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김영환 ( Y . H . Kim ) 한국축산학회 1989 한국축산학회지 Vol.31 No.8
The sodiumdodecylsulfate-polyacrylamide gel electrophoresis(SDS-PAGE) was used to determine the nonmeat protein contents(Isolated soy protein: ISP, Vital wheat gluten: VWG) in cooked meat/non-meat protein blends. Differentiation and identification of meat species by isoelectric focusing(IEF) in polyacrylamide gel (pH range 3-10) were studied. The fresh meat samples from 2 meat species(beef, pork) were compared. Differential scanning calorimeter(DSC) was applied to analyze the mixing ratio of pork and beef in meat blends (mixing ratios of beef to pork were 1:0.3, 0.6 and 1.0) by studying the heat denaturation properties(Transition temperature and Enthalpy) of stroma proteins. The results obtained were summarized as follows; 1. SDS-PAGE patterns showed protein bands in similar positions, so this method was not reliable for the identification of meat species. Protein from the different meat species may differ little in intensity or sharpness of the bands. However, SDS-PAGE method suggested that meat and non-meat protein in meat blends can be successfully identified and quantitated. 2. IEF band patterns from meat proteins of the different species showed the most distinct differences. 3. The regression equation of the line for peak area ratio(Y) versus protein concentration ratio(X) of ISP/beef protein was found to be Y=1.2860X + 0.0017(r=0.9943). For VWG content, corresponding line was Y=O.7380X +0.0058(r=0.9920). 4. In the case of pork, the regression equation of the line for peak area ratio(Y) versus protein concentration ratio(X) of ISP/pork protein was found to be Y=1.0227X -0.0003(r=0.9952). And the equation with VWG content was Y=0.5452X + 0.0092(r=0.9843). 5. Application of the above equations made the quantitation of ISP and VWG in a meat blend possible within 2% of actual content. 6. With DSC analysis of the mixture of pork and beef, the linear equations of the mixing ratio were calculated as Y=0.51X + 67.30(r=0.997) for transition temperature and Y=0.29X + 0.60(r=0.980) for enthalpy.
김영환(Y . H . Kim),박구부(G . B . Park),이무하(M . H . Lee),김일석(I . S . Kim) 한국축산학회 1987 한국축산학회지 Vol.29 No.9
The stacking sodium dodecylsulfate-acrylamide gel electrophoresis method was used to determine the non-meat protein content in chicken products. Densitograms of the selected protein band, denoted by SI, MI, used for the quantitative estimation of isolated soy protein (ISP) and milk protein (MP), respectively. The equation of the line of best fit fot peak area ratio (y) versus protein concentration ratio (X) of ISP/ chicken protein was found to be ; y = 1.5096X + 0.0163 (r=0.9318). And for MP content the corresponding line of best fit was y=1.3536X - 0.0112 (r = 0.9693). These protein content could be determined within 2% of the true value in the range of 10∼30% ISP and MP respectively.
기판 온도의 영향에 따른 펄스레이저 증착법으로 성장된 ZnO 박막의 발광 특성
김영환,김성일,Kim, Y.H.,Kim, S.I. 한국진공학회 2009 Applied Science and Convergence Technology Vol.18 No.5
펄스레이저 증착법으로 박막의 결함 생성을 최소화하여 우수한 발광 특성을 가지는 ZnO 박막 성장에 대한 연구를 수행하였다. 이를 위하여 기판 온도를 $400^{\circ}C$에서 $850^{\circ}C$까지 변화시켜 박막을 증착한 후 엑스선 회절법, 원자힘 현미경, photoluminescence (PL) 등을 사용하여 박막의 특성 변화를 분석하였다. 그 결과 ZnO 박막은 기판 온도에 관계없이 (0001) 사파이어 기판에 c-축 배향성을 가지며 성장하였음을 확인하였고 기판온도 $600^{\circ}C$에서 가장 조밀한 박막이 형성되면서 박막에 응력이 거의 걸리지 않고 결정성도 우수함을 확인하였다. PL 분석 결과 역시 $600^{\circ}C$에서 증착된 ZnO 박막이 UV 발광 피크의 반치폭 및 결함에 의한 가시영역에서의 발광 등을 고려했을 때 가장 뛰어난 특성을 보여주었다. 이와 같은 결과는 ZnO 박막의 발광 특성이 박막의 구조적 특성과 매우 밀접한 관계가 있음을 나타내며 또한 기판 온도가 매우 중요한 역할을 함을 나타낸다. 결론적으로 기판 온도 $600^{\circ}C$에서 우수한 UV 발광 특성을 가지면서 결함에 의한 가시영역 발광이 거의 나타나지 않는 ZnO 박막을 성장시킬 수 있었고 이러한 박막은 UV 광소자에 응용될 수 있을 것으로 생각된다. We investigated the growth of ZnO thin films with prominent emission characteristics through minimizing the formation of defects by using pulsed laser deposition (PLD). To do so, the ZnO films were deposited on sapphire(0001) substrates at the substrate temperature of $400-850^{\circ}C$ and then the variation of their structural and optical properties were analyzed by x-ray diffraction, atomic force microscope and photoluminescence. As a result, all ZnO films were grown with c-axis preferential orientation irrespective of the substrate temperature. However, the crystallinity and stress state were dependent on the substrate temperature and the ZnO film deposited at $600^{\circ}C$ showed the best surface morphology and crystallinity with nearly no strain. And also this film exhibited outstanding emission characteristics from the viewpoint of full width half maximum of UV emission peak as well as visible emission due to defects. These results indicate that the emission characteristics of the ZnO films are strongly related to their structural characteristics influenced by substrate temperature. Consequently, ZnO films with strong UV emission and nearly no visible emission, which are applicable to UV emission devices, could be grown at the substrate temperature of $600^{\circ}C$ by PLD.
RF 마그네트론 스퍼터링법에 의한 a-축 배향 $YBa_2Cu_3O_{7-\delta}$박막의 제조
이재준,김영환,신진,이경희,최상삼,한택상,Lee, J.J.,Kim, Y.H.,Shin, J.,Lee, K.H.,Choi, S.S.,Hahn, T.S. 한국재료학회 1994 한국재료학회지 Vol.4 No.4
a-측으로 배향된 $YBa_2Cu_3O_{7-\delta}$ 고온 초전도 박막을 $LaAIO_{3}$(100)단결정 기판에 이중 타게트 off-axis rf마그네트론 스퍼터링법으로 증착하였다. 박막은 기판온도(Ts)$590^{\circ}C$와 $680^{\circ}C$사이에서 단일공정으로 증착하는 one-step방법과, $590^{\circ}C$의 저온에서 a-축으로 배향된 YBCO박막(두께-30nm)을 면저 만들어 틀로 작용시킨 후 그 틀위에 나머지 부분을 기판온도를 승온하면서 증착하는 방법인 two-step방법 등 두 가지 방법을 사용하여 증착시켰다. one-step방법에서는 $T_s$가 증가함에 따라 감소하였으며, ($00 \ell$)피크는 증가하였다. Two-step방법으로 증착한 박막은 증착속도가 감소함에 따라 (h00)피크가 우세하게 나타났다. 박막의 미세구조는 a-축, c- 축 배향성이 혼재하여 핀홀과 같은 결함들이 생성되었다. 모든 경우 $T_s$가 감소함에 따라 a-축 배향성은 우세하였으나 전기적 특성은 저하되었고, 긴 전이온도 폭을 가졌다. A-axis oriened YBCO thin flims were grown on $LaAIO_{3}$ single crystal substrate by off-axis rf magnetron sputtering method. We used two kinds of process to get a-axis oriented fi1ms;one-step process and two-step process. In one-step process, films are grown in single step in which substrate temperature( $T_s$) is in the range of $590^{\circ}C$ to $680^{\circ}C$. On the other hand, in two step process a-axis oriented thin film templates i f about 30nm thickness is deposited at low temperature first, and subsequently films are grown at elevated temperature to the final thickness of about 100nm. In the case of one step process($T_s$ ~)$600^{\circ}C$), prefered a-axis orientation is dominant and Cu-rich phases segregate at the surface. Segregations decrease and ($00 \ell$) peaks increase upon increasing $T_s$. The films prepared by two step method appeared to have strong(h00) peaks as the deposition rate increased. Microstructure shows pin holes resulted from mixed phases of a-axis and c-axis oriented films. In both cases of one step and two step process, as TS decreases, prepared films show stronger a-axis orientation. However electrical properties of the films are depressed with lower $T_c$ and wider $\Delta T$ as $T_s$ decreases.
포스터 전시 : 담도,췌장 ; 신경섬유종증을 동반한 췌장암 1예
김영환 ( Y. H. Kim ),이태규 ( T. K. Lee ),김성수 ( S. S. Kim ),방춘상 ( C. S. Bang ),채현석 ( H. S. Chae ),한석원 ( S. W. Han ),이창돈 ( C. D. Lee ),최규용 ( K. Y. Choi ),정인식 ( I. S. Chung ),선희식 ( H. S. Sun ),박도호 대한소화기학회 2002 대한소화기학회 춘계학술대회 Vol.2002 No.-
<증례> 54세 남자 환자가 전신쇄약감과 현기증을 주소로 내원하였다. 과거력상 심방세동 있었으며 가족력상 특이 사항 없었다. 이학적 소견상 만성병색 소견을 보이고 결막이 창백해보였으며 두경부 검사와 흉부 청진상 특이 소견 없었다. 복부 촉진상 상복부의 경미한 압통이 있었고 복부와 등, 얼굴 피부에도 밀크 커피색 반점을 동반한 다양한 크기의 종괴들이 관찰되었다. 검사실 소견에서 Hemoglobin 7.5g/dl, Hematocrit 24.0% 백혈구수 9