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흰쥐 후지근 피판에서 허혈-재순환 손상시 pERK1/2 발현에 대한 ${\alpha}-lipoic$ Acid의 효과
송정훈,김민선,박병림,박한수,채정룡,이혜미,나영천,Song, Jeong-Hoon,Kim, Min-Sun,Park, Byung-Rim,Park, Han-Su,Chae, Jeong-Ryong,Lee, Hye-Me,Na, Young-Cheon 대한미세수술학회 2005 Archives of reconstructive microsurgery Vol.14 No.2
Purpose: This study was to evaluate the effect of ${\alpha}-lipoic$ acid, a potent free radical scavenger, on the expression of active form of extracellular signal-regulated kinase (pERK1/2) proteins from hindlimb muscles of rats following ischemia-reperfusion injury. Material and methods: 64 health, $280{\sim}350\;g$ weighted Sprague-Dawley male rats were used. In order to make a muscle flap, the gastrocnemius (GC) and soleus (SOL) muscles were dissected and elevated. The popliteal artery was occluded for 4hours and reperfused for 10 minutes, 30 minutes, 1 hour, 2 hours and 4 hours, respectively. Results: The ischemia by occlusion of the popliteal artery itself caused a minimal change in expression of phosphorylated form of proteins observed in hindlimb muscle. In contrast, after 4 hours of ischemia, immunoreactivity for pERK1/2 in the GC muscle showed dual peaks at 10 minutes and 4 hours after reperfusion. In ${\alpha}-lipoic$ acid treated group, the expression of pERK1/2 was increased significantly compared to I/R-only group. Conclusion: These results suggest that ${\alpha}-lipoic$ acid may protect I/R injury of the skeletal muscle through free radical scavening and activation of intracellular pERK1/2 expression.