미슬토 추출물(Mistletoe Extract)이 위암환자의 수술 후 면역기능에 미치는 효과

양성우,신동규,김일명,윤성민,이용직,허수학,김태희,Yang, Sung-Woo,Shin, Dong-Gue,Kim, Il-Myung,Yoon, Seong-Min,Lee, Yong-Jik,Heo, Su-Hak,Kim, Tae-Hee 대한위암학회 2007 대한위암학회지 Vol.7 No.3

목적: 미슬토 추출물은 서양에서 오래전부터 사용되어진 면역치료 물질로 위암에 대해서는 연구가 부족한 실정이다. 본 연구에서 수술 받은 위암환자를 대상으로 항암제와 미슬토 추출물의 병합투여가 면역기능에 미치는 효과를 파악하기 위해 시행되었다. 대상 및 방법: 원발성 위암으로 진단받고 근치적 수술을 받은 10명의 환자를 선정하여 항암화학요법과 병행하여 수술 후 7일째부터 미슬토 추출물(ABNOVA viscum-Q)을 주 3회, 총 16주간 피하주사 하였다. 면역기능에 미치는 효과는 환자의 말초혈액에서 백혈구수와 그 분획, 백혈구에 대한 총림프구의 분율 그리고 시토카인들(Interleukin-$1{\beta}$, Interleukin-2, Interleukin-6, Interferon-$\gamma$, Tumor necrosis factor-$\alpha$)의 변화를 분석하였다. 상기 항목들은 수술 전과 수술 후 8주, 16주 총 3회 측정되었다. 결과: 남녀비는 9 : 1이며, 평균연령은 55.9세(range $33{\sim}74$세)였다. 병기는 stage Ib가 4명, II가 6명이었다. 백혈구수와 총호중구수는 기저치에 비해 치료 후 8주와 16주에 유의한 감소를 보였다. 총호산구수는 기저치에 비해 8주와 16주에 증가하는 경향을 보였지만 통계적 유의성은 없었다(P=0.15). 총림프구의 수치는 치료 전후 유의한 감소를 보이지만 백혈구 수에 대한 총 림프구의 분율은 유의하지는 않지만(P=0.91) 오히려 증가하고 있다. 각각의 시토카인들은 치료전후 큰 변화를 보이지 않았다. 결론: 치료 전후 의미있는 면역반응의 증가는 관찰하기 어려웠다. 이것은 미슬토 추출물과 항암제의 병용투여에 의한 면역활성과 억제의 상쇄반응으로 생각된다. 총호산구수가 증가하였고, 백혈구에 대한 림프구의 분율이 감소하지 않고 증가하는 양상을 보인 것은 미슬토 추출물에 의해 유발된 면역반응으로 생각된다. Purpose: Mistletoe (Viscum album L.) extract is one of the most widely used agents in alternative cancer therapeutic regimens in Europe. This study was conducted to determine the effect of mistletoe extract on immune function in gastric cancer patients. Materials and Methods: Ten patients that had undergone a curative gastrectomy were enrolled in the prospective study. ABNOBAviscum $Q^{(R)}$ was injected subcutaneously three times a week from postoperative-day 7 to week 16 with an increasing dose. All of the patients simultaneously received chemotheraphy with mitomycin, oral 5-FU and a cisplatin regimen. The WBC count, differential count, lymphocyte/WBC ratio and the level of cytokines (IL-$1{\beta}$, IL-2, IL-6, IFN-$\gamma$, TNF-$\alpha$) were checked in the peripheral blood preoperatively, at postoperative week 8 and at postoperative week 16. Results: The WBC and neutrophil counts significantly decreased after treatment on week 8 and week 16 (P=0.001), but the total eosinophil count was slightly increased (P=0.15). The total lymphocyte count also decreased during treatment but the lymphocyte/WBC ratio was slightly increased without statistical significance (P=0.91). The cytokine levels did not significantly change during treatment. Conclusion: It is somewhat difficult to determine the direct effect of mistletoe therapy on immune function as the effect may be compromised by the concurrent chemotherapy. It can be assumed that the slightly increased lymphocyte/WBC ratio and eosinophil count may be a result of the immunomodulatory effect of the mistletoe extract.

두충(杜?)이 난소적출(卵巢摘出) 흰쥐의 골조직(骨組織) 형태(形態)와 골대사(骨代謝)에 미치는 영향(影響)

양성우,조정훈,장준복,이경섭,Yang, Sung-Woo,Cho, Jung-Hoon,Jang, Jun-Bock,Lee, Kyung-Sub 대한한방부인과학회 2005 大韓韓方婦人科學會誌 Vol.18 No.1

Objective : This study was undertaken to investigate the effects of Eucommiae Cortex on the osteoporosis in ovariectomized rats. Materials and Methods : We used Sprague-Dawley female rats in 8-week-old. They were divided into three groups. Sample group was ovariectomized and administered with 10 mg/100 g/day Eucommiae Cortex extract solution for 10 weeks. Control group was ovariectomized and sham group was conducted by sham's operation. And control group and sham group were administered with normal saline as the same way. We measured rats's body and uterus weight and also measured the serum levels of Ca, phosphorus and ALP. We stained the specimens of rat's tibial bones with Goldner's modified Masson's Trichrome and then examined bone histomorphometry with Bioquant computer program of image analysis system. We measured the thickness of osteoid and callus as static parameters and measured bone volume and mineral apposition rate as dynamic parameters. We observed the expressions of RANKL and OPG mRNA of the tibial bone by RT-PCR. Results : The body weight was significantly (p<0.05) increased in control and sample groups compared with sham group, respectively. The uterus weight was significantly (p<0.05) decreased in control and sample group compared with sham group. In the change of Ca, phosphorus and ALP there were no significant changes among three groups. There were no significant changes of trabecula cortical and osteoid bones' thickness and volume. But trabecula mineral apposition rate (MAR) was significantly (p<0.05) increased in sham and sample group compared with control group. In the expression of RANKL mRNA, sample group was decreased compared with control group, and in that of OPG mRNA, sample group was increased compared with control group. Conclusion : This study shows that Eucommiae Cortex has the beneficial effects on bone histomorphometry and metabolism in the ovariectomized rats. We suggest that Eucommiae Cortex be useful for the treatment of osteoporosis.

Paclitaxel과 Carboplatin 항암 화학 요법 후에 발생한 위막 대장염

양성우 ( Sung Woo Yang ),문원 ( Won Moon ) 대한소화기학회 2009 대한소화기학회지 Vol.54 No.5

Antibiotics-associated pseudomembranous colitis is well documented and caused by abnormal overgrowth of toxin producing Clostridium difficile colonizing the large bowel of patients undergoing antibiotic therapy. Administration of chemotherapeutic agents is frequently complicated by diarrhea and enterocolitis. However, pseudomembranous colitis related to chemotherapeutic agent usage is very rare. We experienced a 67 old-years male patient diagnosed of non-small cell lung carcinoma who complained of watery diarrhea and abdominal pain after treated with paclitaxel and carboplatin. Sigmoidoscopic examination revealed diffusely scattered, whitish to yellowish pseudomembrane with background edematous hyperemic mucosa from sigmoid colon to rectum. Histopathologic findings were consistent with pseudomembranous colitis as typical volcano-like exudate. The symptoms improved after stopping chemotherapy and treatment with metronidazole. In patients with persistent diarrhea and abdominal pain after receiving chemotherapy agents, although rare, pseudomembranous colitis should be considered as a differential diagnosis. (Korean J Gastroenterol 2009;54:328-332)

장기간 게이트볼 운동이 고령자의 혈관탄성에 미치는 영향

양성우(Sung Woo Yang),김정태(Jung Tae Kim),나재철(Jae Chul Na) 한국사회체육학회 2006 한국사회체육학회지 Vol.0 No.28

서킷에서의 데이터 로깅 시스템 실제 활용 사례

양성우(Sung-woo Yang),장성국(Sungkuk Jang) 한국자동차공학회 2011 오토저널 Vol.33 No.2

복분자(Rubus coreanus Miquel)로부터 Helicobacter pylori Urease Inhibitor의 분리 및 특성

양성우(Sung Woo Yang),호진녕(Jin Nyoung Ho),이유현(Yoo Hyun Lee),신동훈(Dong Hoon Shin),홍범식(Bum Shik Hong),조홍연(Hong Yon Cho) 한국식품영양과학회 2004 한국식품영양과학회지 Vol.33 No.5

위염과 위궤양의 일차적 발병인자로 알려진 Helicobacter pylori의 생육을 억제하고 urease 산물인 암모니아의 축적에 의한 위점막 손상을 완화시킬 목적으로 복분자로부터 H.pylori urease 저해물질을 분리정제하고 소재화와 관련된 일부 성질을 규명하였다. 양념채소류, 차류, 죽류, 건강채소류 등의 식용식물, 약용식물, 허브 및 해조류, 총 173종으로부터 극성도에 따라 계통추출한 수용성인 냉수추출물(Fr. 1), methanol 추출물(Fr. 4), 열수추출물(Fr. 5) 519점을 대상으로 H.pylori urease 저해활성을 검색하였다. 1차 및 2차 저해활성 검색 결과 복분자의 70% acetone추출물이 약 24%의 가장 높은 저해활성을 나타내었으며, 이 추출물을 ethyl acetate와 butanol을 사용하여 ethyl acetate/DW layer(RCE/RCW1)와 butanol/DW layer(RCB/RCW2)로 순차 분획한 후 활성획분인 RCW2 내의 활성본체를 확인할 목적으로 periodate oxidation과 pronase digestion을 실시한 결과 펩타이드 또는 단백질성 물질로 판명되었다. 저해활성물질은 DEAE-Toyopearl 650C, Butyl-Toyopearl 650M 및 Sephadex LH-20 순의 column chromatography에 의해 분리정제되었다. 분리urease 저해물질, RCW2-Ⅲcα는 HPLC의 gel permeation chromatography에 의해 비교적 순도 높은 분자량 약 13 kDa의 단일 물질임이 확인되었다. 저해활성물질은 열에 안정성을 보이는 내열성 단백질임을 알 수 있었고 위내 단백분해효소인 pepsin에도 가수분해 저항성을 나타냄으로써 기능성식품의 소재로 높은 소재화 적성을 보였다. A Helicobacter pylori urease inhibitor from Rubus coreanus Miquel has been isolated and partially characterized for aiming to prevent H. pylori growth and decrease harmful accumulation of ammonia in human gastric mucosa. We screened urease inhibitory activities in 519 extracts library prepared by solvent extraction from 173 kinds of edible plants, medicinal herbs, herbs and seaweeds using a colorimetric urease assay system. As results of primary and secondary screening, 70% acetone extract of Rubus coreanus Miquel was selected as potent candidate, showing about 24% inhibitory activity. The acetone extract was sequentially partitioned into RCE/RCW1 and RCB/RCW2 layers with ethyl acetate and butanol. The major active component in RCW2, water layer from butanol fractionation was revealed to be peptidic or proteinous substance by inhibitory activity determination after pronase digestion and periodate oxidation. RCW2-Ⅲc α was isolated by sequential column chromatography on DEAE-Toyopearl 650C, Butyl-Toyopearl 650M and Sephadex LH-20. The isolated urease inhibitor, RCW2-Ⅲcα, was highly pure proteinous substance with molecular weight of 13 kDa by high-performance gel permeation liquid chromatography. RCW2-Ⅲcα has about 5 times higher inhibitory activity than 70% acetone extract, showing high stability against heat treatment and peptic digestion.

비소세포 폐암환자의 객담 내 ERCC1 발현정도와 cisplatin 복합화학요법 후 치료반응

양성우 ( Sung Woo Yang ),최평락 ( Pyoung Rak Choi ),류홍준 ( Hong Jun You ),김진구 ( Jin Gu Kim ),옥철호 ( Chul Ho Oak ),장태원 ( Tae Won Jang ),정만홍 ( Maan Hong Jung ) 대한결핵 및 호흡기학회 2006 Tuberculosis and Respiratory Diseases Vol.60 No.2

공공 흡연장에서의 흡연 중 침 뱉기가 흡연장 주변 바닥의 미생물 오염도 변화와 미생물 확산에 미치는 영향

양성우(Sung Woo Yang),여승현(Seung Hyeon Reo),김승준(Seung Jun Kim),조정욱(Jeong Wook Jo),최용근(Yong-Keun Choi),송학진(Hak Jin Song),오경빈(Kyung Bin Oh),이주연(Ju Yeon Lee),정윤석(Yunseok Jung),김형주(Hyung Joo Kim) 대한환경공학회 2021 대한환경공학회지 Vol.43 No.11

목적 : 본 연구의 목적은 공공 흡연장에서 흡연 중 바닥에 직접 침을 뱉는 행위가 공중위생에 미치는 영향을 조사하는 것이다. 이 연구를 통하여 향후의 공중위생과 환경을 위해 흡연장에서의 세균 농도와 세균의 확산을 평가하였다. 방법 : 흡연 구역에서 침 뱉는 횟수와 침 뱉는 위치를 관찰자들이 시각적으로 측정하였다. 미생물 분석을 위해 거름종이(5 × 5 cm)를 일정한 시간 동안 흡연 구역의 바닥 표면에 부착하여 샘플을 채취하였다. 샘플을 채취한 거름종이를 이용하여 ATP bioluminescence (RLU), Colony forming unit (CFU)를 측정하였다. 흡연장에서 침 뱉는 행위를 통한 미생물의 확산을 확인하기 위해 흡연장에서 침이 존재하는 거름종이를 선정하여 형광분말(0.01 g/㎠)을 거름종이에 도포하였다. 4시간 후에 흡연장 주변의 거름종이를 수거하여 거름종이의 형광량(RFU, Relative Fluorescence Unit)을 측정하였다. 결과 및 토의 : 흡연장 바닥의 침 뱉는 행위와 그에 따른 흡연장 바닥의 미생물 활성이 명확한 관계가 있다는 결과를 보여주었다. 시료를 채취한 A 구역의 경우, 점심시간에 가장 많은 침 뱉는 횟수(102 ± 16회)가 흡연통 주변에서 관찰되었다. 이때 흡연을 하면서 침을 뱉는 흡연통 근처의 15개 구역에서 채취한 시료의 평균 RLU는 5,139 ± 1,267로 측정되었다. 또한 흡연장 주변 RLU 측정 시, 흡연통으로부터 11 m, 17 m 떨어진 곳에서 가장 낮은 값이 확인되었으며(1,329 ± 148 RLU, 1,204 ± 203 RLU), 이 장소는 흡연 및 침 뱉기가 발생하지 않은 곳으로 확인되었다. 시료를 채취한 B 구역의 경우는 저녁 시간에 가장 많은 침 뱉는 횟수(45 ± 6회)가 관찰되었다. B 구역의 흡연을 하면서 침을 뱉는 흡연통 근처 15개의 장소 RLU는 5,274 ± 1,297 RLU로 측정되었으며, 흡연통에서 11 m, 18 m 떨어진 곳에서 가장 낮은 RLU (1,181 ± 243 RLU, 1,148 ± 168 RLU)가 측정되었다. 이 구역 또한 흡연 및 침 또한 뱉지 않은 구역으로 확인되었다. 이상의 결과는 침 뱉는 행위 및 바닥의 침이 흡연장 바닥의 미생물 오염도에 영향을 준다는 것을 보여주고 있다. 형광분말 확산 분석 결과는 흡연장 표면에서 침의 존재가 흡연장으로부터 다른 지역으로 미생물의 확산을 유발할 수 있다는 것을 나타내고 있다. 결론 : 침을 뱉는 행위는 흡연장에 있는 흡연자들과 비흡연자들이 미생물에 감염될 수 있는 위험을 유발할 수 있다. 따라서 공중위생을 안전하게 유지하기 위해서는, 흡연 중 침 뱉기의 위험성에 대한 설명과 주의가 필요하다고 판단된다. Objectives : The purpose of this study was to investigate the effect of spitting behavior on microbial public health in smoking areas. Through this investigation, bacterial concentration and spread of bacteria from the smoking areas were evaluated for the prospective public health and environment. Methods : The number of spitting and the spitting location in the smoking areas were measured visually by observers. For the microbial analysis, filter papers (5 × 5 cm) as sample collectors had been attached to the surface of the smoking areas for a given time. Using the collected filter paper, ATP bioluminescence measurement (RLU), colony forming unit (CFU) were measured. For the estimation of spitting behaviors in the smoking area on the spread microorganisms, saliva containing filters were selected from the areas, and fluorescent powder (0.01 g/㎠) was added to the papers. After 4 hours, the papers around the smoking areas were collected and the fluorescence activity was measured. Results and Discussion : The results showed that there was a clear relationship between the spitting behavior and the microbial activity in the smoking areas. In the case of sampling area A, the highest number of spitting was observed at the lunch hour (102 ± 16 times), and the RLU from the sample collected near the smoking area showed about 5,139 ± 1,267 RLU. When the RLU around the smoking area was measured, the lowest values were found at distance of 11 and 17 m from the cigarette bin (1,329 ± 148 and 1,204 ± 203 RLU), and it was confirmed that smoking and spitting did not occur at this sampling points. In the case of sampling area B, the highest number of spitting (45 ± 6 times) was observed at evening hour, and the RLU from the sample collected near the smoking area showed about 5,274 ± 1,297 RLU. The lowest value was in the sampling area B shown at 11 and 18 m (1,181 ± 243 RLU and 1,148 ± 168 RLU) from the cigarette bin, and it was also confirmed that smoking and spitting did not occur at this sampling points. The results indicate that the spitting during the smoking induced the increases of the microbial contamination of the public environment. The fluorescence powder diffusion analysis indicated that the saliva on the ground surface would enhance the spread of the microorganisms from the smoking area. Conclusions : Spitting in smoking areas can pose a high risk of microbial infection for smokers and non-smokers in smoking areas. Thus extensive and systematic attention related to the smoking manner must be paid to protect our public health.

운동과 2,3-DPG(diphosphoglycerate)와의 관계

강성훈(Kang Sung-Hwun),양성우(Yang Sung-woo),이재규(Lee Jae-Gue),박일봉(Park Il-Bong),차유림(Cha Yu-Rim),오경식(Oh Kyung-Sik),여남회(Yeo Nam-Hwoeh) 동아대학교 스포츠과학연구소 2005 스포츠科學硏究論文集 Vol.23 No.-

  2,3-DPG is synthesized from glycolytic intermediates by a pathway known as Rapoport-Luebering shunt. The production of 2,3-DPG depends on the relative amounts of its precursor, 1,3-DPG going into Rapoport-Leubering pathway and into the ATP-forming glycolytic pathway. Its concentration also depends on the rate of hydrolysis of 2,3-DPG The most important function of 2,3-DPG is its effect on the oxygen affinity of Hb. Normal Hb in deoxygenated state can bind 2,3-DPG in a molar ration of 1:1 which results in reduced oxygen affinity of Hb and enhanced oxygen delivery to tissues. In oxygenated state the ability of Hb to bind 2,3-DPG is decreased and most DPG is in free state. As the result the affinity of Hb to oxygen is increased and it binds more of oxygen. Changes in 2,3-DPG levels have important role in adaptation to hypoxia. In anemia and certain hypoximic conditions, 2,3-DPG level in erythrocytes increase and oxygen affinity of Hb is reduced resulting in improved delivery of oxygen to tissues. This study was invested relative in exercise and 2,3-DPG we had a conclusion as follows; 2,3-DPG was increased in anemia, congestive heart failure, heart disease, hyperthyroidism but it was decreased in diabetic. Hypoxia was decreased and extremely exercise was increased.

운동과 EPO(Erythropoietin)와의 관계

강성훈(Kang Sung-Hwun),이재규(Lee Jae-Gue),양성우(Yang Sung-woo),박일봉(Park Il-Bong),차유림(Cha Yu-Rim),오경식(Oh Kyung-Sik),여남회(Yeo Nam-Hwoeh) 동아대학교 스포츠과학연구소 2005 스포츠科學硏究論文集 Vol.23 No.-

  Erythropoietin is produced mainly in the kidney and regulates erythrocyte production. It binds to a cell surface receptor and leads to intracellular activation of several kinase pathways. EPO is a hypoxia-induced hormone that is essential for normal erythropoiesis. The production of recombinant human erythropoietin has revolutionized the treatment of anemia associated with chronic renal failure and chemotherapy. EPO has similar structure and signaling mechanisms to the family of type I cytokines and is markedly induced by hypoxia. EPO is synthesize by peritubular cells in the cortex-medullary border of the kidney and in the liver during fetal and neonatal development. A variety of other tissues have been reported to express erythropoietin including bore marrow macrophages, trophoblasts, breast glands, and astrocytes.<BR>  Therefore, This study was invested relative in exercise and EPO, we had a conclusion as follows; 1) EPO is useful on polycythemia in test. 2) In patients with chronic renal insufficiency, r-HuEPO has also been shown to improve exercise capacity. 3) EPO has similar structure type I cytokines and induced by hypoxia.