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성준용(Jun-Yong Sung),변재민(Jae-Min Byun),노명찬(Myung-Chan Roh),김성훈(Sung-Hoon Kim),김중배(Joon-Bae Kim),한민홍(MinHong Han) 대한전자공학회 2010 대한전자공학회 학술대회 Vol.2010 No.6
We have developed an obstacle detection method using two cameras arranged vertically inside a vehicle. One camera is installed horizontally toward the front of a vehicle while another camera is installed, tilted toward the road surface, at a position which is higher than the first camera. From each image obtained from the two cameras, the farthest obstacle distance from the vehicle is calculated, and their calculated distance values are compared. If there exists a substantial difference in distance values, an obstacle is assumed to exist protruding from the road surface, otherwise no obstacle exists. The method is in use satisfactorily for adapted cruise control(ACC) purpose of a fully autonomous vehicle.
Cordyceps scarabaeicola KEFC-C252의 균사체 배양에 의한 수용성 색소의 생산과 색소의 항돌연변이 효과
이현우,손준형,최종환,예병일,신운섭,김중배,김현원 한국산업미생물학회 2000 한국미생물·생명공학회지 Vol.28 No.2
Cordyceps scarabaeicola KEFC-C252 균사체의 배양으로 생산되는 천연색소의 생산조건 및 색소의 정제방법을 조사하였으며, 분리된 색소의 항돌연변이 효과를 조사하였다. 색소의 생산에 적합한 배지는 1.5% sucrose, 2.5% yeast extract, 초기 pH5.5였다. C. scarabaeicola KEFC-C252를 26℃에서 108시간 배양한 후에 색소생산이 최대에 달하였으며, 최적조건에서 C. scarabaeicola KEFC-C252는 1.2 g/liter의 색소를 생산하였다. 색소는 ethylacetate 추출, 산침전 및 결정화과정을 거치면서 정제하여 황색의 육각기둥 모양의 결정을 얻었다. 분리된 색소는 용액의 pH변화에 따라 색상이 변하는 특성을 나타내었는데 알칼리성 용액에서 청색, 산성 용액에서 붉은색을 나타내었다. 분리된 색소는 4-NQO로 유도된 돌연변이를 억제하는 항돌연변이원성 물질이었다. 또 분리된 색소는 Salmonella typhimurium TA98과 TA100에서 자발적 돌연변이를 감소시켰다. Cultural conditions for the production of water-soluble pigment from mycelial culture of Cordyceps scarabaeicola KEFC-C252 and antimutagenic activity of the pigment were investigated. To obtain the maximum productivity of the pigment from mycelial culture of C. scarabaeicola KEFC-C252, the optimized medium was made with 1.5% sucrose, 2.5% yeast extract and initial pH 5.5. C. scarabaeicola KEFC-C252 was cultivated to reach the maximum concentration of the pigment at 26℃ for 108 hrs. C. scarabaeicola KEFC-C252 produced about 1.2 g/liter pigment under the optimized condition. The pigment was isolated from the culture filtrate by ethylacetate extraction, acidic precipitation and crystallization. The isolated pigment was scarlet hexagonal column crystal, and the color of the pigment was changed according to pH of the solution. The pigment showed violet in the alkaline water but showed red color in the acidic water. The pigment showed inhibitory activity against mutagenic activity induced by 4-nitroquinoline N-oxide. Furthermore, the pigment showed inhibitory activity against spontaneous mutation on Salmonella typhimurium TA98 and TA100.