http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
안경익,차준영,Lee Jeong Won,Park Gyeongran,신경임,Song Shi-Jian,Ryu Gyeongryul,황인환,김민갑,김외연 한국식물학회 2022 Journal of Plant Biology Vol.65 No.1
Viral diseases are extremely widespread infections that change constantly through mutations. To produce vaccines against viral diseases, transient expression systems are employed, and Nicotiana benthamiana (tobacco) plants are a rapidly expanding platform. In this study, we developed a recombinant protein vaccine targeting the major capsid protein (MCP) of iridovirus fused with the lysine motif (LysM) and coiled-coil domain of coronin 1 (ccCor1) for surface display using Lactococcus lactis. The protein was abundantly produced in N. benthamiana in its N-glycosylated form. Total soluble proteins isolated from infiltrated N. benthamiana leaves were treated sequentially with increasing ammonium sulfate solution, and recombinant MCP mainly precipitated at 40–60%. Additionally, affinity chromatography using Ni–NTA resin was applied for further purification. Native structure analysis using size exclusion chromatography showed that recombinant MCP existed in a large oligomeric form. A minimum OD600 value of 0.4 trichloroacetic acid (TCA)-treated L. lactis was required for efficient recombinant MCP display. Immunogenicity of recombinant MCP was assessed in a mouse model through enzyme-linked immunosorbent assay (ELISA) with serum-injected recombinant MCP-displaying L. lactis. In summary, we developed a plant-based recombinant vaccine production system combined with surface display on L. lactis.
제품모델 표준 기반 원자력발전소 통합정보관리시스템 구현 사례
안경익 (사)한국CDE학회 2014 한국 CAD/CAM 학회 학술발표회 논문집 Vol.2014 No.8
The initial purpose of this study is focused on how to integrate NPP life cycle information which is generated from heterogeneous information system. To fulfill this purpose, product data technology is involved in this study. ISO 10303 is an ISO standard for the computer-interpretable representation and exchange of product manufacturing information. The international standard"s objective is to provide a mechanism that is capable of describing product data throughout the life cycle of a product, independent from any particular system. Also systems engineering’s V-Model approach is involved to solve the traceability between NPP life cycle stage and its information and the model can also provide how to manage configuration information through their life cycle. Author has developed an web based information management system which is called PLIMS ? Plant Life cycle Information Management System, and the system provides capabilities to manage MEL, design requirements, engineering data, and plant configuration using the V-model approach. In addition, this study conducted a research and development project and tests the prototype system using legacy plant information to verify its feasibility. It has been demonstrated that this approach can provide not only provide an explicit CM and control on the information, but can also provide a more efficient method to integrate, manage and visualize complex plant information.