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육우 수정란의 간역동결 및 융해방법에 관한 연구 1 . PMSG , HCG 투여가 가토 과배란 유기에 미치는 영향
김중계(J . K . Kim),김철균(C . K . Kim),이규훈(K . H . Lee),김동철(D . C . Kim),강민수(M . S . Kang) 한국축산학회 1988 한국축산학회지 Vol.30 No.9
Effects of the injection levels of PMSG and HCG and injection time on the induction of superovulation of rabbits were determined. In experiment 1 adult female rabbits were intramuscularly injected with 200 IU, 50 IU per day for consecutive 2 days (100 IU) or 100 IU of PMSG, respectively. HCG were injected to all rabbits 48 hrs after the PMSG, In experiment 2 rabbits were injected 100 IU of PMSG and 100 IU HCG(48 hrs after the PMSG) at the stage A(+), B(++) and C(+++); divided according to the degree of vaginal redness. Rabbits were allowed to mate and their reproductive organs were surgically removed 48 to 50 hours after the mating. The size of ovaries, the number of follicles and hemorrhagic follicles, ovulation points and the recovery of embroyos were determined, The results are summarized as follows: 1. The number of follicles (hemorrhagic follicles) obtained in rabbits injected with 200 IU, 50 IU twice or 100 IU of PMSG was 21.5(42.2), 22.7(10.8) or 20.9(16.2), respectively. 2. The size of ovaries was not different between rabbits injected with 100 IU of PMSG at vaginal stage A, B and C. However, the number of follicles (hemorrhagic follicles) were greater in rabbits injected at stage C than in those injected at stage A or B. 3. The number of ovulation points (recovery rate of embryos) in rabbits injected with 200 IU, 50 IU twice or 100 IU of PMSG was 23.2(94%), 16.3(81%) or 22.1(86%), respectively. 4. The number of ovulation points (recovery rate of embryos) in rabbits injected with 100 IU of PMSG at vaginal stage A, B or C was 32.4(91%), 18.0(84%.) or 9.8(76%), respectively. 5. The results indicate that the single injection of 100 IU PMSG at vaginal stage A was the best in terms of the follicle quality, the ovulation points and the recovery of embryos.
육우 수정란의 간역동결 및 융해방법에 관한 연구 3 . Sucrose 첨가한 glycerol 희석액에 간역동결 및 융해방법이 가토수정란 생존율에 미치는 영향
김중계(J . K . Kim),김철균(C . K . Kim),강만종(M . J . Kang),김영훈(Y . H . Kim),강민수(M . S . Kang) 한국축산학회 1988 한국축산학회지 Vol.30 No.10
This study was conducted to simplify the method for glycerol addition (a single step; 1S and 3-step addition; 3S) and diluent with 10% sucrose, and the method for freezing. Survival rates of rabbit embryos cultured after frozen in a liquid nitrogen container with different methods and thawed at different temperatures were determined. The summarized results are the following. 1. The embryo survival rates when a cryoprotectant diluent containing 10% sucrose were 75.3% (1S) and 69.8% (3S) (p $lt;0.05). 2. The survival rate of embryos cultured after thawed in a water bath at 38℃ or 5℃ was 75.5 or 69.6%, respectively (p $lt;0.05). 3. The survival rate of embryos thawed and cultured after the embryos at room temperature were frozen in a liquid nitrogen down to -7℃ at the rate of 1℃/min and then 0.3℃ (1 F), 3∼5℃(2F) 15℃/min (3F) or frozen in the gas phase 2 - 5 mm above the liquid nitrogen for 5 min and then inside the liquid nitrogen (4F) was 78, 71, 62 or 63%, respectively (p $lt;0.05). 4. The above results indicate that 1S (vs 3S), thawing at 38℃ (vs 5℃) and 1F (vs the others) showed higher embryo survival rates. However, the rapid freezing methods (2F, 3F and 4F) was considered relatively good.
육우 수정란의 간역동결 및 융해방법에 관한 연구 4 . 내동제에 sucrose 첨가시 간역동결과 식빙방법이 FDA test 에 의한 가토수정란 생존율에 미치는 영향
김중계(J . K . Kim),김철균(C . K . Kim),강만종(M . J . Kang),장덕지(D . J . Chang),김승호(S . H . Kim) 한국축산학회 1988 한국축산학회지 Vol.30 No.10
Effects of simplified procedures of freezing in a liquid nitrogen container and seeding on rabbit embryo survival rates were determined using the FDA test. The results found are summarized as follows: 1. When a single-step addition and removal of a cryoprotectant and a diluent, both containing 10% sucrose was used, seeding with a pincette resulted in a lower FDA test score (2.8) than that without seeding (3.2), but there was no significant differences. 2. Test score was 3.1, 3.2, 3.3 or 3.0 for embryos frozen in a liquid nitrogen container at the rate of 0.3℃, 3^5℃, 15℃/min or frozen in the gas phase 2∼5 mm above the liquid nitrogen and then inserted them into the liquid nitrogen, respectively. No significant differences were found between the freezing methods. 3. The FDA test was considered to be a simple and inexpensive procedure, as compared to the conventional test of embryo survival after culturing in CO₂ incubator.