저산소/재관류로부터 청폐사간탕의 PC12 세포 보호 효과

소윤조 한국생약학회 2005 생약학회지 Vol.36 No.2

This research was performed to investigate the protective effect of Chungpesagan-tang (CST) from hypoxia /reperfusion induced-PC12 cell damage. To elucidate the mechanism of the protective effect of CST, cell viability, changes in activities of superoxide dismutase, glutathione peroxidase, catalase, caspase 3 and the production of malondialdehyde were observed after treating PC12 cells with CST which was metabolized by rat liver homogenate. Pretreatment of CST with liver homogenate appeared to increase its protective effect against hypoxia/reperfusion insult. The result showed that CST exhibited the highest protective effect against hypoxia/reperfusion at the dose of 1 µg/ml in PC12 cells, probably by recovering the redox enzyme activities and MDA to control level.

간효소에 의해 대사된 양격산화탕의 저산소/재관류로부터 PCl2 세포 보호효과

소윤조(Yunjo Soh) 大韓藥學會 2005 약학회지 Vol.49 No.1

The protective effect of Yangguksanwha-tang (YST) against hypoxia-reperfusion insult was investigated in PC12 cells. To elucidate the mechanism of the protective effect of YST, cell viability the changes in activities of superoxide dismutase, glutathione peroxidase, catalase, caspase 3 and the production of malondialdehyde were observed after treating PC12 cells with YST which was metabolized by rat liver homogenate. Pretreatment of YST with liver homogenate appeared to increase its protective effect against hypoxia-reperfusion insult. The result showed that YST had the highest protective effect against hypoxia/reperfusion at the dose of 2㎍/ml in PC12 cells, probably by recovering the redox enzyme activities and MDA to control level.

갯지렁이와 지렁이 추출물의 항염증 및 항산화 효과 비교

김세웅,소윤조,삽코타마헤쉬,이양,양명,박찬일 한국생약학회 2016 생약학회지 Vol.47 No.2

Earth worm (Eisenia andrei) and clam worm (Perinereis linea) have been used as anti-bacterial and anti-inflammatory agent. However, it is unclear how they exerted their physiological effects in macrophages. In this experiment, the anti-inflammatory and antioxidant effects of clam worm extract (CWE) and earth worm extract (EWE) in RAW264.7 cells were examined by measuring MDA, catalase, SOD, GSH-Px and inflammatory cytokines (nitric oxide, iNOS, interleukin-1b and tumor necrosis factor-a). Treatment with CWE significantly increased the activities of catalase, SOD and GSH-Px in RAW264.7 cells and decreased the level of MDA. Interestingly, treatment with CWE induced more activities of SOD than EWE. In addition, CWE decreased NO production, iNOS, COX-2, TNF-a and IL-1b in RAW264.7. The EWE also decreased NO production and iNOS, but increased COX-2 and IL-1b suggesting that CWE could be better resources for anti-inflammatory and antioxidant agent than EWE. Taken together, these results indicate that CWE has the potential as a natural antioxidant and a therapeutic for inflammation-related diseases.

Antioxidant and Anti-Inflammatory Effects of NCW Peptide from Clam Worm (Marphysa sanguinea)

박영란,박찬일,소윤조 한국미생물·생명공학회 2020 Journal of microbiology and biotechnology Vol.30 No.9

Clam worms (Marphysa sanguinea) are a rich source of bioactive components such as the antibacterial peptide, perinerin. In the present study, we explored the physiological activities of a novel NCWPFQGVPLGFQAPP peptide (NCW peptide), which was purified from clam worm extract through high-performance liquid chromatography. Tandem mass spectrometry (MS/MS) revealed that NCW was a new peptide with a molecular weight of 1757.86 kDa. Moreover, NCW peptide exhibited significant antioxidant effects, causing a 50% inhibition of DPPH radical at a concentration of 20 μM without showing any cytotoxicity. These were associated with a reduction in the activity of catalase (CAT), superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), and malondialdehyde (MDA) in LPS-stimulated RAW264. 7 cells. Furthermore, NCW peptide exhibited anti-inflammatory effects in LPS-stimulated RAW264.7 macrophages via inhibition of the abnormal production of pro-inflammatory cytokines including nitric oxide (NO), inducible nitric oxide synthase (iNOS), and cyclooxygenase-2 (COX-2). These anti-inflammatory effects of NCW peptide were associated with the inhibition of interleukin-1β (IL-1β) and tumor necrosis factor-α (TNF-α). Our results therefore suggest that this novel NCW peptide with antioxidant and anti-inflammatory effects could be a good therapeutic agent against inflammation-related diseases.

양극산화와 전석회화 처리한 Ti-6Al-4V 합금의 생체활성도

백은미,최운재,소윤조,박병건,이민호,배태성,김병일 大韓齒科器材學會 2009 대한치과재료학회지 Vol.36 No.4

The purpose of this study was to investigate the Osseoconduction of Ti-6Al-4V alloy modified by anodic oxidation and precalcification treatments. Specimens of 20 × 10 × 2 mm in dimensions were polished sequentially from #300 to #1000 SiC paper, ultrasonically washed with acetone, then rinsed with deionized water, and dried in an oven at 50℃ for 24 hours. The electrolyte solution for anodization process consisting of 0.5 M Na2SO4 and 0.4 wt% NaF. Anodization was carried out by pulse signals at a potential of 20 V and current density of 20 mA/cm2 for 3 hours. Pulse signals were adjusted on/off time with 10/90 ms and 90/10 ms during 100 ms. TiO2 nanotubes were heat-treated at 500℃ for 2 hours and precalcified by soaking in Na2HPO4 solution for 24 hours and then in saturated Ca(OH)2 solution for 5 hours. To evaluate the bioactivity of the precalcified TiO2 nanotube layer, hydroxyapatite formation was investigated in a simulated body fluid with pH 7.4 at 36.5℃ for 10 days. The results obtained were summarized as follows; 1. The structures of TiO2 nanotubes treated in pulse on/off ime 90/10 ms were more dense structure than those treated in 10/90 ms. 2. Ti, Al and Na were detected but V was not examined in iO2 nanotube layer. 3. The Osseoconduction of TiO2 nanotubes were more nhanced by precalcification treatment in the solution containing Ca2+ and PO4 3- ions.

펩티도글리칸 처리된 갯지렁이 추출물의 항염증 및 항산화 효과

김세웅,삽코타마헤쉬,양명,이양,소윤조 한국생약학회 2017 생약학회지 Vol.48 No.3

Peptidoglycan in inserts and mammals is well known to improve biological functions in the host’s immune system. However, it is unclear how Peptidoglycan exerted its anti-inflammatory capacity especially in clam worm (Marphysa sanguinea). In this experiment, the anti-inflammatory and antioxidant effects of clam worm extract treated with (PCWE) peptidoglycan (Micrococcus luteus) in RAW264.7 cells were examined by measuring MDA, catalase, SOD, GSH-Px and inflammatory cytokines (nitric oxide, iNOS, interleukin-1b and tumor necrosis factor-a). PCWE significantly increased the activities of catalase, SOD and GSH-Px and decreased the level of MDA. Interestingly, PCWE induced activities of SOD and GSH-Px more than clam worm extract without peptidoglycan (CWE). In addition, PCWE decreased NO production, iNOS, COX-2, TNF-a and IL-1b better than CWE. Taken together, these results indicate that PCWE has the potential as a natural antioxidant and a therapeutic for inflammation-related diseases.

Effects of aloe-emodin on alveolar bone in Porphyromonas gingivalis-induced periodontitis rat model: a pilot study

Ming Yang,Saroj K Shrestha,소윤조,허석모 대한치주과학회 2022 Journal of Periodontal & Implant Science Vol.52 No.5

Purpose: Aloe-emodin (AE), a natural anthraquinone abundant in aloe plants and rhubarb (Rheum rhabarbarum), has long been used to treat chronic inflammatory diseases. However, AE’s underlying mechanisms in periodontal inflammation have not been fully elucidated. Acidic mammalian chitinase (AMCase) is a potential biomarker involved in bone remodeling. This study aimed to evaluate AE’s effect on periodontitis in rats and investigate AMCase expression. Methods: Eighteen Sprague-Dawley rats were separated into the following groups: healthy (group 1), disease (group 2), vehicle (group 3), AE high-dose (group 4), and AE low-dose (group 5). Porphyromonas gingivalis ligatures were placed in rats (groups 2–5) for 7 days. Groups 4 and 5 were then treated with AE for an additional 14 days. Saliva was collected from all groups, and probing pocket depth was measured in succession. Periodontal pocket tissues were subjected to histomorphometric analysis after the rats were sacrificed. Bone marrowderived macrophages and murine macrophages were stimulated with receptor activator of nuclear factor-κB ligand (RANKL) and treated with different concentrations of AE. AMCase expression was detected from the analysis of saliva, periodontal pocket tissues, and differentiated osteoclasts. Results: Among rats with P. gingivalis-induced periodontitis, the alveolar bone resorption levels and periodontal pocket depth were significantly reduced after treatment with AE. AMCase protein expression was significantly higher in the disease group than in the healthy control (P<0.05). However, AE inhibited periodontal inflammation by downregulating AMCase expression in saliva and periodontal pocket tissue. AE significantly reduced RANKLstimulated osteoclastogenesis by modulating AMCase (P<0.05). Conclusions: AE decreases alveolar bone loss and periodontal inflammation, suggesting that this natural anthraquinone has potential value as a novel therapeutic agent against periodontal disease.

Hexane-Soluble Fraction of the Common Fig, Ficus carica, Inhibits Osteoclast Differentiation in Murine Bone Marrow-Derived Macrophages and RAW 264.7 Cells

박영란,은재순,최화정,네팔마노즈,김대근,서승용,Rihua Li,문우성,조남표,조성대,배태성,김병일,소윤조 대한약리학회 2009 The Korean Journal of Physiology & Pharmacology Vol.13 No.6

Osteoclasts, derived from multipotent myeloid progenitor cells, play homeostatic roles in skeletal modeling and remodeling, but may also destroy bone in pathological conditions such as osteoporosis and rheumatoid arthritis. Osteoclast development depends critically on a differentiation factor, the receptor activator of NF-κB ligand (RANKL). In this study, we found that the hexane soluble fraction of the common fig Ficus carica (HF6-FC) is a potent inhibitor of osteoclastogenesis in RANKL-stimulated RAW264.7 cells and in bone marrow-derived macrophages (BMMs). HF6-FC exerts its inhibitory effects by suppression of p38 and NF-κB but activation of ERK. In addition, HF6-FC significantly decreased the expression of NFATc1 and c-Fos, the master regulator of osteoclast differentiation. The data indicate that components of HF6-FC may have therapeutic effects on bone-destructive processes such as osteoporosis, rheumatoid arthritis, and periodontal bone resorption.

오가피(Eleutherococcus sessiliflorus )의 전연골성 ATDC5 세포의 분화 유도

스레스타사로즈쿠마 ( Saroj Kumar Shrestha ),송정빈 ( Jungbin Song ),이성현 ( Sung Hyun Lee ),이동헌 ( Donghun Lee ),김호철 ( Hocheol Kim ),소윤조 ( Yunjo Soh ) 대한본초학회 2022 大韓本草學會誌 Vol.37 No.1

Objectives : The process through which mesenchymal cells condense and differentiate into chondrocytes to form new bone is known as endochondral bone formation. Chondrogenic differentiation and hypertrophy are essential steps in bone formation and are influenced by various factors. The stem bark and root bark of Eleutherococcus sessiliflorus (ES) have been widely used to treat growth retardation and arthritis in traditional Korean Medicine. In this study, we aimed to investigate the possible role of the stem bark of ES in the stimulation of chondrogenic differentiation in clonal murine chondrogenic ATDC5 cells. Methods : In ATDC5 cells treated with ES extract, cell viability and extracellular matrix production were determined using CCK-8 assay and Alcian blue staining, respectively, and alkaline phosphatase activity was measured. We also examined mRNA and protein expression levels of genes related to chondrogenic expression in ATDC5 cells using reverse transcription-polymerase chain reaction and western blot analyses. Results : ES extract increased the accumulation of Alcian blue-stained cartilage nodules and alkaline phosphatase activity in ATDC5 cells. It increased the mRNA expressions of chondrogenic markers including bone sialoprotein (BSP), cartilage collagens, Runt-related transcription factor-2 (RUNX-2), osteocalcin (OCN), β-catenin, and bone morphogenetic protein-2 (BMP-2), as well as the protein expressions of β-catenin, RUNX-2, BMP-2, and alkaline phosphatase (ALP). Conclusion : Taken together, these results suggest that ES extract exhibits a chondromodulating activity and therefore may be a possible agent for the treatment of bone growth disorders.

석회화 전처리가 양극산화 처리한 타이타늄 임플란트의 생체활성도에 미치는 영향

박소영(So-Young Park),박일송(Il-Song Park),오상훈(Sang-Hoon Oh),정회웅(Hoi-Wung Chung),소윤조(Yun-Jo Soh),김형섭(Hyung-Seop Kim) 대한치과재료학회 2011 대한치과재료학회지 Vol.38 No.4