated DNA sequencing apparatus. Comparing the ESTs with the coding sequences of the previously reported protein in GenBank or EMBL, significant levels of similarity were found in the amino acids or nucleotide sequences. However, some ESTs showed low level of similarity with the sequences in GenBank or EMBL, which could represent novel genes. Therefore, the reported sequences and cDNA libraries in this study could be utilized to clone more genes in Brassica in the future.