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저온 대기압 플라즈마를 이용한 저장성병원균 Alternaria alternata 및 Botrytis cinerea 의 제어 효과
고선민 ( Go,S. M. ),김현승 ( H. S. Kim ),박미리 ( M. R. Park ),정래동 ( R. D. Jeong ) 강원대학교 농업생명과학연구원(구 농업과학연구소) 2018 강원 농업생명환경연구 Vol.30 No.3
Non-thermal atmospheric plasma (NTAP) has been shown to be effective for controlling postharvest fungi in vitro, but little is known about its mode of action, fungal response to NTAP, and the effect of its application on fresh produce. Non-thermal atmospheric plasma was evaluated for its in vitro and in vivo antifungal activity against Alternaria alternata and Botrytis cinerea on paprika. The treatment with NTAP treatment resulted in complete inhibition of mycelial growth of A. alternata, and B. cinerea, particularly at 1000 W for 90 s. The results of in vivo assays demonstrated that NTAP treatment for 90 s inhibited the growth of these fungal pathogens by approximately 55% (A. alternata) and 20% (B. cinerea). The color and hardness of paprika exhibited no significant changes during 7 days of storage after NTAP treatment. The mechanisms by which NTAP treatment decreased fungal growth on paprika were directly associated with the disruption of fungal cell membrane. These findings suggest that the application of NTAP is an effective antifungal treatment approach for preserving paprika.
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파프리카의 저장성 향상을 위한 플라즈마 살균 시스템 개발과 이의 효과 검정
김현승 ( Kim,H. S. ),고선민 ( S. M. Go ),정래동 ( R. D. Jeong ),권덕호 ( D. H Kwon ),박미리 ( M. R. Park ) 강원대학교 농업생명과학연구원(구 농업과학연구소) 2018 강원 농업생명환경연구 Vol.30 No.3
Paprika is an important agricultural export in Korea. However, paprika is not suitable for exporting over long distances as it does not store well. A major cause of damage to fresh agricultural produce, including paprika, is infection with pathogenic microorganisms that cause post-harvest diseases during storage and distribution. To address this problem, research is ongoing to develop a range of surface sterilization techniques. The recent confirmation of the sterilization effects of reactive oxygen species (ROS) generated from plasma discharge has attracted substantial research interest in applying the plasma technology to agriculture. Therefore, a plasma sterilization system was developed in this study to improve paprika storability. This system consists of an atmospheric-pressure plasma generator, a plasma controller, and a chamber for plasma treatment. To validate the system performance, the internal chamber temperature was measured; the volume of ozone, a strong oxidant that generates ROS, was also measured to compute the volume of all ROS generated through plasma discharge; based on this calculation, the concentration of ROS that can effectively deactivate microorganisms was identified. The sterilization effect of the present system was examined using Erwinia carotovora, the bacteria responsible for soft rot in paprika. Erwinia carotovora samples were treated with plasma for 30 and 60 s with 2.5 bar pressure and 500 W, 700 W, and 1000 W plasma power. The samples treated for 60 s showed a 100% sterilization effect. To then validate the effectiveness of the system in maintaining paprika freshness, paprika were treated at 1000 W plasma power for 30 and 60 s. As a result, browning was observed in the stem end of untreated paprika due to withering, whereas browning was delayed for 26 d in the stem end of plasma-treated paprika.
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담배가루이 유인용 LED 선발과 기주식물이 초기 유인력에 미치는 영향
권덕호,권민정,양다영,안율균,홍규현,박미리,Kwon, D.H.,Kwon, M.J.,Yang, D.Y.,Ahn, Y.K.,Hong, K.H.,Park, M.R. 국립한국농수산대학교 교육개발센터 2020 현장농업연구지 = Journal of practical agricultural resear Vol.22 No.2
시설 재배지 내 담배가루이의 밀도 예찰을 위하여 여러 종류의 LED등(청색등, 녹색등, 적색등, 백색등)에 대한 유인력을 평가하였다. 유인력 평가는 황색끈끈이트랩에 부착된 담배가루이의 포집 수를 대상으로 하였다. 평가용 케이지 내에서 기주식물이 없는 조건에서 유인력은 청색등(107.3±2.5마리) > 백색등(83.0±12.1마리) > 적색등(58±21.8마리) > 녹색등(39.7±8.1마리) 순서로 나타났다. 먹이를 주입한 상태에서 유인력은 청색등(52±17.4) > 적색등(38.7±5.8) > 녹색등(12.7±1.5) > 백색등(11.7±5.0) 순서로 관찰되었다. 두 실험 조건에서 모두 청색등에서 유인력이 가장 높은 것으로 나타났다. 기주식물을 넣었을 때 LED등 유인력이 감소하는 것을 확인하였는데, 백색등(85.9%) > 녹색등(68.1%) > 청색등(51.6%) > 적색등(33.3%) 순서로 나타나 기주식물이 LED 등 유인력 감소에 영향이 있음을 확인하였다. 상대적인 방제력 평가에서는 기주식물을 처리한 6시간 이후에 적색등(66.7%) > 청색등(48.5%) > 녹색등(31.9%) > 백색등(14.1%) 순으로 높았다(F<sub>3,8</sub> = 14.7, P = 0.001). 따라서 청색등은 초기 유인력이 매우 높은 특징을 보였고, 적색등은 기주식물의 투입에 영향을 받지 않아 담배가루이 유인에 적합성이 있음을 확인하였다. 야외 실증실험에서는 낮은 온도조건에 의해 높은 유인력은 관찰되지 않았지만, 청색등과 적색등에서 대조구 대비 높은 유인력이 관찰되었다. 향후 적색과 청색을 활용한 LED등은 시설재배지 내 담배가루이의 예찰에 활용할 수 있을 것으로 기대된다. Four LEDs (blue, green, red, and white light) were tested to identify the most attractive wave length to utilize as the forecasting tools for the B. tabaci in glass houses. Attractiveness was evaluated by the total number of the B. tabaci attached to a yellow sticky trap. In the condition of no host plant supplement, the attraction efficacy was ordered from high to low as blue light (107.3±2.5), white light (83.0±12.1), red light (58±21.8), and green light (39.7±8.1). In the supplement of the host plant, the attraction was observed in the order of blue light (52±17.4), red light (38.7±5.8), green light (12.7±1.5), and white light (11.7±5.0). In both experimental conditions, blue light showed the highest attraction. In terms of the host plant effect to LED attraction, it varied following as white light (85.9%), green light (68.1%), blue light (51.6%), and red light (33.3%). This result suggests that red light is the least affected by the host plant. In the evaluation of the relative control efficacy, it was determined following as red light (66.7%), blue light (48.5%), green light (31.9%) and white light (14.1%) (F<sub>3,8</sub> = 14.7, P = 0.001). Taken together, blue light had a very high initial attraction, and red light was revealed low attraction effect by the supplement of the host plant. In field demonstration experiments, a high attractive efficacy was not observed due to low-temperature conditions, but similar higher attractive efficacy was observed in blue and red lights compared to the control. The commercialization of LEDs using red and blue in the future is expected to provide important information regarding B. tabaci population density forecast in glass house.
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