HT-29 대장암세포에서 Akt 활성 저해에 따른 셀레늄의 세포 증식억제 효과

Song Yi Park(박송이),In-Seop Kim(김인섭),Se Hee Lee(이세희),Sol Hwa Lee(이솔화),Da Woon Jung(정다운),Ock Jin Park(박옥진),Young Min Kim(김영민) 한국생명과학회 2012 생명과학회지 Vol.22 No.1

Akt는 세포의 증식과 분화에 관여하며 많은 암종에서 과발현되어 있다는 것이 보고되었다. 본 연구에서는 Akt의 조절을 통한 셀레늄의 HT-29 세포의 세포증식억제 시너지효과를 확인하였다. 셀레늄을 농도별과 시간별로 처리하였을 때 HT-29 세포의 증식이 억제되었고, apoptosis가 일어남을 확인하였다. 셀레늄을 농도별로 처리하여 Western blotting 및 immunofluorescence를 실시한 결과 Akt의 인산화가 저해되었고 COX-2의 발현도 저해되었다. 또한 Akt 저해제인 LY294002를 처리한 결과, HT-29 대장암세포의 증식이 억제되었으며, LY294002를 셀레늄과 병행처리하였을 때 셀레늄에 의한 세포증식억제 효과가 더 강하게 나타나는 것을 확인하였다. Akt siRNA에 의한 Akt의 불활성화는 non-transfected 세포에 비하여 HT-29 세포의 성장을 더 강하게 억제하였으며, Akt가 불활성화 되었을 때 COX-2의 발현 역시 non-transfected 세포에 비하여 감소된 것을 확인하였다. 따라서 HT-29 세포에서 셀레늄의 세포증식억제 효과는 Akt와 COX-2 신호분자의 조절을 통해 일어나며, Akt 의 저해는 셀레늄의 대장암세포증식 억제에 시너지 효과를 나타냄을 확인하였다. Akt is known to play an important role in cell proliferation and differentiation, and is also over-expressed in several types of cancer cells. In this study, we explored the anti-proliferative effects of selenium in HT-29 colon cancer cells, mediated through effects on Akt and COX-2. Selenium treatments at different concentrations and for different durations inhibited proliferation of HT-29 colon cancer cells and increased apoptotic cell death. Selenium treatment decreased Akt phosphorylation and COX-2 expression. Treatment with LY294002 (an Akt inhibitor) decreased proliferation of HT-29 cells, while a combined treatment with LY294002 and selenium resulted in even further decreases in cell proliferation. Inactivation of Akt by Akt siRNA treatment abolished these inhibitory effects on cell growth. COX-2 expression decreased in Akt transfected cells compared to non-transfected cells. These results suggest that selenium induced both anti-proliferative and apoptotic effects by inhibiting Akt phosphorylation and COX-2 expression. Selenium treatment also appeared to induce synergistic anti-proliferative effects by inhibition of Akt in HT-29 colon cancer cells.

전라남도 지역의 하천수에 존재하는 내분비 장애물질(Bisphenol A와 Styrene oligomer)의 실태조사

박송인 ( Song In Park ),정선용 ( Seon Yong Chung ),( Saido Kstsuhiko ),( Sato Hideto ),나숙현 ( Suk Hyun Na ) 한국물환경학회 2012 한국물환경학회지 Vol.28 No.5

Recently, there have been active researches regarding endocrine-disrupting chemicals (EDCs). In this study, fifteen small freshwater streams in Cholla-namdo province, South Korea were investigated with respect to the concentration of the endocrine disruptors - Bisphenol A (BPA), styrene monomer (SM), styrene dimer (SD), and styrene trimer (ST) by gas chromatography-mass spectrometry (GC-MS). Measured concentration of the target compounds in the sampled water ranged from <ND to 5.03 ng/mL, <ND to 0.1 ng/mL, <ND to 0.04 ng/mL, and ND to 0.45 ng/mL for BPA, SM, SD, and ST, respectively. The endocrine disruptors analysis of the water samples showed that the concentration of BPA and ST were much higher than the other two compounds. These results imply that the streams of Cholla-namdo province were contaminated by materials generated from decomposition of polystyrene (PS) chemically and physically.

응급의학과 전문의 총조사 자료를 이용한 응급의학과 의사의 남녀 차이

배경수 ( Kyung Su Bae ),박경혜 ( Kyung Hye Park ),강지훈 ( Ji Hun Kang ),기동훈 ( Donghune Key ),김인병 ( In Byung Kim ),김홍재 ( Hong-jae Kim ),박송이 ( Song Yi Park ),서범석 ( Beom Sok Seo ),여인환 ( In Hwan Yeo ),윤유상 ( Yoo S 대한응급의학회 2022 대한응급의학회지 Vol.33 No.4

Objective: In this study, we compared differences based on the gender of Korean emergency physicians to provide basic data for future emergency medicine development plans. Methods: We used data from the emergency physician survey (2020) conducted by the Korean Society of Emergency Medicine. A total of 1,303 emergency physicians responded to the survey. We adjusted demographic data relating to age, position, and work duration as an emergency physician, which could affect the differences based on gender. After propensity score matching, we compared working hours, work burden, income, motives for applying for emergency medicine, and future plans of the emergency physicians by sex. Results: The total working hours per month and night shifts per month were higher in men compared to the women. The women felt more pressure in the following areas: number of emergency patients, relationships with administrative agencies, insufficient emergency medical technician manpower, patients’ compliance, and relationships with other emergency physicians. Monthly income was higher in men. Women placed a higher value on ‘Acquiring a range of knowledge’ and ‘Degree of interest in working in the emergency room’ than men as motivation for applying for positions in emergency medicine. Regarding future plans, women placed less value on income and more on having enough free time and avoiding burnout. The retirement age was about 3 years lower for women than for men. Conclusion: This study revealed gender differences in working hours, income, motivation for applying for emergency medicine, work burden, and future plans. It is expected that these differences are caused by physical limitations and socio-cultural backgrounds. It is recommended that more detailed investigations be carried out through qualitative research. These results could help in gender considerations while hiring emergency physicians.

숲속 공기와 주변 우점수목 정유의 모노테르펜류 분포 비교

오길영 ( Gil Young Oh ),서윤규 ( Yun Gyu Seo ),박귀환 ( Gui Hwan Park ),김익산 ( Ik San Kim ),배주순 ( Ju Soon Bae ),박송인 ( Song In Park ),하훈 ( Hun Ha ),양수인 ( Soo In Yang ),이지헌 ( Ji Hun Lee ),이완진 ( Wan Jin Lee ) 한국산림과학회 2013 한국산림과학회지 Vol.102 No.3

The concentration of terpene (18 species) was measured from spring to fall in ambient air and essential oil at three different forests located in Jeollanamdo province. Sampling was conducted at 4-hour-interval a day of each season. In the ambient air, α-pinene, β-pinene and 2-carene were the most abundant compounds throughout the sampling periods and areas. The highest three-season average concentration of total terpene was 2,597 pptv at Jeamsan(Mt.) in Boseong which was predominantly populated by Pinaceae. The seasonal averages were observed to peak during spring with their max at Palyeongsan(Mt.) in Goheung and Jeamsan(Mt.) in Boseong, and during fall at Woodland in Jangheung. Most of terpenes had diurnal variations with higher concentrations during the daytime, and lower during the nighttime. However in essential oil, the highest annual average concentration of total terpene was 798 μg/dry-g at Goheung which was predominantly populated by Chamaecyparis obtusa. Also, the component proportion ratio showed different distribution between ambient air and essential oil. From the results of this study, we suggest that Chamaecyparis obtusa species is more useful, and younger tree is more economical than older one, and spring is the best season for lumbering in order to use essential oil.

도내 산단 주변 하천의 수질 특성 및 생태독성 평가 연구

신혜정 ( Hye-jeong Shin ),박송인 ( Song-in Park ),이재령 ( Jae-ryung Lee ),허동화 ( Dong-hwa Heo ),지창학 ( Chang-hak Ji ),김양기 ( Yang-gi Kim ) 한국환경기술학회 2023 한국환경기술학회지 Vol.24 No.2

The purpose of this study is to evaluate quality of water on rivers in the Yeosu National Industrial Complex and to analyze the water quality factors that cause ecotoxicity. The subjects of the research were Namsucheon 4 stations, Jungheungcheon 2 stations and Sangamcheon 2 stations of Yeosu National Industrial Complex in Jeollanam-do. In addition, as a control group, water environment monitoring 8 stations in Jeollanam-do were selected for analysis. Physicochemical items and ecotoxicity were analyzed according to the Water Pollution Process Test Standards. Analysis results of water pollution items, BOD was 0.1 mg/L ~ 3.0 mg/L, TOC was 0.2 mg/L ~ 12.4 mg/L, SS was 0.4 mg/L ~ 72.0 mg/L, and T-N was 0.28 mg/L ~ 3.70 mg/L, T-P was 0.005 mg/L ~ 1.526 mg/L, total coliform was 0 ~ 1,200,000 total coliforms/100 mL, ABS was not detected ~ 0.15 mg/L, cyan was not detected ~ 0.07 mg/L, and organic phosphorus and phenols were not detected at all points. In terms of heavy metal analysis results, Mn was not detected ~ 0.505 mg/L, Fe was not detected ~ 1.633 mg/L, Zn was not detected ~ 0.733 mg/L, Cu was not detected ~ 0.014 mg/L, Ba was not detected ~ 0.052 mg/L, and seven items including Hg were not detected. Cyan was detected seven consecutive times in range of 0.01 mg/L ~ 0.07 mg/L at the H point. Compared with the 8 points of the water environment monitoring stations selected as the control group, the 8 points of the rivers around the Yeosu National Industrial Complex stations were managed relatively well, as there are no peculiarities. We calculated the toxicity value and evaluated the toxicity level of the river around the Yeosu National Industrial Complex using Daphnia magna. We expect that the result of this study will be used as basic data when government establish the policies of comprehensive management for rivers in the future.

계곡지역의 산소음이온 분포

김익산(Ik San Kim),이덕안(Duk An Lee),박송인(Song In Park),하훈(Hoon Ha),양수인(Su In Yang) 大韓環境工學會 2014 대한환경공학회지 Vol.36 No.4

계곡지역에서 산소음이온 분포 특성을 고찰하기 위해 전남소재의 유명계곡 4곳을 선정하였다. 각 지역별 산소음이온의 최대값은 11,190~178,100 ea/mL이고, 평균값은 7,020~14,060 ea/mL으로 나타났다. 하루 중 오전 5시~7시경에 가장 높았고, 오후 12시~14시경에 가장 낮은 값을 보였다. 발생원인 폭포와의 이격거리에 따라 남창과 대흥사지점은 7 m까지, 어치와 수락지점은 하방 50 m까지 그 영향이 미치는 걸 알 수 있었다. 기상조건과 음이온 발생과의 상관계수 산정결과 풍속과 -0.54, 습도와는 0.34를 보였다. 풍속은 장수마을(2011년)과 섬지역(2009년)에서는 음이온 발생과 상관이 없었고, 습도는 섬지역과 유사한 상관성을 나타냈다. 따라서 바람이 없는 이른 아침에 계곡주변을 산책하는 것만으로도 요즘 유행하는 웰빙생활이라 할수 있겠다. This study was carried out to investigate the anion-distribution of the famous valleys in Jeollanam-do. Sampling sites were the valley region of Eochi, Surak, Daeheungsa and Namchang. The maximum values of the anion level were 11,190~178,100 ea/mL at each site and the highest value was measured at the Surak. The mean values showed 14,060 ea/mL in Surak, 8,590 in Eochi, 8,420 in Daeheungsa and 7,020 in Namchang. For a day, the highest Concentration showed in the 5:00 a.m to 7:00 a.m and the lowest values showed in the 12:00 p.m to 14:00 p.m. According to the distance from the source, the anion tended to disappear within 7 m in Namchang and Daeheungsa. But, it influenced by 50 m in Eochi and Surak. Correlation coefficient was 0.54 with wind speed, 0.34 with humidity about the formation of anion. There was no significant correlation with wind speed in the island and longevity village where we had studied in 2009 and 2011. But there was similar correlation with humidity in the island region. Therefore, we can say that wellbeing life is walking around the valley early in the morning with no wind.

산업폐수의 총유기탄소(TOC) 특성 - 전남 동부권 사업장을 중심으로 -

박보영 ( Bo-young Park ),문희 ( Hee Moon ),허동화 ( Dong-hwa Heo ),전수인 ( Su-in Jeon ),신혜정 ( Hea-jung Sin ),박송인 ( Song-in Park ),김양기 ( Yang-gi Kim ) 한국환경기술학회 2022 한국환경기술학회지 Vol.23 No.6

TOC was applied to the effluent new standard as a substitute of COD_Mn. But the setting of the TOC did not reflect the characteristics of various wastewater, such as the treatment facility size and the discharge type. This study assessed each industrial facility in terms of water quality characteristics by replacing COD_Mn with TOC. In the study, 96 data groups across ten industrial categories based on the Korean Standard Industrial Classification were measured COD_Mn and TOC to obtain data about waste water discharge facilities in the eastern parts of Jeollanam-do province. The measurements show that the mean COD_Mn and TOC concentration were in the ranges of 4.2 mg/L ∼ 82.0 mg/L and 3.7 mg/L ∼ 195.4 mg/L, respectively. The relative ratio(COD_Mn/TOC) was 0.42 ∼ 1.50 and the oxidation rate of COD_Mn was 12.69 % ∼ 45.45 %. The TOC emission rate against the acceptable emission criteria was in the range of 7.73 % ∼ 100.72 %. The result of TOC and COD_Mn was 82.0 mg/L and 195.4 mg/L in other food manufacturing facilities, 8.5 mg/L and 14.2 mg/L in basic petrochemical compound manufacturing facilities, 24.8 mg/L and 25.7 mg/L in transportation equipment repair and car washing or cleaning facilities. Correlations among different organic matters were analyzed according to business categories, and the results show a range of 0.9813 ∼ 0.3742, which points to huge differences among business categories. In addition, the study examined 10 items in 4 Establishments, whose wastewater discharge was less than 200 m3. The result showed that their organic matter discharges had been underestimated as they were in the food industry. These results raise a concern with the possibilities of their exceeding the criteria in case of TOC application and a need for them to improve their processes and consequently change their management methods.

레스베라트롤의 HT-29 대장암 세포증식 및 이동성 억제효과

이솔화(Sol Hwa Lee),박송이(Song Yi Park),김인섭(In-Seop Kim),박옥진(Ock Jin Park),김영민(Young Min Kim) 한국생물공학회 2012 KSBB Journal Vol.27 No.5

Resveratrol, natural polyphenol in grapes and red wine, is known to have the anti-proliferatory and anti-angiogenic effects in various cancer cells. In this study, we have investigated the effects of resveratrol in HT-29 colon cancer cells. Treatment of resveratrol in different concentrations and time inhibited proliferation of HT-29 colon cancer cells. We explored the effects of resveratrol on HT-29 colon cancer cell motility using a wound healing assay. In the absence of the resveratrol, the HT-29 cells are migrated along the edges of the wound and showed a large-scale migration, whereas dose- and time-dependent inhibition of cell flattening and spreading was observed in the presence of resveratrol. Resveratrol inhibited MMP-9 in a dose- and time-dependent on HT-29 colon cancer cells by Western blotting. In addition, resveratrol increased AMPK activity and decreased COX-2, VASP and VEGF expression. Treatment of compound C inhibited AMPK activity, however, the expression of VASP and COX-2 increased thus, COX-2 and VASP are modulated by AMPK. However treatment of celecoxib could not control AMPK activity but decreased VEGF expression. We suggest that resveratrol inhibits cell proliferation and migration through activation of AMPK and decreased COX-2, VASP and VEGF expression in HT-29 colon cancer cells.

「가고 싶은 섬」의 갯벌과 토양환경 특성(Ⅵ) - 금당도, 금호도 중심으로 -

유휘선 ( Yoo Hwi-sun ),양정고 ( Yang Jung-go ),박송인 ( Park Song-in ),박혜진 ( Park Hea-jin ),김수진 ( Kim Su-jin ),김경수 ( Kim Kyung-su ) 한국도서(섬)학회 2022 韓國島嶼硏究 Vol.34 No.1

섬은 해양자원과 역사·문화적 가치 및 영토적 중요한 자산으로 활용가능성이 높은 미래 성장의 원천이라 할 수 있다. 이에 전라남도는 섬들의 비교우위 자원 개발 및 섬만의 콘텐츠 발굴을 위해 「가고 싶은 섬」가꾸기 10개년 계획(2015~2024)을 수립하여 추진하고 있다. 특히 2021년은 섬에 대한 체계적인 조사 · 연구, 정책수립 진흥 업무를 수행하기 위한 「한국섬진흥원」이 목포에 개원하였다. 2021년 「가고 싶은 섬」 가꾸기 사업의 일환으로 완도 금당도와 진도 금호도가 선정되어 섬의 비교우위 자원을 찾기 위한 연구를 추진하였으며 2015년부터 추진해온 결과를 검토해 본 결과, 섬 지역은 육지에 비해 인체에 유용한 게르마늄과 셀레늄 성분이 풍부한 것으로 조사되었다. 이에 본 연구에서는 완도 금당도와 진도 금호도를 중심으로 토양 및 농·수산물의 기능성물질인 게르마늄과 셀레늄의 함량을 분석하여 전남 섬의 우수 농수산물을 발굴하고 토양오염물질 분석을 통해 청정한 전남의 섬 이미지를 확인하여 부가가치 창출에 기여하고자 수행하였다. 게르마늄과 셀레늄의 분석방법은 토양오염공정시험기준과 식품공전 및 과거논문자료를 바탕으로 ICP-MS와 ICP를 활용하여 분석하였고 그 결과 토양과 갯벌의 게르마늄 함량은 완도 금당도 1,795.6 μg/kg ∼ 3,103.3 μg/kg, 진도 금호도 1,797.3 μg/kg ∼ 2,914.4 μg/kg으로 나타났으며 금당도 평균값은 토양 2,470.9 μg/kg, 갯벌 2,124.6 μg/kg이며 금호도 평균값도 토양 2,521.7 μg/kg, 갯벌 1,797.3 μg/kg으로 두 섬 모두 토양과 갯벌에서의 함량이 유사한 것으로 조사되었다. 토양과 갯벌의 셀레늄 함량은 완도 금당도 79.1 μg/kg ∼ 517.6 μg/kg, 진도 금호도 214.4 μg/kg ∼ 978.2 μg/kg으로 나타났으며 평균 함량은 금당도 토양 359.5 μg/kg, 갯벌 146.9 μg/kg, 금호도 토양 609.6 μg/kg, 갯벌 214.4 μg/kg으로 갯벌보다 토양의 함량이 더 높게 조사되었다. 농·수산물 중의 게르마늄 성분은 금호도 도라지(388.2 μg/kg)와 더덕(218.0 μg/kg)에서 함량이 높은 것으로 분석되었으며 그 다음으로 금당도 고동(181.8 μg/kg)이 높게 분석되었다. 농·수산물 중의 셀레늄 함량은 금당도 가리비(2,735.7μg/kg)가 가장 높게 분석되었고, 그 다음으로 금당도 굴(1,617.8 μg/kg), 금호도 꽃게(969.3 μg/kg) 순으로 분석되었다. 금당도와 금호도의 토양오염도 조사결과 토양오염우려기준(1지역) 이내로 청정한 환경을 유지하고 있으며 이를 활용한 부가가치창출이 가능할 것으로 판단된다. 금호도의 더덕과 도라지는 다른 작물에 비해 게르마늄 함량이 높고 금당도의 가리비의 경우 셀레늄 함량이 높으며 또한 게르마늄 또한 함유하고 있으므로 이를 활용한 우수 농수산물 개발을 통해 섬 홍보에 활용할 수 있을 것으로 판단된다. The Islands can be said to be a source of future growth with high potential for use as marine resources, historical and cultural values, and territorial important assets. Accordingly, Jeollanamdo has established and is promoting the 10-year plan (2015-2024) for cultivating the “Islands that people hope to visit” in order to develop resources for comparative advantage and to discover contents unique in the islands. In particular, the Korea Island Promotion Agency was opened in Mokpo to conduct systematic research and research on the islands and to promote policy establishment in 2021. Geumdangdo in Wando and Geumhodo in Jindo were selected as part of the “Islands that people hope to visit” project in 2021, and research was carried out to find the comparative advantages of the islands. As a result of reviewing the results that have been promoted since 2015, it was found that the island area is rich in germanium and selenium components useful to the human body. In this study, the content of germanium and selenium, which are functional substances in soil and agricultural and fishery products and the image of a clean Jeonnam island was developed through soil contamination analysis. This was carried out to contribute to the creation of added value by confirming the impression of a clean island in Jeollanamdo. The analysis method of germanium and selenium was analyzed using ICP-MS and ICP based on the soil contamination process test standards, food standards and past thesis data. The germanium content in soil and tidal flats was analyzed 1,795.6 μg/kg ∼ 3,103.3 μg/kg in Geumdangdo and 1,797.3 μg/kg ∼ 2,914.4 μg/kg in Geumhodo. Soils and tidal flat sediments have 2,470.9 and 2,124.6 μg/kg in Geumdangdo and 2,521.7 μg/kg and 1,797.3 μg/kg in Geumhodo with germanium contents, respectively. Selenium content in soil and tidal flats was analyzed as 79.1 μg/kg ∼ 517.6 μg/kg in Geumdangdo and 214.4 μg/kg ∼ 978.2 μg/kg in Geumhodo. The average content were analyzed as 359.5 μg/kg in Geumdangdo soil and 146.9 μg/kg in tidal flats, and the average values ​​of Geumhodo were 609.6 μg/kg in soil and 214.4 μg/kg in tidal flats. It was analyzed that the soil content was higher than that of the tidal flat. The germanium component in agricultural and marine products was analyzed as high in Geumhodo bellflower(388.2 μg/kg) and deodeok (218.0 μg/kg), followed by Geumdangdo gastropods(181.8 μg/kg). As a result of the analysis of soil pollution in Geumdangdo and Gumhodo, the clean environment is maintained within the soil pollution concern standard (region 1), and it is judged that added value can be created using this. Geumhodo deodeok and bellflower have higher germanium content than other agricultural products. Geumdangdo Scallop has high selenium content and also contains germanium. Therefore, it is judged that it can be used to promote the islands through the development of excellent agricultural and fishery products using the aforementioned resources.

「가고 싶은 섬」의 갯벌과 토양환경 특성(Ⅴ) - 안마도, 선도 중심으로 -

유휘선 ( Yoo Hwi-sun ),양정고 ( Yang Jung-go ),박송인 ( Park Song-in ),박혜지 ( Park Hea-jin ),김수진 ( Kim Su-jin ),김경수 ( Kim Kyung-su ) 한국도서(섬)학회 2021 韓國島嶼硏究 Vol.33 No.4

섬은 관광, 생태, 문화 자원의 풍부하여 미래 성장 동력으로써 가치와 중요성이 커지고 있다. 전라남도는 섬들의 비교우위자원을 집중 개발하여 「가고 싶은 섬」가꾸기 10개년 계획(2015 ~ 2024)을 수립하여 추진하고 있으며, 2020년 「가고 싶은 섬」으로 선정된 영광 안마도와 신안 선도가 선정되었다. 이에 본 연구는 섬의 농ㆍ수산물의 우수성에 대한 과학적 자료 확보를 위해 기능성물질 게르마늄(Ge)과 셀레늄(Se) 함유량을 확인하고, 조사ㆍ분석한 결과 나타난 농수산물을 통해 부가가치 창출에 기여하고자 수행하였다. 게르마늄과 셀레늄의 분석방법은 수소화 발생법을 이용하여 ICP-MS로 분석하였고 토양의 게르마늄의 함량분포는 안마도 1.997 mg/kg ∼ 7.620 mg/kg, 선도 4.997 mg/kg ∼ 22.652 mg/kg이며, 갯벌의 함량 분포는 안마도 4.905 mg/kg ∼ 9.719 mg/kg, 선도 2.621 mg/kg ∼ 15.127 mg/kg으로 분석되었다. 안마도는 갯벌이 토양보다 높은 함량을 보였으며 선도는 토양과 갯벌에서 유사한 함량을 보였다. 선도의 경우 국내 다른 지역보다 토양의 게르마늄 함량이 높았는데 이는 변성암으로 구성된 지질학적 특성으로 판단된다. 토양의 셀레늄 함량분포는 토안마도 0.119 mg/kg ∼ 0.488 mg/kg, 선도 0.184 mg/kg ∼ 0.482 mg/kg이며 갯벌은 안마도 0.226 mg/kg ∼ 0.326 mg/kg, 선도 0.093 mg/kg ∼ 0.220 mg/kg으로 분석되었다. 농수산물 중의 게르마늄 성분은 안마도 쑥에서 29.399 μg/kg으로 가장 높은 농도를 보였으며, 안마도 파래 25.333 μg/kg, 선도 고시래기 23.556 μg/kg 순으로 나타났다. 토양 및 갯벌과 농·수산물의 게르마늄 함량을 비교한 결과 쑥, 파래, 굴, 꼬시래기 같은 수산물에서 높은 축적성을 나타내었다. 셀레늄은 안마도 바지락에서 483.628 μg/kg으로 가장 높았으며, 안마도 굴 388.279 μg/kg, 선도 게 218.447 μg/kg 순으로 높게 나타났다. 토양 및 갯벌과 농·수산물의 셀레늄 함량을 비교한 결과 농산물 보다 파래, 굴.게와 같은 수산물에서 높은 축적성을 나타내었다. 안마도의 모시풀은 게르마늄을 함유하고 있고 셀레늄도 식물에서 높은 함량이므로 관광 상품으로 가치가 있으며, 선도의 토양은 국내 다른 지역에 비해 게르마늄이 비교적 높은 함량이므로 재배방법의 개량을 통해 농작물의 게르마늄의 흡수율을 높일 수 있는 방법을 활용하여 관광 상품 개발이 가능할 것으로 판단된다. The islands of Anmado and Sundo are rich in tourism, ecology, and cultural resources, and are growing in value and importance as a future growth engine. By intensively developing the comparative advantage resources of the islands, a 10-year plan (2015 ~ 2024) for the “Islands that people hope to visit” has been established and is being promoted. Anmado in Yeonggwang-gun and Sundo in Shinan-gun were selected as the “Islands that people hope to visit” in 2020. Therefore, this study aims to contribute to the activation of the tourism industry and the creation of added value through the excellence of the islands by investigating and analyzing the functional substances in mud flats, soil, and agricultural and fishery products. Germanium and selenium in the soil, mud flats, and agricultural products were analyzed by ICP/MS using a hydrogen generation method. the mud flats showed a higher content than the soil in Anmado, and a similar content was analyzed in the soil and mud flat on Sundo. The ranges of germanium concentration were from 1.997 to 7.620 mg/kg in the soil on Anmado and 4.997 to 22.652 mg/kg in the soil on Sundo. In addition, the ranges of germanium concentration were from 4.905 to 9.719 mg/kg in the mud flats on Anmado and 2.621 to 15.127 mg/kg in the soil on Sundo. Sundo had a higher germanium content than other regions in Korea, which is judged to be due to the geology of metamorphic rocks. The ranges of selenium concentration were from 0.119 to 0.488 mg/kg in the soil on Anmado and 0.184 to 0.482 mg/kg in the soil on Sundo. While the ranges of selenium concentration were from 0.226 to 0.326 mg/kg in the mud flats on The Anmado and 0.093 to 0.220 mg/kg in the soil on Sundo. The germanium concentration in agricultural and fishery products were investigated the highest was shown in 29.399 μg/kg in wormwood from Anmado and second is 25.333 μg/kg in green laver/seaweed from Anmado. As a result of comparing the germanium content of soil and mud flats with agricultural and fishery products, it showed high accumulation in aquatic products such as wormwood, green laver, and oyster. The selenium concentration in agricultural products were investigated the highest was shown in 483.63 μg/kg in clams from Anmado and second is 388.279 μg/kg in oysters. As a result of comparing the selenium content of soil and tidal flats with agricultural and marine products, aquatic products such as green seaweed and oysters and crabs showed higher accumulation than agricultural products. The ramie of Anmado contains germanium and selenium is also high in the plant, so it is valuable as a tourist product. Since the soil of Seondo has a relatively high content of germanium compared to other regions in Korea, it is judged that tourism products can be developed by using a method that can increase the absorption rate of germanium in crops through improvement of cultivation methods.