역상 액체 크로마토그래피에서 반복회귀법에 의한 페놀류의 분리 최적화

김인환,신찬성,김상태,이만호 대구대학교 기초과학연구소 1995 基礎科學硏究 Vol.12 No.-

두가지 파라미터를 이용한 반복 회귀 최적화법을 역상 액체 크로마토그래피에 의한 페놀류의 혼합 시료 분리에 적용하였다. 실험에 사용된 파라미터들은 메탄올과 아세토니트릴의 농도이다. 이 방법에 의해 결정된 최적 용매 조성비는 MeOH : ACN : H_(2)O = 40 : 16 : 44 이었다. 이 최적화 방법은 적은 횟수의 실험을 통하여 효과적인 분리 최적화점을 얻을 수 있었다. The iterative regression optimization method using two parameters is applied to the separation of phenols in reversed-phase liquid chromatography. The parameters examined are the concentration of methanol and acetonitrile. The optimum solvent, which gives complete separation of phenols, was -- determined a s follows MeOH : ACN : KO = 40 : 16 : 44 It was obtained an efficient optimization using a small number of initial experiments.

제주도 한라산 1100 고지 대기 에어로졸의 조성 및 특성

김원형,강창희,신찬성,고선영,홍민선 濟州大學校 基礎科學硏究所 2003 基礎科學硏究 Vol.16 No.1

The atmospheric aerosols collected at the 1100 site located in Mt. Halla have been analyzed to investigate their compositions and chemical characteristics. The average concentrations of water-soluble cautions were in the order of NH_(4)^(+)>Ca^(2+)>Na^(+)>K^(+)>Mg^(2+) during the spring, showing high increase of Ca^(2+) concentration with the value of 0.60㎍/㎥, and NH_(4)^(+)>Na^(+)>K^(+)>Ca^(2+)>Mg^(2+) during the other seasons. The average concentrations of anions have shown in the order of SO_(4)^(2-)>NO_(3)^(-)>Cl^(-) for all seasons, and SO_(4)^(2-) and NO_(3)^(-) had higher concentrations in spring with the values of 4.84㎍/㎥ and 1.08㎍/㎥, respectively. From the analytical data of size-segregated particles by cascade impactor sampling, the components of NH_(4)^(+), SO_(4)^(2-), NO_(3)^(-) and K^(+) were distributed mainly in fine particles, but Ca^(2+), Na^(+), Mg^(2+) and Cl^(-) were included mostly in coarse particles. The correlation coefficients of nss-SO_(4)^(2-)/NH_(4)^(+), nss-SO_(4)^(2-)/K^(+) and NO_(3)/nss-Ca^(2+) showed quite high values with 0.871, 0.857 and 0.654, respectively. Based on the study of enrichment factors, it is considered that Na^(+), Mg^(2+), Cl^(-) and Ca^(2+) components were delivered from oceanic and soil sources, but SO_(4)^(2-), NO_(3)^(-) and NH_(4)^(+) might have other source origins. The factor analysis study showed the aerosol at the 1100 site was influenced mainly by anthropogenic factors, followed by oceanic and soil factors.

제주시 지역 빗물 이온의 특성 및 분석 신뢰도 조사 ( Characteristics of Rainwater Ions in Cheju City and Investigation on the Reliability of Their Analytical Data )

홍상범,한지숙,신찬성,김원형,강창희 한국대기환경학회 1997 한국대기환경학회 학술대회논문집 Vol.- No.-

[논문]제주도 한라산 1100 고지 대기 에어로졸의 조성 및 특성

김원형,강창희,신찬성,고선영,홍민선 한국대기환경학회 2003 한국대기환경학회지 Vol.19 No.2

The atmospheric aerosols collected at the 1100 site located in Mt. Halla have been analyzed to investigate their compositions and chemical characteristics. The average concentrations of water soluble cations were in the order of NH₄^+>Ca^2+>Na^+>K^+>Mg^2+ during the spring, showing high increase of Ca^2+ concentration with the value of 0.60㎍/㎥, and NH₄^+>Na^+>K^+>Ca^2+>Mg^2+ during the other seasons. The average concentrations of anions have shown in the order of SO₄^2->NO₃^->Cl^- for all seasons, and SO₄^2- and NO₃^- had higher concentrations in spring with the values of 4.84㎍/㎥ and 1.08㎍/㎥, respectively. From the analytical data of size-segregated particles by cascade impactor sampling, the components of NH₄^+, SO₄^2-, NO₃^- and K^+ were distributed mainly in fine particles, but Ca^2+, Na^+, Mg^2+ and Cl^- were included mostly in coarse particles. The correlation coefficients of nss-SO₄^2-/NH₄^+, nss-SO₄^2-/K^+ and NO₃^-/nss-Ca^2+ showed quite high values with 0.871, 0.857 and 0.654, respectively. Based on the study of enrichment factors. it is considered that Na^+, Mg^2+, Cl^- and Ca^2+ components were delivered from oceanic and soil sources, but SO₄^2-, NO₃^- and NH₄^+ might have other source origins. The factor analysis study showed the aerosol at the 1100 site was influenced mainly by anthropogenic factors, followed by oceanic and soil factors.

PA27)제주지역 강수 성분의 산성화 및 중화 기여도 분석

고희철,강창희,김원형,신찬성,고선영,현진욱 한국대기환경학회 2003 한국대기환경학회 학술대회논문집 Vol.- No.-

광나무 열매 발효 추출물의 항염 및 항산화 활성

김정은,김소희,김미애,고미선,신찬성,이남호 사단법인 대한화장품학회 2023 대한화장품학회지 Vol.49 No.2

In this study, the anti-inflammatory and anti-oxidative activities were compared for the extracts of non-fermented Ligustrum japonicum fruits (LJF) and fermented counterparts. U se of Latilactobacillus curvatus (L. curvatus) and Weissella minor (W. minor), isolated from the Jeju Chromis notata, as fermented strains led to the extracts of LJF-LC and LJF-WM in this experiment. The yield of each fermented extract (LJF-LC and LJF-WM) was 40.5 ~ 46.0%, higher than 29.5% of non-fermented extract (LJF). As a result of an activity experiment using RAW 264.7 macrophages stimulated by lipopolysaccaride (LPS), it was confirmed that LJF-WM, a fermented extract, has an excellent effect of inhibiting NO production in a concentration-dependent manner without cytotoxicity. Upon the screening of DPPH and ABTS+ radical scavenging activities, the fermented LJF-LC and LJF-WM showed comparable to the non-fermented LJF. In the study of cell protection effect using HaCaT keratinocytes damaged by hydrogen peroxide (H2O2), the fermented LJF-WM indicated protective effect against oxidative stress. In addition, quantitative analysis of a major constituent salidroside by HPLC indicated about 15.6 mg/g for the LJF-LC and 13.9 mg/g for the LJF-WM, which were higher than that of non-fermented LJF (12.0 mg/g). Based on these results, it was suggested that the fermented extract from L. japonicum fruits could be used as a natural cosmetics material with anti-inflammatory and anti-oxidative effects. 본 연구에서는 광나무(Ligustrum japonicum) 열매 발효 추출물의 항염 및 항산화 효능을 비발효 추출물과비교 분석하였다. 광나무 열매의 발효는 제주 자리돔(Chromis notata) 내장에서 분리한 Latilactobacillus curvatus (L. curvatus) 및 Weisella minor (W. minor) 균주를 이용하였으며, 각각의 발효 추출물(LJF-LC 및 LJF-WM)의 수율은 40.5 ~ 46.0%로 비발효 추출물(LJF)의 29.5% 보다 높게 나타났다. Lipopolysaccaride (LPS)로 자극된 RAW 264.7 대식세포를 이용한 nitric oxide (NO) 생성 억제 활성 실험 결과, 발효 추출물인LJF-WM이 세포 독성 없이 농도 의존적으로 NO의 생성을 저해시키는 효과가 우수함을 확인하였다. 또한 DPPH 및 ABTS+ 라디칼 소거 활성 실험 결과, 발효 추출물의 라디칼 소거 활성이 비발효 추출물과 유사하게 나타났으며, 발효 추출물 LJF-WM은 과산화수소(H2O2)로 유도된 세포 손상에 대한 세포보호 효과를 보였다. 광나무 열매의주성분인 salidroside의 함량을 HPLC를 이용하여 분석한 결과, 발효 추출물 LJF-LC에서 15.6 mg/g, LJF-WM 에서 13.9 mg/g으로 확인되어 비발효 추출물(12.0 mg/g) 보다 함유량이 높게 분석되었다. 이상의 연구 결과를바탕으로 광나무 열매 발효 추출물은 항염 및 항산화 효과를 갖는 천연 화장품 소재로써 활용 가능할 것이라사료된다.

담팔수의 추출공정에 따른 gallic acid 함량 및 미백, 항균, 항산화 활성 비교

배지영,강윤아,김미애,김소희,신찬성 한국생약학회 2024 생약학회지 Vol.55 No.1

Elaeocarpus sylvestris var. ellipticus (Thunb.) H. Hara (Elaeocarpaceae) is the evergreen tree with shiny and oblanceolate leaves, native to Jeju island. It is known to have antioxidant, antiviral, and antibacterial activities, there are several trials undergoing to develop it as a useful resource. In this study, the skin-related activities and chemical profiles of plant parts of E. sylvestris var. ellipticus as cosmetic ingredients with low toxicity and good efficacy were investigated using sub-critical water extraction method and fermentation method by Latilactobacillus curvatus (fruit fermentation, period 1-3-5 days, respectively). As a result, HPLC profiles showed that each extract showed similar patterns, but the sub-critical water leaf extract of E. sylvestris var. ellipticus (ELS-SWE) has the highest gallic acid content. Anti-oxidant, anti-acne as well as whitening activities were also highest in ELS-SWE, because it contained the highest amount of gallic acid among the other samples tested. From these results, the ELS-SWE can be suggested as a cosmetic whitening material with low-toxicity.