Chitosan-Ascorbate 함유 홍국이 고지방 식이 흰쥐의 혈청지질에 미치는 영향

신진기,이상일,권중호,김순동,Shin Jin-Gi,Lee Sang-Il,Kwon Jung-Ho,Kim Soon-Dong 동아시아식생활학회 2005 동아시아식생활학회지 Vol.15 No.5

Dietary effect of soyfiber beni-koji (SBK) with chitosan-ascorbate (CA) on the level of serum lipids in rats fed a high fat diet was investigated The experimental groups which were divided into high fat diet control group(HC-group), $2\%\;SBK+0.1\%$ CA mixture diet group(CA1-group), $2\%\;SBK+0.15\%$ CA mixture diet group(CA2-group), and $2\%\;SBK+0.2\%$ CA mixture diet group (CA2-group) were fed for 4 weeks. Weight gains in CA2- and CA3-group were $5.3\%\;and\;9.5\%$ lower than that of HC-group, respectively, while there was no significant difference in feed intakes, feed efficiency ratio and organs weight Level of serum triglyceride in $C_3-group\;was\;21\%$ lower than that of HC-group. Level of serum total cholesterol and LDL-cholesterol in CA2- and CA3-group were $22.1\~22.7\%\;and\;58.6\~64.3\%$ lower than those of HC-group, respectively. Atherogenic index decreased with the higher level of CA. Level of lipid peroxide in CA3-group was $24\%$ lower than that of HC-group, while there was no significant difference in GSH(Glutathione-S-transferase) content O type activities of XOD(xanthine oxidase) in the treated groups were lower, especially the activity in CA3-group was $51.6\%$ lower than that of HC-group. Also, O/T ratio of XOD was lower, showing $21.7\~23.5\%$ in treated groups and $34.0\%$ in HC-group(p<0.05). GST activities were 332.52 units in HC-group and $350.28\~355.63$ units in the treated groups, but there were no significant differences among them.

시판 청국장 분말제품의 품질 및 기능성분

이효진(Hyo-Jin Lee),조상아(Sang-A Cho),신진기(Jin-Gi Shin),김정상(Jeong-Sang Kim),정용진(Yong-Jin Jeong),문광덕(Kwong-Duck Moon),권중호(Joong-Ho Kwon) 한국식품영양과학회 2007 한국식품영양과학회지 Vol.36 No.1

청국장 시료의 고품질화 연구의 일환으로 시판 청국장 분말제품(A~E사 시료)에 대한 이화학적, 관능적 품질과 몇가지 기능성분을 분석 비교하였다. 시판 청국장 분말의 일반성분은 수분 6.07~8.54%, 조단백질 15.31~27.07%, 조지방질 20.19~24.75%, 탄수화물 34.84~52.41%, 조회분 3.69~5.26% 범위로 나타났다. 시료의 pH는 5.58~6.11 수준이었으며, 분말의 색상은 시료에 따라 약간의 차이를 보이면서 L값 70.01~77.22, a값 0.91~4.64, b값 23.72~31.00을 나타내었다. 미생물 농도에서 총 세균은 8.16~9.60 log CFU/g, 곰팡이와 효모는 ~4.16 log CFU/g, 대장균군은 1.07~3.88 log CFU/g의 분포를 보였다. 시료의 환원당 함량은 1.89~2.41%, 아미노태질소는 2.83~7.31%이었으며, 유리아미노산 조성은 glutamic acid, valine, leucine, phenylalanine, lysine 등의 순으로 높게 나타났다. 청국장 분말의 총 페놀 함량은 108~302 ㎎%, 총 플라보노이드 함량은 2.73~9.41 ㎎%였다. 이소플라본 함량은 제조사별로 큰 차이를 보이면서 genistein 63.26~217.16 ㎍/g, daidzein 58.24~166.65 ㎍/g, genistin 2.66~55.68 ㎍/g, glycitein 12.26~17.82 ㎍/g 등으로 나타났다. 관능적 품질평가(7점 채점법)에서 20~30대 패널들은 시료의 색, 냄새, 맛 등 전반적 기호도 평가에서 3.20~4.05 수준으로 비교적 낮은 평점을 나타내었다. Five different commercial chungkukjang powders (A~E) were compared based on their physicochemical, organoleptic and functional properties. The proximate composition of the five samples ranged from 6.07 to 8.54% in moisture, 15.31 to 27.07% in crude protein, 20.19 to 24.75% in crude lipid, 34.84 to 52.41% in carbohydrate, and 3.69 to 5.26% in ash. The pH of the samples ranged from 5.58 to 6.11, and Hunter's colors showed 70.01~77.22 for L value, 0.91~4.64 for a value and 23.72~31.00 for b value depending on the product. The microbial counts were 8.16~9.60 log CFU/g for aerobic bacteria, ~4.16 log CFU/g for yeasts & molds, and 1.07~3.88 log CFU/g for coliforms. The contents of reducing sugars and amino-N were 1.89~2.41% and 2.83~7.31%, respectively. Free amino acids were mainly composed of glutamic acid, valine, leucine, phenylalanine, and lysine. The amounts of total phenolics and total flavonoids were 108~302 ㎎% and 2.73~9.41, respectively, with some variations in the products. However, the isoflavone contents, which were composed of genistein (63.26~217.16 ㎍/g), daidzein (58.24~166.65 ㎍/g), genistin (2.66~55.68 ㎍/g), and glycitein (12.26~17.82 ㎍/g), were apparently different per product. The sensory scores for color, smell, taste and overall preference for the five chungkukjang products, which were evaluated by panels in their 20's and 30's using 7-point scoring test, ranged from 3.20 to 4.05.

알코올을 투여한 당뇨 흰쥐의 혈당과 혈청지질에 미치는 홍삼 청국장 추출물의 영향

이상일(Sang-Il Lee),신진기(Jin-Gi Shin),김순동(Soon-Dong Kim) 한국식품영양과학회 2005 한국식품영양과학회지 Vol.34 No.9

홍삼청국장 추출물(RC)이 알코올을 섭취중인 당뇨흰쥐 혈당과 혈청지혈에 미치는 효과를 관찰하기 위하여 SD계 흰쥐에 streptozotocin(STZ)을 투여하여 고혈당과 고지혈증을 유발시킨 다음 홍삼청국장 추출물을 함유(g/mL)하는 20%알코올을 매일 2 mL씩 2주간 경구투여하였을 때의 증체량, 식이효율, 음용수 섭취량과 배뇨량, 장기중량, 혈당 및 혈청지질 함량에 미치는 영향을 조사하였다. 그 결과 RC의 식이는 STZ 투여에 의하여 증가되었던 음용수 섭취량과 배뇨량을 개선시키지 못하였으나, 감소하였던 증체량과 식이 효율은 대조군 수준으로 회복시켰으며, 고혈당과 고지혈증도 유의하게 감소시켰다. RC는 당뇨 유발 후 에탄올 투여로 인한 사망률을 현저히 감소시켰다. 이러한 결과와 문헌상의 지견들을 종합해 볼 때, RC는 고혈당 및 고지혈증을 치료 및 예방함으로서 에탄올 섭취에 의한 사망률을 감소시킴과 동시에 당뇨에 의해 발생할 수 있는 당뇨 합병증을 예방 내지는 지연시켜주는 작용이 있을 것으로 생각된다. To evaluate red ginseng-chungkukjang extracts (RC) on levels of blood sugar and serum lipids in diabetes rats fed with ethanol, SD rats were supplemented 2 mL of 20% ethanol solution with or without RC by gastric intubation for 2 weeks after streptozotocin (STZ) injection and then body weight gains, food efficiency ratio (FER), water intake, urine volume, organ weight, levels of blood sugar and serum lipids were determined. Water intake and urine volume were not restored in STZ-treated rats by RC supplementation. On the other hand, decreased body weight gain and FER were restored in diabetes rats by RC supplementation. Furthermore, levels of blood sugar, serum triglyceride, total cholesterol and LDL-cholesterol were significantly decreased by RC supplementation. The rate of mortality in diabetes rats was significantly inhibited by RC supplementation. These results suggest that inhibited rate of mortality in diabetes rats by supplementation of ethanol with RC was considered to be due to improvement of blood sugar and serum lipids levels by components of RC.

콩 식이섬유로부터 추출한 헤미셀룰로즈의 식이가 Streptozotocin유도 당뇨 흰쥐의 혈당과 혈청 콜레스테롤 함량에 미치는 영향

이명예(Myung-Ye Lee),김미경(Mee-Kyung Kim),신진기(Jin-Gi Shin),김순동(Soon-Dong Kim) 한국식품영양과학회 2004 한국식품영양과학회지 Vol.33 No.7

콩 식이섬유로부터 제조한 헤미셀룰로즈가 streptozotocin으로 유발한 당뇨쥐의 혈당과 혈청콜레스테롤함량에 미치는 영향을 조사하였다. 실험군은 셀룰로즈군(대조군), 헤미셀룰로즈 0.5% 식이군(H-1군), 헤미셀룰로즈 1% 식이군(H-2군)으로 나누어 6주간 사육한 후 당뇨를 유발시키고 계속해서 1주간 사육하였다. 당뇨유발에 따른 식이섭취량과 체중 증가량 및 식이효율은 H-2 식이군이 대조군과 H-1군에서 보다 높은 값을 나타낸 반면 음용수 섭취량은 H-2군에서 낮았다. 간장의 무게는 H-2군이 대조군과 H-1군에 비하여 유의적으로 낮았다. 혈당은 대조군에서는 212.8 ㎎/dL, H-1군에서는 160.5 ㎎/dL, H-2군에서는 141.0 ㎎/dL으로 H-2군은 대조군의 66%수준이었으며 뇨당은 대조군 0.97 ㎎/dL, H-1군 0.53 ㎎/dL, H-2군 0.35 ㎎/dL로 H-1군은 대조군의 55%, H-2군은 대조군의 36%로 헤미셀룰로즈 식이군이 대조군에 비하여 유의적으로 낮았다. 혈청내 중성지질의 함량은 식이에 따른 유의적인 차이는 보이지 않았으나 혈청 총콜레스테롤 함량은 대조군에서 101.6 ㎎/dL, 식이군에서는 73.8~78.4 ㎎/dL로 유의적으로 낮았으며, 혈청 HDL-콜레스테롤 함량은 모든 실험군에서 39.8~44.7 ㎎/dL 범위로 비슷한 수치를 나타내었다. HTR과 동맥경화지수는 대조군에서는 각각 0.44 및 1.27로 나타났으나 헤미셀룰로즈 식이군에서는 각각 0.54 및 0.46~0.85로 대조군보다 낮았다. 이상의 결과, 콩 식이섬유로부터 추출한 헤미셀룰로즈는 항당뇨효과가 높음을 나타내었다. The effect of hemicellulose extracted from soy fiber on the level of blood glucose and serum cholesterol in the streptozotocin-induced diabetic rat were investigated. The experimental plots were divided to cellulose group (control), 0.5% hemicellulose group (H-1) and 1% hemicellulose group (H-2) group. The groups were fed for 6 weeks, then fed for 1 week more after streptozotocin injection. Food intakes, weight gain and food efficiency ratio of H-2 group were higher, while water intakes and liver weight were lower than those of control and H-1 group. The content of blood glucose and urine glucose were 212.8 ㎎/dL, 0.97 ㎎/dL in the control group, 160.5 ㎎/dL, 0.53 ㎎/dL in the H-1 group, 141.0 ㎎/dL, 0.35 ㎎/dL in the H-2 group, respectively. There was no significantly difference in the content of neutral lipid, while the content of total serum cholesterol was 101.6 ㎎/dL in the control group, 73.8~78.4 ㎎/dL in the hemicellulose groups. The content of serum HDL-cholesterol in the all experimental groups showed no significantly difference showing 39.8~44.7 ㎎/dL. HTR and atherogenic index were 0.44 and 1.27 in the control group, but 0.54 and 0.46~0.85 in the hemicellulose groups, respectively.

Scopolamine 유발 기억 손상 마우스 모델에서 국산 산사와 중국산 산사의 항건망 효과 비교

이지혜(Jihye Lee),김혜정(Hye-Jeong Kim),이찬희(Chanhee Lee),박상혁(Sang Hyeok Park),정철종(Chul Jong Jung),백경연(Gyung Yun Beik),신진기(Jin Gi Shin),정지욱(Ji Wook Jung) 한의병리학회 2018 동의생리병리학회지 Vol.32 No.6

소나무 재생버섯(Fomitopsis pinicola Jeseng) 다당류의 추출 특성

장경호,신진기,이명예,이상일,김정숙,오승희,김순동 동아시아식생활학회 2005 동아시아식생활학회지 Vol.15 No.1

This study was conducted to investigate the extraction characteristics of the polysaccharide from Jeseng mushroom (Fomitopsis pinicola Jeseng). Yields of the polysaccharide extracted from powdered mushroom by autoclaving(120, 30 min) with water at different pH and salt concentration were 8.2~9.2% in pH 5~11, 4.7~5.5% in 1.5% salt solution, respectively. The yield by the 0.05~1.0 N KOH-extraction was ranged 3.45~13.20%, while that by HAS-extraction(homogenizing after KOH swelling) using 1~2.5 N KOH 73.6~78.4%. Content of carbohydrate, protein, lipid and ash of the crude polysaccharide extracted from fruits body and its cultured mycelium by method of water extraction, KOH extraction(0.005~1N) and HAS-extraction were ranged 86.5~92.6%, 2.3~13.1%, 0.1~4.2% and 0.1~1.7%, respectively. The polysaccharide were composed of 62.0~77.8 g/g of pentose, 138.0~187.8 g/g of hexose and 21.2~117.3 ㎎/g of protein. From these results, the polysaccharide extracted was supposed to be a protein-bound polysaccharide.