LC-MS/MS를 이용한 수산물 중 니트로빈의 정량분석법 개발 및 검증

김주혜,신다솜,강희승,정지윤,이규식,Kim, Joohye,Shin, Dasom,Kang, Hui-Seung,Jeong, Jiyoon,Rhee, Gyu-Seek 한국식품위생안전성학회 2018 한국식품위생안전성학회지 Vol.33 No.2

본 연구에서는 우리나라 식품공전에서 불검출 물질로 관리하고 있는 니트로빈(nitrovin)에 대해 고감도 정량 정성분석이 가능한 LC-MS/MS를 적용하여 적합한 분석법을 제시하고자 하였다. 수산물 시료는 아세토니트릴/물로 추출하고 아세토니트릴 포화 헥산으로 지방을 제거하여 고상추출 카트리지를 적용하여 정제하였다. 분석물질은 전기분무이온화방법의 positive mode에서 이온화하여 MRM 조건을 확립하여 분석하였다. 개선된 시험법은 CODEX CAC/GL-71 가이드라인에 따라서 정확성, 정밀성, 직선성, 정량한계에 대한 검증을 통하여 유효성을 확인하였다. 본 실험에서의 정량한계는 0.001 mg/kg 수준이며, 정량한계를 포함하는 표준시료에서 얻어진 검량선의 상관계수($r^2$)는 0.985 이상으로 시험법의 직선성이 유효함을 판단할 수 있었다. 또한, 수산물(넙치, 장어 및 새우) 시료에 대한 니트로빈의 평균 회수율과 변동 계수는 72.1~122%, 2.9~16.9%로 확인되어 정확성 및 정밀성이 CODEX가이드라인에 부합하였다. 따라서, 개선된 니트로빈 정량분석법은 수산물 중 니트로빈을 분석하는데 적합하며, 니트로빈에 대한 지속적인 잔류실태조사에 활용되어 수산물 중 니트로빈의 안전관리에 기여할 것으로 판단된다. The objective of this study was to develop a sensitive method for the identification and determination of nitrovin in fishery products by using a solid-phase extraction (SPE), as performed with a liquid chromatography-tandem mass spectrometry (LC-MS/MS). The samples were extracted with a mixture of acetonitrile and water, and were then defatted with acetonitrile saturated hexane, after which further clean-up was accomplished with SPE on the hydrophilic-lipophilic balance (HLB) cartridges. The analytes were subsequently ionized in the positive mode of an electrospray ionization (ESI), and where thereby detected in a process of multiple reaction monitoring (MRM). The linearity (expressed as correlation coefficients) of the matrix calibration curves was > 0.985. The limit of the quantification for the nitrovin was measured at 0.001 mg/kg. The accuracy (expressed as average recovery) was noted between 72.1 and 122%. The precision (expressed as coefficient variation) was noted from 2.9 to 16.9%. According to the CODEX CAC/GL-71 guideline accuracy, precision, linearity, and limit of detection were determined in three matrices (which were flatfish, eel and shrimp). The proposed method was suitable for analyzing the associated nitrovin residues. This application and result can also be a factor to contribute to the non-detection drugs management in fishery products.

고속도로 화물차의 군집주행이 교통류에 미치는 영향 및 전용차로 효과 연구

김주혜,이영인,KIM, Joohye,Lee, YoungIhn 한국ITS학회 2020 한국ITS학회논문지 Vol.19 No.5

화물차 군집주행이 화물교통량 증가에 따른 문제를 해결할 수 있는 대안으로 떠오르는 가운데, 화물차 군집주행이 교통류에 미치는 영향과 군집주행 전용차로 도입 효과를 분석하는 연구를 수행하였다. 연구결과, 교통량이 많고 화물차 혼입률이 높아질수록 화물차의 군집비율이 높아지고 최대 군집형성 규모가 증가함에 따라 군집주행으로 인한 평균속도 증가효과가 더 크게 나타났다. 하지만, 진출입 등으로 대기행렬이 발생한 경우에는 평균속도 증가효과는 기대하기 어려우며 오히려 처리량이 감소할 수 있는 것으로 분석되었으므로 접속부의 용량 확보 등 접속도로와 연계된 교통운영 전략이 필요하다는 점을 확인할 수 있었다. 군집 전용차로의 효과는 자율주행화물차 비율(MPR)이 100%가 되어 한 개 차로가 완전히 자율주행화물차만으로 운영될 경우에만 미 도입 시 보다 긍정적 효과를 얻을 수 있었다. 본 연구는 국내 도로·교통 조건을 시나리오에 반영하여 군집주행의 영향을 종합적으로 분석하였으므로 구간별, 시간대별로 군집주행 및 전용차로의 영향을 가늠해 볼 수 있으며, 이는 향후 화물차 군집주행을 고려한 도로 운영전략 수립이나 관리에 기초자료로 사용할 수 있을 것으로 판단된다. Considering the need for an infrastructure-level review, this study analyzed the impact of truck platooning on freeway traffic flow and the effect of dedicated lanes based on domestic road and traffic conditions. According to the study, the higher traffic volume and truck ratio, the higher ratio of platoons and the greater size of platoons are formed, which results in greater effect of increasing the average speed of the network. Therefore, the routes with heavy traffic and heavy cargo traffic could be positively considered for truck platooning. However, the analysis showed that the effect of increasing the average speed of the entire network is difficult to expect in the event of a queue due to entry and exit, and that the overall network's throughput could be reduced. Therefore, traffic operation strategies associated with the access road, such as securing capacity of the connection, are needed to maximize the effect of truck platooning. When it comes to the effect of dedicated lane, it could have a positive effect only if one lane was fully operated by automated trucks under the condition of 100% MPR, which allowed positive effects in all aspects, such as higher average speed, throughput, and reduced conflict rates.

다이오드 검출기를 이용한 초소형 X선관(Miniature X-ray Tube)의 반가층 측정

김주혜,안소현,오윤진,지윤서,허장용,강창무,서현숙,이레나,Kim, Ju-Hye,An, So-Hyeon,Oh, Yoon-Jin,Ji, Yoon-Seo,Huh, Jang-Yong,Kang, Chang-Mu,Suh, Hyunsuk,Lee, Rena 한국의학물리학회 2012 의학물리 Vol.23 No.4

X선은 방사선 진단과 치료 분야에 있어서 다양하고 광범위하게 이용되고 있으며, 최근에는 방사선 치료용 초소형 X선관이 개발되었다. 초소형 X선관은 조사 목적 부위에 직접 삽입하여 사용되므로 제작 시준기에 따라 다양한 각도로 X선 조사가 가능하고, 검사 목적 외의 환자 피폭선량을 최소화한다. 이러한 초소형 X선관의 장점을 이용해서 X선 영상을 획득하는데 적용한다면 X선 진단 분야의 새로운 장을 열 것으로 기대된다. 하지만 초소형 X선관은 본래 치료용으로 설계되었기 때문에 진단용 장비에 적합한 시준기, 필터(added filter) 등이 필요하다. 따라서 자체 제작한 시준기와 필터를 적용하여 초소형 X선관의 빔 특성이 진단용에 적합한지 평가 하였고, 이를 위해서 다이오드 검출기를 이용하여 반가층을 측정하고 측정의 가능성을 평가하였다. 본 연구에서는 Si PIN Photodiode type인 Piranha 검출기(Piranha, RTI, Sweden)를 사용하여 필터 적용 유무에 따른 초소형 X선관의 반가층을 측정하고, 알루미늄 필터를 사용한 측정을 통하여 Piranha 검출기의 반가층 측정의 정확성을 평가하였다. 측정 결과에 따르면 초소형 X선관의 반가층은 필터의 장착에 따라 약 1.9배 증가하여 진단용 방사선 발생 장치의 적합성을 확인하였다. Piranha 검출기의 반가층 자동 측정값은 필터를 미장착한 경우에 실제 반가층 측정값에 비해 50% 높게 측정되어 적용이 불가능하나, 필터를 장착한 경우에는 실제 반가층 측정값과 약 15%의 차이로 감소되었다. 따라서 진단용 필터를 적용했을 경우는 Piranha 검출기의 반가층 자동측정이 가능하여 kV-X선 특성평가를 수월하게 수행할 것으로 기대된다. The X ray has been widely used in both diagnosis and treatment. Recently, a miniature X ray tube has been developed for radiotherapy. The miniature X ray tube is directly inserted into the body irradiated, so that X rays can be guided to a target at various incident angles according to collimator geometry and, thus, minimize patient dose. If such features of the miniature X ray tube can be applied to development of X ray imaging as well as radiation treatment, it is expected to open a new chapter in the field of diagnostic X ray. However, the miniature X ray tube requires an added filter and a collimator for diagnostic purpose because it was designed for radiotherapy. Therefore, a collimator and an added filter were manufactured for the miniature X ray tube, and mounted on. In this study, we evaluated beam characteristics of the miniature X ray tube for diagnostic X ray system and accuracy of measuring the HVL. We used the Si PIN Photodiode type Piranha detector (Piranha, RTI, Sweden) and estimated the HVL of the miniature X ray tube with added filter and without added filter. Through an another measurement using Al filter, we evaluated the accuracy of the HVL obtained from a direct measurement using the automatic HVL calculation function provided by the Piranha detector. As a result, the HVL of the miniature X ray tube was increased around 1.9 times with the added filter mounted on. So we demonstrated that the HVL was suitable for diagnostic X ray system. In the case that the added filter was not mounted on, the HVL obtained from use of the automatic HVL calculation function provided by Piranha detector was 50% higher than the HVL estimated using Al filter. Therefore, the HVL automatic measurement from the Piranha detector cannot be used for the HVL calculation. However, when the added filter was mounted on, the HVL automatic measurement value using the Piranha detector was approximately 15% lower than the estimated value using Al filter. It implies that the HVL automatic measurement can be used to estimate the HVL of the miniature X ray tube with the added filter mounted on without a more complicated measurement method using Al filter. It is expected that the automatic HVL measurement provided by the Piranha detector enables to make kV-X ray characterization easier.

[<SUP>14</SUP>C]Butachlor를 이용한 호기성 토양대사 시험법 확립

김주혜(Ju-Hye Kim),김종환(Jong-Hwan Kim),김대욱(Dae-Wook Kim),이봉재(Bong-Jae Lee),김찬섭(Chan Sub Kim),임양빈(Yangbin Ihm),서종수(Jong-Su Seo) 한국농약과학회 2014 농약과학회지 Vol.18 No.4

[<SUP>14</SUP>C]Butachlor의 벼에 대한 흡수 및 대사

김주혜(Ju-Hye Kim),김종환(Jong-Hwan Kim),김대욱(Dae-Wook Kim),이봉재(Bong-Jae Lee),김찬섭(Chansub Kim),임양빈(Yangbin Ihm),서종수(Jong-Su Seo) 한국농약과학회 2015 농약과학회지 Vol.19 No.3

학교차원의 장애이해교육이 일반 초등학생의 장애 학생에 대한 태도 변화에 미치는 영향

김주혜 ( Joo Hye Kim ) 한국특수아동학회 2012 특수아동교육연구 Vol.14 No.4

COVID-19로 인한 소득변화를 경험한 중년남성 근로자의 건강행태 및 정신건강: 자영업자와 임금근로자 비교

김주혜(Juhye Kim),허경화(Kyunghwa Heo),정진욱(Jinwook Jung) 한국직업건강간호학회 2023 한국직업건강간호학회지 Vol.32 No.2

Purpose: This study aimed to understand how changes in income due to the COVID-19 pandemic have affected the health behavior and mental health status of self-employed individuals. Methods: We compared the health behavior and mental health status of regular wage workers and self-employed individuals with no change in income, with that of self-employed individuals with reduced income due to the spread of COVID-19. Results: Smoking status, average amount of smoking per day, changes in the amount of smoking and drinking due to COVID-19, drinking frequency per year, monthly binge drinking experiences, subjective stress, and suicidal thoughts experienced by self-employed individuals with decreased income were not only higher than those of wage workers and self-employed individuals with maintained income, but their happiness index was also lower than the latter group. Conclusion: This study suggests that the change in total household income due to COVID-19 adversely affects the health behavior and mental health status of self-employed individuals. However, COVID-19-related policies focus only on economic loss compensation, and the health behavior and mental health management for self-employed individuals is insufficient. Therefore, it is necessary to establish policies for health behavior and mental health management of self-employed individuals.

특수학교의 학교시설 개방 및 시설복합화를 통한 지역사회 연계

김주혜(Kim, Joohye),이영선(Lee, Youngsun) 한국교육시설학회 2021 敎育施設 Vol.28 No.5

통합학급교사와 특수교사의 협력프로그램이 교수행동 및 수업참여행동에 미치는 영향

김주혜 ( Joo Hye Kim ),박은혜 ( Eun Hye Park ),이경순 ( Kyoung Soon Lee ) 한국특수아동학회 2008 특수아동교육연구 Vol.10 No.1

고농도 Galactose로부터 에탄올을 생산하는 Saccharomyces cerevisiae 균주의 육성

김주혜 ( Ju Hye Kim ),윤민호 ( Min Ho Yoon ) 한국응용생명화학회 2011 Journal of Applied Biological Chemistry (J. Appl. Vol.54 No.1

A galactose-fermenting yeasts, Saccharomyces cerevisiae No. 9, was selected by screening their abilities to produce carbon dioxide gas when grown on galactose. The selected strain, No. 9 and the reference strains NRRL Y-1528 which was exceptionally resistant to high concentration of substrate, were acclimated on sugars such as glucose, mannose, and galactose, and then their ethanol productivities were investigated during fermentation on these three carbon sources. Ethanol productivity of the strain No. 9 reached to the maximum levels after 18 h of fermentation and the ethanol yield was from 36 to 38% when presented as [EtOH]max/ [Sugar]ini (g/g), regardless of the conditions of acclimation. From the results obtained by acclimation and fermentation, it was concluded that the ethanol yields from galactose were not affected by the sugars acclimated. Improvements of the strain S. cerevisiae No. 9 were attempted to increase the fermentation efficiency and/or ethanol yields on high concentration of substrate by the conventional mutation methods employing methanesulfonic acid, ethyl ester (EMS). Mutants, Mut-5 (SJ1-40), -17 (LK4-25) and -24 (LK2-48) fermented galactose at the concentration of 20% in the levels of higher 39.9~51.6% than the mother strain, No. 9, however, their ethanol yields never exceeded those of the reference strain.