http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
Transgenic 생쥐생산에 있어서 미세주입시기 및 외래유전자의 농도가 삽입빈도에 미치는 영향
한용만(Y . M . Han),이철상(C . S . Lee),강만종(M . J . Kang),유대열(D . Y . Yu),이경광(K . K . Lee) 한국축산학회 1990 한국축산학회지 Vol.32 No.6
The present study was undertaken in an attepmt to improve the efficiency of integration frequency in transgenic animals. In order to examine the effect of microinjection time on integration frequency, foreign DNA was microinjected into the male pronucleus of each fertilized egg at 2hr intervals from 21 to 27hr after HCG injection. The results revealed that the overall integration efficiency was somewhat higher in the group of 23-25hr than in the other groups. Wealso investigated on the effect of DNA concentrations(2, 4, 8 & 20ng/ul) on integration frequency, but differences between experimental groups were not statistically significant.
육우 수정란의 간역동결 및 융해방법에 관한 연구 4 . 내동제에 sucrose 첨가시 간역동결과 식빙방법이 FDA test 에 의한 가토수정란 생존율에 미치는 영향
김중계(J . K . Kim),김철균(C . K . Kim),강만종(M . J . Kang),장덕지(D . J . Chang),김승호(S . H . Kim) 한국축산학회 1988 한국축산학회지 Vol.30 No.10
Effects of simplified procedures of freezing in a liquid nitrogen container and seeding on rabbit embryo survival rates were determined using the FDA test. The results found are summarized as follows: 1. When a single-step addition and removal of a cryoprotectant and a diluent, both containing 10% sucrose was used, seeding with a pincette resulted in a lower FDA test score (2.8) than that without seeding (3.2), but there was no significant differences. 2. Test score was 3.1, 3.2, 3.3 or 3.0 for embryos frozen in a liquid nitrogen container at the rate of 0.3℃, 3^5℃, 15℃/min or frozen in the gas phase 2∼5 mm above the liquid nitrogen and then inserted them into the liquid nitrogen, respectively. No significant differences were found between the freezing methods. 3. The FDA test was considered to be a simple and inexpensive procedure, as compared to the conventional test of embryo survival after culturing in CO₂ incubator.
육우 수정란의 간역동결 및 융해방법에 관한 연구 3 . Sucrose 첨가한 glycerol 희석액에 간역동결 및 융해방법이 가토수정란 생존율에 미치는 영향
김중계(J . K . Kim),김철균(C . K . Kim),강만종(M . J . Kang),김영훈(Y . H . Kim),강민수(M . S . Kang) 한국축산학회 1988 한국축산학회지 Vol.30 No.10
This study was conducted to simplify the method for glycerol addition (a single step; 1S and 3-step addition; 3S) and diluent with 10% sucrose, and the method for freezing. Survival rates of rabbit embryos cultured after frozen in a liquid nitrogen container with different methods and thawed at different temperatures were determined. The summarized results are the following. 1. The embryo survival rates when a cryoprotectant diluent containing 10% sucrose were 75.3% (1S) and 69.8% (3S) (p $lt;0.05). 2. The survival rate of embryos cultured after thawed in a water bath at 38℃ or 5℃ was 75.5 or 69.6%, respectively (p $lt;0.05). 3. The survival rate of embryos thawed and cultured after the embryos at room temperature were frozen in a liquid nitrogen down to -7℃ at the rate of 1℃/min and then 0.3℃ (1 F), 3∼5℃(2F) 15℃/min (3F) or frozen in the gas phase 2 - 5 mm above the liquid nitrogen for 5 min and then inside the liquid nitrogen (4F) was 78, 71, 62 or 63%, respectively (p $lt;0.05). 4. The above results indicate that 1S (vs 3S), thawing at 38℃ (vs 5℃) and 1F (vs the others) showed higher embryo survival rates. However, the rapid freezing methods (2F, 3F and 4F) was considered relatively good.
사람 성장호르몬이 유선조직에서 특이적으로 발현되는 형질전환 생쥐의 개발
유대열(D . Y . Yu),이철상(C . S . Lee),강만종(M . J . Kang),한용만(Y . M . Han),이경광(K . K . Lee) 한국축산학회 1993 한국축산학회지 Vol.35 No.1
In an attempt to develop transgenic animals producing biologically active proteins into the milk., expression vectors (pC and pCS) were constructed. The pC vector consists of the promoter 2.8-kb DNA fragment of rat ?-casein gene and pSP70 plasmid. The pCS vector contains the SV40 poly(A) sequences in the multiple cloning sites of pC vector. Human growth hormone gene was then inserted into the cloning site of each vector, which was designated to pChGH and pCShGH. The plasmids were injected into one-cell pronuclear mouse embryos as a purified Xhol/Hpal fragments containing no procaryotic sequences. The injected embryos were implanted into pseudopregnant females, and 117 mice were born. The live of them were identified as being transgenic (ChGH line: 2 mice, CShGH line: 3 mice) by diagnostic Southern blot hybrization with human growth hormone gene. Among them, three transgenic mice transmitted the DNAs integrated on their chromosomes to their progenies and secreted human growth hormone in milk at the level of 160 ng/㎖ (ChGH-2-2 line), 80 ng/㎖ (CShGH-1 line), and 2-4 ng/㎖ (CShGH-3 line), respectively. The above cases show that no human growth hormone was detected in the serum, and these results suggest that the inserted gene is specifically expressed in the mammary gland of the transgenic mice by the regulation of the β-casein promoter.