PMMA를 이용한 다공질 β-TCP 골충진제 제조 및 생체적합성 평가

송호연,윤민호,김영희,민영기,양훈모,이병택,Song, Ho-Yeon,Youn, Min-Ho,Kim, Young-Hee,Min, Young-Ki,Yang, Hun-Mo,Lee, Byong-Taek 한국재료학회 2007 한국재료학회지 Vol.17 No.6

Porous ${\beta}-tricalcium$ phosphate $({\beta}-TCP)$ bioceramic was fabricated by pressureless sintering using commercial HAp and different volume percentages of PMMA powders (30-60 vol.%). The range of spherical pore size was about $200-250\;{\mu}m$ in diameter. By increasing the PMMA content, the number of pores and their morphology were dramatically changed as well as decreased the material properties. In case of using 60 vol.% PMMA content, network-type pores were found, due to the necking of the PMMA powders. The values of relative density, elastic modulus, bending strength and hardness of the 60 vol.% PMMA content sample, sintered at $1500^{\circ}C$, were about 46%, 22.2 GPa, 5MPa and 182 Hv respectively. Human osteoblast-like MG-63 cells and osteoclast-like Raw 264.7 cells were well grown and fully covered all of the porous ${\beta}-TCP$ bodies sintered at $1500^{\circ}C$.

방전플라즈마소결법에 의해 제조된 저탄성 타이타늄 다공질체의 생체적합성 평가

송호연,김영희,장세훈,오익현,Song, Ho-Yeon,Kim, Young-Hee,Chang, Se-Hun,Oh, Ik-Hyun 한국재료학회 2007 한국재료학회지 Vol.17 No.2

Porous Ti compacts were fabricated by spark plasma sintering (SPS) method and their in vitro and in vivo biocompatibilities were investigated. Alkaline phosphatase (ALP) activity representing the activity of osteoblast was increased when osteoblast-like MG-63 cells were cultured on the Ti powder surface. Some genes related to cell growth were over-expressed through microarray analysis. The porous Ti compact with 32.2% of porosity was implanted in the subcutaneous tissue of rats to confirm in vivo cytotoxicity. 12 weeks post-operation, outer surface and inside the porous body was fully filled with fibrous tissue and the formation of new blood vessels were observed. No inflammatory response was confirmed. To investigate the osteoinduction, porous Ti compact was implanted in the femur of NZW rabbits for 4 months. Active in-growth of new bone from the surrounded compact bone was observed around the porous body. From the results, The porous Ti compacts fabricated by spark plasma sintering might be available for the application of the stem part of artificial hip joint.

마이크로웨이브법에 의해 제조된 HAp 및 BCP 분말이 뼈모세포 및 파골세포의 활성에 미치는 영향

송호연,민영기,양훈모,맹주양,Song, Ho-Yeon,Min, Young-Ki,Yang, Hun-Mo,Mang, Joo-Yang 한국재료학회 2007 한국재료학회지 Vol.17 No.12

Hydroxyapatite (HAp) and biphasic calcium phosphate (BCP) nano powders were synthesized using the microwave-assisted synthesis process dependent on pH and microwave irradiation time. The average size of a powder was less than 100 nm in diameter. Through in-vitro cytotoxicity tests by an extract dilution method, the HAp and BCP nano powders have shown to be cytocompatible for L-929 fibroblast cells, osteoblastlike MG-63 cells and osteoclast-like Raw 264.7 cells. The activation of osteoblast was estimated by alkaline phosphatase (ALP) activity. When the HAp and BCP were treated to MG-63 cells, alkaline phosphatase activities increased on day 3, compared with those of the untreated cells. Also, the collagen fibers increased when the HAp and BCP powders suspension were treated to MG-63 cells, compared to those of the untreated cells. Quantitative alizarin red S mineralization assays showed a trend toward increasing mineralization in osteoblast cultured with powder suspension. In conclusion, hydroxyapatite and biphasic calcium phosphate appeared to be a bone graft substitute material with optimal biocompatibility and could be further applied to clinical use as an artificial bone graft substitute.

결핵균 특이 TB-14 재조합 단백질의 분리 및 세포성 면역반응에 미치는 영향

송호연 ( Ho Yeon Song ),김영희 ( Young Hee Kim ),김창환 ( Chang Hwan Kim ),민영기 ( Young Ki Min ),김대중 ( Dae Joong Kim ),고광균 ( Kwang Kjune Ko ) 대한결핵 및 호흡기학회 2006 Tuberculosis and Respiratory Diseases Vol.61 No.3

연구배경: Mycobacteria 배양액 여과 단백질은 결핵에 대한 세포성 면역반응 및 진단 연구에 중요한 역할을 하는 것으로 알려져 있다. 새로운 결핵균 특이 유전자를 클로닝하여 약 14-kDa의 결핵균 재조합 단백질(TB-14)을 분리 정제 한 뒤 전혈 배양(whole blood culture)을 통해 재조합 단백질 항원의 자극에 따른 IFN-γ 분비 유도를 PPD와 비교 측정하여 TB-14 단백질이 결핵균에 대한 숙주의 세포성 면역 반응 유도에 어떤 역할을 하는 지를 알아보고자 하였다. 방법 : M. avium 배양 여과액에서 항혈청과 강하게 반응하는 하나의 M. avium 특이 단백질을 확인하여 아미노산 서열을 규명한 뒤 이 단백질과 높은 상동성을 나타내는 M. tuberculosis 유전자를 클로닝하여 다량의 결핵균 재조합 단백질(TB-14)을 분리 정제하였다. 재조합 단백질 TB-14에 대한 세포성 면역 반응 유도를 알아보기 위해 결핵 환자(9명), PPD 양성 정상인(7명) 및 PPD 음성 정상인(7명)들로부터 얻은 전혈(whole blood)를 RPMI로 희석한 뒤 10㎍의 PPD와 TB-14 단백질로 48시간 동안 자극하여 상층액에 분비된 IFN-γ 농도를 ELISA로 측정하였다. 결과: 1. M avium LR114F 균주의 배양 여과액내에서 M. intracellulare 항혈청에 강하게 반응하는 새로운 M. avium 특이 단백질을 규명하여 아미노산 서열을 확인하였다. 2. M. avium 특이 단백질과 상동성을 보이는 M. tuberculosis 특이 재조합 단백질인 TB-14은 148개의 아미노산으로 구성되어 있고 30개의 아미노산으로 이루어진 signal peptides를 가지며 M. avium과 78%의 상동성을 보였다. 3. PPD 양성인의 전혈 배양에서 TB-14 단백질 항원으로 자극된 군에서 PPD로 자극된 군보다 월등히 높은 IFN-γ 분비를 나타내었다. 반면 결핵환자에서는 질환의 양상이나 치료 정도에 따라 IFN-γ의 분비 양상이 일정하지 않았다. 결론: 새로운 결핵균 특이 재조합 단백질 TB-14는 결핵에 대한 인체 내 세포성 면역반응 유도에 중요한 역할을 할 수 있는 단백질이라 생각되며 특히 PPD 양성자에서 높은 IFN-γ의 분비 양상을 보여 정상인과 결핵 감염자의 감별진단에도 활용될 수 있으리라 생각된다. 또한 TB-14 단백질에 대한 항혈청을 제작하여 환자의 가검물에 분비되는 결핵균 특이 단백질을 탐색하는 결핵의 진단 연구에도 활용될 수 있을 것으로 사료된다. Background: Culture filtrate proteins secreted by mycobacteria are thought to play an important role in inducing protective immunity and to develop new methods for diagnosing tuberculosis. Methods: A culture filtrate protein of M. avium that was strongly reactive with goat antiserum against M. intracellulare was constructed. Its homologous protein (TB-14) in M. tuberculosis was cloned, expressed and purified. The inductions of IFN-γ stimulated with 10㎍ of TB-14 recombinant protein and 10㎍ PPD were estimated by using whole bloods from seven PPD (-) subjects, seven PPD (+) healthy volunteers and nine tuberculosis patients. Results: M. avium culture filtrate protein was confirmed as a hypothetical protein that was termed contig 116. A novel 14-kDa recombinant protein (TB-14) of M. tuberculosis was composed of 148 amino acids, including 30 amino acids of the signal peptide, and it showed 78% homology with M. avium. In the PPD (+) healthy volunteers, recombinant TB-14 protein strongly induced the secretion of IFN-γ in whole blood cultures. Conclusion: These results suggest that TB-14 recombinant protein might play an important role in inducing cell-mediated immunity against tuberculosis. Furthermore, TB-14 protein antigen and its antiserum will be available for the development of new diagnostic tools for tuberculosis. (Tuberc Respir Dis 2006; 61: 239-247)

의료용 생체재료의 생물학적 안전성 평가

송호연 ( Ho Yeon Song ),김영희 ( Young Hee Kim ),이병택 ( Byong Taek Lee ) 대한금속재료학회 2009 재료마당 Vol.22 No.1

Rickettsia tsutsugamushi 감염에 의한 혈관 내피세포의 손상기전에 관한연구

송호연(Ho-Yeon Song),이강순(Kang-Soon Rhee) 大韓微生物學會 1994 大韓微生物學會誌 Vol.29 No.1

은 나노 입자의 항균기전과 항균제 응용을 위한 In vivo 실험

송호연 ( Ho Yeon Song ),김영희 ( Young Hee Kim ),고광균 ( Kwang Kjune Ko ),오익현 ( Ik Hyun Oh ),이병택 ( Byong Taek Lee ) 대한금속재료학회 ( 구 대한금속학회 ) 2006 대한금속·재료학회지 Vol.44 No.6

Silver is one of the most universal antimicrobial substances. Nano-technogy enables us to expand the surface area of silver particles markedly. Silver nano-particles were successfully produced less than 10 nm in size. Nanosilver showed excellent antibacterial activities against Salmonella typhi and Pseudomonas aeruginosa around 1 ppm and Mycobacterium tuberculosis around 10 ppm. Nanosilver induced the severe morphologic changes in cell wall and cytoplasm of bacteria. Antimicrobial mechanisms of nanosilver were different according to the species of bacteria; plasmolysis in Salmonella typhi and metabolic disturbance due to ingestion of nanosilver in M. tuberculosis. To confirm the toxicity of nanosilver, four groups of mice (60 mice in total) have been fed with nanosilver suspension (0, 10, 30, 100 ppm) for 6 months. Their behaviors, weight changes and hematologic findings of mice after feeding of nanosilver showed similar results with those of the control although the mice had been fed 100 ppm of nanosilver for 6 months. From the results, silver nanoparticles probably will be available as a good and safe antibiotic alternative.

자연어처리를 기반으로 한 코로나 정보 제공 챗봇 시스템

송호연(Ho-yeon Song),이윤수(Yun-soo Lee) 한국정보통신학회 2020 한국정보통신학회 여성 ICT 학술대회 논문집 Vol.2020 No.8

생체세라믹 알루미나 분말이 뼈모세포 및 파골세포의 증식에 미치는 영향

송호연 ( Ho Yeon Song ),김영희 ( Young Hee Kim ),고광균 ( Kwang Kjune Ko ),김양희 ( Yang Hee Kim ),오익현 ( Ik Hyun Oh ),이병택 ( Byong Taek Lee ) 대한금속재료학회 ( 구 대한금속학회 ) 2006 대한금속·재료학회지 Vol.44 No.6

The main cause of re-operation in patients who received total hip arthroplasty is wear debris-mediated osteolysis in bone-implant interface. Recently, alumina or zirconia ceramic hip joint have also become available. Therefore, Al2O3 particles induced cytotoxicity on osteoblast-like MG-63 cells and osteoclastlike Raw 264.7 cells was observed through in vitro and in vivo study. Low concentration of Al2O3 powders did not induce cytotoxicity, however the proliferation of osteoblast cultured with high concentration of Al2O3 powders were significantly inhibited. Al2O3 particles were also observed on the surface and inside the MG-63 and Raw 264.7 cells accompanied with the morphological changes of cytoplasmic organelles. Al2O3 particles injected in subcutaneous tissue of rats did not induce inflammatory response.

자연어처리를 기반으로 한 코로나 정보 제공 챗봇 시스템

송호연 ( Ho-yeon Song ),곽찬우 ( Chan-woo Gwak ),이동원 ( Dong-won Lee ),이윤수 ( Yun-soo Lee ) 한국정보처리학회 2020 한국정보처리학회 학술대회논문집 Vol.27 No.2

본 논문에서는 코로나 바이러스로 인한 불편함을 겪는 사람들에게 코로나 관련 정보를 편리하게 제공하기 위해 인공지능 기반의 챗봇을 개발하였다. 인공지능 기술이 도입됨에 따라 챗봇이 여러 기관에서 고객 응대를 포함한 다양한 업무를 수행하고 있다는 점에 착안하여 IBM Watson Assistant 를 활용한다. Watson Assistant는 사용자가 입력한 말을 자연어 처리를 통해 분석하여 문장을 생성한 후 사용자에게 전달한다. Intent와 Entitiy를 통해 질의의 행위와 주체를 입력한 후 높은 빈도가 예상되는 질문을 작성하고, Dialog를 통해 대화 흐름을 파악한다. 사용자는 Axure로 설계된 사용자 인터페이스를 통해 대화 전송, 정보 수신 등 동작을 취할 수 있으며, 각 언어에 맞는 SDK 라이브러리를 제공한다는 이점을 활용하여 Node.js로 화면에서 발생하는 액션과 데이터 전달을 처리한다.