식용 식물자원으로부터 활성물질의 탐색-I. -고구마(Ipomoea batatas Lam.) 괴근 주요성분의 분리-

안은미,방면호,김해영,백남인,Ahn, Eun-Mi,Bang, Myun-Ho,Kim, Hae-Yeong,Baek, Nam-In 한국응용생명화학회 1997 Applied Biological Chemistry (Appl Biol Chem) Vol.44 No.4

고구마 괴근을 MeOH 수용액으로 추출하고, 얻어진 추출물을 EtOAc, n-BuOH 및 물로 용매분획하였다. EtOAc 분획에 대하여 column chromatography를 반복하여 4종의 화합물을 분리하였다. 각 화합물의 화학구조를 NMR, MS 등 스펙트럼 데이터의 해석 및 가수분해, 메칠화 및 아세칠화반응을 적용하여, 수지 배당체, simonin I, ${\beta}-sitosterol$ 및 2종의 불포화지방산 화합물로 구명하였다. The MeOH extracts obtained from the tuber of Ipomoea batatas Lam. were solvent-fractionated with EtOAc, n-BuOH, and $H_2O$, respectively. From EtOAc fraction four different compounds were isolated through the repeated silica gel column chromatographies. From not only the results of NMR and MS data but also the adaptation of hydrolysis, methylation, and acetylation, the chemical structures of compounds were elucidated as resin glycoside, simonin I, ${\beta}-sitosterol$, and two kinds of unsaturated fatty acids.

지금은 최저임금 투쟁의 깃발을 올려야 할 때

안은미 한국노동조합총연맹 2012 월간 한국노총 Vol.483 No.-

최저임금 6.1% 인상, 그러나 끝나지 않은 싸움

안은미 한국노동조합총연맹 2012 월간 한국노총 Vol.484 No.-

국민연금 가입자가 기초연금법을 반대하는 이유

안은미 한국노동조합총연맹 2013 월간 한국노총 Vol.498 No.-

노동자 · 청년의 고용연대 첫발을 내딛다

안은미 한국노동조합총연맹 2010 월간 한국노총 Vol.2010 No.12

최저임금위원회 파행 원인은 정부에 있다!

안은미 한국노동조합총연맹 2012 월간 한국노총 Vol.482 No.-

The ameliorative effect of Protaetia brevitarsis Larvae in HFD-induced obese mice

안은미,명노일,정현아,김수진 한국식품과학회 2019 Food Science and Biotechnology Vol.28 No.4

Protaetia brevitarsis Lewis (P. brevitarsis) larvae,edible insect, traditionally is consumed for varioushealth benefits. However, little information is availablewith respect to its direct anti-obesity effects. Thus, thepresent study was designed to investigate the regulatoryeffect of P. brevitarsis against high-fat diet (HFD)-inducedobese mice. HFD-fed mice showed an increase in the bodyweight and serum levels of total cholesterol as well as lowdensitylipoprotein-cholesterol, and triglycerides. Theadministration of P. brevitarsis to obese mice induced areduction in their body weight, lipid accumulation in liverand serum lipid parameter compared with the HFD fedmice. P. brevitarsis also inhibited the expression of obesity-related genes such as CCAAT/enhancer-binding proteinalpha and fatty acid synthesis in 3T3-L1 cells. Moreover, oleic acid was identified as predominant fattyacid of P. brevitarsis by gas chromatography analysis. Conclusively, these findings suggested that P. brevitarsismay help to prevent obesity and obesity-related metabolicdiseases.

수종 생약재의 GABA 대사 관련 효소의 활성에 미치는 영향

안은미,한재택,박진규,조성우,전성규,반재훈,선현정,최수영,백남인,Ahn, Eun-Mi,Han, Jae-Taek,Park, Jin-Kyu,Cho, Sung-Woo,Jeon, Seong-Gyu,Bahn, Jae-Hoon,Sun, Hyun-Jung,Choi, Soo-Young,Baek, Nam-In 한국생약학회 2000 생약학회지 Vol.31 No.1

The effect of seventy kinds of medicinal plants on the activities of GABA-metabolizing enzymes as glutamate dehydrogenase I (GDH I), glutamate dehydrogenase II (GDH II), GABA transaminase (GABA-T), succinic semialdehyde dehydrogenase (SSADH) and succinic semialdehyde reductase (SSAR) were estimated. The following plants extracts from Acori graminei Rhizoma, Longnae Arillus, Gastrodiae Herba, Lycii Fructus, Ligusticum officinale, Ferula assafoetida, Corydalis Tuber, Eucommiae Cortex, Zizyphi spinosi Semen activated the activity of GDH I to more than 35%, and the following ones from Visci Ramulus, Ligusticum officinale, Myristicae Semen, Ferulae Resina, Scolopendrae Corpus, Corydalis Tuber, Eucommiae Cortex, Zizyphi spinosi Semen did that of GDH II. The plant extracts from Cynanchi Radix, Astragali Semen, Angelicae dahuricae Radix, Biotae orientalis Folium, Uncariae Ramulus et Uncus, Polygalae Radix, Cynomorii Herba inhibited that of GABA-T to 35% and over, and the following ones from Hyoscyamus niger, Cynanchi Radix, Acori graminei, Caesalpiniae Lignum, Cannabis Semen, Sedum aizoon, Sedum kamtschaticum, Schisandrae Fructus, Lilii Bulbus, Biotae orientalis Folium, Uncariae Ramulus et Uncus, Myristicae Semen, Akebiae Fructus, Cynomorii Herba, Buddleiae Flos, Mucunae Caulis, Zizyphi Fructus, Paeoniae Radix rubra did that of SSADH to 70% and over; the following ones from, Caesalpiniae Lignum, Sedum kamtschaticum, Schisandrae Fructus, Astragali Semen, Angelicae dahuricae Radix, Dioscorea nipponica, Myristicae Semen, Akebiae Fructus, Cynomorii Herba, Scutellariae Radix did that of SSAR.

Analytical Methods for the Determination of Pesticide Residues Using Gas Chromatograghy with Nitrogen-phosphorus Detector

안은미,신한승 한국식품과학회 2011 Food Science and Biotechnology Vol.20 No.2

The analysis methods for 3 pesticides (ametryn,cyanophos, and fonofos) were developed and validated. One 1,3,5-triazine herbicide (ametryn), and 2 organophosphorus insecticides (cyanophos and fonofos) were tested. Three kinds of residual pesticides were classified into 2 groups according to the chemical structure and separation method. Solid-phase extraction (SPE) cartridge was used for sample purification and isolation of analytes. Final solution was analyzed with GC with nitrogen-phosphorus detection (NPD). Confirmation analysis of pesticides was carried out by GC-MS in the selected ion monitoring (SIM) mode. Correlation coefficient was ranged from 0.999 to 1.0,depending on sample analytes. Rice, apple, and soybean samples were selected for recovery experiment. Overall recovery rates from blank samples spiked at 3 fortification levels ranged from 76.29 to 107.4% with relative standard deviations lower than 20.2%. The limit of quantification was <3-5 μg/L depending on the analytes, and the reporting level of the method, defined as lower than the maximum residue levels established by Korea Food &Drug Administration (KFDA).

북한 이탈주민의 증상표현과 질병행태: 효과적인 치료적 관계를 형성하기 위하여

안은미,송종임,강현석,박정준,유상호,허봉렬 대한가정의학회 2007 Korean Journal of Family Medicine Vol.28 No.5

Background: The North Korean defectors are suffering from multiple psychologic and physical health problems. However, because of their emotional maladaptation, noncooperation and suspiciousness and distrust toward others, it is difficult for South Korean doctors to build effective therapeutic relationships with them. Therefore, we made efforts to clarify the characteristics of North Korean defectors in symptom expressions and illness behaviors which would help South Korean doctors to gain rapport. Methods: We performed qualitative study with focus group interview. Three focus groups were composed of 14 North Korean defectors and group interviews were performed twice for each group. The contents of each interview were analyzed and conclusions were drawn by extracting and arranging significant findings. Results: The North Korean defectors showed psychologic symptoms such as anxiety, depression and various somatic symptoms. Among them, ‘Laeng-Dol (‘cold mass’) and ‘Jeog’ mean subjective mass-feeling, such as globus sensation. And ‘Tag-Gi-Byeong (‘chicken heart disease’)’ means recurrent chest pain. These expressions reflect their anxious, depressive, and somatizing tendency. Among illness concept, ‘Laeng-Byeong’ (‘cold related disease’) means that they consider the cold weather to be the cause of various somatic symptoms. Characteristic illness behaviors include continuous complaining of recurrent symptoms, distrusting medical staffs and institutions, self-diagnosis, self- prescription, and reliance upon folk remedies. These illness behaviors were precipitated by the current breakdown of health-care system and economic crisis of the North Korea. Conclusion: South Korean doctors should be able to understand these characteristics of the North Korean defectors in order to build effective therapeutic relationship with them. (J Korean Acad Fam Med 2007;28:352-358) 연구 배경: 현재 남한 내에는 5,000명 이상의 북한이탈주민들이 생활하고 있으며, 국내 입국 탈북자의 숫자도 지속적으로 높은 증가 추세를 보이고 있다. 북한이탈주민들의 심리 및 행동 특성에 대한 선행 연구에 의하면, 이들은 심한 불안, 불신, 심리적 부적응을 보이며, 비타협성, 공격성, 의존성, 관계회피 등의 특성을 나타낸다고 보고하고 있어 환자-의사 간의 원만한 치료적 관계 형성이 어려울 수 있음을 시사하고 있다. 이에 본 연구에서는 효과적인 치료적 관계를 형성하기 위하여 남한의사가 알아두어야 할 북한이탈주민들의 증상 표현과 질병 행태의 특성을 이해해 보고자 한다. 방법: 포커스 그룹 인터뷰(focus group interview)를 통한 질적인 연구를 시행하였다. 북한이탈 여성 14명을 3개의 그룹으로 나누어 본인과 가족의 질병 경험에 대한 인터뷰를 실시한 후 이를 바탕으로 의미 있는 자료들을 축약하여 배치하고 결론을 도출하였다. 결과: 증상표현 중에는 불안, 불면, 우울 등의 정신적 증상표현과, 만성적인 소화기, 비뇨생식기 증상 등의 신체적 증상표현이 반복적으로 관찰되었다. 이 중 ‘랭돌’, ‘닭기병’ 등의 표현에는 공통적으로 불안, 우울, 신체화 경향이 반영되어 있었다. ‘심장압박, 심장경련, 심장신경증’은 불안과 관련된 증상을 심장, 신경과 연관시킨 질병개념이었으며, ‘냉병’은 추운 기후를 다양한 질병의 원인으로 생각하는 개념이었다. 질병행태에서는 증상에 대한 집착, 의료기관에 대한 불신, 자가 진단과 처방, 민간요법에 대한 높은 의존도 등이 두드러졌으며, 그 바탕에는 북한의 사회 환경, 특히 경제난으로 인한 의료체계의 붕괴가 자리잡고 있었다. 결론: 남한 의사들은 북한이탈주민들과 효과적인 치료적 관계를 형성하기 위해 이들의 증상표현과 질병행태의 특징을 이해하고 적절하게 대처할 수 있어야 할 것이다.