동빈문고의 中國飜刻本과 그 가치-「近思錄」과 「韻府群玉」을 중심으로-

옥영정 영남대학교 민족문화연구소 2012 민족문화논총 Vol.51 No.-

This paper that is bibliographical study of the Book Culture of East Asia deals with value and meaning of Geunsarok and Unbugunok belonging to Dongbin Collection of Yeongnam University and printed in late Goryeo and early Joseon. In other words, I proceeded to study focusing on Geunsarok and Unbugunok belonging to Dongbin Collection of Yeongnam University which had been reprinted in Joseon in the process of importing Chinese edition printed in Song-Yuan Dynasty. Examining the real books, I inquired into pattern and circulation of the book exchange and found out new materials. Also I think this is meaningful in understanding origin and early form of Korean old books. In case of reprinting Chinese books imported in Joseon, they were reprinted as original forms or printed in new style maintaining original format. Also at first having reprinted as original forms, later they were printed in new layout making use of movable type. Based on Chinese books, Geunsarok and Unbugunok were reprinted as original forms. I consider that the comparison between reprinting of Song-Yuan books and original books remaining in Korea, based on the physical bibliography of Song-Yuan edition, will be helpful in clarifying influence and contents of Chinese books in East Asian Book Culture. 이 연구는 동아시아 서적문화에 관한 서지학적 고찰로 영남대 동빈문고 소장의 중국 번각본 중 고려말과 조선전기에 간행된 「근사록」과 「운부군옥」에 대하여 그 가치와 의의를 다루었다. 송・원시기 중국에서 간행된 서적이 조선에 수용되는 과정에서 조선에서 간행된 번각본인 「근사록」과 「운부군옥」을 중심으로 영남대 동빈문고의 두 책을 대상으로 삼아 연구를 진행한 것이다. 실물자료에 의한 구체적인 사례를 통해 서적 교류의 유형과 유통 및 새로운 자료에 대한 발굴이란 측면에서 연구를 진행하였다. 이는 국내 고문헌의 원류와 초기의 형태를 파악하는 데에도 의의가 있다. 조선에 유입된 중국간본은 간본을 저본으로 하여 그대로 번각하는 경우도 있지만, 본래의 판형을 유지하되 새롭게 중간하는 경우가 있다. 또 처음에는 간본을 그대로 번각하였다가 새로운 활자가 제작되면 해당 활자를 이용하여 새로운 판식으로 간행하기도 하였다. 「근사록」과 「운부군옥」은 저본을 바탕으로 그대로 번각하는 경우의 예로서 그 방식을 잘 보여준다. 송・원간본의 형태서지적 속성을 바탕으로 고려・조선에서 간행된 송・원간본의 번각본들 중에서 현존하는 자료를 파악하여 한국에 남아있는 송・원간본과의 실증적인 예를 통한 확인 작업이 이루어진다면 동아시아의 서적문화에서 중국 간행서적의 영향과 그 내용을 확인하는데 도움이 될 것으로 사료된다.

16세기 퇴계학파의 서당 경영

이남옥 영남대학교 민족문화연구소 2018 민족문화논총 Vol.69 No.-

Like the image in Kim Hongdo's paintings drawn in the 18th century, the Seodang is generally known as an elementary education space. It was not that there was not a Dongmong Seodang(童蒙書堂) which teacher teaches young children. However, in the late Joseon Dynasty, the Seodang showed various forms of existence. In the 16th century Andong(安東) area, the Seodang, which was influenced by Yi Hwang(李滉)'s aspect of Seodang, was erected since he built the Gyesang Seodong(溪上書堂) and Dosan Seodang(陶山書堂). The Toegye school basically settled in a quiet and scenic place, away from the world, and used it as space for self-discipline and education. Yi Hwang, Kim Seongil(金誠一), and Ryu Seongryong(柳成龍), who had been in office, withdrew from the mediation and used the Seodang as for self-discipline and education. By the way, while Yi Hwang and Kim Seongil used the Seodang as a place to teach their disciples, Ryu Seongryong built the Seodang as the main purpose of his discipline. Accordingly, Kim Seongil erected the Seokmun Jeongsa(石門精舍) and the Okbyeong Seojae(玉屛書齋), and Ryu Seongryong ran the Wonji Jeongsa(遠志精舍) and the Okyeon Seodang(玉淵書堂). On the other hand, Jo Mok(趙穆) expanded the Wolcheon Seodang(月川書堂), which was the traditional Jo's family school, and used it as a space for students to learn the basics of their studies. They basically managed the Seodang for self-discipline and education. However, they managed to run in various forms according to their situation, academic tendency and personal preference. 18세기 그려진 김홍도의 풍속화 속 이미지처럼 일반적으로 서당은 훈장에 의한 초등교육 공간으로 알려져 있다. 훈장이 어린아이들을 가르치는 동몽서당이 없었던 것은 아니지만 조선후기 서당은 이보다 다양한 존재 양상을 보인다. 이황이 계상서당과 도산서당을 건립하여 강학한 이래로 16세기 안동 지역에서는 그의 서당관에 영향을 받은 서당이 건립되었다. 퇴계학파는 기본적으로 세속으로부터 떨어져 조용하고 풍광 좋은 곳에 서당을 세워 藏修講學處로 활용하였다. 관직 생활을 한 이황ㆍ김성일ㆍ류성룡은 모두 조정에서 물러나 강학처로 서당을 활용하였는데, 이황과 김성일이 문인에게 학문을 전수하는 공간으로 서당을 활용한 반면, 류성룡은 자신의 수양을 주요 목적으로 서당을 건립하였다. 이에 따라 김성일은 석문정사와 옥병서재를, 류성룡은 원지정사와 옥연서당을 경영하였다. 한편, 조목은 기존의 가숙서당인 월천서당을 확장하여 문인들에게 학문의 기본을 다지는 공간으로 활용하였다. 이들은 기본적으로 도학형 서당을 경영하였으나, 각자의 상황과 학문적 성향 및 개인적 취향에 따라 다양한 형태로 서당을 경영하였다.

동결 보호제(DMSO) 농도에 따른 돼지 중간엽 줄기세포의 Caspase 3과 7 발현

옥선아,노규진 韓國受精卵移植學會 2012 한국동물생명공학회지 Vol.27 No.3

Adult stem cell transplantation has been increased every year, because of the lack of organ donors for regenerative medicine. Therefore, development of reliable and safety cryopreservation and bio-baking method for stem cell therapy is urgently needed. The present study investigated safety of dimethyl sulfoxide (DMSO) such as common cryoprotectant on porcine bone marrow derived mesenchymal stem cells (pBM-MSCs) by evaluating the activation of Caspase-3 and -7, apoptosis related important signal pathway. pBM-MSCs used for the present study were isolated density gradient method by Ficoll-Paque Plus and cultured in A-DMEM supplemented 10% FBS at in 5% incubator. pBM-MSCs were cryopreserved in A-DMEM supplemented either with 5%, 10% or 20% DMSO by cooling rate at /min in a Kryo 360 (planner 300, Middlesex, UK) and kept into . Survival rate of cells after thawing did not differ between 5% and 10% DMSO but was lowest in 20% DMSO by 0.4% trypan blue exclusion. Activation of Caspase-3 and -7 by Vybrant FAM Caspase-3 and -7 Assay Assay Kit (Molecular probes, Inc.OR, USA) was analyzed with a flow cytometer. Both of cryopreserved and control groups (fresh pBM-MSCs) were observed after the activation of Caspase-3 and -7. The activation did not differ between 5% and 10% DMSO, but was observed highest in 20% DMSO. Therefore 5% DMSO can be possibly used for cell cryopreservation instead of 10% DMSO.

現代 韓國人의 家族主義價値에 대한 研究

玉先花(옥선화) 가정과삶의질학회 1989 가정과삶의질연구 Vol.7 No.2

남방정책의 계보학 : 아세아민족반공연맹부터 신남방정책까지

옥창준(玉昌埈) 새얼문화재단 2019 황해문화 Vol.104 No.-

초급성 거부 반응이 제어된(α1,3-Galactosyltransferase Knock out, α1,3-Gal KO) 형질전환 돼지유래 골수 중간엽 줄기세포의 면역관용능 평가

옥선아,오건봉,황성수,김영임,윤익진,임석기,박진기 한국동물생명공학회(구 한국동물번식학회) 2013 Reproductive & Developmental Biology(Supplement) Vol.37 No.2s

α-1,3-Galactosyltransferase Knock Out(GalT KO) 돼지유래 골수 중간엽 줄기세포의 특성 규명

옥선아,오건봉,황성수,임석기,김영임,박진기 韓國受精卵移植學會 2013 한국동물생명공학회지 Vol.28 No.3

A major barrier to progress in pig to primate organ transplantation or cell therapy is the presence of terminal α -1,3-galactosyl epitopes on the surface of pig cells. Therefore, the purpose of this experiment was to establish and cha- racterize mesenchymal stromal/stem cells (MSCs) derived from α-1,3-galactosyltransferase (GalT) knock out (GalT KO) pig to confirm their potential for cell therapy. Bone marrow (BM)-MSCs from GalT KO pig of 1 month old were isolated by Ficoll-Paque PLUS gradient and cultured with A-DMEM + 10% FBS on plastic dishes in 5% CO2 incubator at 38.5. GalT KO BM-MSCs were analyzed for the expression of CD markers (CD45-, 29+, 90+ and 105+) and in vitro differentiation ability (adiopogenesis and osteogenesis). Further, cell proliferation capacity and cell aging of GalT KO BM-MSCs were compared to Wild BM-MSCs by BrdU incorporation assay (Roche, Germany) using ELISA at intervals of two days for 7 days. Finally, the cell size was also evaluated in GalT KO and Wild BM-MSCs. Statistical analysis was performed by T-test (P<0.05). GalT KO BM-MSCs showed fibroblast-like cell morphology on plastic culture dish at passage 1 and exhibited CD45-, 29+, 90+ and 105+ expression profile. Follow in ginduction in StemPro adipogenesis and osteogenesis media for 3 weeks, GalT KO BM-MSCs were differentiated into adipocytes, as demonstrated by Oilred Ostaining of lipid vacuoles and osteocytes, as confirmed by Alizarinred Sstaining of mineral dispositions, respectively. BrdU incorporation assay showed a significant decrease in cell proliferation capacity of GalT KO BM-MSCs compared to Wild BM-MSCs from 3 day, when they were seeded at 1x103 cells/well in 96-well plate. Passage 3 GalT KO and Wild BM-MSCs at 80% confluence in culture dish were allowed to form single cells to calculate cell size. The results showed that GalT KO BM-MSCs (15.0 ± 0.4 μm) had a little larger cell size than Wild BM-MSCs (13.5 ± 0.3 μm). From the above findings, it is summarized that GalT KO BM-MSCs possessed similar biological properties with Wild BM-MSCs, but exhibited a weak cell proliferation ability and resistance to cell aging. Therefore, GalT KO BM-MSCs might form a good source for cell therapy after due consideration to low proliferation potency in vitro.

이종 장기이식 및 조직 공학을 위한 Alpha gal 유전자 결손돼지 (1, 3-galactosyltransferase-deficient pigs)에서 혈관내피세포(aortic endothelial cells)의 구축

옥선아,임맑음,김영지,ImranUllah,신유리안나,김영임,오건봉,황성수,허태영,이승훈,임기순 한국수정란이식학회 2017 한국동물생명공학회지 Vol.32 No.3

Tissue engineering (TE) has been developed to create functional organs and tissue by combining 3D matrix and cells in vitro. Vascularization and angiogenesis are utmost important for supply of nutrients and oxygen in tissue engineered organs. The present study was performed to isolate and characterize primary endothelial cells (EC) from aorta of alpha 1, 3-enzyme galactosyltransferase knock out (GalT KO) pig, to minimize immune rejection and analyze body immune system for future xenotransplantation studies. Isolation of primary EC from aorta were performed by incubation with dispase for 8-10 min at 37°C. Primary EC were cultured in EC growth medium on different extra cellular matrix (ECM), either collagen or gelation. Primary EC exhibits morphological characteristics and showed positive expressions of EC specific marker proteins i.e. PECAM1, KDR and VWF despite of their ECM surface; however, on collagen based surface they showed increase in mRNA level analyzed by qPCR. Primary EC cultured on collagen were sorted by flow cytometer using KDR marker and cultured as KDR positive cells and KDR negative cells, respectively. KDR positive cells showed dramatically increased in PECAM1 and VWF level as compared to KDR negative cells. Based on the above results, primary EC derived from GalT KO are successfully isolated and survived continuously in culture without becoming overgrown by fibroblast. Therefore, they can be utilize for xeno organ transfer, tissue engineering, and immune rejection study in future.

hPDX1 유전자의 삽입에 의한 직접 췌도세포 분화

옥선아,오건봉,황성수,김영임,권대진,임기순 사단법인 한국동물생명공학회 2015 한국동물생명공학회지 Vol.30 No.3

Diabetes mellitus, the most common metabolic disorder, is divided into two types: type 1 and type 2. The essential treatment of type 1 diabetes, caused by immune-mediated destruction of β-cells, is transplantation of the pancreas; however, this treatment is limited by issues such as the lack of donors for islet transplantation and immune rejection. As an alternative approach, stem cell therapy has been used as a new tool. The present study revealed that bone marrowderived mesenchymal stromal cells (BM-MSCs) could be transdifferentiated into pancreatic cells by the insertion of a key gene for embryonic development of the pancreas, the pancreatic and duodenal homeobox factor 1 (PDX1). To avoid immune rejection associated with xenotransplantation and to develop a new cell-based treatment, BM-MSCs from α-1,3-galactosyltransferase knockout (GalT KO) pigs were used as the source of the cells. Transfection of the EGFP-hPDX1 gene into GalT KO pig-derived BM-MSCs was performed by electroporation. Cells were evaluated for hPDX1 expression by immunofluorescence and RT-PCR. Transdifferentiation into pancreatic cells was confirmed by morphological transformation, immunofluorescence, and endogenous pPDX1 gene expression. At 3∼4 weeks after transduction, cell morphology changed from spindle-like shape to round shape, similar to that observed in cuboidal epithelium expressing EGFP. Results of RT-PCR confirmed the expression of both exogenous hPDX1 and endogenous pPDX1. Therefore, GalT KO pig-derived BM-MSCs transdifferentiated into pancreatic cells by transfection of hPDX1. The present results are indicative of the therapeutic potential of PDX1-expressing GalT KO pig-derived BM-MSCs in β-cell replacement. This potential needs to be explored further by using in vivo studies to confirm these findings.

家庭管理學科 構成에 關한 檢討에 대한 討論

玉先花(옥선화) 가정과삶의질학회 1990 가정과삶의질연구 Vol.8 No.2